scholarly journals Senduro Goat Semen Characteristics as A Candidate for Low Temperature Storage

2021 ◽  
Vol 328 ◽  
pp. 08010
Author(s):  
Nur Ducha ◽  
Widowati Budijastuti ◽  
Dwi Anggorowati Rahayu
Keyword(s):  
Artificial Insemination ◽  
Low Temperatures ◽  
Semen Quality ◽  
Membrane Integrity ◽  
Animal Husbandry ◽  
Service Unit ◽  
Low Temperature Storage ◽  
Semen Characteristics ◽  
Artificial Vagina ◽  
Temperature Storage

The goal of this study was to investigate the reproductive profile of male Senduro Goats as superior male candidates based on data of semen quality. The experiment was carried out on ten male Senduro Goats kept at the Singosari Animal Husbandry Service Unit in East Java, Indonesia. An artificial vagina was used to collect sperm. The macroscopic and microscopic qualities of fresh Senduro Goat sperm have been observed. Color, volume, smell, pH, and viscosity were examples of macroscopic observations. Concentration, motility, viability, and membrane integrity were examples of microscopic observations. The results of macroscopic observations showed that the average of Senduro Goat semen was milky white; with volume 1,24 ± 0,22; 6.77 ± 0.05; thick semen. The results of microscopic observations showed that there was concentration 2,5 x 109 ± 0.1; mass motility +++; individual motility 77.5% ± 2.63; viability 88.98% ± 2.07; membrane integrity 89.71 ± 1.69. Based on the results of these studies, it can be concluded that the semen of Senduro Goat has good quality so it can be processed further, namely storage at low temperatures and applied to Artificial Insemination technology.

scholarly journals Evaluation of semen quality of buffalo frozen semen produced by Artificial Insemination Center

2018 ◽  
Vol 43 (1) ◽  
pp. 26 ◽  
Author(s):  
H. C. Mahendra ◽  
D. Samsudewa ◽  
Y. S. Ondho
Keyword(s):  
Artificial Insemination ◽  
Recovery Rate ◽  
Semen Quality ◽  
Membrane Integrity ◽  
Animal Husbandry ◽  
Mean Value ◽  
National Standard ◽  
Frozen Semen ◽  
Standard Values

This study aimed to evaluate and compare the quality of post thawing spermatozoa of buffalo frozen semen produced by artificial insemination centers on standard values, and proposed reference values (PRV). Materials of the research were 60 samples of straws obtained from three Artificial Insemination Center, which are each 20 straws, respectively. Parameters observed were motility, concentration, longevity, plasma membrane integrity (PMI), acrosome integrity (AIn) and recovery rate. The obtained data were tested by Z test then presented as mean ± standard deviation. The research results from three artificial insemination center showed that motility mean was 45.00±3.07%, concentration mean was 26.09±3.11 x106 cells/0.25mL, longevity mean was 10.38±0.75%/hour, PMI mean was 45.86±10.67%, AI mean was 73.99±5.28% and recovery rate mean was 64.38±5.16%. The conclusions of this research were the motility and concentration mean were higher than Indonesian National Standard (INS), longevity mean was lower than Department of Animal Husbandry, Dairying and Fisheries (DADF) standard but PMI and AIn mean were higher, the mean value of recovery rate was higher than of proposal value. 

Quality of ram spermatozoa separated with modified swim up method

2018 ◽  
Vol 33 (2) ◽  
pp. 62-70 ◽  
Author(s):  
A Hossain ◽  
MM Islam ◽  
F Naznin ◽  
RN Ferdousi ◽  
FY Bari ◽  
...  
Keyword(s):  
Semen Quality ◽  
Membrane Integrity ◽  
Quality Parameters ◽  
Fluid Medium ◽  
Normal Morphology ◽  
The Mean ◽  
Mass Activity ◽  
Fresh Semen ◽  
Artificial Vagina

