scholarly journals Optimization of an Economical Medium Composition For The Coculture of Clostridium Butyricum And Bacillus Coagulans

2021 ◽  
Author(s):  
Yonghong Li ◽  
Yun Wang ◽  
Yingying Liu ◽  
Xuan Li ◽  
Keke Li ◽  
...  
Keyword(s):  
Industrial Production ◽  
Fermentation Process ◽  
Bacillus Coagulans ◽  
Medium Composition ◽  
Clostridium Butyricum ◽  
Production Level ◽  
Beneficial Bacteria ◽  
Spore Yield ◽  
Viable Bacteria ◽  
Optimal Medium

Abstract Clostridium butyricum is a spore-forming probiotic existing in the intestines of humans and animals which can promote the enhancement of beneficial bacteria and maintain intestinal microecological balance. However, it is difficult to improve the production level of C. butyricum by conventional fermentation process. In this study, a co-fermentation process of C. butyricum DL-1 and Bacillus coagulans ZC2-1 was established to improve the viable counts and spore yield of C. butyricum, and the formula of coculture medium was optimized by flask fermentation. The results showed that the optimum medium composition is bran 10 g/L, corn steep powder 15 g/L, peptone 15 g/L, K2HPO4 1 g/L and MnSO4 0.5 g/L. Cultured stationarily in the optimal medium for 36 h, the number of viable bacteria of C. butyricum DL-1 reached 1.5×108 CFU/mL and the spore forming rate was 92.6%. The results revealed an economical and effective medium composition for the coculture of C. butyricum and B. coagulans. The co-fermentation process established in this study provides a new fermentation mode for the industrial production of other absolute anerobic bacteria.

scholarly journals Impact of media components from different suppliers on enterokinase productivity in Pichia pastoris

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Ján Krahulec ◽  
Martin Šafránek
Keyword(s):  
Recombinant Proteins ◽  
Fermentation Process ◽  
High Impact ◽  
Production Level ◽  
Media Component ◽  
Component Supplier ◽  
The Media ◽  
The Difference ◽  
Optimisation Procedure ◽  
The Ideal

Abstract Background The aim of this study was to provide an information about the homogeneity on the level of enterokinase productivity in P. pastoris depending on different suppliers of the media components. Results In previous studies, we performed the optimisation process for the production of enterokinase by improving the fermentation process. Enterokinase is the ideal enzyme for removing fusion partners from target recombinant proteins. In this study, we focused our optimization efforts on the sources of cultivation media components. YPD media components were chosen as variables for these experiments. Several suppliers for particular components were combined and the optimisation procedure was performed in 24-well plates. Peptone had the highest impact on enterokinase production, where the difference between the best and worst results was threefold. The least effect on the production level was recorded for yeast extract with a 1.5 fold difference. The worst combination of media components had a activity of only 0.15 U/ml and the best combination had the activity of 0.88 U/ml, i.e., a 5.87 fold difference. A substantially higher impact on the production level of enterokinase was observed during fermentation in two selected media combinations, where the difference was almost 21-fold. Conclusions Results demonstrated in the present study show that the media components from different suppliers have high impact on enterokinase productivity and also provide the hypothesis that the optimization process should be multidimensional and for achieving best results it is important to perform massive process also in terms of the particular media component supplier .

scholarly journals Jacob’s ladder Polemonium caeruleum L. and black salsify Sсorzonera hispanica L. in vitro culture

2018 ◽  
Vol 22 ◽  
pp. 216-221
Author(s):  
O. V. Bulko ◽  
L. G. Lioshina
Keyword(s):  
In Vitro Culture ◽  
Root Culture ◽  
Medium Composition ◽  
Cultivation Medium ◽  
Plant Cultivation ◽  
Regenerated Plants ◽  
Composition Modification ◽  
Jacob's Ladder ◽  
Optimal Medium

