scholarly journals The potency of spice extracts in fish skin collagen production

2021 ◽  
Vol 322 ◽  
pp. 04004
Author(s):  
Nina Hermayani Sadi ◽  
Visal Khatami ◽  
Ade Heri Mulyati
Keyword(s):  
Total Protein ◽  
Total Protein Content ◽  
Fishy Odor ◽  
Collagen Production ◽  
Liquid Extract ◽  
Acid Soluble Collagen ◽  
Skin Collagen ◽  
Significant Difference ◽  
Fish Collagen ◽  
Total Fat

Spices can be used as an alternative washing agent in fish collagen production, replacing organic materials' commonly used washing agent. Many Asians, including Indonesian traditional culinary use spices to remove the fishy odor. Therefore this research was conducted to obtain a suitable spice used as a washing agent in fish collagen production. Ginger, galangal, lemongrass, bay leaves, lime leaves, and pandan leaves were used as the tested spices in the form of 5% liquid extract in water. The ANOVA and LSD test on fishy odor test data showed that most respondents preferred Pangasius skin soaked in galangal liquid extract and had no significant difference in odor compared to the control (ethanol). Pangasius skin soaked in galangal extract had the total protein and ash content lower than control, while the total fat and water content were higher. Soaking in galangal extract also made Pangasius skin have a higher degree in swelling and total protein content in its acid-soluble collagen (ASC) extract than in control. The higher value of the two latter parameters was also found in the descaling Tuna skin soaked in galangal extract.

scholarly journals FATTENING AND MEAT QUALITIES OF GEESE OF OBROSHIN SELECTION WITH GRAY AND WHITE PLUMAGE

2021 ◽  
Vol 22 (1) ◽  
pp. 243-250
Author(s):  
L. V. Ferenc ◽  
M. D. Petriv
Keyword(s):  
Total Protein ◽  
Research Work ◽  
Live Weight ◽  
Total Protein Content ◽  
Mass Selection ◽  
Group I ◽  
Breed Group ◽  
Significant Difference ◽  
Males And Females ◽  
Group Ii

The research work provided a solution to the problem of preserving the gene pool of Obroshyn gray (OS) and Obroshyn white (OB) geese, which will improve and enhance the valuable qualities of the original flock of geese, gain the desired economic characteristics and thus ensure the competitiveness of the industry in modern conditions. The research was conducted in the laboratory of small livestock ISGKR NAAS and on the basis of SE DG "Miklashiv". The main method of breeding is the selection and selection of individuals with high productive qualities in order to obtain the same type of bird that would meet the planned performance parameters. Selection work was carried out by individual mass selection, aimed at consolidating the standard for each breed group of traits. Before the beginning of the breeding period, males and females of both groups were individually assessed by the exterior, typical plumage, live weight. It was found that both Obroshyn gray and Obroshyn white geese were well selected for productive characteristics and showed high fattening and meat qualities. All birds are well adapted to the natural conditions of the western region. Body sex measurements (chest circumference, torso length, keel, and metatarsus) were higher in males of the Obroshyn gray breed group of geese. Males at different ages had higher rates than females. Obroshyn gray geese (group I) outperformed their peers from group II in terms of meat qualities, the weight of uncorked and gutted carcass, and yield of edible parts. The pre-slaughter live weight of carcasses of males of group I (OS) was 4722 g and prevailed over peers of group II (OB) by 2.94%, and the live weight of females was 4149 g and was higher by 4.19%. It was found that the difference in the number of erythrocytes in males and females was insignificant and almost does not change with age, and hemoglobin on the contrary - increases with age. Males in all study periods had higher serum total protein compared to females. The lowest rate was in males and females аt 4 weeks of age, however, there was no statistically significant difference between the groups in the number of erythrocytes, hemoglobin, and total protein content.

scholarly journals The correlation between total protein content and antioxidant activity of collagen isolated from a marine sponge Stylissa flabelliformis collected from North Lombok Indonesia coast

