Isolation and identification of bacteria from four important poplar pests

2017 ◽  
Vol 43 (1) ◽  
pp. 34 ◽  
Author(s):  
Mustafa Yaman ◽  
Ömer Ertürk ◽  
Sabri Ünal ◽  
Fazil Selek
Keyword(s):  
Urban Areas ◽  
Bacterial Flora ◽  
Bacterial Identification ◽  
Bacterial Isolates ◽  
Isolation And Identification ◽  
Disease Symptoms ◽  
Ecological Control ◽  
Identification Of Bacteria ◽  
First Time ◽  
Chemical Pesticides

In this study, the bacterial flora of important poplar pests was studied. This included Cryptorhynchus lapathi (Coleoptera: Curculionidae), Sciapteron tabaniformis (Lepidoptera: Sesiidae), Nycteola asiatica (Lepidoptera: Nolidae) and Gypsonoma dealbana (Lepidoptera: Tortricidae). The final goal was to propose alternative ecological control agents for poplar pests and decrease the undesirable effects caused by chemical pesticides in urban areas and urban forests. Forty-three bacteria were isolated from the larvae and adults exhibiting characteristic disease symptoms of these pests in five different localities for the first time. All bacterial isolates were cultured and identified using VITEK bacterial identification systems (VITEK® 2 GN ID card prod. no; 21341 and VITEK® 2 GP ID card prod. no; 21342, bioMerieux, Marcy l’Etoile). The members of the genera from Bacillaceae and Enterobacteriaceae families were most commonly isolated from both pest insects.

scholarly journals Isolation and identification of bacteria and parasites in glass eel (Anguilla spp.)

2021 ◽  
Vol 322 ◽  
pp. 02012
Author(s):  
Septyan Andriyanto ◽  
Hessy Novita ◽  
Tuti Sumiati ◽  
Taukhid
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Prevalence Rate ◽  
Bacterial Species ◽  
Bacterial Identification ◽  
Biochemical Method ◽  
Isolation And Identification ◽  
Glass Eel ◽  
Pathogenic Agent ◽  
Identification Of Bacteria ◽  
Glass Eels

The disease is the main agent that causes mortality of fish, especially during seed stages. The research aimed to find out bacteria and parasitic speciesin glass eel, Anguilla spp. Bacterial identification was carried out by a biochemical method. The prevalence of bacterial species was calculated using the El-Gohary et al. (2020) formula, while the results of bacterial identification from glass eel were Aeromonas spp., Vibrio spp., Enterococcus spp., Staphylococcus spp., Planococcus spp., Lactobacillus spp., Listeria spp., Citrbacterfreundii, Neisseria spp., Pseudomonas aeruginosa, Kurthia spp., Streptococcus spp., and Corynebacterium spp. It was found that the five highest prevalence rate was for Listeria spp. (39.64%), followed by Aeromonas spp. (26.13%), Staphylococcus spp. (16.22%), Corynebacterium spp. (5.41%), Lactobacillus spp. (2.70%), and the lowest prevalence rate was Streptococcus spp. (0.90%). The type of parasitic pathogen obtained was Trichodina spp. (2,70%), Dactylogyrus spp. (2,70%) and Gyrodactylus spp. (2,70%). Bacterial and parasites identified in glass eels need further verification on the epizootiology characteristic of each pathogenic agent.

scholarly journals Identification of Bacteria in the Sputum of a Cystic Fibrosis patient; A Comparison of Phenotypic and Molecular Methods

2017 ◽  
Vol 11 (1) ◽  
pp. 384-386 ◽  
Author(s):  
Mubarak Alfaresi ◽  
Bassam Mahboub
Keyword(s):  
Cystic Fibrosis ◽  
Bacterial Species ◽  
Transmembrane Conductance Regulator ◽  
Bacterial Identification ◽  
Molecular Method ◽  
Isolation And Identification ◽  
Pcr Product ◽  
Clinical Culture ◽  
Identification Of Bacteria ◽  
Cf Transmembrane Conductance Regulator

