scholarly journals Influence of different concentrations of 6-benzylaminopurine and thidiazuron on the proliferative activity of apple varieties in in vitro culture

2021 ◽  
Vol 36 ◽  
pp. 03012
Author(s):  
L.V. Tashmatova ◽  
O.V. Matsneva ◽  
T.M. Khromova ◽  
V.V. Shakhov
Keyword(s):  
In Vitro Culture ◽  
Apical Dominance ◽  
Proliferative Activity ◽  
Essential Role ◽  
Cytokinin Activity ◽  
Multiplication Factor ◽  
Apple Varieties ◽  
Clonal Micropropagation ◽  
Study Results

An essential role in the process of clonal micropropagation is played by cytokinins, with the help of which apical dominance is removed and the formation of additional shoots and buds is activated. The article presents the study results of microshoots’ proliferative activity of apple varieties on media with 6-benzylaminopurine at a concentration of 1.0 mg/l, 2.0 mg/l and thidiazuron at a concentration of 0.1 mg/l, 0.2 mg/l and 0.3 mg/l. The objects of research were apple varieties of the VNIISPK selection - Bolotovskoe, Imrus, Veteran, Kandil orlovsky, Girlyanda, Priokskoe. The aim of the research was to study the effect of substances with cytokinin activity on the morphogenesis of apple varieties. As a result of the research, it was revealed that both cytokinins and varietal characteristics influence the multiplication factor. The highest multiplication factor was observed when using 6-BAP. Thidiazuron reduced the proliferation rate. When using 6-BAP, Bolotovskoe, Girlyanda and Priokskoe varieties had the highest multiplication factor among the varieties. When using TDZ - varieties Bolotovskoe and Kandil Orlovsky.

scholarly journals Clonal micropropagation of bog cranberry (Vaccinium oxycoccos L.) in the Udmurt Republic

2021 ◽  
Vol 22 (1) ◽  
pp. 57-66
Author(s):  
E. N. Cheremnykh ◽  
T. G. Lekontseva ◽  
A. V. Khudyakova ◽  
A. V. Fedorov
Keyword(s):  
In Vitro Culture ◽  
Nutrient Medium ◽  
Root Formation ◽  
Sphagnum Moss ◽  
Multiplication Factor ◽  
Clonal Micropropagation ◽  
Planting Material ◽  
Udmurt Republic

The paper presents the results of 2018-2019 research on improving the technology of growing planting material of bog cranberry (Vaccinium oxycoccos L.) of Krasa Severa, Severyanka, Virussaare varieties on the basis of in vitro. Studied was the effect of the concentrations of growth regulators in the composition of the nutrient medium according to Anderson's recipe on the reproduction and subsequent rooting of micro cuttings, as well as the duration of cultivation and adaptation of micro plants depending on partial pruning of shoots. It has been established that at the stage of introduction into in vitro culture, sterilization of explants with 33% hydrogen peroxide in an exposure of 5-8 minutes with washing in 5 portions of sterile distillate gives 60-80 % of viable shoots. The optimum phase of plant development for the successful introduction of in vitro culture is the swelling of buds. Cultivation of micro cuttings was carried out in a light room at a temperature of 25±2 °С, a photoperiod of 16 hours. The duration of each subculturing was 30-60 days. For the stage of actual micropropagation on Anderson's nutrient medium, an increase in the dose of cytokinin 6-benzylaminopurine (6-BAP) from 0.2 to 0.5 mg/l and an increase in the duration of cultivation from 30 to 60 days contributed to a significant increase in the multiplication factor on average for the tested cranberry varieties.According to the efficiency of micropropagation, the varieties Virussaare and Krasa Severa were distinguished – 9.3-12.0 pcs/stalk, respectively. At the rooting stage, the use of a root-forming reagent of indolyl-3-acetic acid (IUK) in doses of 0.2, 0.5 and 1.0 mg/l in the composition of Anderson's nutrient medium did not affect the quality of root formation and the length of shoots of Virussaare micro-plants. No significant varietal differences in the root-forming ability of microcuttings were found. The tendency of better rooting of micro cuttings was observed in the Virussaare variety (90.3 %) compared to the Severyanka (85.7 %) and Krasa Severa (79.3 %) varieties. Micro plants of the Krasa Severa cultivar were characterized by the longest shoots, the total number of roots was less, but their length was longer in comparison with other cultivars. For the adaptation stage, a substrate from a mixture of lowland peat and sphagnum moss was used (1:1). The efficiency of adaptation of micro plants of cranberry varieties when cutting the tip of the shoots was 100 %. Pruning of micro plants shoots contributed to the formation of more side shoots and better development of the aboveground part of the plants.

