Assessment of cases of lichenoid granulomatous stomatitis in respect to demographics, histological features, and subcategories in known population

Introduction: Lichenoid granulomatous dermatitis (LGD) is widely encountered lesions with both oral as well as dermal manifestation. Present study was done to evaluate lichenoid granulomatous stomatitis cases. Materials and methods: 226 biopsies were exposed to special stains such as acid-fast bacilli (AFB), immunohistochemical staining for CD 68 and Grocott methenamine-silver (GMS), and periodic acid‐Schiff (PAS) stains. Results: Out of 226 patients, males were 84 and females were 142. Maximum cases were seen in age group 40–60 years (122) followed by >60 years (56) and 20–40 years (48). The common site was buccal mucosa seen in 128 (56.6%) cases followed by vestibule in 30 (13.2%), gingiva in 26 (11.5%), tongue in 20 (8.8%), lip in 12 (5.3%) and palate in 10 (4.4%). The common lesion was oral lichen planus seen in 142 (62.8%), carcinoma in situ in 12 (5.3%), squamous cell carcinoma in 8 (3.53%), pemphigus vulgaris in 10 (4.42%), leukoplakia in 24 (10.6%) and pemphigoid in 30 (13.2%) cases. Most lesions were of type I seen in 117 (51.7%) cases. Conclusion: Lichenoid granulomatous dermatitis poses variety of clinical as well as oral features. A long standing follows up and consideration of differential diagnosis is mandatory for better management of patients.

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Detection of Carriers in Glanzmann's Thrombasthenia

Thrombosis and Haemostasis ◽

10.1055/s-0038-1657357 ◽

1983 ◽

Vol 49 (03) ◽

pp. 182-186

Author(s):

G T E Zonneveld ◽

E F van Leeuwen ◽

A Sturk ◽

J W ten Cate

Keyword(s):

Bleeding Time ◽

Periodic Acid ◽

Type I ◽

Clot Retraction ◽

Periodic Acid Schiff ◽

Glanzmann’S Thrombasthenia ◽

Aggregation Response ◽

Glanzmann's Thrombasthenia ◽

Sds Page ◽

Reduced State

SummaryQuantitative glycoprotein (GP) analysis of whole platelets or platelet membranes was performed by SDS-polyacrylamide gelelectrophoresis (SDS-PAGE) and periodic acid Schiff staining in the families of two unrelated Glanzmann’s thrombasthenia (GT) patients. Each family consisted of two symptom free parents, a symptom free daughter and a GT daughter. All symptom free members had a normal bleeding time, clot retraction and platelet aggregation response to adenosine 5’-diphosphate (ADP), collagen and adrenalin. Platelet Zw* antigen was normally expressed in these subjects. GT patiens, classified as a type I and II subject, showed reduced amounts of GP lib and of GP nia. Analysis of isolated membranes in the non-reduced state, however, showed that the amount of GP Ilia was also reduced in three of the four parents, whereas one parent (of the GT type I patient) and the two unaffected daughters had normal amounts of GP Ilia. Quantitative SDS-PAGE may therefore provide a method for the detection of asymptomatic carriers in GT type I and II.

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Candida Chorioamnionitis in Mothers with Gestational Diabetes Mellitus: A Report of Two Cases

International Journal of Environmental Research and Public Health ◽

10.3390/ijerph18147450 ◽

2021 ◽

Vol 18 (14) ◽

pp. 7450

Author(s):

Elia Shazniza Shaaya ◽

Siti Atiqah Abdul Halim ◽

Ka Wen Leong ◽

Kevin Boon Ping Ku ◽

Pei Shan Lim ◽

...