Background: Cystic fibrosis (CF), caused by mutations in the CF transmembrane conductance regulator gene, is a common autosomal recessive disease. Accurate isolation and identification of the bacteria underlying these infections are is critical to the therapeutic management of CF. Objective: To compare phenotypic bacterial identification with a molecular method in a CF patient sputum. Methods: Bacterial identification done by standard microbiological method from a CF patient. Same sample underwent a molecular method involving 16S rDNA amplification, cloning, and sequencing. Results: All isolated bacteria from culture were also found after cloning PCR Product. Conversely, 9 pathogenic bacterial species were only detected after PCR and cloning. Conclusion: This study supports prior suggestions that a sequence-based molecular approach to clinical microbiology can significantly enhance the standard clinical culture-based view.

Isolation and Identification of Bacteria from Marine Biofilms

2005 ◽  
Vol 277-279 ◽  
pp. 612-617 ◽  
Author(s):  
Yoo Kyung Lee ◽  
Kae Kyoung Kwon ◽  
Kyeung Hee Cho ◽  
Jae Hyun Park ◽  
Hong Kum Lee
Keyword(s):  
Culturable Bacteria ◽  
Bacterial Isolates ◽  
Isolation And Identification ◽  
Gram Positive ◽  
Gram Positive Bacteria ◽  
Rdna Sequences ◽  
Marine Biofilms ◽  
Antifouling Agent ◽  
Identification Of Bacteria ◽  
Invertebrate Larvae

In the marine environment, biofilms cover most of the subtidal and intertidal solid surfaces. Culturable bacteria forming marine biofilms were isolated on artificial substrate called acrylic coupons. The bacterial isolates were identified through a comparison of 16S rDNA sequences. A total of 115 strains were cultured and identified, 45 of which showed the same sequences with other strains. Therefore, 70 strains were finally identified. The bacterial isolates belonged to a–Proteobacteria (32 isolates), g–Proteobacteria (12 isolates), CFB group bacteria (4 isolates), high GC Gram-positive bacteria (9 isolates), and low GC Gram-positive bacteria (13 isolates). The bacterial isolates may be used as standard bacteria to test new antifouling agent. They may also be utilized as useful bacteria to enhance the settlement of commercial algae and invertebrate larvae for aquaculture.

scholarly journals Isolasi Dan Identifikasi Bakteri Yang Terdapat Pada Saluran Pencernaan Ikan Gurami (Osphronemus gouramy) Dengan Pemberian Pakan Komersil Yang Berbeda [Isolation And Identification Of Bacterial In The Digestive Organ Of Gurami Fish (Osphronemus gouramy) With Different Commercial Feed]

2019 ◽  
Vol 6 (1) ◽  
pp. 87
Author(s):  
Franch Dalahi, Sri Subekti, Agustono
Keyword(s):  
Digestive Tract ◽  
Eating Habits ◽  
Bacterial Flora ◽  
Natural Food ◽  
Pseudomonas Sp ◽  
Isolation And Identification ◽  
Growth And Survival ◽  
Commercial Feed ◽  
Different Types ◽  
Identification Of Bacteria

Abstract Based on the food habits, gurami is omnivorous fish that feed herbivorous carp tend consists of natural food and artificial feed (pellets). The feed is needed to support the growth and survival of Gurami. If feed is given in accordance with the eating habits of Gurami and contain high nutritional Gurami can then growth accelerated faster. Some types of bacteria found in the digestive tract of animals has an important role in order to improve feed utilization, fish health, and environmental quality improvement and microorganisms. In addition, some bacterial flora in the gastrointestinal tract plays an important role and produce several types of enzymes in the digestive tract may contribute to the metabolism of the host. The purpose of this study was to determine the bacteria in the digestive tract of Gurami (Osphronemus gouramy) were given three different types of commercial feed. This study begins with a sampling of Gurami (Oshpronemus gouramy) of maintenance in the Faculty of Fisheries and Marine at Airlangga University in Surabaya and then do stage isolation and identification of bacteria in laboratory bacteria and Microbiology, Faculty of Veterinary Medicine, Airlangga University Surabaya. This study was conducted in July 2013. This research is a descriptive study, the method of solving the problem by describing the subject matter into a form that is easy to understand and based on facts. Results of research on gurami fish that given by three different types of commercial feed, there are two types of bacteria that dominate in the digestive tract of carp, the Pseudomonas sp. and Serratia sp. Both types of bacteria has the potential to help the digestive system of gurami fish. The result of this research are need for further research using the bacterium Pseudomonas sp. and Serratia sp. as probiotics.