Semen was collected from four rams, using artificial vagina and viability%, motility% and plasma membrane integrity% were measured. Fresh ejaculates (n = 32) were separated by modified swim-up separation using modified human tubal fluid medium. Four fractions of supernatant were collected at 15-minute intervals. The mean volume, mass activity, concentration, motility%, viability%, normal morphology and membrane integrity% (HOST +ve) of fresh semen were 1.0 ± 0.14, 4.1 ± 0.1 × 109 spermatozoa/ml, 85.0 ± 1.3, 89.4 ± 1.0, 85.5 ± 0.7, 84.7 ± 0.5 respectively. There was no significant (P>0.05) difference in fresh semen quality parameters between rams. The motility%, viability% and HOST +ve % of first, second, third and fourth fractions were 53.4 ± 0.5, 68.2 ± 0.3, 74.8 ± 0.3 and 65.5 ± 0.4; 55.5 ± 0.4, 66.2 ± 0.4, 74.5 ± 0.3 and 73.6 ± 0.3 and 66.7 ± 0.5, 66.8 ± 0.5, 65.2 ± 0.4 and 74.7 ± 0.5 respectively. The motility%, viability% and membrane integrity% of separated semen samples differed significantly (P<0.05) between four fractions. The mean motility% and viability% were significantly higher (P<0.05) in third fraction (74.8 ± 0.3%), whereas the mean HOST +ve% was significantly higher (P<0.05) in fourth fraction (74.7 ± 0.5). All quality parameters of separated spermatozoa were significantly (P<0.05) lower than that of fresh semen. The pregnancy rates were higher with fresh semen (71%) in comparison to that of separated sample (57%).Bangl. vet. 2016. Vol. 33, No. 2, 62-70

Kualitas Semen Cair Kambing Boer Berbahan Pengencer Air Kelapa Muda Varietas Viridis Setelah Simpan Dingin

2019 ◽  
Vol 6 (1) ◽  
pp. 78
Author(s):  
Muhammad Ade Salim ◽  
Muhammad Nur Ihsan ◽  
Nur Isnaini ◽  
Trinil Susilawati
Keyword(s):  
Semen Quality ◽  
Block Design ◽  
Animal Husbandry ◽  
Egg Yolk ◽  
Coconut Water ◽  
Boer Goat ◽  
Randomized Block Design ◽  
Frozen Semen ◽  
Artificial Vagina

ABSTRAKAir kelapa muda varietas viridisdapat dijadikan pengencer aletrnatif semen cair bagi program IB di daerah minim sarana semen beku. Tujuan penelitian ini untuk menguji pengaruh penggunaan air kelapa muda viridissebagai bahan pengencer terhadap kualitas semen cair kambing Boer setelah didinginkan. Dilaksanakanselama 3 bulan di Laboratorium Fakultas Peternakan UBUnit SumberSekar,Malang. Metodenya yaitu eksperimen. Semen dari  3 pejantan Boer umur 3-5 tahun, dikoleksi seminggu sekali dengan VB. Air kelapa mudaviridis umur 5-7 bulan serta tris aminomethane sebagai kontrol. Didesain menggunakan Rancangan Acak Kelompok (RAK) dengan 2 perlakuan yaitu P0 (tris aminomethane + 10% KT) dan  P1 (air kelapa muda viridis + 10% KT) masing-masing diulang 10 kali. Data dianalisis dengan analisis Ragam (Anova) dengan software Genstat 18. Variabelnya yaitu motilitas individu, viabilitas dan abnormalitas. Hasil penelitian yaitu motilitas individu pada P1bertahan sampai 4 hari (40,5± 24,3%), viabilitas terbaik sampai hari ke-5 (42±24,6%), abnormalitas terendah di hari ke-7(1,31± 0,6). Kesimpulannya, Pengencer air kelapa muda viridis dapat mempertahankan kualitas semen cair kambing Boer selama 4 hari untuk motilitas dan 5 hari untuk viabilitas.Kata Kunci:pengencer, air kelapa, varietas viridisABSTRACTYoung viridis coconut water could be used as an alternative to liquid semen diluent for artificial insemination program in the area with limited facility for frozen semen production. This study evaluated the use of young coconut water as a diluent on liquid semen quality of Boer goat after cold storage. This study was carried out for 3 months at Sumber Sekar Laboratory, Faculty of Animal Husbandry, University of Brawijaya, Malang. The semen was collected from 3 Boer bucks aged at 3 to 5 years old. The semen collection was done once a week with the aid of artificial vagina. The diluents used were young Viridis coconut (5 to 7 months old) and tris aminomethane. The method used was an experiment in a randomized block design with 2 treatments and 10 replicates. The treatments used were T0: tris aminomethane + 10% egg yolk (control) and T1:  young Viridis coconut water + 10% egg yolk. Data were analyzed by analysis of variance using Genstat 18 software. The variables measured were sperm individual motility, viability, and abnormality. The results showed that the sperm individual motility in T1 survived up to 4 days (40.5± 24.3%), the best viability at 5 days (42.0±24.6%),  while the lowest abnormality at 7 days (1.31±0.6). It could be concluded that: 1. Tris aminomethane diluent has higher quality with the storage length up to 9 days, 2. Young Viridis coconut water diluent could preserve liquid semen quality of Boer goat up to 4 days for sperm motility and 5 days for sperm viability.Keywords: diluents, coconut water, viridis variety

scholarly journals THE PROPERTIES OF THE CONIDIA OF STRAINS OF THE ACTINOMYCETE STREPTOMYCES LUCENSIS AND STREPTOMYCES VIOLACEUS DURING STORAGE AT LOW TEMPERATURES