Keyword(s):

Diabetes Mellitus ◽

Gestational Diabetes ◽

Gestational Diabetes Mellitus ◽

Umbilical Cord ◽

Neonatal Death ◽

Periodic Acid ◽

Careful Examination ◽

Periodic Acid Schiff ◽

History Of ◽

Methenamine Silver

Background:Candida chorioamnionitis is rarely encountered, even though vulvovaginal candidiasis incidence is about 15%. Interestingly, it has characteristic gross and histological findings on the umbilical cord that are not to be missed. Case Report: We report two cases of Candida chorioamnionitis with presence of multiple yellowish and red spots of the surface of the umbilical cord. Microscopically, these consist of microabscesses with evidence of fungal yeasts and pseudohyphae. The yeasts and pseudohyphae were highlighted by periodic acid– Schiff and Grocott methenamine silver histochemical stains. Both cases were associated with a history of gestational diabetes mellitus. Discussion: Peripheral funisitis is a characteristic feature of Candida chorioamnionitis. It is associated with high risk of adverse perinatal and neonatal outcomes, such as preterm delivery, stillbirth and neonatal death. We recommend careful examination of the umbilical cord of mothers with gestational diabetes mellitus.

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Alterações neoplásicas e não neoplásicas das mãos e dos pés de cães (2003-2016)

Pesquisa Veterinária Brasileira ◽

10.1590/1678-5150-pvb-5560 ◽

2018 ◽

Vol 38 (7) ◽

pp. 1394-1404

Author(s):

Lorena F. Silva ◽

Guilherme R. Blume ◽

Rômulo S.A. Eloi ◽

Jaqueline A. Lemos ◽

Anahí S. Silva ◽

...

Keyword(s):

Periodic Acid ◽

Labrador Retriever ◽

Periodic Acid Schiff ◽

Leishmania Spp ◽

Methenamine Silver

RESUMO: Mãos e pés de cães são comumente afetados por lesões neoplásicas e não neoplásicas. Estas alterações podem apresentar prognóstico histopatológico ou clínico ruim e a amputação tende a ser o tratamento de escolha. Estudos prévios avaliando a prevalência e os aspectos clínicopatológicos de alterações digitais em cães têm sido realizados em outros países, entretanto trabalhos similares não foram realizados no Brasil. O objetivo do presente estudo foi descrever e caracterizar histologicamente as lesões das mãos e pés de cães. Amostras de 105 lesões tumoriformes das mãos e pés de cães foram avaliadas. Essas amostras foram coletadas entre 2003 e 2016 e foram obtidas de três laboratórios brasileiros de diagnóstico veterinário. Todos espécimes foram fixados em formol tamponado 10%, processados rotineiramente e corados por hematoxilina-eosina. Adicionalmente, as colorações de azul de toluidina, Brown e Breen, periodic acid Schiff (PAS), Grocott Methenamine Silver (GMS) e Ziehl Neelsen foram utilizados em casos específicos. Imuno-histoquímica foi realizada usando os anticorpos anti-Leishmania spp., anti-CD117, anti-CD79, anti-CD3, anti-Melan A, anti-lisozima, anti-Pancytokeratin AE1/AE3 e anti-vimentina. Os neoplasmas (62,9%) foram as alterações mais frequentes, seguidas por inflamações (19%) e outras alterações (18,1%). Entre os neoplasmas, 40,9% foram mesenquimais, 39,4% epiteliais e 19,7% de células redondas. Todos os neoplasmas de células redondas e a maioria dos epiteliais e mesenquimais eram malignos. Não se observou predileção de prevalência dos neoplasmas em relação ao sexo. As raças Labrador Retriever, Schnauzer, Teckel, SRD, Pastor Alemão, Rottweiler e Pit Bull foram as mais afetadas. Fila Brasileiro, Pit Bull e Schnauzer tiveram alta incidência de neoplasmas mesenquimais, epiteliais e de células redondas, respectivamente. Inflamação foi mais comumente observada em cães Labrador Retriever e as outras alterações em Teckel, Labrador Retriever e SRD. A idade e o peso médio dos animais afetados foram de 8,4 anos e 28,5 kg, respectivamente. O diâmetro médio das lesões tumoriformes foi de 2,5 cm e as lesões neoplásicas apresentaram as maiores médias. As lesões ocorreram principalmente em animais de pelagem amarela. A maioria das biópsias incisionais (56,4%) e amputações (85,3%) consistiram de neoplasmas. O principal membro afetado foi o torácico direito e o dígito foi a estrutura anatômica acometida mais frequentemente. Carcinoma de células escamosas (14,2%) foi o neoplasma mais frequente, seguido do mastocitoma (7,6%), melanoma (7,6%) e sarcoma indiferenciado (7,6%). Em metade dos casos de inflamação, a lesão acometeu o folículo piloso e derme adjacente, e o infiltrado foi predominantemente piogranulomatoso ou lnfoplasmocítico. Cistos foliculares, calcinose circunscrita e acrocordoma foram as principais lesões não neoplásicas e não inflamatórias diagnosticadas.

