Cancer cell membrane-coated nanogels as redox/pH dual-responsive drug carrier for tumor-targeted therapy

Techniques that increase the permeability of the cell membrane and transfer drugs or genes to cells have been actively developed as effective therapeutic modalities. Also, in line with the development of these drug delivery techniques, the establishment of tools to verify the techniques at the cellular level is strongly required. In this study, we demonstrated an optical imaging platform integrated with an ultrasound application system to verify the feasibility of safe and efficient drug delivery through the cell membrane using ultrasound-microbubble cavitation. To examine the potential of the platform, fluorescence images of both Fura-2 AM and propidium iodide (PI) to measure calcium flux changes and intracellular PI delivery, respectively, during and after the ultrasound-microbubble cavitation in the cervical cancer cell were acquired. Using the optical imaging platform, we determined that calcium flux increased immediately after the ultrasound-microbubble cavitation and were restored to normal levels, and fluorescence signals from intracellular PI increased gradually after the cavitation. The results acquired by the platform indicated that ultrasound-microbubble cavitation can deliver PI into the cervical cancer cell without irreversible damage of the cell membrane. The application of an additional fluorescent imaging module and high-speed imaging modalities can provide further improvement of the performance of this platform. Also, as additional studies in ultrasound instrumentations to measure real-time cavitation signals progress, we believe that the ultrasound-microbubble cavitation-based sonoporation can be employed for safe and efficient drug and gene delivery to various cancer cells.

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Polymerisation-induced self-assembly (PISA) as a straightforward formulation strategy for stimuli-responsive drug delivery systems and biomaterials: recent advances

Biomaterials Science ◽

10.1039/d0bm01406k ◽

2021 ◽

Vol 9 (1) ◽

pp. 38-50

Author(s):

Hien Phan ◽

Vincenzo Taresco ◽

Jacques Penelle ◽

Benoit Couturaud

Keyword(s):

Drug Delivery ◽

Block Copolymers ◽

Drug Release ◽

Self Assembly ◽

Drug Delivery Systems ◽

Amphiphilic Block Copolymers ◽

Stimuli Responsive ◽

Site Specific ◽

On Demand ◽

Redox Agents

Stimuli-responsive amphiphilic block copolymers obtained by PISA have emerged as promising nanocarriers for enhancing site-specific and on-demand drug release in response to a range of stimuli such as pH, redox agents, light or temperature.

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Folate-conjugated dually responsive micelles for targeted anticancer drug delivery

RSC Advances ◽

10.1039/c6ra01885h ◽

2016 ◽

Vol 6 (42) ◽

pp. 35658-35667 ◽

Cited By ~ 9

Author(s):

Lingling Zhao ◽

Yajuan Zhang ◽

Jia Shao ◽

Hongze Liang ◽

Haining Na ◽

...

Keyword(s):

Drug Delivery ◽

Drug Release ◽

Anticancer Activity ◽

Anticancer Drug ◽

Anticancer Drug Delivery ◽

Dual Responsive

Folate-conjugated dual-responsive micelles were developed, sustained and sensitive drug release from the drug loaded micelles was observed. Folate-targeted micelles showed higher anticancer activity and enhanced cellar uptake than non-targeted ones.

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Cell membrane based biomimetic nanocomposites for targeted therapy of drug resistant EGFR-mutated lung cancer

Nanoscale ◽

10.1039/c9nr05791a ◽

2019 ◽

Vol 11 (41) ◽

pp. 19520-19528 ◽

Cited By ~ 9

Author(s):

Pengying Wu ◽

Dongtao Yin ◽

Jiaming Liu ◽

Huige Zhou ◽

Mengyu Guo ◽

...

Keyword(s):

Lung Cancer ◽

Drug Resistance ◽

Targeted Therapy ◽

Cell Membrane ◽

Cancer Cell ◽

Egfr Mutation ◽

Drug Resistant ◽

Egfr Mutated

A cancer cell membrane-based biomimetic strategy was developed by loading doxorubicin and icotinib to overcome drug-resistance of EGFR-mutation lung cancer.

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Programmable shape-morphing microrobots for localized cancer cells treatment

10.21203/rs.3.rs-198740/v1 ◽

2021 ◽

Author(s):

Chen Xin ◽

Dongdong Jin ◽

Yanlei Hu ◽

Liang Yang ◽

Rui Li ◽

...

Keyword(s):

Drug Delivery ◽

Drug Release ◽

Targeted Drug Delivery ◽

Biological Applications ◽

Proof Of Concept ◽

Ph Responsive ◽

Solution Ph ◽

Shape Morphing ◽

On Demand ◽

Targeted Drug

Abstract Microrobots have attracted great attentions due to their wide applications in microobjects manipulation and targeted drug delivery. To realize more complex micro/nano cargos manipulation (e.g., encapsulation and release) in biological applications, endowing microrobots with shapes adaptability with the environment is highly desirable. Here, designable shape-morphing microrobots (SMMRs) have been developed by programmatically encoding different expansion rate in a pH-responsive hydrogel. Combined with magnetic propelling, the shape-morphing microcrab (SMMC) is capable of performing targeted microparticle delivery, including gripping, transporting, and releasing through claws morphing. As a proof-of-concept demonstration, the shape-morphing microfish (SMMF) is designed to encapsulate drug (doxorubicin (DOX)) by closing mouth in phosphate buffer saline (PBS, pH~7.4) and release them by opening mouth in slightly acid solution (pH<7), which realize localized Hela cells treatment in an artificial vascular network. These SMMRs with powerful shape morphing capabilities and remote motion controllability provide new platforms for complex microcargos operation and on-demand drug release.

