scholarly journals Influence of bacterial metabolites from permafrost on morphophysiological parameters of potato material in vitro*

2021 ◽  
Vol 36 ◽  
pp. 05009
Author(s):  
N.O. Renev ◽  
E.S. Rodina ◽  
A.M. Subbotin ◽  
V.A. Malchevskiy
Keyword(s):  
Secondary Metabolites ◽  
Bacillus Cereus ◽  
Potato Variety ◽  
Bacterial Strain ◽  
Inhibitory Effect ◽  
Potato Seed ◽  
Bacterial Strains ◽  
Plant Cultivation ◽  
Murashige Skoog

The effect of secondary metabolites of bacteria from permafrost rocks on the in vitro morphophysiological parameters of potato microplants has been studied. Microplants of Zhukovsky ranniy potato variety were used as the object of the study. The experimental results indicate that when potato microplants are grown in vitro together in the Murashige-Skoog nutrient medium with the bacteria metabolites of the Bacillus cereus 9-08-CH9 and Achromobacter spanius 10-50TS2 strains introduced at the grafting time at a dose of 250 μl, they have the highest inhibitory effect. Secondary metabolites of these bacterial strains have a significant increase in the number of internodes at all stages of plant cultivation, which can accelerate the in vitro replication of material for original potato seed production. Metabolites of the bacterial strain Bacillus cereus 875TS in the indicated concentrations cause growth inhibition and delay in the development of microplants’ root system at the initial cultivation stages.

Preliminary in Vitro Investigations on The Inhibitory Activity of The Original Dietary Supplement Oxidal® on Pathogenic Bacterial Strains

2020 ◽  
Vol 11 ◽  
pp. 37-43
Author(s):  
Prof. Teodora P. Popova ◽  
Toshka Petrova ◽  
Ignat Ignatov ◽  
Stoil Karadzhov
Keyword(s):  
Dietary Supplement ◽  
Broad Spectrum ◽  
Pathogenic Bacteria ◽  
Antimicrobial Agents ◽  
Clinical Effectiveness ◽  
Inhibitory Effect ◽  
Diffusion Method ◽  
Bacterial Strains ◽  
Microbial Strains

The antimicrobial action of the dietary supplement Oxidal® was tested using the classic Bauer and Kirby agar-gel diffusion method. Clinical and reference strains of Staphylococcus aureus and Escherichia coli were used in the studies. The tested dietary supplement showed a well-pronounced inhibitory effect against the microbial strains commensurable with that of the broad-spectrum chemotherapeutic agent Enrofloxacin and showed even higher activity than the broad spectrum antibiotic Thiamphenicol. The proven inhibitory effect of the tested dietary supplement against the examined pathogenic bacteria is in accordance with the established clinical effectiveness standards for antimicrobial agents.

scholarly journals The in vitro antimicrobial and antioxidant activity of leaves of medicinal plants with phytobiotic potential in animal production

2021 ◽  
Author(s):  
Roisbel Aroche ◽  
Xianren Jiang ◽  
Yordan Martínez ◽  
Román Rodríguez ◽  
Xilong Li ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Medicinal Plants ◽  
Secondary Metabolites ◽  
Moringa Oleifera ◽  
Psidium Guajava ◽  
Anacardium Occidentale ◽  
Morinda Citrifolia ◽  
Bacterial Strains ◽  
Antimicrobial And Antioxidant Activity

Abstract Little is known about which secondary metabolites are responsible for inhibiting pathogenic bacteria and reducing the pro-oxidant effect on the leaves of four medicinal plants used as phytobiotic in animal production. The aim of this study was to evaluate the antimicrobial and antioxidant activity of four medicinal plants (Anacardium occidentale, Psidium guajava, Morinda citrifolia and Moringa oleifera.) in vitro. A total of six bacterial strains were inoculated, then minimum bactericidal concentration (MBC) was evaluated in fine powder and minimum inhibitory concentration (MIC) and MBC were determined on the aqueous extract. Also, the in vitro antioxidant activity was evaluated through 1,1-diphenyl-2-picryl-hydrazyl, as well as the main secondary metabolites were identified and quantified by chromatographic analysis. The results showed that Anacardium occidentale and Psidium guajava leaves had higher antimicrobial activity against all bacterial strains. In addition, Morinda citrifolia inhibited S. aureus in the aqueous extract, although without in vitro bactericidal effect, while Moringa oleifera leaf did not show antimicrobial effect. All plants showed antioxidant capacity, standing out Anacardium occidentale and Psidium guajava. Mainly the leaves of Anacardium occidentale showed high concentrations of quercetin 3-O-glucoside-7-O-rhamnoside, kaempeferol-7-O-glucoside, quercetin, caffeic acid, and cinnamic acid. Apparently, the antimicrobial and antioxidant activity are due to the main polyphenolic compounds identified in medicinal plants (mainly Anacardium occidentale and Psidium guajava); however, further studies are necessary to elucidate the exact mechanism.