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Primary cutaneous Aspergillus fumigatus infection in immunocompetent host

BMJ Case Reports ◽

10.1136/bcr-2019-233020 ◽

2020 ◽

Vol 13 (2) ◽

pp. e233020

Author(s):

Pradeep Kumar Mada ◽

Daniel Alexander Saldaña Koppel ◽

Majd Al Shaarani ◽

Andrew Stevenson Joel Chandranesan

Keyword(s):

Periodic Acid ◽

Fungal Spores ◽

Penetrating Trauma ◽

Trauma Patients ◽

Immunocompetent Host ◽

Skin Injury ◽

Periodic Acid Schiff ◽

Incision And Drainage ◽

Systemic Spread ◽

Methenamine Silver

Primary cutaneous aspergillosis (PCA) occurs through inoculation of fungal spores directly into the skin from the environment through disrupted skin such as in burns, surgery or penetrating trauma patients. Most cases reported in literature were in the immunocompromised, rarely in immunocompetent patients. The characteristic lesion of cutaneous aspergillosis is a black eschar on a red plaque, or nodule at the site of skin injury. The diagnosis of PCA can be made by identifying hyphal forms on routine H&E staining or special stains such as periodic acid-Schiff or Gomori methenamine-silver stains on skin biopsy and by fungal cultures. We report a case of an 80-year-old farmer who developed cutaneous aspergillosis after a surgical procedure without any systemic spread. The diagnosis was made by histopathology and tissue fungal cultures. He was treated with incision and drainage followed by oral voriconazole for 4 weeks; which led to clinical recovery.

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Special Staining of the Liquid-Based Cytopathology Test in Bronchoalveolar Lavage Fluid for Diagnosis of Invasive Pulmonary Aspergillosis with Nonneutropenic Patients

Canadian Respiratory Journal ◽

10.1155/2020/8243473 ◽

2020 ◽

Vol 2020 ◽

pp. 1-9

Author(s):

Yue Hu ◽

Lin Zheng ◽

Deng Pan ◽

Lei Shao ◽

Xianfa Xu ◽

...

Keyword(s):