scholarly journals Isolation and identification of bacteria from tracheas and lungs of buffaloes in Dinajpur

2013 ◽  
Vol 2 (1) ◽  
pp. 31-33 ◽  
Author(s):  
Md Zulfekar Ali ◽  
Shirin Sultana
Keyword(s):  
Lung Diseases ◽  
Bacterial Isolate ◽  
Pasteurella Haemolytica ◽  
Bacterial Isolates ◽  
Isolation And Identification ◽  
Different Types ◽  
Identification Of Bacteria ◽  
Ferment Lactose ◽  
Staphylococcus Spp ◽  
And Control

The aim of the present study was the isolation and identification of bacteria from trachea and lung of slaughtered buffaloes. A total of 160 swab samples were collected from tracheas (80) and lungs (80) freshly slaughtered buffeloes from the slaughter houses of Dinajpur Sadar, Dinajpur, Bangladesh, during the period from June 2011 to February 2012. Among them, 9 tracheas (out of 80 samples) and 30 lungs (out of 80 samples) showed visible lesions. Bacterial isolates were identified based on their growth, morphological and biochemical characteristics. The most frequent (5%) bacterial isolate in tracheas was Staphylococcus spp. Pasteurella haemolytica and Staphylococcus spp. were isolated from different types of lung lesions in a frequency of 5% and 16.25%, respectively. P. haemolytica showed ?- haemolysis on blood agar and did not ferment lactose and dulcitol whereas dextrose, manitol and maltose were fermented with the production of acid. This investigation would be helpful for veterinarian for the detection, treatment and control of tracheal and lung diseases of buffaloes. DOI: http://dx.doi.org/10.3329/sjm.v2i1.15211 Stamford Journal of Microbiology, Vol.2(1) 2012: 31-33

scholarly journals Isolation and identification of bacterial flora from respiratory tract of healthy horses

2016 ◽  
Vol 13 (2) ◽  
pp. 239-246
Author(s):  
ZB Muktha ◽  
SML Kabir ◽  
MT Rahman
Keyword(s):  
Staphylococcus Aureus ◽  
Respiratory Tract ◽  
Bacterial Species ◽  
Bacterial Flora ◽  
Antibiotic Sensitivity ◽  
Aerobic Condition ◽  
Diffusion Method ◽  
Bacterial Isolates ◽  
Isolation And Identification ◽  
E Coli

This study was carried out during the period of July to December, 2014 in order to isolate and characterize bacterial flora present in the respiratory tract of healthy horses in and around BAU campus. Eighteen apparently healthy horses were used for the study. Swab samples were collected from the nasal cavity. The bacteria was isolated ,identified and characterized by cultural (aerobic condition),staining, biochemical and PCR technique. Each of the samples collected yielded at least one bacterial species. A total of 27 bacteria were isolated from the selected animals. The majority of the isolates (15/27, 55.56%) were Gram-positive and the rest (12/27, 44.44%) were Gram-negative. Bacterial isolates were Staphylococcus aureus (83.33%) and E. coli (66.66%).The antimicrobial susceptibility pattern of bacterial isolates was investigated by disc diffusion method. The antibiotic sensitivity test of Staphylococcus aureus revealed that the isolates were highly sensitive to ciprofloxacin, moderately sensitive to gentamicin and resistance to amoxicilin, ampicilin and erythromycin. On the other hand, E. coli showed moderately sensitive to ciprofloxacin and gentamycin, mildly sensitive to erythromycin and resistance to amoxicilin and ampicilin. Detection of E. coli and S. aureus from the respiratory tract of healthy horses were not unexpected. Ciprofloxacin and gentamicin could be used for therapeutic purpose, if diseases occur by these organisms in horses.J. Bangladesh Agril. Univ. 13(2): 239-246, December 2015

scholarly journals Isolation and Identification of Cellulolytic and Lignolytic Bacteria from the Gut Oryctes rhinoceros L. Larvae Decomposition of Oil Palm Empty Fruit Bunches