2021 ◽  
Vol 913 (1) ◽  
pp. 012103
Author(s):  
A L Sunarwidhi ◽  
A Rosyantari ◽  
E S Prasedya ◽  
N Ardiana ◽  
B T K Ilhami ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Protein Content ◽  
Marine Sponge ◽  
Total Protein ◽  
Total Yield ◽  
Marine Sponges ◽  
Activity Measurement ◽  
Total Protein Content ◽  
Dpph Assay ◽  
Skin Collagen

Abstract Abstract.Collagen is a fibrous protein that has recently gained high attention from the pharmaceutical industry due to its benefits on the skin. Collagen can be isolated from various resources including marine sponges. Marine sponges are found in a large amount in Indonesia and has not been widely explored for its pharmacology benefits. Here we isolate collagen from a marine sponge Stylissa flabelliformis found in North Lombok Indonesia coast. The isolation of collagen was performed followed by total protein content analysis using modified Bradford protein assay and antioxidant activity measurement using 2,2-diphenyl-1-picrylhydrazyl(DPPH) assay. The total yield of the collagen isolate obtained was 3.5% and it had a total of 0.755mg/ml protein. DPPH assay has shown that the collagen isolate had antioxidant activity with an IC50 of 61.5±2.132 ppm. Based on Spearman correlation assay, the antioxidant activity was found to be correlated with the protein content of the isolate (r value=0.8). These results show the potency of using the collagen isolated from marine sponge Stylissa flabelliformis for further antioxidant benefits.

scholarly journals Investigating chemical parameters in hot dog sausages from Novi Sad market

2011 ◽  
Vol 65 (5-6) ◽  
pp. 385-397
Author(s):  
Nadezda Prica ◽  
Milan Baltic ◽  
Milica Zivkov-Balos ◽  
Zeljko Cupic ◽  
Zeljko Mihaljev
Keyword(s):  
Protein Content ◽  
Total Protein ◽  
Fat Content ◽  
Total Protein Content ◽  
Average Content ◽  
Chemical Parameters ◽  
Significant Difference ◽  
The Difference ◽  
Novi Sad ◽  
Hot Dog

Meat products play an important role in the modern diet. The production of cooked sausages comprises an important segment in the complex meat industry. Hot dogs are prepared according to production specifications and differences in the quality of products can therefore be expected. This paper presents a comparative analysis of chemical parameters in products of five different manufacturers at the Novi Sad market. Water content, fat, protein, sodium chloride, the relative protein content of connective tissue in meat protein (RCP) and ash were analyzed. The difference between the average contents of chemical quality parameters in hot dogs was examined. The average water content ranged from 56.29?3.30% to 61.44?4.08%. The average total fat content ranged from 17.60?3.07% to 20.66?4.65%. No statistically significant difference (p?0.05) was detected in the fat content of the products of manufacturers D and B, as well as C and B. Between the average fat content of the producers E and D the difference in fat content was found to be p?0.01. In all other cases, the difference between the average fat content was p?0.001. The average total protein content in hot dogs ranged from 11.77?1.08% and 15.41?1.82. No statistically significant difference (p?0.05) was detected between the average total protein content in hot dogs of manufacturers A and C. Between the average protein content of D and E meat manufacturers the statistically significant difference had a value of p?0.01, and the average protein content in hot dogs of all other manufacturers had a significant difference (p?0.001). The total protein content in all five manufacturers of hot dogs met the prescribed standard (10%). RCP content in the hot dogs of different producers ranged from 17.34?0.95% to 22.33?2.08%. No statistically significant difference (p?0.05) was detected in the average contents of RCP in hot dogs of manufacturers D and E and A and B. but a statistically significant difference was detected (p?0.001). between the average content of RCP in all other manufacturers of hot dogs. The average chloride content in hot dogs from different manufacturers ranged from 1.30?0.10% to 2.55%?0.16%. The average ash content in different hot dog producers ranged from 1.99?0.18% to 3.46?0.22%. The average content of chloride and ash in all five manufacturers was significantly different.