Bronchoalveolar Lavage ◽

Sensitivity And Specificity ◽

Predictive Value ◽

Periodic Acid ◽

Invasive Pulmonary Aspergillosis ◽

Lavage Fluid ◽

Pulmonary Aspergillosis ◽

Periodic Acid Schiff ◽

Risk Patients ◽

Methenamine Silver

In recent years, various biomarkers have been gradually applied on bronchoalveolar lavage (BAL) fluid for the diagnosis of invasive pulmonary aspergillosis (IPA). The objective of this study is to assess the value of the liquid-based cytopathology test (LCT) for improving the identification of IPA in BAL fluid from possible IPA patients, following special staining with periodic acid-Schiff staining (PAS) or Grocott’s methenamine silver (GMS). A total of 47 consecutive possible IPA patients who underwent bronchoscopy with BAL fluid from January 2017 to December 2018 were included. 45 people had a pair of BAL fluid specimens and 2 patients had two BAL fluid specimens. The 49 pairs of BAL fluid specimens were processed for culture, tuberculosis acid fast staining smear, direct microbial smear, and LCT with special staining (PAS and GMS), respectively. Then, we compared the sensitivity and specificity of PAS and GMS in BAL fluid in high-risk patients. Among 47 possible IPA patients, 25 patients had proven/probable IPA, and 11 patients had other invasive fungal diseases. The sensitivity of GMS was higher than that of PAS (92.11% versus 81.58%; P=0.175). The specificity of GMS was 81.82%, which was higher than that of PAS (81.82% versus 72.73%; P=0.611). The negative predictive value (NPV) for PAS and GMS were 53.33% and 75.00%, respectively. The positive predictive value (PPV) for PAS and GMS were 91.18% and 94.59%, respectively. This study showed that special staining of LCT in BAL fluid may be a novel method for the diagnosis of IPA, and the GMS of LCT had higher sensitivity and specificity, which was superior to PAS.

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Rhinosinusitis in an Australian mare caused by Flavodon flavus, a recently recognized invasive fungal pathogen of the horse

Journal of Veterinary Diagnostic Investigation ◽

10.1177/1040638719897610 ◽

2019 ◽

Vol 32 (1) ◽

pp. 162-165

Author(s):

Willy W. Suen ◽

Steven Zedler ◽

Rochelle Price ◽

Tina Maguire ◽

Catriona Halliday ◽

...

Keyword(s):

Fungal Pathogen ◽

Periodic Acid ◽

The United States ◽

Nasal Passage ◽

Periodic Acid Schiff ◽

Ct Analysis ◽

Bone Lysis ◽

Methenamine Silver ◽

The Right ◽

Suppurative Inflammation

We describe herein the clinical, endoscopic, computed tomography (CT), pathologic, and microbiologic features of an infection caused by an under-recognized fungal pathogen, Flavodon flavus, in a 25-y-old Australian Quarter Horse. The horse had a unilateral obstructive nasal mass, resulting in stertor and dyspnea. On endoscopy, the mass was tan, multinodular, and completely obstructed the nasal passage. CT analysis revealed a large, soft tissue–attenuating and partially mineralized mass in the right nasal passage and dorsal-conchofrontal sinus, expanding into adjacent paranasal sinuses with associated bone lysis and rhinosinusitis. Histopathology of the mass on 2 occasions revealed suppurative inflammation initially, and pyogranulomatous inflammation subsequently. The inflammatory reaction surrounded numerous spherical fungal structures (~60–80 µm diameter) that stained positively on periodic acid–Schiff and Grocott methenamine silver stains. PCR for the fungal internal transcribed spacer 1 and 2 regions followed by Sanger sequencing on a cultured isolate identified the agent as F. flavus, which has only been reported previously as pathogenic in one horse in the United States, to our knowledge. Previous reports described this fungus as a nonpathogenic, environmental commensal fungus associated with insects and plants.

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Recruitment of single muscle fibers during submaximal cycling exercise

Journal of Applied Physiology ◽

10.1152/japplphysiol.00496.2007 ◽

2007 ◽

Vol 103 (5) ◽

pp. 1752-1756 ◽

Cited By ~ 33

Author(s):

T. M. Altenburg ◽

H. Degens ◽

W. van Mechelen ◽

A. J. Sargeant ◽

A. de Haan

Keyword(s):