2018 ◽  
Vol 1 (2) ◽  
pp. 193-203 ◽  
Author(s):  
Isna Rahma Dini ◽  
Wawan Wawan ◽  
Hapsoh Hapsoh ◽  
Sriwahyuni Sriwahyuni
Keyword(s):  
Oil Palm ◽  
Bacterial Isolates ◽  
Cellulolytic Bacteria ◽  
Genus Bacillus ◽  
Isolation And Identification ◽  
Oryctes Rhinoceros ◽  
Empty Fruit Bunches ◽  
Qualitative Test ◽  
Identification Of Bacteria ◽  
Physiological Tests

One of the organisms that helps in decomposition of oil palm empty fruit bunches is Oryctes rhinoceros L. larvae. This is because of in the gut of the larvae there are many cellulolytic bacteria and lignoliytic. The process of accelerating the decomposition of oil palm empty fruit bunches into compost can be done by optimizing the work of the bacteria. The aim of this research is to obtain cellulolytic and lignolytic bacteria from larvae O. rhinoceros L.. The research succeeded to isolate 24 isolates of cellulolytic bacteria and lignolytic bacterial isolates from the gut of O. rhinoceros larvae. Based on qualitative test, 9 isolate bacteria produced cellulolytic index above 2. The highest cellulolytic index was generated by ORL19 isolate, while the lignolytic index obtained ORL6 isolate. Based on macroscopic and microscopic identification of bacteria, biochemical and physiological tests,  it was found ORL 6 belongs to the genus Bacillus sp. while the ORL 19 belongs to the genus Citrobacter sp..

scholarly journals Mammalian Arginase Inhibitory Activity of Methanolic Extracts and Isolated Compounds from Cyperus Species

Molecules ◽  
2021 ◽  
Vol 26 (6) ◽  
pp. 1694
Author(s):  
Kamel Arraki ◽  
Perle Totoson ◽  
Alain Decendit ◽  
Andy Zedet ◽  
Justine Maroilley ◽  
...  
Keyword(s):  
Inhibitory Activity ◽  
Methanolic Extract ◽  
Significant Inhibition ◽  
Isolation And Identification ◽  
Vasorelaxant Effect ◽  
Methanolic Extracts ◽  
Phytochemical Investigation ◽  
Ic50 Values ◽  
First Time

Polyphenolic enriched extracts from two species of Cyperus, Cyperus glomeratus and Cyperus thunbergii, possess mammalian arginase inhibitory capacities, with the percentage inhibition ranging from 80% to 95% at 100 µg/mL and 40% to 64% at 10 µg/mL. Phytochemical investigation of these species led to the isolation and identification of two new natural stilbene oligomers named thunbergin A-B (1–2), together with three other stilbenes, trans-resveratrol (3), trans-scirpusin A (4), trans-cyperusphenol A (6), and two flavonoids, aureusidin (5) and luteolin (7), which were isolated for the first time from C.thunbergii and C. glomeratus. Structures were established on the basis of the spectroscopic data from MS and NMR experiments. The arginase inhibitory activity of compounds 1–7 was evaluated through an in vitro arginase inhibitory assay using purified liver bovine arginase. As a result, five compounds (1, 4–7) showed significant inhibition of arginase, with IC50 values between 17.6 and 60.6 µM, in the range of those of the natural arginase inhibitor piceatannol (12.6 µM). In addition, methanolic extract from Cyperus thunbergii exhibited an endothelium and NO-dependent vasorelaxant effect on thoracic aortic rings from rats and improved endothelial dysfunction in an adjuvant-induced arthritis rat model.

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