scholarly journals Optimization of core phases of biolistic transformation of potato

2020 ◽  
Vol 23 ◽  
pp. 01007
Author(s):  
Natalya P. Malakhova ◽  
Yuriy A. Skiba ◽  
Botakoz K. Tezekbayeva ◽  
Elina R. Maltseva ◽  
Gulnara A. Ismagulova
Keyword(s):  
Exposure Time ◽  
Potato Variety ◽  
Plant Material ◽  
Callus Tissue ◽  
Ms Medium ◽  
Regenerative Capacity ◽  
Before And After ◽  
Test Plants ◽  
Internode Explants ◽  
Regenerant Plants

We have established the optimal exposure time for internodes and callus plant material of Aksor potato variety on the OSS osmotic medium before and after biolistics and the type of plant explant that is most suitable for that. The optimal objects were explants from internodes of test plants. The best time for exposure of plant material to the osmotic medium before bombardment is 24 hours, and after biolistics – 48 hours. Callus tissue developed on the MS medium with zeatin two weeks later in internodes that were cultivated before bombarding on the OSS for 24 hours and we observed an increase in the callus mass by the end of fourth week. Not a single callus was obtained with rhizogenesis on the R4 medium from internodes and potato calli that were on the osmotic medium within 24 and 72 hours after biolistics. The regenerative capacity of internodes after cultivation on an osmotic medium for 48 hours after bombarding was higher than that of calli. We obtained 12 regenerant plants out of 200 internode explants and 4 regenerant plants out of 150 calli that were used in the experiment.

scholarly journals The Effect of Slow-grown in Vitro Storage Under Different Light Spectra on Banana Plantlets Cv. Prata Catarina (AAB).

2021 ◽  
Author(s):  
Paulo Hercilio Viegas Rodrigues ◽  
Emerson Oliveira ◽  
Christian Demetrio ◽  
Guilherme Ambrosano ◽  
Sônia Maria Stefano Piedade
Keyword(s):  
Plant Material ◽  
Slow Growth ◽  
Shoot Cultures ◽  
Ms Medium ◽  
In Vitro Storage ◽  
Light Spectra ◽  
Slow Growth Storage ◽  
C 50 ◽  
Commercial Micropropagation

Abstract Maintaining updated in vitro plant subcultures is essential for commercial micropropagation and tissue culture research. In unusual situations, the subcultures can be delay and the slow-growth in vitro storage technic could be applied to reduce the loss of plant material. The present study aimed to evaluate the slow-growth in vitro storage of banana plantlets (‘Prata Catarina’; group AAB) under different light spectra. Shoot cultures in MS medium without plant growth regulators were maintained under blue (B), red (R), red plus blue (R2B), and white (CW) light spectra (25°C ± 2°C; 50 µmol m -2 s -1 ) for up to 140 days. The plantlets maintained under the R, CW, and R2B spectra did not survive after 140 days of in vitro slow-growth storage. The plantlets maintained under the B spectrum survived after 140 days of in vitro slow-growth storage and showed little browning.

Abstract P537: Reduced Cardiovascular Regenerative Capacity is Associated With Enhanced Inflammatory Response to Acute Psychological Stress

Circulation ◽  
2020 ◽  
Vol 141 (Suppl_1) ◽  
Author(s):  
Zakaria Almuwaqqat ◽  
Jeong Hwan Kim ◽  
Muhammad Hammadah ◽  
Shabatun Islam ◽  
Bruno B Lima ◽  
...  
Keyword(s):  
Inflammatory Response ◽  
Vascular Function ◽  
Mental Stress ◽  
Mononuclear Cells ◽  
Patient Characteristics ◽  
Adverse Outcomes ◽  
Regenerative Capacity ◽  
Vascular Regeneration ◽  
Before And After ◽  
Artery Disease