REGENERATION OF APPLE VARIETIES IN VITRO CULTURE

2020 ◽  
pp. 22-25
Author(s):  
L. V. Tashmatova ◽  
O. V. Matsneva ◽  
T. М. Khromova ◽  
V. V. Shakhov
Keyword(s):  
Growth Phase ◽  
Proliferative Activity ◽  
Small Degree ◽  
Nutrient Media ◽  
Regenerative Capacity ◽  
Active Growth ◽  
Apple Varieties ◽  
Clonal Micropropagation ◽  
Genotypic Characteristics

The process of clonal micropropagation is influenced by the genotypic characteristics of plants, the composition of nutrient media and the hormonal background at various stages of cultivation. The purpose of the research was to study the regenerative capacity of apple explants of promising varieties. The objects were colon-shaped, immune and scab-resistant apple varieties. The study was carried out in the laboratory of biotechnology of VNIISPK using generally accepted methods. The largest number of viable explants was obtained during the active growth phase, averaging from 72.1 to 88.5%. Maximum contamination was observed in explants introduced in April, regardless of the sterilizing agents. The highest infection rate was in the varieties Orlovskoe рolesie (up to 56.7%), Bolotovskoe (up to 51.4%), Poeziya (up to 43.3%). In the explants introduced in June, the infection was insignificant. Thus, the Imrus and Poeziya varieties showed a small degree of infection after using 0.1% mertiolate solution (1.2% and 2.4%), and the garland and Priokskoe varieties had a 100% yield of pure apexes. Among immune varieties, the highest necrosis was evident in the variety of Imrus (42,1%) and the smallest in the variety Kandil оrlovsky (5,1%) have the largest column-the variety Priokskoe (65.6 per cent) and Vostorg (65,6%). In most of the studied varieties, the greatest necrosis was observed after using 0.1% mertiolate solution. The study was carried out on the background medium QL and Fardzinova recommended for pears. Concentration of BAP 1.0 and 2.0 mg/l. Among the immune varieties, the content of BAP 2.0 mg / l in the QL medium caused the greatest proliferative activity in the varieties Kandil оrlovsky (3.4), Bolotovskoe (4.5) and Orlovskoe рolesie (2.9). Varieties of Imrus and a Veteran of the highest rate of multiplication were obtained in Fardzinova on medium containing BAP in both concentrations. In all varieties, the concentration of BAP 1.0 mg/l contributed to the formation of shoots more than 1 cm, i.e. suitable for rooting.

Research of carbohydrate type influence on Lonicera caerulea l. Morphogenetic potential in vitro

2020 ◽  
Vol 61 ◽  
pp. 54-60
Author(s):  
N. D. Orlova ◽  
D. A. Egorova
Keyword(s):  
Multiplication Factor ◽  
Lonicera Caerulea ◽  
Genetic Characteristics ◽  
Clonal Micropropagation ◽  
Morphogenetic Potential

The clonal micropropagation technique of valuable Lonicera caerulea L. varieties (Indigo Gem, Moskovskaya 23, Diana, Jugana) has been improved. The effect of various types and concentrations of carbohydrates on microshoots regeneration of the genus Lonicera L. representatives was studied. It is shown that the concentration of carbohydrates has the greatest effect on microshoots height and the type of carbohydrate nutrition has the greatest effect on multiplication factor. The type of carbohydrate nutrition (42 %) had the greatest influence on multiplication factor. Peculiarities of varieties, genetic characteristics interaction, carbohydrate nutrition type and concentration had a lesser effect (20 %, 14 % and 11 % respectively). The variety Moskovskaya 23 was characterized by the highest microshoots number (49.6 mm). The highest multiplication factor in the studied varieties, regardless of the type of carbohydrate nutrition, was observed at concentration of 40 g/l (11.57).