Aim. Micropropagation of Jacob’s ladder Polemonium caeruleum L. and black salsify Scorzonera hispanica L., obtaining root culture and regenerated plants. Methods. In vitro plant cultivation, medium composition modification for micropropagation, inoculation of explants with agrobacterial strains. Results. In vitro cultures of Jacob’s ladder and black salsify have been obtained, the optimal medium composition has been determined for the effective plants multiplication, rooting and growth, root cultures and regenerated plants of studied species have been obtained. Conclusions. Obtained technology of in vitro culture establishment of P. caeruleum and S. hispanica can be used for plants microclonal propagation so as root culture and regenerated plants acquiring due to the agrobacterial transformation – for further studies of secondary metabolism of these plants. Keywords: P. caeruleum L., S. hispanica L., micropropagation, phytohormones, root culture.

scholarly journals Clostridium butyricum Protects IPEC-J2 Cells from ETEC K88-Induced Oxidative Damage by Activating the Nrf2/ARE Signaling Pathway

2021 ◽  
Vol 2021 ◽  
pp. 1-13
Author(s):  
Caixia Dou ◽  
Zhiyuan Shang ◽  
Jiayun Qiao ◽  
Yimeng Wang ◽  
Haihua Li
Keyword(s):  
Antioxidant Enzymes ◽  
Oxidative Damage ◽  
Signaling Pathway ◽  
Antioxidant Response ◽  
Clostridium Butyricum ◽  
Enterotoxigenic Escherichia Coli ◽  
Multiple Infection ◽  
Control Group ◽  
Related Factor ◽  
Viable Bacteria

Clostridium butyricum (CB) is a naturally occurring probiotic compound that can alleviate the oxidative damage induced by enterotoxigenic Escherichia coli K88 (ETEC K88) in porcine intestinal epithelial (IPEC-J2) cells. In this study, we investigate the molecular mechanism underlying this effect. Based on cell viability, malondialdehyde (MDA), superoxide dismutase (SOD), glutathione peroxidase (GPX) assessments, the optimal concentration of ETEC K88 was determined to be 1 × 10 3  cfu/mL. Viable bacteria counts in cells pretreated with CB and then infected with ETEC K88 show that CB can adhere to IPEC-J2 cells and that optimal adhesion is achieved at the multiple infection index (MOI) of 50 at 3 h of pretreatment. The results of qPCR indicate that although ETEC significantly decreases the expression levels of antioxidant enzymes regulated by NF-E2-related factor 2 (Nrf2) compared to the control group, CB reverses this effect. To confirm that Nrf2 is directly involved in the mechanism by which CB alleviates oxidative stress, siRNA was used to silence the expression of Nrf2 gene in IPEC-J2 cells. Compared to the NC+ETEC and siRNA+ETEC groups, the expressions of SOD1, SOD2, GPX1, and GPX2 in the NC+CB+ETEC and siRNA+CB+ETEC groups are significantly increased at 12 h and 24 h. This shows that CB can reduce ETEC K88-induced oxidative damage in IPEC-J2 cells by activating the expression of antioxidant enzymes implicated in the Kelch-like ECH-associated protein-1- (Keap1-) Nrf2/antioxidant response element (ARE) signaling pathway.

Optimization of pH conditions to improve the spore production of Clostridium butyricum NN-2 during fermentation process

2020 ◽  
Vol 202 (5) ◽  
pp. 1251-1256
Author(s):  
Suhong Li ◽  
Xiushan Zhang ◽  
Yan Li ◽  
Lu Tao ◽  
Tuoping Li
Keyword(s):  
Fermentation Process ◽  
Spore Production ◽  
Clostridium Butyricum ◽  
Ph Conditions

scholarly journals Enhanced sophorolipid production and effective conversion of waste frying oil using dual lipophilic substrates

2021 ◽  
Author(s):  
yoshihiko Hirata ◽  
Keisuke Igarashi ◽  
Akiko Ueda ◽  
Glen Lelyn Quan
Keyword(s):  
Oleic Acid ◽  
Palm Oil ◽  
Industrial Production ◽  
Fermentation Process ◽  
Palm Olein ◽  
Culture Medium ◽  
Frying Oil ◽  
Waste Frying Oil ◽  
Frying Oils ◽  
Biological Sources