Rapid and efficient synthesis of newer heterocyclic 2-azetidinone and 5-benzylidine-4-oxo-thiazolidine compounds and their pharmacological studies

2019 ◽  
Author(s):  
Chem Int
Keyword(s):  
Inhibitory Effect ◽  
Diffusion Method ◽  
Spectroscopic Techniques ◽  
Antimicrobial Compounds ◽  
Efficient Synthesis ◽  
Proteus Vulgaris ◽  
Bacterial Strains ◽  
Pharmacological Studies ◽  
In Vitro Antibacterial Activity

A straightforward rapid and efficient protocol for the synthesis of 2-azetidinone (D1-10) and 5-benzylidine-4-oxo-thiazolidine (F1-10) has been designed and synthesized in order to find newer antimicrobial compounds. The structure of entitle compounds have been evaluated on the basis of various spectroscopic techniques FTIR, 1H-NMR, 13C-NMR as well as elemental microanalysis. The title compounds were screened for their preliminary in vitro antibacterial activity against a panel of selected pathogenic bacterial strains, Staphylococcus aureus (MTCC 96), Escherichia coli (MTCC 443), Proteus vulgaris (MTCC 426) and Pseudomonas aeruginosa (MTCC 424) using cup-plate agar diffusion method at 40 μg/ml concentration. Out of synthesized compounds, compound nos. D4, D5, D7, D8, D9 and D10 have shown outstanding inhibitory effect against all pathogens and consider as the best bioactive desired antibacterial analogue of the series as compare to standard drugs ampicilline and chloramphenicol.

Combined effect of Bacillus cereus CPOU13 and B. subtilis SPC14 on polycyclic aromatic hydrocarbons degradation in vitro

2016 ◽  
Vol 5 (04) ◽  
pp. 4505
Author(s):  
Poornachander Rao M.* ◽  
Anitha Y. ◽  
Satyaprasad K.
Keyword(s):  
Polycyclic Aromatic Hydrocarbons ◽  
Bacillus Cereus ◽  
Aromatic Hydrocarbons ◽  
Combined Effect ◽  
In Vitro Experiments ◽  
Bacterial Strains ◽  
Hplc Peak ◽  
Polycyclic Aromatic

Biodegradation of polycyclic aromatic hydrocarbons (PAHs) with suitable bacterial strains conveys much interest in recent years. We studied biodegradation of PAHs namely phenanthrene, anthracene and pyrene using two efficient PAHs degrading strains, B. cereus CPOU13 and B. subtilis SPC14 in vitro experiments. Standard HPLC chromatograms for phenanthrene, anthracene and pyrene were plotted separately based on HPLC peak area values and Retension time of known concentrations of the test PAHs and using software, ‘Origin 6.0’. Biodegradation of PAHs mixture containing phenanthrene, anthracene and pyrene was studied in for 13 days. We found that the combination of bacterial strains, B. cereus CPOU13 and B. subtilis SPC14 degraded high amounts of phenanthrene, anthracene and pyrene in 13 days of incubation. The recorded degradation percentages of phenanthrene, anthracene and pyrene were 85.31, 92.82 and 85.70 respectively. Concentration of phenanthrene was degraded from 217µg/5ml to 31.9µg/5ml. Concentration of anthracene was degraded from 211µg/5ml to 16µg/5ml. Concentration of pyrene was degraded from 233µg/5ml to 33µg/5ml in 13 days of incubation. We also observed biodegradation of phenanthrene, anthracene and pyrene from 1st day to 13th day.

scholarly journals In vitro antibacterial activity of Terminalia chebula leaf gall extracts against some human pathogenic strains

2012 ◽  
Vol 1 (8) ◽  
pp. 217-220 ◽  
Author(s):  
BE Ravi Shankara ◽  
YL Ramachandra ◽  
S Sundara Rajan ◽  
J Preetham ◽  
PS Sujan Ganapathy
Keyword(s):  
Antibacterial Activity ◽  
Aqueous Extract ◽  
Ethanol Extract ◽  
Pharmaceutical Journal ◽  
Inhibitory Effect ◽  
Diffusion Method ◽  
Terminalia Chebula ◽  
Bacterial Strains ◽  
South Indian