Background: Bone marrow-derived progenitor cells (PCs) are involved in vascular regeneration and correlate with vascular function and cumulative cardiovascular risk. Systemic inflammation is associated with increased mobilization and differentiation of circulating PCs (CPCs) which may ultimately lead to exhaustion of vascular regenerative capacity. Individuals with coronary artery disease (CAD) exhibit a pro-inflammatory response to a mental stress challenge that has been associated with an elevated risk of adverse outcomes. We sought to determine whether subjects with reduced numbers of circulating PCs (CPCs) are at higher risk of a pro-inflammatory response to acute mental stress. Methods: 500 outpatients with stable CAD were enrolled into the Mental Stress Ischemia Prognosis study and underwent a laboratory-based mental stress protocol. Mononuclear cells expressing CD45med, CD34 and CXCR4 epitopes, known to be enriched for hematopoietic PCs, were enumerated using flow cytometry. Interleukin-6 (IL6) and C-Reactive Protein (CRP) levels were measured before and after mental stress. Baseline and changes in IL6 levels were compared across CPC tertiles using linear regression after adjusting for patient characteristics. Results: Mean age was 63± 9 years, 77% male, 70% white. Median CD34+ CPC count was 1.64 (1.02-2.43 cells/μL. CPC levels were not associated with either the baseline IL6 level (Beta= 0.071 95%CI, -0.091, 0.23) or CRP levels (Beta, 0.60, 95%CI, -0.25, 0.44). However, independent of demographics, CAD risk factors and baseline IL6 levels, lower CD34+/CXCR4+ CPC counts were associated with a higher inflammatory response during mental stress, measured as a rise in IL6 level (Beta= -0.11, 95%CI, -0.20, -0.028). Conclusions: Patients with reduced CPC levels have a greater pro-inflammatory response to mental stress. Thus, the observed higher risk in subjects with impaired regenerative capacity might be at least partly due to a higher stress-related pro-inflammatory response.

scholarly journals Comparative analyses of stevioside between fresh leaves and in-vitro derived callus tissue from Stevia rebaudiana Bert. using HPLC

2015 ◽  
Vol 49 (4) ◽  
pp. 199-204 ◽  
Author(s):  
S Mahmud ◽  
S Akter ◽  
IA Jahan ◽  
S Khan ◽  
A Khaleque ◽  
...  
Keyword(s):  
Retention Time ◽  
Callus Tissue ◽  
Callus Formation ◽  
Leaf Explants ◽  
Stevia Rebaudiana ◽  
Poor Performance ◽  
The Other ◽  
Ms Medium ◽  
Green Callus

A protocol was developed to produce large amount of callus in short a period of time from leaf explants of Stevia rebaudiana Bert. The highest amount of white callus was obtained on MS medium supplemented with 2.5 mg/l 2, 4-D and 0.5 mg/l BAP after 3 weeks of inoculating leaf segments. On the other hand, 0.5 mg/l BAP and 1.0 mg/l Kn exhibits poor performance towards callus formation while after using 1.0 mg/l Kn alone did not develop any callus. In this experiment, highest amount of green callus was obtained when MS medium supplemented with 2.5 mg/l NAA and 10% coconut water was used. An improved analytical method HPLC was applied to analyze stevioside extracted from the leaf and callus of Stevia rebaudiana. The stevioside in each sample were analyzed by comparing their retention times with those of the standards. The retention time (RT) of stevioside for leaves were found 14.96 and for callus 13.81 mins. The percentage of stevioside content from leaves and callus was 12.19% and 12.62% respectively DOI: http://dx.doi.org/10.3329/bjsir.v49i4.22621 Bangladesh J. Sci. Ind. Res. 49(4), 199-204, 2014

THE EFFECT OF A NEW ANTISEPTIC (l,6-DI-4′-CHLOROPHENYLDIGUANIDOHEXANE) ON SKIN FLORA

1956 ◽  
Vol 2 (2) ◽  
pp. 87-93 ◽  
Author(s):  
G. E. Myers ◽  
W. C. MacKenzie ◽  
K. A. Ward
Keyword(s):  
Aqueous Solution ◽  
Exposure Time ◽  
Bactericidal Activity ◽  
Wide Range ◽  
Skin Flora ◽  
Before And After ◽  
Measured Time ◽  
Skin Biopsies

A tincture and an aqueous solution containing 0.5% of 1,6-di-4′-chlorophenyl-diguanidohexane were tested in vivo as preoperative skin antiseptics. Skin biopsies were taken from various operative sites before and after a measured time of exposure to the antiseptic. Various periods of exposure were employed. The antiseptic was neutralized immediately at the end of the exposure time and bactericidal activity was tested by a standardized series of culture procedures. Tested under these conditions both the 0.5% aqueous solution and the 0.5% tincture possess marked skin disinfecting properties against a wide range of microorganisms.

