scholarly journals Effects of Aeration Flow Rate in the Culture Medium on the Growth Performance and Egg Production of Copepod Oithona similis Fed with Fermented Organic Diet

2020 ◽  
Vol 147 ◽  
pp. 01006
Author(s):  
Diana Chilmawati ◽  
Johannes Hutabarat ◽  
Sutrisno Anggoro ◽  
Suminto Suminto
Keyword(s):  
Growth Performance ◽  
Flow Rate ◽  
Egg Production ◽  
Culture Medium ◽  
Culture Media ◽  
Fish Larvae ◽  
Individual Growth ◽  
Total Density ◽  
Oithona Similis ◽  
The Individual

The availability of copepod Oithona similis as live food organism for shrimp and marine fish larvae is strongly influenced by the optimum feed and environment conditions. Optimization of dissolved oxygen (DO) for O. similis can be carried out by providing proper aeration in culture media. Feeding with fermented organic diet besides phytoplankton is expected to support the individual growth, metabolism, and reproduction of O. similis. The aim of this study was to examine the effect of different aeration flow rates on the growth performance and egg production of O. similis and to determine the optimum aeration flow rate. Completely Randomized Design Experiment used in this study with 4 treatments and 4 replications. The treatment was O. similis culture with different aeration flow rate of 0.00; 22.00; 45.67; 66.67 mL.second-1. The results showed that the difference aeration flow rate significantly affected (p <0.05) the growth performance and egg production of O. similis. The aeration flow rate of 45.67 mL.second-1 provided the best growth performance with total density of 81.25 ± 2.99 ind.mL-1; population specific growth rate 0.220 ± 0.002 day-1; and egg production28.40 ± 0.48 eggs.ind-1. Moreover, the optimum aeration flow rate culture medium for O. similis is 45.70 mL.second-1.

scholarly journals Organic Feed Enrichment Effects toward Growth Performance and Egg Production of Oithona similis

Omni-Akuatika ◽  
2020 ◽  
Vol 16 (3) ◽  
pp. 128
Author(s):  
Diana Chilmawati ◽  
Johannes Hutabarat ◽  
Sutrisno Anggoro ◽  
Suminto Suminto
Keyword(s):  
Optimum Condition ◽  
Growth Performance ◽  
Protein Content ◽  
Egg Production ◽  
Culture Media ◽  
Total Density ◽  
Optimum Point ◽  
Oithona Similis ◽  
The Difference ◽  
Feed Protein

The optimum condition of culture media and feed diets which fit the necessities of Oithona similis life (ecophysiological) is very important for it to grow and develop in its maximum. The enrichment of O. similis feed can be done by adding fermented organic feed. Providing organic feed with optimum protein content is necessary to support the growth process and reproduction of O. similis. Osmoregulation process of water organism which is influenced by media salinity is related to bioenergetics process as the effort to use feed energy for the growth. This research aimed to examine the effect of different organic feed protein content and to determine the optimum point which may result in the best growth and egg production of O. similis in the optimum condition of culture media. The study used an experimental method with a completely random design consisting of 4 treatments and 4 repetitions each, i.e. O. similis culture with A. 25%; B. 30%; C. 35%; and D. 40% organic feed protein content. The results showed that the difference of protein content in organic feed affects the growth and production of O. similis eggs. The optimum point of protein content in the fermented organic feed is on protein content B, i.e. 30%, which resulted in the best growth performance (70.44 ± 0.43 ind.mL-1 total density, 0.213 ± 0.001.day-1 population growth rate, and 27.38 ± 0.48 eggs.ind-1 egg production. (ttujuan dan pembahasan harus past tense), Tujuan di abstrak minimal harus menggambarkan atau sama denga tujuan di pendahuluan)Keywords: enrichment, Oithona similis, organic feed, protein, reproduction

scholarly journals Coexistence effect of rotifer, Brachionus rotundiformis and copepod, Oithona similis in culture media on growth performance and eggs production

