scholarly journals Medium optimization for exopolysaccharides production by Bacillus Zhangzhouensis BZ 16 strain isolated from Khnifiss Lagoon

2021 ◽  
Vol 234 ◽  
pp. 00099
Author(s):  
Mouna Miri ◽  
Hafida Bergayou ◽  
Ahmed Belmouden ◽  
Abdellatif Moukrim ◽  
Hakim Baazizi ◽  
...  
Keyword(s):  
Yeast Extract ◽  
Medium Optimization ◽  
Industrial Applications ◽  
Medium Composition ◽  
Natural Polymers ◽  
Extreme Habitats ◽  
Phosphate Source ◽  
Industrial Level ◽  
Optimal Medium ◽  
Exopolysaccharides Production

The increased demand for natural polymers for diverse industrial applications in last years has led to a renovated interest in exopolysaccharides (EPS) production by microorganisms. Ecological roles of EPS especially for those isolated from extreme habitats such as hypersaline ponds have been reported in several studies. A moderate halophilic strain producing exopolysaccharide (EPS) has been isolated earlier. However, the EPS production of the strain (2 g/L) was relatively low to exploit it at the industrial level. In the present work, the strain was identified as Bacillus zhangzhouensis and named as BZ 16 and the optimal medium was studied to boost the EPS production. Maximum EPS production was obtained in medium with 125 g/L sucrose, 30 g/L yeast extract. Phosphate source inhibited EPS production even if it increased the growth. Under optimal medium composition, EPS was produced at 12.37 g/L, which was 6 times greater than the production yield achievable without optimizing conditions.

scholarly journals Isolation, Identification and Characterization of Bioflocculant-Producing Bacteria from Activated Sludge of Vulindlela Wastewater Treatment Plant

2021 ◽  
Vol 1 (3) ◽  
pp. 586-606
Author(s):  
Nkanyiso Celukuthula Nkosi ◽  
Albertus K. Basson ◽  
Zuzingcebo G. Ntombela ◽  
Tsolanku S. Maliehe ◽  
Rajasekhar V. S. R. Pullabhotla
Keyword(s):  
Activated Sludge ◽  
Treatment Plant ◽  
16S Rrna Gene Sequencing ◽  
Industrial Applications ◽  
Culture Conditions ◽  
Inoculum Size ◽  
Medium Composition ◽  
Rrna Gene ◽  
Sequencing Analysis ◽  
Optimal Medium

The low microbial flocculant yields and efficiencies limit their industrial applications. There is a need to identify bacteria with high bioflocculant production. The aim of this study was to isolate and identify a bioflocculant-producing bacterium from activated sludge wastewater and characterise its bioflocculant activity. The identification of the isolated bacterium was performed by 16S rRNA gene sequencing analysis. The optimal medium composition (carbon and nitrogen sources, cations and inoculum size) and culture conditions (temperature, pH, shaking speed and time) were evaluated by the one-factor-at-a-time method. The morphology, functional groups, crystallinity and pyrolysis profile of the bioflocculant were analysed using scanning electron microscope (SEM), Fourier transform infrared (FTIR) and thermogravimetric (TGA) analysis. The bacterium was identified as Proteus mirabilis AB 932526.1. Its optimal medium and culture conditions were: sucrose (20 g/L), yeast extract (1.2 g/L), MnCl2 (1 g/L), pH 6, 30 °C, inoculation volume (3%), shaking speed (120 rpm) for 72 h of cultivation. SEM micrograph revealed the bioflocculant to be amorphous. FTIR analysis indicated the presence of hydroxyl, carboxyl and amino groups. The bioflocculant was completely pyrolyzed at temperatures above 800 °C. The bacterium has potential to produce bioflocculant of industrial importance.

scholarly journals Statistical Optimization of the Medium Composition by Response Surface Methodology to Enhance Schizophyllan Production by Schizophyllum commune

2011 ◽  
Vol 66 (3-4) ◽  
pp. 173-181 ◽  
Author(s):  
Wenbing Li ◽  
Pengpeng Zhou ◽  
Longjiang Yu
Keyword(s):  
Response Surface Methodology ◽  
Response Surface ◽  
Yeast Extract ◽  
Schizophyllum Commune ◽  
Fermentation Medium ◽  
Medium Composition ◽  
Excellent Correlation ◽  
Predicted Values ◽  
Optimal Medium ◽  
Central Composite

The response surface methodology (RSM) involving central composite design (CCD) was employed to optimize the fermentation medium for the cell growth and schizophllan production by Schizophyllum commune CGMCC 5.113 in submerged culture at pH 6.5 and 26°C. The four variables involved in this study were glucose, yeast extract, ammonium nitrate, and magnesium sulfate. The statistical analysis of the results showed that, in the range studied, glucose and yeast extract had a highly significant effect on schizophyllan production. The optimal medium for schizophyllan production calculated from the regression model of RSM was as follows: glucose, 18 g/l; yeast extract, 0.5 g/l; NH4NO3, 0.48 g/l; and MgSO4, 0.05 g/l, with a predicted maximum schizophyllan production of 11.74 g/l. These predicted values were experimentally validated. The excellent correlation between predicted and measured values justifies the validity of the response model. The results of bioreactor fermentation also show that the optimized medium enhanced schizophyllan production (12.80 g/l) by S. commune in a 5-l fermenter

