scholarly journals Determination of polyadenylate-rich ribonucleic acid in the nucleus and in the cytoplasm of plasmacytoma cells

1975 ◽  
Vol 149 (3) ◽  
pp. 669-674 ◽  
Author(s):  
K A Abraham ◽  
T S Eikhom
Keyword(s):  
Ph Dependence ◽  
Specific Radioactivity ◽  
Nuclear Fraction ◽  
High Concentration ◽  
Millipore Filter ◽  
Filter Technique ◽  
Short And Long Term ◽  
Millipore Filters

A number of parameters affecting the adsorption of rRNA and poly(A)-containing RNA to Millipore filters were investigated separately. Binding of both types of RNA to the filter was dependent on the concentration of RNA, pH and Mg2+ concentration of the reaction mixture. Both types of RNA bound to the filter optimally at slightly acid pH values. The binding of poly(A)-containing RNA to the filter exhibited a broad pH-dependence compared with that of rRNA. The ratio of poly(A)-rich RNA/rRNA retained by the filter was maximal between pH7 and 8. The presence of 1 mM-EDTA or a high concentration of NaCl (over 0.5M) decreased the affinity of RNA for the filter. The amount of poly(A)-containing RNA in the nucleus and in the cytoplasm of a plasmacytoma cell line (MPC-11) labelled with [32P]Pi was determined by the Millipore-filter technique under conditions that minimized contamination by rRNA. These data were compared with the estimations made by oligo(dT)-cellulose chromatography. The results obtained by these two methods were in good agreement for RNA labelled for short periods (up to 2h). In long labelling and pulse-chase experiments, however, contamination of the filter by rRNA of increasing specific radioactivity in the cytoplasm gave an erroneous value for poly(A)-containing RNA by the Millipore-filter technique. Determinations made on the nuclear fraction by these two methods did not show significant variation in short- and long-term labelling experiments.

Increase associated risk of gynaecological cancer due to long-term exposure to high concentration of atmospheric SO2 industrial pollutant

2021 ◽  
pp. 1420326X2110036
Author(s):  
Qian Xu ◽  
Chan Lu ◽  
Rachael Gakii Murithi ◽  
Lanqin Cao
Keyword(s):  
Time Windows ◽  
Air Pollutant ◽  
Gynaecological Cancer ◽  
High Concentration ◽  
The Past ◽  
Short And Long Term ◽  
Using Data ◽  
Air Quality Monitoring Stations ◽  
Control Study

A cohort case–control study was conducted in XiangYa Hospital, Changsha, China, which involved 305 patients and 399 healthy women, from June 2010 to December 2018, to evaluate the association between Chinese women’s short- and long-term exposure to industrial air pollutant, SO2 and gynaecological cancer (GC). We obtained personal and family information from the XiangYa Hospital electronic computer medical records. Using data obtained from the air quality monitoring stations in Changsha, we estimated each woman’s exposure to the industrial air pollutant, sulphur dioxide (SO2), for different time windows, including the past 1, 5, 10 and 15 years before diagnosis of the disease. A multiple logistic regression model was used to assess the association between GC and SO2 exposure. GC was significantly associated with long-term SO2 exposure, with adjusted odds ratio (95% confidence interval) = 1.56 (1.10–2.21) and 1.81 (1.07–3.06) for a per interquartile range increase in the past 10 and 15 years, respectively. Sensitivity analysis showed that different groups reacted in different ways to long-term SO2 exposure. We concluded that long-term exposure to high concentration of industrial pollutant, SO2 is associated with the development of GC. This finding has implications for the prevention and reduction of GC.