scholarly journals The content of total protein and protein fractions in cows during pregnancy and their diagnostic value

2020 ◽  
Vol 22 (99) ◽  
pp. 198-202
Author(s):  
Ya. S. Stravsky ◽  
N. P. Boltyk ◽  
R. M. Sachuk ◽  
V. I. Serheyev ◽  
T. M. Rushchynska
Keyword(s):  
Total Protein ◽  
Postpartum Period ◽  
Diagnostic Value ◽  
Protein Fractions ◽  
Total Protein Content ◽  
The Third ◽  
Significant Difference ◽  
Albumin Content ◽  
Consistent Increase ◽  
Two Peaks

The aim of the article was to study the content of total protein and protein fractions in the blood of cows during pregnancy and determine their diagnostic value. The studies were performed as follows. Within 7 days after insemination on the principle of analogues selected a group of 100 cows. Pregnancy was diagnosed by rectal examination of cows 60 days after insemination. In experimental animals, starting from the 25th to the 29th day after insemination, blood samples for morphological and biochemical studies were taken monthly before delivery. The total protein content in the serum was determined by the biuret reaction, protein fractions – nephelometric method. Depending on the course of labor and the postpartum period, cows were divided into two groups. The first group included 30 animals with a physiological course of the postpartum period. The second group was formed of 30 cows with subinvolution of the uterus. According to this goal, the dynamics of total protein, albumin, alpha-, beta- and gammaglobulins in the blood of cows with physiological course of pregnancy and prone to the development of subinvolution of the uterus from the first to 9 months of pregnancy. It was found that in clinically healthy cows the onset of pregnancy is characterized by a high (0.89) albumin-globulin ratio, which in the fourth month of pregnancy decreases to 0.78, in the fifth or sixth months increases to 0.85, followed by a decrease to 0.78 before childbirth. In cows prone to uterine subinvolution, a significant difference in albumin content was found in the first month of pregnancy (47.07 ± 1.21 vs. 41.02 ± 1.26 %). In the third to fifth months of pregnancy, there was an increase in the proportion of albumin in the protein to 44.62 ± 0.88 % and a new decrease in the seventh to ninth months to 41.96 ± 0.3 %. In the first month of pregnancy, the albumin-globulin ratio was 0.7, with a tendency to increase in the fifth to sixth months of pregnancy to 0.8, and decrease to 0.72 before calving. Analyzing the dynamics of alpha-globulins in clinically healthy cows, we noted two peaks of decrease in their percentage - in the third month of pregnancy (from 16.02 ± 0.08 to 15.14 ± 0.06 %) and in the fifth month , 96 ± 0.48 %) and a consistent increase in the seventh to ninth months of pregnancy (up to 17.22 ± 0.13 %). And in cows prone to the development of uterine subinvolution, with a slightly higher percentage at the beginning of pregnancy (16.02 ± 0.08 to 17.08 ± 0.05 %), we did not notice significant changes in the percentage of this globulin, except for a significant increase in seventh to ninth month of pregnancy (up to 18.01 ± 0.24 – 19.01 ± 0.32 %). It is proposed to use the albumin-globulin ratio at 6, 7, 8, 9 months of gestation as a prognostic factor in the detection of cows prone to uterine subinvolution.

Cytotoxic Effects of Paracetamol in Vitro: The Effects of an S9 Fraction

1996 ◽  
Vol 24 (5) ◽  
pp. 715-725
Author(s):  
Katarina Ruppová ◽  
Darina Slameñová ◽  
Ladislave Wsølová ◽  
Alena Gábelová ◽  
Maria Vargová
Keyword(s):  
Protein Content ◽  
Hela Cells ◽  
Total Protein ◽  
Total Protein Content ◽  
Leucine Incorporation ◽  
Short Term ◽  
Cytotoxic Effects ◽  
Significant Difference ◽  
S9 Fraction