Cross Sections ◽

Vastus Lateralis ◽

Periodic Acid ◽

Ratio Method ◽

Vastus Lateralis Muscle ◽

Type I ◽

Type Ii ◽

Periodic Acid Schiff ◽

Cycling Exercise ◽

Type Ii Fibers

In literature, an inconsistency exists in the submaximal exercise intensity at which type II fibers are activated. In the present study, the recruitment of type I and II fibers was investigated from the very beginning and throughout a 45-min cycle exercise at 75% of the maximal oxygen uptake, which corresponded to 38% of the maximal dynamic muscle force. Biopsies of the vastus lateralis muscle were taken from six subjects at rest and during the exercise, two at each time point. From the first biopsy single fibers were isolated and characterized as type I and II, and phosphocreatine-to-creatine (PCr/Cr) ratios and periodic acid-Schiff (PAS) stain intensities were measured. Cross sections were cut from the second biopsy, individual fibers were characterized as type I and II, and PAS stain intensities were measured. A decline in PCr/Cr ratio and in PAS stain intensity was used as indication of fiber recruitment. Within 1 min of exercise both type I and, although to a lesser extent, type II fibers were recruited. Furthermore, the PCr/Cr ratio revealed that the same proportion of fibers was recruited during the whole 45 min of exercise, indicating a rather constant recruitment. The PAS staining, however, proved inadequate to fully demonstrate fiber recruitment even after 45 min of exercise. We conclude that during cycling exercise a greater proportion of type II fibers is recruited than previously reported for isometric contractions, probably because of the dynamic character of the exercise. Furthermore, the PCr/Cr ratio method is more sensitive in determining fiber activation than the PAS stain intensity method.

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Identification of Brachyspira spp. in the cecum of broiler chickens using histology and in situ diagnostic assays

Semina Ciências Agrárias ◽

10.5433/1679-0359.2021v42n5p2813 ◽

2021 ◽

Vol 42 (5) ◽

pp. 2813-2824

Author(s):

Monica Regina de Matos ◽

◽

Aline Patrícia Grzegozevski ◽

Alessandra da Cruz ◽

Arthur Colombari Cheng ◽

...

Keyword(s):

Broiler Chickens ◽

Periodic Acid ◽

Goblet Cells ◽

Ffpe Tissue ◽

Economic Losses ◽

Periodic Acid Schiff ◽

Tissue Samples ◽

Rabbit Polyclonal Antibody ◽

Formalin Fixed Paraffin Embedded

The genus Brachyspira corresponds to the group of bacteria formerly classified into the genus Serpulina and includes several commensal and pathogenic intestinal spirochetes that affect pigs, poultry, and other animal species, including humans. In birds, some pathogenic species of this genus causes a condition known as avian intestinal spirochetosis, which remains underdiagnosed, thereby causing serious economic losses. Brachyspira is a fastidious organism that necessitates the employment of fast and efficient identification techniques. The aim of this study was to identify Brachyspira spp. using histology, immunohistochemistry (IHC) and fluorescence in situ hybridization (FISH) in formalin-fixed paraffin embedded (FFPE) tissue samples from the cecum of commercial poultry. Samples were collected from 129 birds aged between 35 and 45 days from commercial broiler farms. For evaluation, routine histology processing (H&E) and the histochemical technique, periodic acid–Schiff (PAS) were done. Additionally, FFPE tissue samples were evaluated for FISH and IHC. The histological lesions were analyzed and graded after H&E staining, and the goblet cells were counted and compared using PAS staining with the positive and negative samples obtained through FISH and IHC. For FISH, probes labeled with Brachyspira spp., B. pilosicoli, B. hyodysenteriae, and B. intermedia were used, whereas rabbit polyclonal antibody specific for Brachyspira spp. was used for IHC. Of 129 samples, 82 were positive with IHC and 86 were positive with FISH. The samples positive for the genus Brachyspira in the FISH technique were tested for B. pilosicoli, B. hyodysenteriae, and B. intermedia in which 56 were positive for B. pilosicoli, 75 for B. hyodysenteriae and 80 for B. intermedia. There was an increase in goblet cells in the samples positive for FISH and IHC. The techniques used were effective and gave corresponding results, thus serving as a fast and efficient tool for diagnosis.