Effect of Different Nutrient Components on Polysaccharide and Biomass Production from Fomitopsis pinicola Karst

2012 ◽  
Vol 503-504 ◽  
pp. 174-177
Author(s):  
Li Min Hao ◽  
Zheng Li ◽  
Ai Li Zhao ◽  
Wei Long Chen ◽  
Zi Tao Wang
Keyword(s):  
Biomass Production ◽  
Yeast Extract ◽  
Biomass Growth ◽  
Fomitopsis Pinicola ◽  
Potential Applications ◽  
Optimal Medium

The Fomitopsis pinicola Karst is a novel mushroom. Its exo-polysaccharide and biomass of F.pinicola Karst have widely potential applications. In this paper, effect of different nutrient components on exo-polysaccharide and biomass production was reported. The results revealed that the optimal medium for producing CEPS was (g/L): glucose 150, yeast extract 5, MgSO4•7H2O 0.8, KH2PO4 1.2. The optimal medium for biomass growth was (g/L): glucose 150, yeast extract 15, MgSO4•7H2O 0.6, KH2PO4 1.4.

scholarly journals Jacob’s ladder Polemonium caeruleum L. and black salsify Sсorzonera hispanica L. in vitro culture

2018 ◽  
Vol 22 ◽  
pp. 216-221
Author(s):  
O. V. Bulko ◽  
L. G. Lioshina
Keyword(s):  
In Vitro Culture ◽  
Root Culture ◽  
Medium Composition ◽  
Cultivation Medium ◽  
Plant Cultivation ◽  
Regenerated Plants ◽  
Composition Modification ◽  
Jacob's Ladder ◽  
Optimal Medium

Aim. Micropropagation of Jacob’s ladder Polemonium caeruleum L. and black salsify Scorzonera hispanica L., obtaining root culture and regenerated plants. Methods. In vitro plant cultivation, medium composition modification for micropropagation, inoculation of explants with agrobacterial strains. Results. In vitro cultures of Jacob’s ladder and black salsify have been obtained, the optimal medium composition has been determined for the effective plants multiplication, rooting and growth, root cultures and regenerated plants of studied species have been obtained. Conclusions. Obtained technology of in vitro culture establishment of P. caeruleum and S. hispanica can be used for plants microclonal propagation so as root culture and regenerated plants acquiring due to the agrobacterial transformation – for further studies of secondary metabolism of these plants. Keywords: P. caeruleum L., S. hispanica L., micropropagation, phytohormones, root culture.

scholarly journals Optimization of an Economical Medium Composition For The Coculture of Clostridium Butyricum And Bacillus Coagulans

2021 ◽  
Author(s):  
Yonghong Li ◽  
Yun Wang ◽  
Yingying Liu ◽  
Xuan Li ◽  
Keke Li ◽  
...  
Keyword(s):  
Industrial Production ◽  
Fermentation Process ◽  
Bacillus Coagulans ◽  
Medium Composition ◽  
Clostridium Butyricum ◽  
Production Level ◽  
Beneficial Bacteria ◽  
Spore Yield ◽  
Viable Bacteria ◽  
Optimal Medium

Abstract Clostridium butyricum is a spore-forming probiotic existing in the intestines of humans and animals which can promote the enhancement of beneficial bacteria and maintain intestinal microecological balance. However, it is difficult to improve the production level of C. butyricum by conventional fermentation process. In this study, a co-fermentation process of C. butyricum DL-1 and Bacillus coagulans ZC2-1 was established to improve the viable counts and spore yield of C. butyricum, and the formula of coculture medium was optimized by flask fermentation. The results showed that the optimum medium composition is bran 10 g/L, corn steep powder 15 g/L, peptone 15 g/L, K2HPO4 1 g/L and MnSO4 0.5 g/L. Cultured stationarily in the optimal medium for 36 h, the number of viable bacteria of C. butyricum DL-1 reached 1.5×108 CFU/mL and the spore forming rate was 92.6%. The results revealed an economical and effective medium composition for the coculture of C. butyricum and B. coagulans. The co-fermentation process established in this study provides a new fermentation mode for the industrial production of other absolute anerobic bacteria.

Plackett-Burman Design for Media Nutrients of Biocontrol Lysinibacillus xylanilyticus BPM1 against Aflatoxin

2014 ◽  
Vol 522-524 ◽  
pp. 295-298
Author(s):  
Kai Wang ◽  
Pei Sheng Yan ◽  
Li Xin Cao
Keyword(s):  
Biological Control ◽  
Yeast Extract ◽  
Antagonistic Activity ◽  
Medium Composition ◽  
Peanut Hulls ◽  
Two Factors ◽  
Burman Design ◽  
Soya Peptone ◽  
Plackett Burman Design ◽  
Biocontrol Bacterium

Aflatoxins (AFs) are a series of highly toxic and carcinogenic secondary metabolites. In order to eliminate AFs contamination, biological control is one of the more promising techniques. In this study, we describe the optimization of media nutrients for the selected biocontrol bacterium, Lysinibacillus xylanilyticus strain BPM1. The strain was isolated from the peanut hulls in Shandong Province, China and exhibited antagonistic activity against aflatoxins. Maltose and sucrose were identified as best carbon source, while soya peptone and yeast extract as nitrogen source led to the highest OD600 observations. Medium composition was optimized using Plackett-Burman design, which was applied to find the key ingredients. The results revealed that the most significant two factors which were more effective in the fermentation of L. xylanilyticus BPM1 were soya peptone and yeast extract.