The cytotoxic effects of short-term and long-term exposure of HeLa cells to paracetamol (acetaminophen) were assayed by total protein content reduction (microprotein assay) and by [14C]-L-leucine incorporation into macromolecular acid-insoluble cell fraction. The level of total protein content was followed over 72 hours and the level of [14C]-L-leucine incorporation over 24 hours, after paracetamol treatment. Statistical evaluation did not show a significant difference between results obtained by these two methods. In addition, the influence of S9 fraction on [14C]-L-leucine incorporation and the growth activity of paracetamol-treated HeLa cells were assayed. In these experiments, short-term paracetamol treatments (2 hours in phosphate buffered saline), were used. Statistical analysis of the data did not show an increase in paracetamol-induced cytotoxicity in the presence of the S9 fraction; on the contrary, a protective effect of S9 fraction on paracetamol-treated cells was found.

scholarly journals АКТИВНІСТЬ ФЕРМЕНТІВ У ГЕПАТОПАНКРЕАСІ КОРОПА (CYPRINUS CARPIO L.) ЗА ДІЇ СУЛЬФАНІЛАМІДУ

2016 ◽  
Vol 6 (3) ◽  
pp. 377-382
Author(s):  
Y. N. Kurbatova ◽  
V. M. Mykhalskaia ◽  
L. V. Malyuga ◽  
L. V. Gayova
Keyword(s):  
Protein Content ◽  
Total Protein ◽  
Test Group ◽  
The Body ◽  
Total Protein Content ◽  
Urea Content ◽  
Low Concentrations ◽  
Significant Difference ◽  
Aquarium Water ◽  
Activity Of Enzymes

<p align="center"> </p><p>The results of studies of the effect of different concentrations of sulfonamides in the aquarium water on the activity of enzymes and protein metabolism in the hepatopancreas of carp yearlings were presented. It was found that the sulfanilamide added to the aquarium water at a concentration of 1.10; 3.15 and 6.30 mg / dm3 and 3 had no effect on the total protein content in the fish hepatopancreas during three days of exposure at first, second and third experimental groups compared with the control. It is proved that when the concentration of water in the aquarium sulfanilamide at a dose of 1.10 and 3.15 mg / dm3 in the hepatopancreas urea content compared with the control is not changed, while a dose of 6,30 mg / dm3 it had increased by 4%. Probably, such an increase in urea levels in the hepatopancreas of fish third test group associated with the activation of regulatory mechanisms in the body of carp to maintain a positive nitrogen balance and reduce the toxic effects of ammonia. We also did not reveal significant difference between the indices of glucose concentration in the hepatopancreas of carp experimental groups compared with the control. We founded that the activity of alanine aminotransferase and aspartate aminotransferase in hepatopancreas of carp experimental groups did not differ from controls, suggesting there was no effect of low concentrations of sulfonamides in the water after brief operation of the xenobiotic on the functional state of the hepatopancreas. Conducted experiments did not revealed changes in lipase activity as α-amylase and fish hepatopancreas in first, second and third experimental groups compared with the control. Their activity remained within the normal characteristic of the species and indicates that sulfanilamide virtually had no effect on the intensity of the hydrolytic processes in the intestines of fish. The performed investigations proved that carp yearlings were capable of adapting to minor concentrations of sulfonamides in water, as evidenced by its lack of effect on the total protein content, the concentration of glucose, urea and the activity of several enzymes in the hepatopancreas of fish.</p>

Seromucoid Determination: Variables and Modifications

1960 ◽  
Vol 6 (3) ◽  
pp. 189-198 ◽  
Author(s):  
T M Asher ◽  
G R Cooper
Keyword(s):  
Environmental Factors ◽  
Protein Content ◽  
Perchloric Acid ◽  
Total Protein ◽  
Standard Procedure ◽  
Total Protein Content ◽  
Inflammatory Condition ◽  
Freezing And Thawing ◽  
Significant Difference

