scholarly journals Increasing levels of two different fish oils lower ruminal biohydrogenation of eicosapentaenoic and docosahexaenoic acid in vitro

2003 ◽  
Vol 52 (4) ◽  
pp. 309-320 ◽  
Author(s):  
Frigga Dohme ◽  
Veerle Fievez ◽  
Katleen Raes ◽  
Daniel I. Demeyer
Keyword(s):  
Docosahexaenoic Acid ◽  
Fish Oils ◽  
Ruminal Biohydrogenation

In vitro study of docosahexaenoic acid incorporation into phosphatidylcholine by enzymes of rat heart

1990 ◽  
Vol 93 (2) ◽  
Author(s):  
Marc Bouroudian ◽  
Gilles Nalbone ◽  
Alain Grynberg ◽  
Jeannie Leonardi ◽  
Huguette Lafont
Keyword(s):  
Docosahexaenoic Acid ◽  
Rat Heart ◽  
In Vitro Study ◽  
Vitro Study ◽  
Acid Incorporation

An in vitro study of the role of docosahexaenoic acid in human tau protein aggregation

2016 ◽  
Vol 35 (14) ◽  
pp. 3176-3181
Author(s):  
Elham Sadat Mostafavi ◽  
Mohammad Ali Nasiri Khalili ◽  
Sirus Khodadadi ◽  
Gholam Hossein Riazi
Keyword(s):  
Docosahexaenoic Acid ◽  
Protein Aggregation ◽  
Tau Protein ◽  
In Vitro Study ◽  
Vitro Study

scholarly journals A Device and Methodology for Continuous Hypothermic Perfusion of Explanted Large Mammalian Hearts, Followed by In Vitro Langendorff Reanimation: Pilot Studies

2010 ◽  
Vol 4 (2) ◽  
Author(s):  
Robin M. Brusen ◽  
Christopher D. Rolfes ◽  
Stephen A. Howard ◽  
Michael G. Bateman ◽  
Paul A. Iaizzo
Keyword(s):  
Docosahexaenoic Acid ◽  
Heart Transplantation ◽  
Cold Storage ◽  
Preservation Solution ◽  
Pilot Studies ◽  
Crystalloid Solution ◽  
Hypothermic Perfusion ◽  
Patient Morbidity ◽  
Cardioprotective Effects

The current methodologies of clinical heart transplantation limit the ischemic window to 4–6 h. Periods longer than this can induce dysfunction in the organ and can lead to increased patient morbidity and mortality. An alternative to the current methods of static cold storage (CS) is continuous hypothermic perfusion (CHP), where a hypothermic oxygenated crystalloid solution is mechanically perfused through the coronary arteries. This has been shown to preserve the function for up to 72 h, but the techniques have yet to be optimized. We have developed an apparatus and methodology for performing CHP on large mammalian hearts, followed by reanimation in our in vitro Langendorff apparatus (The Visible HeartTM). We are also investigating the utility of the cardioprotective agents docosahexaenoic acid and [D-Ala2, D-Leu5] enkephalin, both of which have shown cardioprotective effects in our laboratory, and we believe that their addition to the preservation solution can further extend the transplant window. A series of pilot studies has been performed to date, with modestly successful results. Hearts preserved with CHP seem to show better functionality than CS hearts but far worse functionality than hearts reanimated immediately after explant. We hope to use this system to optimize CHP methodology and eventually develop a system for prolonging the window for heart transplantation.

scholarly journals Effects of preconditioning and extrusion of linseed on the ruminal biohydrogenation of fatty acids. 2. In vitro and in situ studies

2006 ◽  
Vol 55 (4) ◽  
pp. 261-271 ◽  
Author(s):  
Fowad Akraim ◽  
Marie-Claude Nicot ◽  
Pierre Weill ◽  
Francis Enjalbert
Keyword(s):  
Fatty Acids ◽  
In Situ Studies ◽  
Ruminal Biohydrogenation

scholarly journals Omega-3 fatty acids and cardiovascular disease: epidemiology and effects on cardiometabolic risk factors

2014 ◽  
Vol 5 (9) ◽  
pp. 2004-2019 ◽  
Author(s):  
Trevor A. Mori
Keyword(s):  
Risk Factors ◽  
Cardiovascular Disease ◽  
Fatty Acids ◽  
Docosahexaenoic Acid ◽  
Cardiometabolic Risk ◽  
Epidemiological Studies ◽  
Fish Oils ◽  
Omega 3 Fatty Acids ◽  
Omega 3 ◽  
Disease Epidemiology

Clinical and epidemiological studies provide support that the polyunsaturated omega-3 fatty acids eicosapentaenoic acid and docosahexaenoic acid from fish and fish oils are cardioprotective, particularly in the setting of secondary prevention.