scholarly journals FLAVONOIDS OF IRIS SIBIRICA L. GROWN IN VITRO CULTURE

2021 ◽  
pp. 301-308
Author(s):  
Olesya Nikolayevna Mazko ◽  
Lyudmila Ivanovna Tikhomirova ◽  
Lyudmila Vladimirovna Shcherbakova ◽  
Natal'ya Grigor'yevna Bazarnova ◽  
Dmitriy Alekseyevich Karpitsky
Keyword(s):  
In Vitro Culture ◽  
High Performance ◽  
Raw Materials ◽  
Quantitative Composition ◽  
Qualitative Composition ◽  
Nutrient Media ◽  
Qualitative And Quantitative ◽  
Clonal Micropropagation ◽  
Intact Plants

The aim of this study was to evaluate the effect of 6-benzylaminopurine (BAP) separately and in interaction with auxins on the change in the qualitative and quantitative composition of flavonoids in the raw materials of regenerating plants Iris sibirica L. Cambridge grade in comparison with aeroponic and intact raw materials using the method of high-performance liquid chromatography. Raw materials of I. sibirica Cambridge variety obtained in vitro culture had a richer qualitative composition of flavonoids than intact plants. The dependence of the accumulation of flavonoids on the concentration of 6-benzylaminopurine in nutrient media was noted. The presence of 13 compounds was observed in extracts of 70% ethyl alcohol from regenerating plants grown at the lowest concentration of BAP (1.0 µM) within the experiment. In quantitative terms, the flavonoid apigenin was maximally determined on a medium with BAP 1 µM, and kaempferol - on media with BAP 5.0 µM, supplemented with auxins. For a medium with 7.5 µM BAP, the lowest variety of compounds was observed (9) and the lowest kaempferol content. Auxins influenced the synthesis of flavonoids. The amount of flavonoids in all variants of the experiment increased by an average of 13% in the presence of auxins. The stages of the technological process of obtaining raw materials I. sibirica Cambridge variety on the basis of clonal micropropagation and cultivation in aeroponics conditions allowed to obtain raw materials that do not contain heavy and toxic metals, are not infected with pathogens and pests. With 1 m2 of useful area of aeroponics for 1 year, it is possible to collect 5 times more raw materials than with field cultivation. According to the qualitative composition of phenolic compounds, aeroponic raw materials are identical to intact plants.

scholarly journals Regeneration capacity of Cerasus fruticosa and Prunus domestica into the in vitro culture

2021 ◽  
Vol 209 (06) ◽  
pp. 43-52
Author(s):  
Marina Markova ◽  
Elena Somova
Keyword(s):  
In Vitro Culture ◽  
Nutrient Medium ◽  
Optimal Time ◽  
In Vitro Cultivation ◽  
Initiation Time ◽  
Multiplication Factor ◽  
Optimal Conditions ◽  
Prunus Domestica ◽  
Nutrient Media

Abstract. The aim of these studies was to introduce into the in vitro culture the steppe cherry (Cerasus fruticosa) variety Shchedraya and the domestic plum (Prunus domestica) variety Sineokaya for subsequent micropropagation. Methods. Optimal conditions for obtaining viable explants, such as sterilizing agent and initiation time, have been investigated. The suitability of various nutrient media for in vitro cultivation of these cultures has also been tested. As a result of the experiments, it was revealed that the most effective sterilizing agents were 38 % perhydrol (control) and 6% chlorhexidine: the yield of viable cherry explants was 63.8 % and 61.5 %, plums – 69.8 % and 66.6 %, respectively. The optimal time for the initiation of cherry explants in vitro was January, where the yield of viable explants averaged 53.9 %, in June – 49.1 %, and for plums the initiation time did not matter – the yield of explants was 55.8 % in winter and 53.1 % in summer. In vitro cultivation of cherries and plums on the Quoirin – Lepoivre nutrient medium provided a significantly high multiplication factor, which averaged 4.1 for cherries (2.7 in control) and 6.0 for plums (3.9 in control). On the same medium, the maximum multiplication factor was obtained, which was 6.2 for cherries and 8.2 for plums. Thus, the scientific novelty of these studies is that the optimal conditions (sterilizing agent, time, nutrient medium) have been selected for the regeneration of cherry and plum explants in vitro with their subsequent micropropagation.