Fungal Exopolysaccharides: Production and Biotechnological Industrial Applications in Food and Allied Sectors

2021 ◽  
pp. 311-357
Author(s):  
Pavidharshini Selvasekaran ◽  
Mahalakshmi ◽  
Felicia Roshini ◽  
Lavanya Agnes Angalene ◽  
Chandini ◽  
...  
Keyword(s):  
Industrial Applications ◽  
Exopolysaccharides Production

scholarly journals Experimental Design to Improve Cell Growth and Ethanol Production in Syngas Fermentation by Clostridium carboxidivorans

Catalysts ◽  
2020 ◽  
Vol 10 (1) ◽  
pp. 59 ◽  
Author(s):  
Carolina Benevenuti ◽  
Alanna Botelho ◽  
Roberta Ribeiro ◽  
Marcelle Branco ◽  
Adejanildo Pereira ◽  
...  
Keyword(s):  
Cell Growth ◽  
Ethanol Production ◽  
Yeast Extract ◽  
Biomass Gasification ◽  
Culture Collection ◽  
Medium Composition ◽  
Syngas Fermentation ◽  
The Cost ◽  
Clostridium Carboxidivorans ◽  
Peptone Yeast Extract Glucose

Fermentation of gases from biomass gasification, named syngas, is an important alternative process to obtain biofuels. Sequential experimental designs were used to increase cell growth and ethanol production during syngas fermentation by Clostridium carboxidivorans. Based on ATCC (American Type Culture Collection) 2713 medium composition, it was possible to propose a best medium composition for cell growth, herein called TYA (Tryptone-Yeast extract-Arginine) medium and another one for ethanol production herein called TPYGarg (Tryptone-Peptone-Yeast extract-Glucose-Arginine) medium. In comparison to ATCC® 2713 medium, TYA increased cell growth by 77%, reducing 47% in cost and TPYGarg increased ethanol production more than four-times, and the cost was reduced by 31%. In 72 h of syngas fermentation in TPYGarg medium, 1.75-g/L of cells, 2.28 g/L of ethanol, and 0.74 g/L of butanol were achieved, increasing productivity for syngas fermentation.

scholarly journals Enhancement of Versatile Extracellular Cellulolytic and Hemicellulolytic Enzyme Productions by Lactobacillus plantarum RI 11 Isolated from Malaysian Food Using Renewable Natural Polymers

Molecules ◽  
2020 ◽  
Vol 25 (11) ◽  
pp. 2607 ◽  
Author(s):  
Nursyafiqah A. Mohamad Zabidi ◽  
Hooi Ling Foo ◽  
Teck Chwen Loh ◽  
Rosfarizan Mohamad ◽  
Raha Abdul Rahim
Keyword(s):  
Lactobacillus Plantarum ◽  
Yeast Extract ◽  
Palm Kernel ◽  
Basal Medium ◽  
Optimization Approach ◽  
Palm Kernel Cake ◽  
Natural Polymers ◽  
Cell Biomass ◽  
Specific Activities ◽  
Hemicellulolytic Enzyme

Lactobacillus plantarum RI 11 was reported recently to be a potential lignocellulosic biomass degrader since it has the capability of producing versatile extracellular cellulolytic and hemicellulolytic enzymes. Thus, this study was conducted to evaluate further the effects of various renewable natural polymers on the growth and production of extracellular cellulolytic and hemicellulolytic enzymes by this novel isolate. Basal medium supplemented with molasses and yeast extract produced the highest cell biomass (log 10.51 CFU/mL) and extracellular endoglucanase (11.70 µg/min/mg), exoglucanase (9.99 µg/min/mg), β-glucosidase (10.43 nmol/min/mg), and mannanase (8.03 µg/min/mg), respectively. Subsequently, a statistical optimization approach was employed for the enhancement of cell biomass, and cellulolytic and hemicellulolytic enzyme productions. Basal medium that supplemented with glucose, molasses and soybean pulp (F5 medium) or with rice straw, yeast extract and soybean pulp (F6 medium) produced the highest cell population of log 11.76 CFU/mL, respectively. However, formulated F12 medium supplemented with glucose, molasses and palm kernel cake enhanced extracellular endoglucanase (4 folds), exoglucanase (2.6 folds) and mannanase (2.6 folds) specific activities significantly, indicating that the F12 medium could induce the highest production of extracellular cellulolytic and hemicellulolytic enzymes concomitantly. In conclusion, L. plantarum RI 11 is a promising and versatile bio-transformation agent for lignocellulolytic biomass.

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