Food systems ◽  
2018 ◽  
Vol 1 (3) ◽  
pp. 27-32 ◽  
Author(s):  
Natalya Yu. Sharova ◽  
Tatyana V. Vybornova ◽  
Anastasia A. Printseva ◽  
Bairta S. Manzhieva
Keyword(s):  
Low Temperature ◽  
Low Temperatures ◽  
Chloride Solution ◽  
Sodium Chloride Solution ◽  
Saline Solution ◽  
Native Solution ◽  
Aqueous Sodium Chloride Solution ◽  
Glycerin Solution ◽  
Low Temperature Storage ◽  
Temperature Storage

Collection cultures of actinomycetes are mainly stored in a dried state on adsorbents. Practiced low-temperature storage of actinomycetes at minus 70°C. The article presents the results of investigations of the properties of the conidia of strains of the actinomycete Streptomyces lucensis VKPM Ac–1743 and Streptomyces violaceus VKPM Ac–1734 is in the process of storage at minus 12°C and minus 18°C in glycerin solution and in 0,9 % aqueous sodium chloride solution. It was found that the inhibitory activity in the native solution for the studied strains stored in the glycerin solution at minus 12°C and plus 4°C, as a result of their subsequent cultivation on the starch hydrolyzate for 120 h is at the level of (450 ± 10) IE/cm3. The indicator for crops stored at minus 18°C was higher (560 ± 10) IE/cm3. Low-temperature storage of conidia in saline solution is less effective. Pigmentation is more active in the cultivation of strains stored at minus 18°C.

Effect of Storage and Temperature on Flexibility of Natural and Synthetic Rubbers

1948 ◽  
Vol 21 (4) ◽  
pp. 864-876
Author(s):  
John B. Gregory ◽  
Irving Pockel ◽  
John F. Stiff
Keyword(s):  
Low Temperature ◽  
Low Temperatures ◽  
Strain Curve ◽  
Polymer Chains ◽  
Stress Strain Curve ◽  
Low Temperature Storage ◽  
Synthetic Rubber ◽  
Loading And Unloading ◽  
Temperature Storage ◽  
Natural And Synthetic

Abstract A new method for measuring the flexibility of rubber has been described. The method consists essentially in determining the stress-strain curve obtained by loading and unloading a loop formed from a one-inch by six-inch strip cut from a test slab. A coefficient of flexibility independent of the thickness of the sample and, in addition, information on per cent resilience were obtained. By the use of the method described, the behavior of various natural and synthetic rubber gas mask facepiece compounds was studied during one month to three months' exposure at various temperatures down to −20° F. Progressive stiffening probably due to crystallization was found for natural rubber, GR-I, and GR-M compounds at low temperatures. No tendency to crystallize was noted for the GR-S compound. Of the crystallizable polymers GR-I was the most resistant, and GR-M the least resistant to stiffening during low temperature storage. It is of course evident that different polymers have inherently different degrees of resistance to low temperatures. Disregarding these inherent differences the work reported indicates that the resistance of elastomer compounds to stiffening during prolonged low temperature storage is favored by the following: 1. Use of interpolymers made from monomer mixtures having a relatively large proportion of each component, thus obtaining mutual intereference with crystallization. 2. Use of a “tight” cure which probably so impedes the movement of the polymer chains as to make crystallization difficult.

scholarly journals Biological quality of Tetrastichus howardi (Hym.: Eulophidae) reared with Tenebrio molitor (Col.: Tenebrionidae) pupae after storage at low temperatures for different periods