scholarly journals INFLUENCE OF THE STERILIZATION METHOD AND THE TIME OF INTRODUCTION INTO IN VITRO CULTURE ON THE VIABILITY OF EXPLANTS OF THE CLONAL APPLE STOCK 54-118

2020 ◽  
Vol 30 (4) ◽  
pp. 411-416
Author(s):  
A.V. Nikitina ◽  
A.M. Lentochkin ◽  
T.G. Lekontseva ◽  
A.V. Fedorov
Keyword(s):  
Hydrogen Peroxide ◽  
In Vitro Culture ◽  
Ethyl Alcohol ◽  
Shoot Growth ◽  
Sterile Culture ◽  
Sterilization Method ◽  
Clonal Micropropagation

The stage of introducing explants into a sterile culture is difficult in the technology of clonal micropropagation of plants. The article shows the possible ways of sterilization and the introduction terms of explants of the clonal apple stocks 54-118 into in vitro culture in order to reduce planting infection and increase the yield of sterile viable explants. The best time for introducing clonal apple stocks 54-118 into sterile in vitro culture is the period of active shoot growth. Sterilization of explants with ethyl alcohol (70,0 %, 1 min) in combination with hydrogen peroxide (33,0 %) for 7 minutes and ethyl alcohol (70,0 %, 1 min) in combination with diacide (0,1 %) within 6 minutes contributed to the production of 63,0 % and 60,0 % of viable sterile explants.

scholarly journals The influence of clonal micropropagation on productivity and differentiation of Mentha pіperіta plant tissues

2019 ◽  
Vol 10 (3) ◽  
pp. 337-344 ◽  
Author(s):  
T. E. Talankova-Sereda ◽  
J. V. Kolomiets ◽  
A. V. Holubenko ◽  
N. V. Nuzhyna
Keyword(s):  
Pharmaceutical Industry ◽  
Essential Oil ◽  
In Vitro Culture ◽  
Biomass Accumulation ◽  
System Structure ◽  
Clonal Micropropagation ◽  
Statistical Analysis Method ◽  
Productivity Indices

Peppermint grass, as a raw medicinal plant material, has great importance for the pharmaceutical industry. The influence of clonal micropropagation and chemotherapy has been established in vitro on six breeds of Ukrainian selection peppermint plants, in particular on the sprouts’ conductive system structure and tissue development, general biomass accumulation, and in vivo productivity of breeds. The influence of clonal micropropagation and chemotherapy on important productivity indices of the plants has been established in vitro in six breeds of Ukrainian selection peppermint plants as pharmacopeial plants. The linear meter method, the microscopic method, the standard histochemical methods, and the statistical analysis method were used in the studies. A clear tendency to increase in the leaf cover, air-dry leafage and rhizome was observed in breeds of Ukrainian selection peppermint to which propagation and in vitro improvement technology was applied. The air-dry leafage yield significantly increased after in vitro culture from 7.6% in the Lidiia breed to 51.4% in the Chornolysta breed recognized as a state mint standard in Ukraine. The leaf cover increased from 8% to 21% in peppermint plants improved in іn vitro culture. This method promoted essential oil quantity increase from 9.8 to 28.6 kg per hectare. The rhizome yield increased by 6.3–40.4% in all peppermint plants breeds after improvement in in vitro culture on average within one vegetation year. The Lebedyna Pisnіa and Mama breeds were characterised by the most intensive development of all investigated anatomic and morphological indices after in vitro culture: rhizomes yield increased by 40.4% and 40.1%, air-dry leafage by 37.1% and 26.6%, leaf cover by 21.0% and 13.0%, and essential oil quantity per hectare increased by 38.1% and 28.5% accordingly. Anatomical and histochemical studies of sprouts of Ukrainian selection peppermint plants breeds confirmed increase in xylogenesis intensity in the majority of the studied breeds (except Lidiia and Ukrains’ka Pertseva) after in vitro culture improvement. The xylogenesis process was most expressed in the Mama and Chornolysta breeds. Air-dry leafage, rhizome yield, and leaf cover increased in all peppermint plants breeds after in vitro improvement, which could be critical for the pharmaceutical industry.