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NANC nerve pathways controlling mucus glycoconjugate secretion into feline trachea

Journal of Applied Physiology ◽

10.1152/jappl.1992.73.2.625 ◽

1992 ◽

Vol 73 (2) ◽

pp. 625-630 ◽

Cited By ~ 8

Author(s):

D. C. Fung ◽

M. I. Allenby ◽

P. S. Richardson

Keyword(s):

Pulse Width ◽

Vagal Stimulation ◽

Periodic Acid ◽

Scintillation Counting ◽

Vagus Nerves ◽

Periodic Acid Schiff ◽

Chemical Assay ◽

Autonomic Blockade ◽

Tracheal Mucus

We used autonomic-blocking drugs to define nonadrenergic noncholinergic (NANC) vagus nerve pathways regulating tracheal mucus secretion. In anesthetized cats, mucus glycoconjugates, radiolabeled biosynthetically with [35S]sulfate and [3H]glucose, were washed from a tracheal segment in situ and dialyzed before scintillation counting and chemical assay with periodic acid-Schiff (PAS). Without autonomic blockade, vagal stimulation (9.5 Hz, 10 V, 2-ms pulse width, 10-min duration) increased outputs of radiolabeled and PAS-reactive glycoconjugates repeatably over four stimulation periods. In other animals, vagus nerves were stimulated with administration of autonomic blockers between stimulations. The first stimulation (no blockers) increased glycoconjugate output (delta 35S = 221 +/- 43.3%, delta 3H = 58 +/- 13.8%; delta PAS = +299 +/- 82.7%). Atropine, phentolamine, and propranolol reduced these responses (delta 35S = 67 +/- 15.6%; delta 3H = 26 +/- 5.3%; delta PAS = 88 +/- 25.6%). Guanethidine did not significantly lessen them further, although delta 3H was no longer significant. Ganglion blockade with hexamethonium prevented most of the remaining response to vagal stimulation (P less than 0.05 for diminution of delta 35S and delta PAS), but small effects persisted (delta 35S = 17 +/- 5.6%; delta PAS = 20 +/- 6.8%; P less than 0.05). We conclude that the main NANC vagal pathway controlling tracheal glycoconjugate secretion runs orthodromically.

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Crystal-Storing Histiocytosis as a Cause of Symptomatic Cardiac Mass

Archives of Pathology & Laboratory Medicine ◽

10.5858/133.11.1861 ◽

2009 ◽

Vol 133 (11) ◽

pp. 1861-1864 ◽

Cited By ~ 3

Author(s):

Charles J. Sailey ◽

Borislav A. Alexiev ◽

James S. Gammie ◽

Paula Pinell-Salles ◽

J. Lawrence Stafford ◽

...

Keyword(s):

Monoclonal Gammopathy ◽

Plasma Cells ◽

Periodic Acid ◽

Right Atrial ◽

Prior History ◽

Low Grade ◽

Periodic Acid Schiff ◽

Right Atrial Mass ◽

History Of

Abstract Crystal-storing histiocytosis is a rare disorder that is typically associated with low-grade B-cell lymphomas and monoclonal gammopathy. We present a 64-year-old man with a prior history of weakness and weight loss and hematologic evaluation that had revealed immunoglobulin G κ monoclonal light chains in the serum and negative bone marrow biopsy. He presented with supraventricular tachyarrhythmia and a right atrial mass seen on echocardiogram and excised surgically. Histologically, the tumor was composed of sheets of macrophages infiltrating the atrial myocardium. The histiocytes were filled with multiple needle-shaped, periodic acid-Schiff–negative crystals. These cells and associated plasma cells failed to show clonal light chain restriction by in situ hybridization or immunohistochemistry, and there was no area of lymphoma in the tumor. Ultrastructural examination showed numerous sticklike, trapezoidal, or polygonal dense crystals in the cytoplasm of histiocytes corroborating the diagnosis of crystal-storing histiocytosis. Although rare, crystal-storing histiocytosis should be included in the differential diagnosis of heart masses in patients with hematologic conditions associated with monoclonal gammopathy.

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