SIMPLIFIED MILLIPORE FILTER TECHNIQUE FOR CYTOLOGIC DIAGNOSIS OF CYTOMEGALIC INCLUSION DISEASE IN EXAMINATION OF URINE

PEDIATRICS ◽  
1962 ◽  
Vol 29 (1) ◽  
pp. 61-64
Author(s):  
William A. Blanc ◽  
Russel Gaetz
Keyword(s):  
Body Fluids ◽  
Screening Tests ◽  
Millipore Filter ◽  
Cytologic Diagnosis ◽  
Cytologic Examination ◽  
Filter Technique ◽  
Cytomegalic Inclusion Disease ◽  
Diagnostic Applications ◽  
Filtration Technique ◽  
Millipore Filters

A simple and practical filtration technique, with small Millipore filters, has been used for cytologic examination of urine in children. It has proved useful in routine screening tests when generalized cytomegalic inclusion disease was suspected. It could lend itself to various diagnostic applications in the cytology of urine and of other body fluids.

scholarly journals Quantitative Determination of Vinpocetine in Dietary Supplements

2016 ◽  
Vol 11 (5) ◽  
pp. 1934578X1601100
Author(s):  
John M. T. French ◽  
Matthew D. King ◽  
Owen M. McDougal
Keyword(s):  
United States ◽  
Quantitative Determination ◽  
Dietary Supplements ◽  
Nootropic Drugs ◽  
Regulatory Policies ◽  
Short And Long Term ◽  
Pharmacologically Active ◽  
Active Substances

Current United States regulatory policies allow for the addition of pharmacologically active substances in dietary supplements if derived from a botanical source. The inclusion of certain nootropic drugs, such as vinpocetine, in dietary supplements has recently come under scrutiny due to the lack of defined dosage parameters and yet unproven short- and long-term benefits and risks to human health. This study quantified the concentration of vinpocetine in several commercially available dietary supplements and found that a highly variable range of 0.6–5.1 mg/serving was present across the tested products, with most products providing no specification of vinpocetine concentrations.

scholarly journals Features of the distribution of molecular hydrogen in groundwater in various geological and tectonic conditions of Uzbekistan

2021 ◽  
Vol 929 (1) ◽  
pp. 012009
Author(s):  
R K Umurzakov ◽  
Sh S Yusupov
Keyword(s):  
Hydrogen Concentration ◽  
Molecular Hydrogen ◽  
Seismic Events ◽  
Gas Composition ◽  
High Concentration ◽  
Southwestern Part ◽  
Aeration Zone ◽  
Fluid Conductivity

Abstract As a result of generalization and analysis of previously performed work on the determination of hydrogen on the day surface, a picture of the distribution of hydrogen concentration in the aeration zone was obtained. The increased concentration of hydrogen on the day surface and in the aeration, zone is associated with the most favourable conditions for the transit of fluids from the folded Palaeozoic basement to the surface cover (upper part of the Earth’s crust). These zones are zones of active fluid conductivity, which leads to a high concentration of hydrogen on the day surface. Intense linear anomalies are confined to the zone of intersection of the Tuzkoy-Rometan transverse fault with the Bukhara-Gissar regional fault, with which seismic events are associated (epicentres Gazli earthquakes occurred 08.04.1976, 17.05.1976 and 19.03.1984). Analysis of long-term observations of gas composition on an example of molecular hydrogen in groundwater southwestern part Karzhantaus fault, covering the territory of Tashkent city and its surroundings, obtained hydrogen concentration distribution pattern area. A different concentration of hydrogen in the fault zone was revealed, associated with the heterogeneity of the geological and structural structure.

The Evaluation of the PXQ for the Analysis of Cements and Related Materials

1958 ◽  
Vol 2 ◽  
pp. 215-227
Author(s):  
George Andermann ◽  
J. L. Jones ◽  
E. Davidson
Keyword(s):  
Flow Rate ◽  
Gas Flow ◽  
Applied Research ◽  
Production Control ◽  
Helium Flow ◽  
X Ray ◽  
Long Term Stability ◽  
Short And Long Term

AbstractThe analysis of cements and ores has been studied using the Applied Research Laboratories, Inc. Production Control X-ray Quantometer (PXQ), Elements included in the program were magnesium, aluminum, silicon, phosphorus, calcium and iron. The PXQ, utilizing the polychromator concept, allows the simultaneous determination of the listed elements.Focusing ADP, EDT, quartz and LiF crystals were used with flow Geigers or Multitrons. Helium paths were used as required. The choice of crystals, detectors, and slit widths was determined to give optimum results for each element.The effects of briquetting and ratioing to scattered background on accuracy were studied. Various instrumental factors such as helium flow rate, detector gas flow rate, short and long term stability were also investigated.