Abstract The technic of Winzler (1) for the determination of seromucoid has been studied to measure its reproducibility and the effect of certain environmental factors. Values obtained are fairly reproducible (a difference of 45 mg./l00 ml. BSAP between two specimens indicates a significant difference at the P = 0.05 level). Variables such as temperature, freezing and thawing, speed of addition of perchloric acid, and total protein content of serum have no practical influence on the final SM values. A modification in technic involving determinations on three different dilutions of a specimen gives differences greater than those found with the standard procedure for sera from patients with an inflammatory condition.

scholarly journals Profiling of Cerebrospinal Fluid Lipids and Their Relationship with Plasma Lipids in Healthy Humans

Metabolites ◽  
2021 ◽  
Vol 11 (5) ◽  
pp. 268
Author(s):  
Kosuke Saito ◽  
Kotaro Hattori ◽  
Shinsuke Hidese ◽  
Daimei Sasayama ◽  
Tomoko Miyakawa ◽  
...  
Keyword(s):  
Cerebrospinal Fluid ◽  
Total Protein ◽  
Plasma Lipids ◽  
Plasma Lipid ◽  
Lipid Profiles ◽  
Brain Diseases ◽  
Lipid Homeostasis ◽  
Total Protein Content ◽  
Lipid Profiling ◽  
Healthy Humans

Lipidomics provides an overview of lipid profiles in biological systems. Although blood is commonly used for lipid profiling, cerebrospinal fluid (CSF) is more suitable for exploring lipid homeostasis in brain diseases. However, whether an individual’s background affects the CSF lipid profile remains unclear, and the association between CSF and plasma lipid profiles in heathy individuals has not yet been defined. Herein, lipidomics approaches were employed to analyze CSF and plasma samples obtained from 114 healthy Japanese subjects. Results showed that the global lipid profiles differed significantly between CSF and plasma, with only 13 of 114 lipids found to be significantly correlated between the two matrices. Additionally, the CSF total protein content was the primary factor associated with CSF lipids. In the CSF, the levels of major lipids, namely, phosphatidylcholines, sphingomyelins, and cholesterolesters, correlated with CSF total protein levels. These findings indicate that CSF lipidomics can be applied to explore changes in lipid homeostasis in patients with brain diseases.

Prediction of Human Acute Toxicity by the Hep G2/24-hour/Total Protein Assay, with Protein Measurement by the CBQCA Method

2005 ◽  
Vol 33 (3) ◽  
pp. 207-213 ◽  
Author(s):  
Paul J. Dierickx
Keyword(s):  
Protein Content ◽  
Total Protein ◽  
Total Protein Content ◽  
Human Toxicity ◽  
Hep G2 ◽  
Vitro Assay ◽  
Protein Assay ◽  
Lowry Method ◽  
Total Protein Assay

In our previously described Hep G2/24-hour/total protein assay, protein levels were measured by using the Lowry method. This assay was the best acute in vitro assay for the prediction of human toxicity within the Multicentre Evaluation of In Vitro Cytotoxicity (MEIC) study. In order to increase the MEIC data-base with a wider range of chemicals, we were interested in introducing the more practical 3-(4-carboxybenzoyl)-quinoline-2-carboxaldehyde (CBQCA) method for the quantification of the total protein content. Therefore, we investigated whether the same good results for the prediction of acute human toxicity would be obtained with the CBQCA method. The cells were treated for 24 hours, then cytotoxicity was determined by measuring the total protein content with CBQCA. The results were quantified by using the PI50c: the concentration (in mM) of test compound required to reduce the total protein content measured with the CBQCA-method by 50% as compared to the control cells. The results were compared with the PI50, the corresponding value when the Lowry method was used. A relatively low correlation was observed between PI50 and PI50c, reflecting the large and unexpected, differences when using the two protein assays. However, when comparing the log PI50c with the human toxicity, a correlation coefficient of r2 = 0.761 ( n = 44) was obtained for exactly the same series of MEIC chemicals. This value is clearly higher than that for the Lowry method ( r2 = 0.695). Compared to the Lowry method originally used, the Hep G2/24-hour/CBQCA total protein assay has the additional important advantage that it can be very easily adapted for large-scale analyses with robotic systems, including the on-line calculation of the results.

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