2019 ◽  
Vol 20 (8) ◽  
Author(s):  
Suminto Suminto ◽  
Diana Chilmawati
Keyword(s):  
Growth Rate ◽  
Population Growth ◽  
Growth Performance ◽  
Mass Culture ◽  
Egg Production ◽  
Population Growth Rate ◽  
Culture Media ◽  
Brachionus Rotundiformis ◽  
Oithona Similis ◽  
Final Density

Abstract. Suminto, Chilmawati D. 2019. Coexistence effect of rotifer, Brachionus rotundiformis and copepod, Oithona similis in culture media on growth performance and eggs production. Biodiversitas 20: 2396-2402. The effect of Brachionus rotundiformis, and Oithonasimilis coexistence in culture media on the growth performance and egg production were examined and it aims to know the best coexistence percentage which provides the highest growth performance and egg production of O. similis and B. rotundiformis. Experiment method was carried out in this research by using a completely randomized design (CRD) with 5 treatments and 4 replications. The treatments were percentage through mix culture ratio of O.similis. and B. rotundiformis whereas those treatments were with ratio of 100% and 0% (A); 90% and 10% (B); 80% and 20% (C); 70% and 30% (D); and 60% and 40% (E), respectively. The results showed that, B. rotundiformis had a significant effect (p <0.05) on final density, population growth rate and eggs production of Oithona sp.; coexistence of 60% Oithona sp. and 40% B. rotundiformis provides the best final density for Oithona sp. (27.40±0.34 ind./ml) and B. rotundiformis (30.23±0.21 ind./ml); the highest population growth rate of Oithona sp. (0.29±0.02 ind./day) and B. rotundiformis (0.36±0.03 ind./day); and the highest egg production of Oithona sp. (18.83±0.76 eggs/ind) and B. rotundiformis (3.30±0.24 eggs/ind.). However, the mix culture of O.similis. and B. Rotundiformis may be applied in co-existent semi-mass or mass culture.

Morphogenesis of protoplasts of Entomophthora egressa in simplified culture media

1977 ◽  
Vol 55 (24) ◽  
pp. 3046-3053 ◽  
Author(s):  
Gary B. Dunphy ◽  
Richard A. Nolan
Keyword(s):  
Culture Medium ◽  
Culture Media ◽  
Growth Cycle ◽  
Protoplast Regeneration ◽  
Liquid Media ◽  
Insect Tissue Culture ◽  
Hyphal Bodies ◽  
The Media ◽  
The Individual ◽  
Mycelial Development

Protoplasts of Entomophthora egressa MacLeod and Tyrrell were grown in Grace's medium and in two simplified liquid media. These latter media contained reduced numbers of amino acids as compared with Grace's insect tissue culture medium. The pattern of protoplast regeneration was the same in all three media used, but additional stages in the morphogenetic sequence werefound. A spherical mesoprotoplast and an elliptical mesoprotoplast stage with actively moving filopodia were detected early in the growth cycle. The clumping of rod-shaped and spherical hyphal bodies was observed. Mycelial development was very pronounced by 89 h of incubation. The osmolarity and pH of the media were unchanged before this time. The individual hyphae were predominantly initiated by spherical hyphal bodies.All stages of regeneration produced spindle-shaped protoplasts when added to fresh media. The inoculation of spent media with spindle-shaped protoplasts favoured an accelerated morpho-genesis of the cells through the presently reported stages.

Pertumbuhan Dan Perkembangan Anggrek Dendrobium anosmum Pada Media Kultur In Vitro Dengan Beberapa Konsentrasi Air Kelapa

Agrologia ◽  
2018 ◽  
Vol 1 (1) ◽  
Author(s):  
S. Tuhuteru ◽  
Meity L Hehanussa ◽  
Simon H.T Raharjo
Keyword(s):  
Culture Medium ◽  
Culture Media ◽  
Water Concentration ◽  
Medium Composition ◽  
Coconut Water ◽  
Orchid Species ◽  
Randomized Design ◽  
Wet Weight ◽  
Completely Randomized Design