2018 ◽  
Author(s):  
Elison Floriano Tiago ◽  
Fabrício Fagundes Pereira ◽  
Samir Oliveira Kassab ◽  
Rogério Hidalgo Barbosa ◽  
Carlos R Garcia Cardoso ◽  
...  
Keyword(s):  
Biological Control ◽  
Low Temperatures ◽  
Tenebrio Molitor ◽  
Biological Pest Control ◽  
Control Programs ◽  
Low Temperature Storage ◽  
Large Numbers ◽  
Emergence Percentage ◽  
Temperature Storage

Introduction: The endoparasitoid Tetrastichus howardi (Olliff, 1893) (Hymenoptera: Eulophidae) can be reared with the alternative host Tenebrio molitor (Linnaeus, 1758) (Coleoptera: Tenebrionidae). Host storage at low temperatures can regulate parasitoid production and demand in biological control programs. Material and Methods: The life-cycle (egg-adult), parasitism and emergence percentage, number of parasitoids emerged per host pupae, sex ratio and longevity of the T. howardi offspring per T. molitor pupa were evaluated after low temperature storage of this host for different periods and its immature (pupae) in T. molitor pupae for five periods at 10.3 ºC. Tenebrio molitor pupae stored at 0.5 ± 0.09 °C and 2.7 ± 0.11 °C for 10 and 20 days, respectively, were adequate to produce T. howardi. Results: The biological characteristics of this parasitoid were better with T. molitor pupae stored at 0.5 ± 0.09 °C and 2.7 ± 0.11 °C for 10 and 20 days. Tetrastichus howardi immature (pupae) can be stored in T. molitor pupae for 10, 20, 30, 60 and 90 days at 10.3 ºC, preferably in pupae of this host for 10 days to produce these adults of this parasitoid for biological control programs. Discussion: These results contribute to overcoming one of the difficulties encountered in the massive production of parasitoids which is to obtain large numbers of suitable hosts when they are needed. Therefore, the possibility of conserving T. molitor pupae to rear T. howardi will be useful to use this natural enemy in biological pest control programs. Keywords: Biological control, Cold storage, Parasitoids, Progeny.

scholarly journals INCLUSION OF PROPOLIS IN RABBIT DIETS AND SEMEN CHARACTERISTICS

2015 ◽  
Vol 17 (4) ◽  
Author(s):  
Gentil Vanini de Moraes ◽  
Marcela Mataveli ◽  
Lucimar Pontara Peres de Moura ◽  
Cláudio Scapinello ◽  
Freddy Mora ◽  
...  
Keyword(s):  
Linear Models ◽  
Semen Quality ◽  
White Male ◽  
The Other ◽  
Small Reduction ◽  
Semen Volume ◽  
Semen Characteristics ◽  
Negative Effect ◽  
Different Levels ◽  
Artificial Vagina

This experiment was carried out with the objective of evaluating different levels of powder propolis in rabbit diets and their effect on semen characteristics. A total of 36 New Zealand White male rabbits were used, randomly distributed into six groups, corresponding to six propolis levels (0, 0.25, 0.50, 0.75, 1.0 and 1.25 g propolis/kg of ration). Semen was collected twice a week, using an artificial vagina. Semen volume, progressive spermatic motility, spermatic vigor, spermatic concentration and spermatic morphology were analyzed. General linear models were used for statistical analysis. The inclusion of powder propolis in the diet increased normal spermatozoa percentage and reduced spermatozoa abnormalities. The powder propolis did not affect the progressive spermatic motility, spermatic vigor or spermatic concentration. The values were considered normal for rabbits. However, a small reduction in semen volume was observed, without any negative effect on the other semen characteristics evaluated. Thus, it is possible to observe better semen quality with the inclusion of 1.25 g powder propolis/kg in the diet for reproducer rabbits.

STUDIES ON THE HOUSEFLY (MUSCA DOMESTICA L.): II. THE EFFECTS OF LOW TEMPERATURES ON LABORATORY-REARED PUPARIA

1948 ◽  
Vol 26d (1) ◽  
pp. 26-56 ◽  
Author(s):  
G. E. Bucher ◽  
A. Wilkes ◽  
J. W. MacB. Cameron
Keyword(s):  
Musca Domestica ◽  
Low Temperatures ◽  
Storage Period ◽  
Adult Emergence ◽  
Low Temperature Storage ◽  
Parental Stock ◽  
Development And Differentiation ◽  
Different Strains ◽  
Threshold Of Development ◽  
Temperature Storage