Plant deposition in modern volcanic environments

1993 ◽  
Vol 84 (3-4) ◽  
pp. 275-281 ◽  
Author(s):  
Robyn J. Burnham
Keyword(s):  
Plant Material ◽  
Agricultural Land ◽  
Coniferous Forest ◽  
Volcanic Eruptions ◽  
Absolute Magnitude ◽  
Taxonomic Composition ◽  
Depositional Environments ◽  
Temperate Climate ◽  
Rare Elements ◽  
Before And After

ABSTRACTVolcanic eruptions and their deposits provide paleobiologists with an array of depositional environments in which to investigate the conditions in which exceptionally preserved flora and fauna are preserved. Studies of vegetation patterns before and after eruptions have shown that tropical vegetation makes a very rapid recovery at the vegetational level (proportion of devastated land covered). The recolonisation of the rare elements of a diversified flora, however, is slow, and so is the rebound of floristic richness, assembled over centuries from surrounding patches of vegetation and from in situ speciation.Two major volcanic events that occurred in the past 15 years were studied in an attempt to understand the processes and complex patterns of plant deposition in volcanic landscapes. Both volcanoes gave rise to terrestrial, andesitic, explosive eruptions that gave little warning of their absolute magnitude or duration. In both eruptions, sediment-dammed lakes were formed in which a large quantity of plant material was trapped. In both instances, forests were killed and both standing and fallen trunks record the composition of the pre-eruption flora. The vegetation present in the vicinity of the volcanoes was, in the case of Mount St Helens (Washington, U.S.A.), dense coniferous forest living in a cool temperate climate and, in the case of El Chichón (Chiapas, Mexico), remnants of paratropical rainforest alternating with patches of agricultural land.Litter layers are present under the tephra at both volcanic sites, yet the pattern of deposition and quality of the plant material differs dramatically between the two, in part because of differences in the types of eruptions and in part because of the nature of the plants available for burial. One of the most significant styles of burial, unexpected in the air-fall ash deposits, is the presence of more than one eruptive layer generated by eruptions only hours apart. These separate eruption layers have different lithological characteristics and the plant deposits buried in the different layers are different in taxonomic composition. Significantly, leaves in the upland volcanic-ash deposits are preserved thus far for 10 years, even in the tropical settings where root growth might be expected to have obliterated all signs of depositional stratigraphy. These impressions and compressions have a high likelihood of entering the fossil record and provide an excellent example of upland deposition and preservation.

scholarly journals BIOCHEMICAL AND PHYSIOLOGICAL CHANGES OF CALLUS GROWTH AND LYCOPENE PIGMENT PRODUCTION FROM TOMATO (Lycopersicon esculentum Mill.) UNDER DROUGHT STRESS

2018 ◽  
pp. 7-21
Author(s):  
Talib Khashan Kareem ◽  
Abbas Tikki Karrar
Keyword(s):  
Drought Stress ◽  
Lycopersicon Esculentum ◽  
Callus Tissue ◽  
Tomato Fruit ◽  
Sugar Content ◽  
Mother Plant ◽  
Ms Medium ◽  
Lycopersicon Esculentum Mill