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Magnetic micropump embedded in contact lens for on-demand drug delivery

Micro and Nano Systems Letters ◽

10.1186/s40486-019-0101-x ◽

2020 ◽

Vol 8 (1) ◽

Author(s):

Cong Wang ◽

Jungyul Park

Keyword(s):

Magnetic Field ◽

Drug Delivery ◽

External Magnetic Field ◽

Drug Release ◽

Contact Lens ◽

Tear Film ◽

Check Valve ◽

Drug Diffusion ◽

On Demand ◽

The Magnetic Field

AbstractIn this paper, we report a thin magnetic micropump embedded in contact lens, which is capable of on-demand one-directional drug delivery. The proposed micropump can be actuated by the external magnetic field whenever needed without the need of battery. A micro check valve was integrated with the micropump for one-directional drug delivery from the micropump to the post-lens tear film. With actuation of the external magnetic field, the micro check valve is opened, and on-demand drug release can be realized. On the contrary, without an external magnetic field, the micro check valve is closed, and the undesired drug diffusion can be prevented. Through the control of the strength and the frequency of the magnetic field pulse, on-demand drug release and controlled dose can be realized.

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Rational design of drug delivery systems for potential programmable drug release and improved therapeutic effect

Materials Chemistry Frontiers ◽

10.1039/c9qm00178f ◽

2019 ◽

Vol 3 (6) ◽

pp. 1159-1167 ◽

Cited By ~ 2

Author(s):

Yuxun Ding ◽

Jinjian Liu ◽

Xue Li ◽

Linlin Xu ◽

Chang Li ◽

...

Keyword(s):

Drug Delivery ◽

Cancer Therapy ◽

Drug Release ◽

Therapeutic Effect ◽

Drug Delivery Systems ◽

Rational Design ◽

Delivery Systems ◽

Dual Responsive ◽

Ph Reduction

pH-Reduction dual responsive nanocarriers (DRNs) achieve programmable release of CA4 and CDDP in cancer therapy.

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Smart pH/Redox Dual-Responsive Nanogels for On-Demand Intracellular Anticancer Drug Release

ACS Applied Materials & Interfaces ◽

10.1021/acsami.6b01602 ◽

2016 ◽

Vol 8 (12) ◽

pp. 7729-7738 ◽

Cited By ~ 78

Author(s):

Hao Yang ◽

Qin Wang ◽

Shan Huang ◽

Ai Xiao ◽

Fuying Li ◽

...

Keyword(s):

Drug Release ◽

Anticancer Drug ◽

On Demand ◽

Dual Responsive

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Monitoring of “on-demand” drug release using dual tumor marker mediated DNA-capped versatile mesoporous silica nanoparticles

Chemical Communications ◽

10.1039/c7cc02752d ◽

2017 ◽

Vol 53 (62) ◽

pp. 8755-8758 ◽

Cited By ~ 4

Author(s):

Song Wang ◽

Fei Liu ◽

Xiang-Ling Li

Keyword(s):

Drug Delivery ◽

Drug Release ◽

Mesoporous Silica ◽

Tumor Marker ◽

Silica Nanoparticles ◽

Drug Delivery System ◽

Mesoporous Silica Nanoparticles ◽

On Demand ◽

Controllable Release ◽

Internal Stimulus

We constructed a versatile drug delivery system using dual internal stimulus, achieving controllable release and monitoring simultaneously.

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Fabrication and performance of a spherical cellulose nanocrystal-based hydrophobic drug delivery vehicle using rubber wood

BioResources ◽

10.15376/biores.14.4.7763-7774 ◽

2019 ◽

Vol 14 (4) ◽

pp. 7763-7774

Author(s):

Dingyuan Zheng ◽

Yating Deng ◽

Yue Xia ◽

Yiman Nan ◽

Meijiao Peng ◽

...

Keyword(s):

Drug Delivery ◽

Drug Release ◽

Drug Carrier ◽

Raw Material ◽

Release Mechanism ◽

Ammonium Bromide ◽

Hydrophobic Drug ◽

Rubber Wood ◽

Model Drug ◽

And Performance

Cellulose nanocrystals (CNCs) were fabricated using rubber wood (RW) as the raw material via acid hydrolysis followed by ultrasonication. The CNCs samples were then grafted with succinic anhydride to obtain modified CNCs, hereafter called CS. The CS samples were subsequently coated with a cationic surfactant, cetyltrimethyl ammonium bromide, and the obtained samples were named as CC. The morphology, chemical structure, and thermal stability of the RW, CNCs, CS, and CC samples were characterized using transmission electron microscopy, Fourier-transform infrared spectroscopy, and thermogravimetric analysis, respectively. Finally, the drug release performance was investigated using CC as the drug carrier and hesperidin, a hydrophobic drug, as the model drug. The drug release mechanism was also considered. The results of this study identified a new route for the high-value utilization of RW and also demonstrated that RW could be used as a novel substrate for the construction of cellulose-based hydrophobic drug delivery systems.

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