Conversion of waste cooking oil by rhodococcal lipase immobilized in gellan gum

Recently, the application of lipase enzyme as biocatalyst in the conversion of waste cooking oil (WCO) to free fatty acids and glycerol has been trending well. Therefore, the present study attempts to use WCO which is found in abundance in Malaysia as the substrate for halal microbial lipase conversion to glycerol which can be exploited in the food industry. The workability of free lipase for WCO conversion, however suffers severely due to potential denaturation of the enzyme and extended reaction time. Thus, this study embraced the immobilization method to encapsulate crude lipase extracted from Rhodococcus pyridinivorans strain UCC 0009 in gellan gum and calcium alginate, respectively and compared their ability for WCO conversion to free crude lipase. The gellan gum and calcium alginate-immobilized crude lipase evidently exhibited greater WCO conversion, demonstrating 2.18-fold and 1.61-fold enhanced lipase activity, respectively in comparison to free crude lipase. The repeated reuse of the gellan gum-immobilized crude lipase maintained reasonable lipase activity for 9 cycles, retaining an average 85 % WCO conversion for the first seven cycles and 67 % conversion in the subsequent batches. Thus, the immobilized halal lipase can be foreseen as a green substitute to chemical catalyst for WCO conversion which meets the worldwide demand for clean technologies.

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Biodiesel production from waste cooking oil using a novel biocatalyst of lipase enzyme immobilized magnetic nanocomposite

Fuel ◽

10.1016/j.fuel.2021.123057 ◽

2022 ◽

Vol 313 ◽

pp. 123057

Author(s):

Ehsan Parandi ◽

Maryam Safaripour ◽

Magda H. Abdellattif ◽

Majid Saidi ◽

Alireza Bozorgian ◽

...

Keyword(s):

Waste Cooking Oil ◽

Magnetic Nanocomposite ◽

Biodiesel Production ◽

Cooking Oil ◽

Lipase Enzyme

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Application of Immobilized Lipase Enzyme for the Production of Biodiesel from Waste Cooking Oil

Asian Journal of Biological Sciences ◽

10.3923/ajbs.2013.322.330 ◽

2013 ◽

Vol 6 (7) ◽

pp. 322-330 ◽

Cited By ~ 2

Author(s):

Srinidhi Desikan ◽

Rekha Kannan ◽

Krishnan Narayanan ◽

D.J. Mukesh Kumar ◽

P.T. Kalaichelv

Keyword(s):

Immobilized Lipase ◽

Waste Cooking Oil ◽

Cooking Oil ◽

Lipase Enzyme

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“Statistical Optimization of Bio Diesel Production from Waste Cooking Oil Using Pva -Alginate Immobilized Lipase Enzyme as Bio Catalyst”

SSRN Electronic Journal ◽

10.2139/ssrn.3598085 ◽

2020 ◽

Author(s):

Veeranna S. Hombalimath ◽

Shameen Sultana M. Sultana ◽

Sharanappa A. A. A ◽

Anil R. Shet R. Shet ◽

Laxmikant. R. patil R. Patil ◽

...

Keyword(s):

Immobilized Lipase ◽

Waste Cooking Oil ◽

Statistical Optimization ◽

Cooking Oil ◽

Lipase Enzyme ◽

Diesel Production

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The Kinetics of Interesterfication on Waste Cooking Oil (Sunflower Oil) for the Production of Fatty Acid Alkyl Esters using a Whole Cell Biocatalyst (Rhizopus oryzae) and Pure Lipase Enzyme

International Journal of Green Energy ◽

10.1080/15435075.2014.882339 ◽

2014 ◽

Vol 12 (10) ◽

pp. 1012-1017 ◽

Cited By ~ 5

Author(s):

B. Bharathiraja ◽

J. Jayamuthunagai ◽

R. Praveenkumar ◽

M. Jayakumar ◽

S. Palani

Keyword(s):

Fatty Acid ◽

Sunflower Oil ◽

Rhizopus Oryzae ◽

Waste Cooking Oil ◽

Alkyl Esters ◽

Cooking Oil ◽

Lipase Enzyme ◽

Whole Cell Biocatalyst ◽

Oil Sunflower ◽

Kinetics Of

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Production of Biodiesel from High Acid Value Waste Cooking Oil Using an Optimized Lipase Enzyme/Acid-Catalyzed Hybrid Process

E-Journal of Chemistry ◽

10.1155/2009/801756 ◽

2009 ◽

Vol 6 (s1) ◽

pp. S485-S495 ◽

Cited By ~ 14

Author(s):

N. Saifuddin ◽

A. Z. Raziah ◽

H. Nor Farah

Keyword(s):

Acid Value ◽

Reaction Rates ◽

Waste Cooking Oil ◽

Biodiesel Production ◽

Hybrid Process ◽

Initial Reaction ◽

Cooking Oil ◽

High Acid ◽

Lipase Enzyme ◽

Acid Catalyzed

The present study is aimed at developing an enzymatic/acid-catalyzed hybrid process for biodiesel production using waste cooking oil with high acid value (poor quality) as feedstock. Tuned enzyme was prepared using a rapid drying technique of microwave dehydration (time required around 15 minutes). Further enhancement was achieved by three phase partitioning (TPP) method. The results on the lipase enzyme which was subjected to pH tuning and TPP, indicated remarkable increase in the initial rate of transesterification by 3.8 times. Microwave irradiation was found to increase the initial reaction rates by further 1.6 times, hence giving a combined increase in activity of about 5.4 times. The optimized enzyme was used for hydrolysis and 88% of the oil taken initially was hydrolyzed by the lipase. The hydrolysate was further used in acid-catalyzed esterification for biodiesel production. By using a feedstock to methanol molar ratio of 1:15 and a sulphuric acid concentration of 2.5%, a biodiesel conversion of 88% was obtained at 50 °C for an hour reaction time. This hybrid process may open a way for biodiesel production using unrefined and used oil with high acid value as feedstock.