Dendrobium anosmum is one of natural orchids in Indonesia. Optimization of medium composition for orchid propagation through in vitro culture is necessary to enhance propagule multiplication capabilities and quality. This study was aimed to study the influence of concentration of coconut water in culture medium on in vitro growth and development of D. anosmum orchid species and to determine the optimal coconut water concentration in culture media.  The experiment were arranged in a Completely Randomized Design with four treatments and eight replications. The treatments consisted of the addition of coconut water with concentrations: 0 ml•l -1 (control), 50 ml•l-1, 100 ml•l-1 and 150 ml•l-1. The results showed that addition of coconut water in culture medium gave different effect on shoot growth and multiplication of D. anosmum orchids.  Coconut water concentration of 100 ml•l-1 was the best concentration for growth and multiplication of D. anosmum orchids, based on both shoots and roots growth, plantlet height and wet weight.

scholarly journals EFFECT OF TUBE PITCH ON HEAR TRANSFER IN SPRINKLED TUBE BUNDLE

2015 ◽  
Vol 55 (5) ◽  
pp. 329 ◽  
Author(s):  
Petr Kracík ◽  
Jiří Pospíšil
Keyword(s):  
Flow Rate ◽  
Tube Bundle ◽  
Thin Liquid Film ◽  
Thermal Conditions ◽  
Tube Length ◽  
Falling Film ◽  
Constant Flow Rate ◽  
Physical Conditions ◽  
The Individual ◽  
Tube Pitch

Water flowing on a sprinkled tube bundle forms three basic modes: the Droplet mode (the liquid drips from one tube to another), the Jet mode (with an increasing flow rate, the droplets merge into a column) and the Membrane (Sheet) mode (with a further increase in the flow rate of the falling film liquid, the columns merge and create sheets between the tubes. With a sufficient flow rate, the sheets merge at this stage, and the tube bundle is completely covered by a thin liquid film). There are several factors influencing both the individual modes and the heat transfer. Beside the above-mentioned falling film liquid flow rate, these are for instance the tube diameters, the tube pitches in the tube bundle, or the physical conditions of the falling film liquid. This paper presents a summary of data measured at atmospheric pressure, with a tube bundle consisting of copper tubes of 12 millimetres in diameter, and with a studied tube length of one meter. The tubes are situated horizontally one above another at a pitch of 15 to 30 mm, and there is a distribution tube placed above them with water flowing through apertures of 1.0mm in diameter at a 9.2mm span. Two thermal conditions have been tested with all pitches: 15 °C to 40 °C and 15 °C to 45 °C. The temperature of the falling film liquid, which was heated during the flow through the exchanger, was 15 °C at the distribution tube input. The temperature of the heating liquid at the exchanger input, which had a constant flow rate of approx. 7.2. litres per minute, was 40 °C, or alternatively 45 °C.

scholarly journals Untargeted Metabolomics Approach for the Discovery of Environment-Related Pyran-2-ones Chemodiversity in a Marine-Sourced Penicillium restrictum

Marine Drugs ◽  
2021 ◽  
Vol 19 (7) ◽  
pp. 378
Author(s):  
Van-Tuyen Le ◽  
Samuel Bertrand ◽  
Thibaut Robiou du Pont ◽  
Fabrice Fleury ◽  
Nathalie Caroff ◽  
...  
Keyword(s):  
Culture Medium ◽  
Blue Mussel ◽  
Culture Media ◽  
Principal Component ◽  
Chemical Diversity ◽  
Untargeted Metabolomics ◽  
High Chemical ◽  
Medium Mass ◽  
Metabolomics Study ◽  
Penicillium Restrictum