Evaluating the In Vitro Metabolism of Docosahexaenoic Acid in Sheep Rumen Fluid

Lipids ◽  
2012 ◽  
Vol 47 (8) ◽  
pp. 821-825 ◽  
Author(s):  
Noelia Aldai ◽  
Gonzalo Hervás ◽  
Álvaro Belenguer ◽  
Pilar Frutos ◽  
Angel R. Mantecón ◽  
...  
Keyword(s):  
Docosahexaenoic Acid ◽  
Rumen Fluid ◽  
In Vitro Metabolism ◽  
Sheep Rumen

scholarly journals Docosahexaenoic Acid-Loaded Polylactic Acid Core-Shell Nanofiber Membranes for Regenerative Medicine after Spinal Cord Injury: In Vitro and In Vivo Study

2020 ◽  
Vol 21 (19) ◽  
pp. 7031
Author(s):  
Zhuo-Hao Liu ◽  
Yin-Cheng Huang ◽  
Chang-Yi Kuo ◽  
Chao-Ying Kuo ◽  
Chieh-Yu Chin ◽  
...  
Keyword(s):  
Spinal Cord ◽  
Spinal Cord Injury ◽  
Docosahexaenoic Acid ◽  
Neurite Outgrowth ◽  
Polylactic Acid ◽  
In Vivo Study ◽  
Core Shell ◽  
Cord Injury

Spinal cord injury (SCI) is associated with disability and a drastic decrease in quality of life for affected individuals. Previous studies support the idea that docosahexaenoic acid (DHA)-based pharmacological approach is a promising therapeutic strategy for the management of acute SCI. We postulated that a nanostructured material for controlled delivery of DHA at the lesion site may be well suited for this purpose. Toward this end, we prepare drug-loaded fibrous mats made of core-shell nanofibers by electrospinning, which contained a polylactic acid (PLA) shell for encapsulation of DHA within the core, for delivery of DHA in situ. In vitro study confirmed sustained DHA release from PLA/DHA core-shell nanofiber membrane (CSNM) for up to 36 days, which could significantly increase neurite outgrowth from primary cortical neurons in 3 days. This is supported by the upregulation of brain-derived neurotropic factor (BDNF) and neurotrophin-3 (NT-3) neural marker genes from qRT-PCR analysis. Most importantly, the sustained release of DHA could significantly increase the neurite outgrowth length from cortical neuron cells in 7 days when co-cultured with PLA/DHA CSNM, compared with cells cultured with 3 μM DHA. From in vivo study with a SCI model created in rats, implantation of PLA/DHA CSNM could significantly improve neurological functions revealed by behavior assessment in comparison with the control (no treatment) and the PLA CSNM groups. According to histological analysis, PLA/DHA CSNM also effectively reduced neuron loss and increased serotonergic nerve sprouting. Taken together, the PLA/DHA CSNM may provide a nanostructured drug delivery system for DHA and contribute to neuroprotection and promoting neuroplasticity change following SCI.

scholarly journals A Higher Proportion of Eicosapentaenoic Acid (EPA) When Combined with Docosahexaenoic Acid (DHA) in Omega-3 Dietary Supplements Provides Higher Antioxidant Effects in Human Retinal Cells

Antioxidants ◽  
2020 ◽  
Vol 9 (9) ◽  
pp. 828 ◽  
Author(s):  
Manuel Saenz de Viteri ◽  
María Hernandez ◽  
Valentina Bilbao-Malavé ◽  
Patricia Fernandez-Robredo ◽  
Jorge González-Zamora ◽  
...  
Keyword(s):  
Docosahexaenoic Acid ◽  
Eicosapentaenoic Acid ◽  
Cell Viability ◽  
Pigment Epithelium ◽  
Retinal Pigment ◽  
Ethyl Esters ◽  
Omega 3 ◽  
Rpe Cells ◽  
Antioxidant Effects

Retinal pigment epithelium (RPE) is a key regulator of retinal function and is directly related to the transport, delivery, and metabolism of long-chain n-3 polyunsaturated fatty acids (n3-PUFA), in the retina. Due to their functions and location, RPE cells are constantly exposed to oxidative stress. Eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) have shown to have antioxidant effects by different mechanisms. For this reason, we designed an in vitro study to compare 10 formulations of DHA and EPA supplements from different origins and combined in different proportions, evaluating their effect on cell viability, cell proliferation, reactive oxygen species production, and cell migration using ARPE-19 cells. Furthermore, we assessed their ability to rescue RPE cells from the oxidative conditions seen in diabetic retinopathy. Our results showed that the different formulations of n3-PUFAs have a beneficial effect on cell viability and proliferation and are able to restore oxidative induced RPE damage. We observed that the n3-PUFA provided different results alone or combined in the same supplement. When combined, the best results were obtained in formulations that included a higher proportion of EPA than DHA. Moreover, n3-PUFA in the form of ethyl-esters had a worse performance when compared with triglycerides or phospholipid based formulations.

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