This experiment was conducted in faculty of Science labs, Kufa University, carried out during 2015 to applied methods for extraction, purification and Quantitative of Lycopene red pigments, from callus tissue and tomato fruits mother plant (Lycopersicon esculentum Mill).This study include of three parts, Firstly; Tomato seeds(Supper queen) hybrid were germinated in free MS medium and callus induction from shoot tip (3cmpieces) by using MS medium supplemented with Dichlorophenoxiactic acid (2,4-D) at different concentration (0.5,1, 1.5mg/l)with benzyl adenine (BA) at concentration of (0.3 mg/l). Secondly; identically callus fresh weight re-cultured in the same MS medium supplemented with high molecular weight polyethylene glycol (PEG) was used as selective agent at level of (5,10,15 and 25%). Thirdly; comparisons study were made between in vitro and in vivo grown plant. Powder of control lycopene used as standard solution. The content of lycopene was done by using high performance liquid chromatography (HPLC), and compare of the quantitatively of lycopene with these content in fruits of mother plant, and callus tissue. Also, include alcohol extraction of Lycopene from tomato fruit by using acetone and hexane mixture. The result showed significant increased (P< 0.05) of lycopene production and the superiority of lycopene content in callus than the content in fruits of mother plant. Antioxidant enzymes activity like Catalase (CAT),Guaiacol peroxidise (POX) and Superoxide dismutase(SOD) were high in callus under drought stress than in fruit of mother plant. However, Proline and total sugar content were at higher levels in callus under drought stress than in fruit of mother plant.

scholarly journals Callus Induction and Shoot Formation for Mexican Red Bean (Phaseolus vulgaris L.) Pinto Cultivar in Vitro

2020 ◽  
pp. 1887-1893
Author(s):  
Rasha K. Mohammed Al-Saedi ◽  
Ansam G. Abdulhalem
Keyword(s):  
Growth Regulators ◽  
Culture Medium ◽  
Shoot Formation ◽  
Naphthalene Acetic Acid ◽  
Ms Medium ◽  
Phaseolus Vulgaris L ◽  
Different Types ◽  
Red Bean ◽  
Internode Explants

     The current study aimed to adopt a method for inducing callus cells and regenerating the important common red bean using different types of growth regulators such as N6-benzylaminopurine (BAP), Naphthalene acetic acid (NAA), and Thidiazuron (TDZ). Different types of common bean pinto cultivar explants, such as  internodes, cotyledons and roots,  were inoculated on Murashige and Skoog medium (MS) provided with different combinations of plant growth regulators, including 1- BAP (5 mg/l) 2-BAP (4.5 mg/l) NAA (0.5 mg/l), 3- BAP (4.5 mg/l), and TDZ (0.1mg/l). Callus was initiated on MS culture medium supplied with 5 mg/l BAP for all explants (internodes, cotyledons, and roots) at 50, 20, and 10% respectively, while adding NAA with 0.5mg/l showed a low percentage of callus (30%) only in the internode explants. Optimum results were obtained by growing the internodes on MS medium with 4.5 mg/l BA and either 0.5 mg/l NAA or 0.1 mg/l TDZ, transplanting the derived shoots into internodes and cotyledons with 70 and 10% respectively. This study concludes that the internodes as explants have the best growth results.

scholarly journals Salinity-Induced Alterations in the Growth, Membranes and Osmolytes of Musa spp. cv. Caipira (AAA) During Micropropagation

2019 ◽  
Vol 11 (6) ◽  
pp. 171
Author(s):  
Yuri Lima Melo ◽  
Isabele Aragão Gomes Trindade ◽  
Monique Cristina Simão Lopes ◽  
Cibelley Vanúcia Santana Dantas ◽  
Josemir Moura Maia ◽  
...  
Keyword(s):  
Survival Rate ◽  
Plant Height ◽  
Exposure Time ◽  
Root Length ◽  
Growth Traits ◽  
In Vitro Rooting ◽  
Ms Medium ◽  
Musa Spp ◽  
Number Of Leaves