Investigations are reported on factors influencing the survival of housefly (Musca domestica L.) puparia exposed to different intensities of low temperature storage above 1 °C. Survival was decreased by lowering the temperature of storage and by increasing the duration of the storage period, or both. Mortality of adults following puparial storage was influenced by the type of rearing container used, crowding, age of puparia, humidity, concentration of gases in the pupal rearing chambers, and to some extent by changes in the food of the immature larvae but not by the age or sex ratio of the parental stock, the size of puparia, selective breeding of resistant individuals or different strains of stock. Death did not occur in cold storage but during subsequent incubation at normal temperatures and at a definite stage in development near adult emergence. Temperatures below the threshold of development caused physiological disturbances that affected the longevity, oviposition, and hatchability of eggs of the adults that survived. A proposed explanation is given of the lethal effects of low temperatures based on the interrelationships of disturbances between the relative rates of development and differentiation of various ontogenetic systems.

scholarly journals Modification of placenta blood serum proteins under low temperature effect

2013 ◽  
Vol 59 (2) ◽  
pp. 219-234
Author(s):  
O.V. Falko ◽  
N.G. Zemlianskykh ◽  
O.V. Lipina ◽  
O.S. Procopyuk
Keyword(s):  
Low Temperature ◽  
Blood Serum ◽  
Low Temperatures ◽  
Structural Changes ◽  
Serum Proteins ◽  
Protein Structures ◽  
Aggregate Formation ◽  
Low Temperature Storage ◽  
Blood Serum Proteins ◽  
Temperature Storage

Changes in environmental physical and chemical factors upon freeze-thawing and low temperature storage of biological samples can result in impairments of protein structures. This work specifies spontaneous and diamide-induced protein aggregations of placenta blood serum stored at -20° and -196°C during 2 years with SDS-PAGE. It was shown that storage of placenta blood serum at low temperatures did not cause any quantitative and qualitative changes in fraction distribution of proteins denatured with SDS in comparison to the native samples which were not frozen. Application of b-mercaptoethanol revealed that placenta blood serum proteins upon freeze-thawing did not form spontaneous aggregates linked by disulphide bridges. Oxidation of amino acid sulfhydryl groups induced by diamide and accompanied by high molecular aggregate formation proved to be a quite effective way for indirect estimation of structural changes in protein upon low temperature effects. In samples thawed after low temperature storage the protein aggregation with 4 mM diamide was significantly higher than in native serum. These discrepancies between native and frozen-thawed samples are stipulated by impairments of protein structure under low temperature and increased in accessibility of reactive SH-groups of proteins for oxidation with diamide. Structural changes in placenta blood serum proteins, which caused by low temperatures and revealed by elevated sensibility to diamide-induced aggregate formation, did not depend on temperature (-20° и -196°C) and storage terms (2 years and 3 weeks). They reflect protein reaction to freeze-thawing processes and could be sequence of ice crystal formation which takes place in unprotected media.

scholarly journals Preservation of liquid semen and Artificial Insemination in native sheep

1970 ◽  
Vol 7 (2) ◽  
pp. 305-308 ◽  
Author(s):  
S Pervage ◽  
MR Hassan ◽  
M Ershaduzzaman ◽  
MAMY Khandoker
Keyword(s):  
Sperm Motility ◽  
Artificial Insemination ◽  
Semen Quality ◽  
Sperm Concentration ◽  
Egg Yolk ◽  
Conception Rate ◽  
Abnormal Sperm ◽  
Semen Volume ◽  
Native Sheep ◽  
Artificial Vagina

An experiment was undertaken to determine the conception rate of native sheep by using Artificial Insemination with liquid ram semen. The semen was collected from ram using artificial vagina and the was stored in a refrigerator (4°C) for three days. The volume of semen was extended with egg yolk citrate diluter. A total of 63 ewes were inseminated with stored liquid semen collected from 15 rams by AV method. The total number of spermatozoa, live-dead, normal-abnormal, sperm motility and the pH was observed regularly. The average semen volume per ejaculate was 0.76-1.00ml and the sperm concentration was 2.37x109 - 4.30x109 per ejaculate. The number of normal spermatozoa and the pH was almost similar irrespective of days of storage. Number of live spermatozoa and the sperm motility were reduced with the increasing age of semen. The average conception rate (%) was obtained as 63.61, 61.90, 52.38 and 47.61 with sperm in zero, 1st, 2nd and 3rd day storage respectively. Keywords: Preservation; Liquid semen; Quality; Artificial insemination; Sheep DOI: 10.3329/jbau.v7i2.4739 J. Bangladesh Agril. Univ. 7(2): 305-308, 2009

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