Determination of native (wood derived) formaldehyde by the desiccator method in particleboards generated during panel production

Holzforschung ◽  
2010 ◽  
Vol 64 (4) ◽  
Author(s):  
Michael J. Birkeland ◽  
Linda Lorenz ◽  
James M. Wescott ◽  
Charles R. Frihart
Keyword(s):  
Hot Pressing ◽  
Urea Formaldehyde ◽  
Chromotropic Acid ◽  
Monitoring Methods ◽  
Emission Testing ◽  
Short And Long Term ◽  
The Common ◽  
Short Time

Abstract Hot-pressing wood, particularly in the production of wood composites, generates significant “native” (wood-based) formaldehyde (FA), even in the absence of adhesive. The level of native FA relates directly to the time and temperature of hot-pressing. This native FA dissipates in a relatively short time and is not part of the long-term FA emission issue commonly associated with hydrolyzing urea-formaldehyde bonds. This paper demonstrates that the common desiccator/chromotropic acid method is very specific for FA and is not influenced by other volatile compounds set free from wood during hot-pressing. Furthermore, it is shown that particleboard produces native FA at high levels even in the absence of adhesives or in the presence of one type of no-added formaldehyde (NAF) adhesive. Soy-based adhesives suppress native FA emission and provide low FA emission levels in both the short and long term. This study highlights an often overlooked aspect that should be considered for emission testing: standardizing the time and conditions employed immediately after pressing and prior to the onset of emissions testing. Addressing this issue in more detail would improve the reliability of correlation between data obtained by rapid process monitoring methods and emission measurements in large chambers.

Rubidium Sulfate Flame Detector: Effect of Design of Alkali Source and Anode on Sensitivity and Stability

1969 ◽  
Vol 52 (3) ◽  
pp. 533-541
Author(s):  
Hiroko K DeLoach ◽  
D D Hemphill
Keyword(s):  
Detection System ◽  
Physical Stability ◽  
Optimum Conditions ◽  
Flame Detector ◽  
Short And Long Term ◽  
Rubidium Sulfate ◽  
Relationship Of ◽  
The Relationship

Abstract An improved alkali-flame detector using pure rubidium sulfate and an anode of new design were optimized for the quantitative determination of organophosphate pesticides. The performance of this detection system was evaluated for response to organophosphate standards, noise level, fluctuation of baseline, short- and long-term reproducibility at low pesticides levels, conditioning time of instrument, life and physical stability of alkali source, and required frequency of detector cleaning. Under optimum conditions the detector was sensitive to 1 pg pesticide standard. To define the parameters of the alkali flame detector, studies were made of different designs of the alkali source which influence flame configuration and the relationship of the position and shape of the anode to the flame. The most important parameter appears to be the method of introducing alkali vapor.

scholarly journals Development studies of captopril certified reference material

2011 ◽  
Vol 47 (2) ◽  
pp. 339-350 ◽  
Author(s):  
Raquel Nogueira ◽  
Eliane Cristina Pires do Rego ◽  
Marcus Vinicius Barreto Sousa ◽  
Wagner Wollinger ◽  
Thaís Elias da Silva ◽  
...  
Keyword(s):  
Reference Material ◽  
Certified Reference Material ◽  
Active Pharmaceutical Ingredient ◽  
Stability Studies ◽  
Long Term Stability ◽  
Short And Long Term ◽  
Determination Of Impurities ◽  
Mass Balance Approach

This paper describes the studies performed with the candidate Certified Reference Material (CRM) of captopril, the first CRM of an active pharmaceutical ingredient (API) in Brazil, including determination of impurities (organic, inorganic and volatiles), homogeneity testing, short- and long-term stability studies, calculation of captopril content using the mass balance approach, and estimation of the associated measurement uncertainty.

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