The leaf galls of Terminalia chebula is used widely as Karkatasringi in south Indian markets. Karkatashringi is an important crude drug employed in various indigenous systems of medicine against several diseases and the drug has diverse medicinal properties. The present study was carried out to understand the antimicrobial activity of various extracts. The antibacterial activity of T. chebula (leaf gall) was evaluated against ten bacterial strains including Gram-positive and Gram-negative bacteria using the agar-well diffusion method. Among the two extracts tested, the ethanol extract presented the best results against all the bacteria while aqueous extract showed moderate inhibition of the microbial growth. Each extract is unique against different microorganisms; Staphylococcus aureus was more susceptible to both extract among the tested organisms, whereas Serratia marcescens and Proteus mirabilis were less susceptible for ethanol and aqueous extract respectively. The inhibitory effect of the extracts was compared with standard antibiotic Ciprofloxacin.DOI: http://dx.doi.org/10.3329/icpj.v1i8.11254 International Current Pharmaceutical Journal 2012, 1(8): 217-220 

Secondary metabolites of Helichrysum plicatum DC. subsp. plicatum flowers as strong carbonic anhydrase, cholinesterase and α-glycosidase inhibitors

2020 ◽  
Vol 75 (5-6) ◽  
pp. 153-159
Author(s):  
Tuba Aydin
Keyword(s):  
Secondary Metabolites ◽  
Carbonic Anhydrase ◽  
Folk Medicine ◽  
Inhibitory Effect ◽  
Glycosidase Inhibitors ◽  
Meoh Extract ◽  
Chemical Structures ◽  
Liquid Chromatography Tandem Mass ◽  
First Time

AbstractHelichrysum plicatum species are used in Turkish folk medicine as lithagogue, diuretic, and nephritic. Research on the methanol (MeOH) extract of flowers of H. plicatum DC. subsp. plicatum resulted in the isolation of eight known compounds (1–8). The chemical structures of the compounds were determined as β-sitosterol (1), apigenin (2), nonacosanoic acid (3), astragalin (4), β-sitosterol-3-O-β-D-glucopyranoside (5), helichrysin A (6), helichrysin B (7), and isosalipurposide (8) by spectroscopic and chromatographic/spectrometric methods, including 1D and 2D nuclear magnetic resonance and liquid chromatography-tandem mass spectrometry. Nonacosanoic acid (3) was isolated for the first time from H. plicatum DC. subsp. plicatum. The MeOH extract and isolated compounds were evaluated for their in vitro human carbonic anhydrase I (hCAI) and II (hCAII), acetylcholinesterase (AChE), butyrylcholinesterase (BChE), and α-glycosidase inhibitory activities. The IC50 values of H. plicatum DC. subsp. plicatum MeOH extract for hCAI, hCAII, AChE, BChE, and α-glycosidase were found to be 77.87, 52.90, 115.50, 117.46, and 81.53 mg/mL, respectively. The compounds showed IC50 values of 1.43–4.47, 1.40–4.32, 1.69–2.90, 1.09–3.89, and 1.61–3.80 μM against hCAI, hCAII, AChE, BChE, and α-glycosidase, respectively. In summary, H. plicatum DC. subsp. plicatum secondary metabolites demonstrated strong inhibitory effects especially against hCAI and hCAII, whereas the MeOH extract showed a weak inhibitory effect on all enzymes.

scholarly journals The influence of plant essential oils on in vitro growth of Pectobacterium and Dickeya spp. bacteria

2021 ◽  
Vol 20 (6) ◽  
pp. 19-29
Author(s):  
Małgorzata Schollenberger ◽  
Agnieszka Gadomska-Gajadhur ◽  
Ewa Mirzwa-Mróz ◽  
Damian Kret ◽  
Ewa Skutnik ◽  
...  
Keyword(s):  
Essential Oils ◽  
Soft Rot ◽  
Inhibitory Effect ◽  
Diffusion Method ◽  
Juniperus Virginiana ◽  
Bacterial Strains ◽  
Disk Diffusion Method ◽  
First Report ◽  
Plant Essential Oils