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Enzymatic Production of Bio-Diesel from Waste Cooking Oil Using Lipase

The Open Chemical Engineering Journal ◽

10.2174/1874123100802010084 ◽

2008 ◽

Vol 2 (1) ◽

pp. 84-88 ◽

Cited By ~ 7

Author(s):

Sulaiman Al-Zuhair

Keyword(s):

Immobilized Lipase ◽

Sol Gel ◽

Waste Cooking Oil ◽

Molar Ratio ◽

Free Form ◽

Kinetics Parameters ◽

Enzymatic Production ◽

Cooking Oil ◽

Free Lipase

The applications of lipase immobilized on ceramic beads and entrapped in sol-gel matrix, in the production of bio-diesel from waste cooking oil, are compared to that of free lipase. Experimental determination of the effect of molar equivalent of methanol, to moles of ester bond in the triglyceride, on the rate of the enzymatic trans-esterification was experimentally determined. It was found that for the same weight of lipase used, the production of bio-diesel was much higher using lipase immobilized on ceramic beads in comparison to that using lipase entrapped in sol-gel and in free form. Substrates inhibition effect was observed in all cases, which agrees with previous results found in literature. The optimum methanol:oil molar ratio was found to be 0.87 for immobilized lipase from yeast source, C. antartica and 1.00 for free lipase from the same yeast source and immobilized lipase from bacterial source, P. cepacia. On the other hand, it was shown that biodieasel can be produced in considerable amounts, with yield reaching 40%, in absence of organic solvent using immobilized lipase, from P. cepacia, on ceramic beads. The results of this study can be used to determine the kinetics parameters of mathematical models which describe the system.

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Conversion of Waste Cooking Oil to Glycerol by Halal Microbial Lipase

IOP Conference Series Earth and Environmental Science ◽

10.1088/1755-1315/505/1/012056 ◽

2020 ◽

Vol 505 ◽

pp. 012056

Author(s):

N M Maegala ◽

S Anupriya ◽

A Hazeeq Hazwan ◽

Y Nor Suhaila ◽

A Hasdianty

Keyword(s):

Waste Cooking Oil ◽

Microbial Lipase ◽

Cooking Oil

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Fe3O4-PDA-Lipase as Surface Functionalized Nano Biocatalyst for the Production of Biodiesel Using Waste Cooking Oil as Feedstock: Characterization and Process Optimization

Energies ◽

10.3390/en13010177 ◽

2019 ◽

Vol 13 (1) ◽

pp. 177 ◽

Cited By ~ 12

Author(s):

Tooba Touqeer ◽

Muhammad Waseem Mumtaz ◽

Hamid Mukhtar ◽

Ahmad Irfan ◽

Sadia Akram ◽

...

Keyword(s):

Iron Oxide ◽

Residual Activity ◽

Waste Cooking Oil ◽

Maximum Activity ◽

Mass Spectrometry Analysis ◽

Gas Chromatography Mass Spectrometry ◽

Thermal Method ◽

Enzymatic Transesterification ◽

Cooking Oil ◽

Free Lipase

Synthesis of surface modified/multi-functional nanoparticles has become a vital research area of material science. In the present work, iron oxide (Fe3O4) nanoparticles prepared by solvo-thermal method were functionalized by polydopamine. The catechol groups of polydopamine at the surface of nanoparticles provided the sites for the attachment of Aspergillus terreus AH-F2 lipase through adsorption, Schiff base and Michael addition mechanisms. The strategy was revealed to be facile and efficacious, as lipase immobilized on magnetic nanoparticles grant the edge of ease in recovery with utilizing external magnet and reusability of lipase. Maximum activity of free lipase was estimated to be 18.32 U/mg/min while activity of Fe3O4-PDA-Lipase was 17.82 U/mg/min (showing 97.27% residual activity). The lipase immobilized on polydopamine coated iron oxide (Fe3O4_PDA_Lipase) revealed better adoptability towards higher levels of temperature/pH comparative to free lipase. The synthesized (Fe3O4_PDA_Lipase) catalyst was employed for the preparation of biodiesel from waste cooking oil by enzymatic transesterification. Five factors response surface methodology was adopted for optimizing reaction conditions. The highest yield of biodiesel (92%) was achieved at 10% Fe3O4_PDA_Lipase percentage concentration, 6:1 CH3OH to oil ratio, 37 °C temperature, 0.6% water content and 30 h of reaction time. The Fe3O4-PDA-Lipase activity was not very affected after first four cycles and retained 25.79% of its initial activity after seven cycles. The nanoparticles were characterized by FTIR (Fourier transfer infrared) Spectroscopy, XRD (X-ray diffraction) and TEM (transmission electron microscopy), grafting of polydopamine on nanoparticles was confirmed by FTIR and formation of biodiesel was evaluated by FTIR and GC-MS (gas chromatography-mass spectrometry) analysis.

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