Very little is known about chemical interactions between fungi and their mollusc host within marine environments. Here, we investigated the metabolome of a Penicillium restrictum MMS417 strain isolated from the blue mussel Mytilus edulis collected on the Loire estuary, France. Following the OSMAC approach with the use of 14 culture media, the effect of salinity and of a mussel-derived medium on the metabolic expression were analysed using HPLC-UV/DAD-HRMS/MS. An untargeted metabolomics study was performed using principal component analysis (PCA), orthogonal projection to latent structure discriminant analysis (O-PLSDA) and molecular networking (MN). It highlighted some compounds belonging to sterols, macrolides and pyran-2-ones, which were specifically induced in marine conditions. In particular, a high chemical diversity of pyran-2-ones was found to be related to the presence of mussel extract in the culture medium. Mass spectrometry (MS)- and UV-guided purification resulted in the isolation of five new natural fungal pyran-2-one derivatives—5,6-dihydro-6S-hydroxymethyl-4-methoxy-2H-pyran-2-one (1), (6S, 1’R, 2’S)-LL-P880β (3), 5,6-dihydro-4-methoxy-6S-(1’S, 2’S-dihydroxy pent-3’(E)-enyl)-2H-pyran-2-one (4), 4-methoxy-6-(1’R, 2’S-dihydroxy pent-3’(E)-enyl)-2H-pyran-2-one (6) and 4-methoxy-2H-pyran-2-one (7)—together with the known (6S, 1’S, 2’S)-LL-P880β (2), (1’R, 2’S)-LL-P880γ (5), 5,6-dihydro-4-methoxy-2H-pyran-2-one (8), (6S, 1’S, 2’R)-LL-P880β (9), (6S, 1’S)-pestalotin (10), 1’R-dehydropestalotin (11) and 6-pentyl-4-methoxy-2H-pyran-2-one (12) from the mussel-derived culture medium extract. The structures of 1-12 were determined by 1D- and 2D-MMR experiments as well as high-resolution tandem MS, ECD and DP4 calculations. Some of these compounds were evaluated for their cytotoxic, antibacterial, antileishmanial and in-silico PTP1B inhibitory activities. These results illustrate the utility in using host-derived media for the discovery of new natural products.

scholarly journals Rheological and Microstructural Features of Plant Culture Media Doped with Biopolymers: Influence on the Growth and Physiological Responses of In Vitro-Grown Shoots of Thymus lotocephalus

2021 ◽  
Vol 2 (2) ◽  
pp. 538-553
Author(s):  
Natacha Coelho ◽  
Alexandra Filipe ◽  
Bruno Medronho ◽  
Solange Magalhães ◽  
Carla Vitorino ◽  
...  
Keyword(s):  
In Vitro Culture ◽  
Culture Medium ◽  
Culture Media ◽  
Average Pore Size ◽  
Physiological Responses ◽  
Gum Arabic ◽  
Shoot Elongation ◽  
Hydroxyethyl Cellulose ◽  
Plant Culture

In vitro culture is an important biotechnological tool in plant research and an appropriate culture media is a key for a successful plant development under in vitro conditions. The use of natural compounds to improve culture media has been growing and biopolymers are interesting alternatives to synthetic compounds due to their low toxicity, biodegradability, renewability, and availability. In the present study, different culture media containing one biopolymer (chitosan, gum arabic) or a biopolymer derivative [hydroxyethyl cellulose (HEC), carboxymethyl cellulose (CMC)], at 100 or 1000 mg L−1, were tested regarding their influence on the growth and physiological responses of Thymus lotocephalus in vitro culture. Cellulose-based biopolymers (HEC and CMC) and gum arabic were used for the first time in plant culture media. The results showed that CMC at 100 mg L−1 significantly improved shoot elongation while chitosan, at the highest concentration, was detrimental to T. lotocephalus. Concerning only the evaluated physiological parameters, all tested biopolymers and biopolymer derivatives are safe to plants as there was no evidence of stress-induced changes on T. lotocephalus. The rheological and microstructural features of the culture media were assessed to understand how the biopolymers and biopolymer derivatives added to the culture medium could influence shoot growth. As expected, all media presented a gel-like behaviour with minor differences in the complex viscosity at the beginning of the culture period. Most media showed increased viscosity overtime. The surface area increased with the addition of biopolymers and biopolymer derivatives to the culture media and the average pore size was considerably lower for CMC at 100 mg L−1. The smaller pores of this medium might be related to a more efficient nutrients and water uptake by T. lotocephalus shoots, leading to a significant improvement in shoot elongation. In short, this study demonstrated that the different types of biopolymers and biopolymer derivatives added to culture medium can modify their microstructure and at the right concentrations, are harmless to T. lotocephalus shoots growing in vitro, and that CMC improves shoot length.

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