 The activity of essential oils from Eucalyptus globulus, Pinus silvestris, Lavandula angustifolia, Juniperus virginiana, Rosmarinus officinalis and Citrus paradise against the soft-rot pathogens Pectobacterium carotovorum subsp. carotovorum, Pectobacterium atrosepticum, Pectobacterium parmentieri and Dickeya solani was determined in vitro. The antibacterial activity of the essential oils will be evaluated using the disk-diffusion method by Kirby-Bauer [Bauer et al. 1966]. It was found that all the presented essential oils varied in antimicrobial activity against the four bacterial strains. No differences in the influence of streptomycin on inhibition of growth of the four bacterial strains were observed. Among six tested plants, essential oils from P. sylvestris had the strongest inhibitory effect on the growth of soft rot bacteria from Pectobacterium genus. This paper constitute the first report on the activity of the essential oils obtained from J. virginiana against soft rot bacteria. They are also the first report on the activity of the essential oils obtained from E. globulus, P. silvestris, L. angustifolia and C. paradisi against P. atrosepticum, P. parmentieri and D. solani as well as on the activity of the R. officinalis essential oils against P. atrosepticum and P. parmentieri.

scholarly journals Endophytic Fungi from Alstonia boonei De Wild and Greenwayodendron suaveolens (Engl. and Diels) Verdc. subsp. Suaveolens Possess Inhibitory Activity against Pneumonia Causing Bacteria

2021 ◽  
Vol 2021 ◽  
pp. 1-10
Author(s):  
Paola Cynthia Emoh Demeni ◽  
Patrick Hervé Diboue Betote ◽  
Christelle Wayoue Kom ◽  
Eric Ngalani Tchamgoue ◽  
Esther Del Florence Ndedi Moni ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
Secondary Metabolites ◽  
Endophytic Fungi ◽  
Phytochemical Screening ◽  
Bacterial Strains ◽  
Microdilution Method ◽  
Methanolic Extracts ◽  
Bactericidal Effects ◽  
Inhibition Zones

This study evaluated the antibacterial efficacy of methanolic extracts of isolated endophytic fungi from stem barks and leaves of Alstonia boonei De Wild and Greenwayodendron suaveolens (Engl. and Diels) Verdc. subsp. Suaveolens against Klebsiella pneumoniae ATCC 43816, Haemophilus influenzae ATCC 49247, Pseudomonas aeruginosa ATCC 27853, and Escherichia coli ATCC 35218, responsible for causing pneumonia. The endophytic fungi were isolated and characterized in the Potato Dextrose Agar (PDA), Sabouraud Dextrose Agar (SDA), and Czapek Dox Agar (CDA) media. The fungi and their methanolic extracts were tested for in vitro antibacterial potential by antagonistic assay for endophytic fungi against bacterial pathogens and microdilution method. The phytochemical screening of extracts was carried out according to the colorimetric and precipitation methods to reveal the presence of secondary metabolites. The results showed that 24 macroscopically and microscopically distinct endophytic fungi were isolated, identified, and stored. These endophytic fungi possessed antibacterial activity against the selected bacterial strains with inhibition zones ranging from 7.00 to 25.00 mm. The endophytic fungi GS15 and AB24 have presented the inhibitions zones of 20.33 mm and 25.00 mm, respectively, and these were better than the ones obtained for Levofloxacin®. The endophytes with inhibition zones greater than 10 mm were used for extraction of their secondary metabolites. The endophytic fungi extracts showed antibacterial activity with the minimum inhibitory concentrations (MICs) ranging from 6.25 × 10−4 to 2 × 10−2 g/L and the minimum bactericidal concentrations (MBCs) ranging from 2.5 × 10−3 to 2 × 10−2 g/L. The endophytic fungi GS15 extract was the most effective extract; it showed bactericidal effects on the tested bacterial strains. The phytochemical screening of the extracts revealed the presence of secondary metabolites classes, responsible for causing the obtained antibacterial activity. Thus, the endophytic fungi methanolic extracts from A. boonei and G. suaveolens have the potential to inhibit the growth of bacteria responsible for nosocomial pneumonia.

scholarly journals Autoinducer 2 Affects Biofilm Formation by Bacillus cereus

2006 ◽  
Vol 72 (1) ◽  
pp. 937-941 ◽  
Author(s):  
Sandrine Auger ◽  
Evelyne Krin ◽  
Stéphane Aymerich ◽  
Michel Gohar
Keyword(s):  
Bacillus Cereus ◽  
Biofilm Formation ◽  
Inhibitory Effect ◽  
Strain Atcc ◽  
Photorhabdus Luminescens ◽  
Autoinducer 2 ◽  
Exogenous Addition

ABSTRACT Cell-free supernatants from growing Bacillus cereus strain ATCC 10987 induced luminescence in a Photorhabdus luminescens ΔluxS mutant, indicating the production of functional autoinducer 2 (AI-2). The exogenous addition of in vitro synthesized AI-2 had an inhibitory effect on biofilm formation by B. cereus and promoted release of the cells from a preformed biofilm.

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