scholarly journals Optimization of clonal micropropagation of Chrysanthemum

2019 ◽  
Vol 9 (4) ◽  
pp. 676-678
Author(s):  
I. D. Borodulina ◽  
A. Trufanova ◽  
G. Shevchenko ◽  
G. Sokolova ◽  
T. Plaksina
Keyword(s):  
Growth Regulators ◽  
Traditional Method ◽  
Proliferative Activity ◽  
Culture Medium ◽  
Ms Medium ◽  
Mineral Salts ◽  
Clonal Micropropagation ◽  
Murashige Skoog ◽  
High Proliferative Activity

Micropropagation of Chrysanthemum is an alternative to the traditional method of reproduction. Thanks to this method, the Chrysanthemum reproduction time is reduced to 3-4 months. For clonal micropropagation, sterile microshoots of Chrysanthemums of the varieties Snow White, Stranger, and Baltica White were used. At the stage of the micropropagation, the Murashige-Skoog (MS) medium with the full and half composition of mineral salts and growth regulators (kinetin, 6-benzylaminopurine, β-indolylacetic acid) were used. A universal culture medium for clonal micropropagation of all varieties of Chrysanthemum and optimal mediums, taking into account variety-specificity were established. It was noted that under in vitro conditions, high proliferative activity was observed in Neznakomka variety.

Features of clonal micropropagation of Silene cretacea (Caryophyllaceae) in in vitro culture

2016 ◽  
Vol 47 (6) ◽  
pp. 359-366 ◽  
Author(s):  
T. A. Kritskaya ◽  
A. S. Kashin ◽  
V. A. Spivak ◽  
V. E. Firstov
Keyword(s):  
In Vitro Culture ◽  
Clonal Micropropagation

scholarly journals Investigation of Cytotoxic Effects of Recombinant Human Interferon Lambda-1 and Its Pegylated Form on Human Conjunctival Epithelial Cells

2021 ◽  
Vol 9 (4) ◽  
pp. 200-208
Author(s):  
N. A. Kikhtenko ◽  
N. A. Bondarenko ◽  
N. P. Bgatova ◽  
L. A. Oleynik ◽  
O. V. Poveshchenko ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Proliferative Activity ◽  
Cytotoxic Effect ◽  
Ultrastructural Changes ◽  
Human Interferon ◽  
Interferon Lambda ◽  
Study Results ◽  
Cell Proliferative Activity ◽  
Pegylated Form

Currently, there are no efficacious, all-purpose antiviral medicines for the treatment of ocular surface infections caused by viruses. At the same time, type III interferons demonstrate high potency for histological barriers, such as the conjunctiva. Modification of protein molecules in native products can significantly improve their pharmacodynamic properties. Thus, it seems reasonable to develop antiviral medicines based on interferon lambda (IFN-λ1) and its pegylated form (PEG IFN-λ1).The aim of the study was to evaluate the in vitro cytotoxic effect of recombinant human IFN-λ1 and its pegylated form on Chang conjunctiva clone 1-5c-4 human conjunctival cells.Materials and methods: PEG IFN-λ1 was obtained by the electron beam immobilisation method. A normal human conjunctival cell line Chang conjunctiva clone 1-5c-4 was used for cell cultivation. The MTT test was used to assess the cytotoxic effect. Cell proliferative activity was studied by measuring microelectrode impedance. Ultrastructural changes were assessed by electron microscopy. Statistical processing was performed using the Statistica 10.0 software package.Results: IFN-λ1 (37 μg/mL) and PEG IFN-λ1 (42 μg/mL) had no significant cytotoxic effect on the human conjunctiva cell culture and the cell proliferative activity. The analysis of ultrastructural changes demonstrated that IFN-λ1 activated metabolic processes in the cells, and PEG IFN-λ1 promoted differentiation and keratinisation of epithelial cells and led to modification of the cell membrane. A ten-fold increase in IFN-λ1 and PEG IFN-λ1 concentration (to 370 μg/mL and 420 μg/mL, respectively) reduced the cell viability by 15–20% as compared to the intact control.Conclusions: the study results demonstrated that IFN-λ1 and PEG IFN-λ1 could be used as active pharmaceutical ingredients in the development of medicines for the treatment of conjunctival viral infections.

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