The aim of this study was to determine the concentration and exposure time to NaCl suitable for the micropropagation of banana, through the analysis of growth traits. Banana propagules were inoculated in MS medium with different concentrations of NaCl (0; 50; 75 and 100 mM) for 120 days (multiplication and rooting, 60 days each), with monthly subcultures. These propagules were measured for plant height, number of leaves, sprouting rate, average number of formed propagules, rooting rate, root length and survival rate. After 30 days, NaCl reduced sprouting rate at multiplication; the number of leaves, rooting rate and root length in rooting; and the height and propagules number in both phases. After 60 days, the NaCl affected the sprouting rate and propagules number in the multiplication; length of root in rooting; and the height and number of leaves in both phases. After 120 days, the reduction in the survival rate was proportional to the increase of NaCl in the medium. Thus, it is concluded that NaCl reduces most of the growth traits and the treatments with 75 and 100 mM NaCl affected multiplication and in vitro rooting more intensely.

scholarly journals Harvest Maturity and Concentration and Exposure Time to Acetylene Influence Initiation of Ripening in Mangos

1990 ◽  
Vol 115 (3) ◽  
pp. 426-430 ◽  
Author(s):  
A.P. Medlicott ◽  
Mayé N'Diaye ◽  
J.M.M. Sigrist
Keyword(s):  
Exposure Time ◽  
Mangifera Indica ◽  
Soluble Solids ◽  
Similar Response ◽  
Immature Fruit ◽  
Color Development ◽  
Solids Concentration ◽  
Peel Color ◽  
Before And After ◽  
Harvest Maturity

The effects of acetylene at 0, 0.1, 0.2, 0.4, 0.8, or 1.6 ml·liter-1 and exposures of 4, 8, 12, or 24 hours on ripening initiation In mangos (Mangifera indica L.) harvested at three stages of maturity were investigated: Ripeness was assessed before and after treatment in `Tommy Atkins', `Ruby', and `Amelie' mangos by analysis of texture, peel, and pulp color development, soluble solids concentration, and pH. The initiation of ripening depended on the acetylene concentration, exposure time to acetylene, the physiological maturity of the fruit at harvest, and on the cultivar. Changes that can occur during ripening bad different sensitivities to acetylene gas. Acetylene treatment of 0.1 or 0.2 ml·liter-1 for 24 hours at 25C initiated softening, but had no effect on the other ripening processes measured. All the ripening changes measured were initiated with a 24-hour exposure to 0.4 ml·liter-1 in `Tommy Atkins', while 0.8 ml·liter-l was required with `Ruby' mangos. There was an interaction between gas concentration and exposure time taken to Initiate ripening. The 0.8 ml·liter-1 acetylene treatment required 24 hours to initiate full ripening, while 8 hours were required with 1.6 ml·liter-l acetylene and 1.0 ml·liter-1 ethylene. Mature and half-mature fruit showed a similar response to gas treatments; immature fruit failed to show full ripening initiation, although softening and peel color development were enhanced.

scholarly journals Phytoremediation of emulsion paint wastewater using Azolla Pinnata, Eichhornia Crassipes and Lemna Minor

2021 ◽  
Vol 40 (3) ◽  
pp. 550-557
Author(s):  
E.A. Echiegu ◽  
C.O. Ezimah ◽  
M.E. Okechukwu ◽  
O.A. Nwoke
Keyword(s):  
Eichhornia Crassipes ◽  
Lemna Minor ◽  
Physicochemical Characteristics ◽  
Treated Wastewater ◽  
Preliminary Treatment ◽  
Azolla Pinnata ◽  
Heavy Metal Concentrations ◽  
Emulsion Paint ◽  
Before And After ◽  
Test Plants

The use of three macrophytes namely Azolla pinnata, Eichhornia crassipes and Lemna minor for the phytoremediation of emulsion paint wastewater was investigated. Samples of the paint wastewater and test plants were collected and analyzed for physicochemical characteristics and heavy metal concentrations before and after phytoremediation for six weeks. The TDS of the treated wastewater was reduced by over 80.0% by each of the test plants while the TSS increased as a result of debris from withered test plants. Dissolved oxygen reduction ranged from 12.5% to 50.0%, COD from 49.5% to 57.1%, BOD from 46.7% to 54.7, heavy metals from 11.0 to 92.5%. A. pinnata appears to have performed significantly better (P < 0.05) than the other plants followed by E. crassipies and L. minor. It can be concluded that the test plants (especially A. pinnata) can be effectively used for the preliminary treatment of paint wastewater.

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