Abstract A highly efficient sophorolipid (SL) fermentation process using dual lipophilic substrates (DLS) was developed. Using DLS consisting of palm oil and oleic acid in the flask fermentation with SL-producing yeast Starmerella bombicola, the productivity and yield of SL improved 2.4- and 1.7-times, in comparison to when using palm olein alone. In the jar fermentation process with DLS, the SL productivity and yield of 127 g/L and 62.4% was achieved in 7 days, with the complete consumption of residual lipophilic substrates in the culture medium. This process was successfully applied to the effective conversion of waste frying oil to SL, maintaining a high SL productivity and yield of 142.8 g/L and 72% in 9 days. It is concluded that the newly developed fermentation process with DLS can be useful for industrial production of SL from renewable biological sources, especially waste frying oils.

Development of a Shelf-Stable Banana Purée by Combined Factors: Microbial Stability

1994 ◽  
Vol 57 (10) ◽  
pp. 902-907 ◽  
Author(s):  
S. GUERRERO ◽  
S. M. ALZAMORA ◽  
L. N. GERSCHENSON
Keyword(s):  
Heat Treatment ◽  
Ascorbic Acid ◽  
Water Activity ◽  
Bacillus Coagulans ◽  
Clostridium Butyricum ◽  
Potassium Sorbate ◽  
Clostridium Pasteurianum ◽  
Sodium Bisulphite ◽  
Microbial Stability ◽  
Shelf Stable

A preservation process based on the “hurdle” effect for obtaining a shelf-stable banana purée was developed. The microbial stability of the purée was challenged with inoculation of osmophilic and non-osmophilic yeasts, various molds, Bacillus coagulans, Clostridium pasteurianum and Clostridium butyricum. It was shown that growth of both native and inoculated flora may be prevented for at least 120 days storage in banana purée by adjusting water activity (aw) to 0.97, pH to 3.4, adding 250 ppm of ascorbic acid (AA), 100 ppm of potassium sorbate (KS) and 400 ppm of sodium bisulphite (NaHSO3), and applying a mild heat treatment.

scholarly journals Modulation of the Intestinal Microbiota by the Early Intervention with Clostridium Butyricum in Muscovy Ducks

Antibiotics ◽  
2021 ◽  
Vol 10 (7) ◽  
pp. 826
Author(s):  
Xingning Xiao ◽  
Zixian Fu ◽  
Na Li ◽  
Hua Yang ◽  
Wen Wang ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Early Intervention ◽  
Growth Performance ◽  
Intestinal Microbiota ◽  
Short Chain Fatty Acids ◽  
Clostridium Butyricum ◽  
Beneficial Bacteria ◽  
Microbial Composition ◽  
Positive Effects ◽  
Muscovy Ducks

This study evaluated the effects of early intervention with Clostridium butyricum (C. butyricum) on shaping the intestinal microbiota of Muscovy ducklings. A total of 160 1-day-old male ducks were randomly divided into two groups: the CB group was administered with 1 mL of C. butyricum (2 × 109 CFU/mL), while the C group was given 1 mL of saline. The administration lasted for 3 days. We found that C. butyricum had no significant effect on growth performance. The results indicated that inoculation with C. butyricum could significantly increase the abundance of genera Bacteroides, Lachnospiraceae_uncultured, and Ruminococcaceae on Day 14 and reduce the abundance of Escherichia–Shigella and Klebsiella on Days 1 and 3. Moreover, the CB group ducks had higher concentrations of acetic, propionic, and butyrate in the cecum than the C group. Overall, these results suggest that early intervention with C. butyricum could have positive effects on Muscovy ducks’ intestinal health, which might be attributed to the modulation in the intestinal microbial composition and the increased concentrations of short-chain fatty acids (SCFAs). C. butyricum might even have the potential to help the colonization of beneficial bacteria in the intestine microbiota in Muscovy ducks in poultry and other livestock.

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