Development of HPTLC method for quantification of vasicine in marketed Herbal formulations containing Adhatoda vasica

Objective: In the present study a novel stability-indicating high-performance thin-layer chromatography (HPTLC) method for quantitative determination of Swertiamarin (SW) in bulk drug and formulation has been developed and validated as per ICH guideline Q2 (R1) for global acceptance of standardized herbal formulations.Methods: HPTLC method is developed and validated using solvent ethyl acetate: ethanol: chloroform (3:2.5:4.5 v/v/v) (Rf of SW 0.65±0.04) in the absorbance mode at 243 nm. Various forced degradation conditions were used to check degradation of drug.Results: The method showed a good linear relationship (r2 = 0.9990) in the concentration range 200-700 ng per spot. It was found to be linear, accurate, precise and specific.Conclusion: It can be applied for quality control as well as for stability testing of different dosage forms containing swertiamarin. The developed method is validated as per ICH guideline Q2(R1) for global acceptance of standardized herbal formulations.

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Quantification of Physiologically Available Glycyrrhizin in Anti-Stress Herbal Formulations by Validated HPTLC Method

Asian Journal of Chemistry ◽

10.14233/ajchem.2014.16111 ◽

2014 ◽

Vol 26 (3) ◽

pp. 874-878 ◽

Cited By ~ 1

Author(s):

Nasir A. Siddiqui ◽

Perwez Alam ◽

Azmat Ali Khan ◽

Ateeque Ahmad ◽

Adnan J. Alrehaily ◽

...

Keyword(s):

Herbal Formulations ◽

Hptlc Method

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A HPTLC Method for Determination of Anethole in Essential Oil, Methanolic Extract of Foeniculum vulgare and Commercial Herbal Products

Journal of Pharmaceutical Research International ◽

10.9734/jpri/2020/v32i1230561 ◽

2020 ◽

pp. 68-75 ◽

Cited By ~ 1

Author(s):

Prawez Alam ◽

Mohammad H. Alqarni ◽

Pravej Alam ◽

Ahmed I. Foudah ◽

Mohammed Ghazwani

Keyword(s):

Essential Oil ◽

Aromatic Plants ◽

Foeniculum Vulgare ◽

Herbal Products ◽

Ich Guidelines ◽

Oil Extract ◽

Satisfactory Relationship ◽

Herbal Formulations ◽

Hptlc Method

A rapid and feasible method of HPTLC is standardized for quantification of anethole in essential oil’s extract and from herbal formulations of fennel seed. The developed densitometric HPTLC method was performed to estimate the existence of anethole in the essential oil, extract and herbal formulations of fennel with the optimized concentration of hexane: Ethyl acetate (8:2%, v/v, mobile phase) on glass coated silica gel 60 F254 plates (20 × 10 cm) scanned with the absorbance of λ260 nm under densitometric condition. The Linearity of regressions revealed a satisfactory relationship between peak area and concentration of anethole in between the range of 100-600 ng/spot. This reliable method was validated as per the ICH guidelines to fulfill the necessary parameters such as accuracy and robustness. The amount of anethole in essential oil (0.098 ± 0.002%), extract (0.101 ± 0.004%) and three herbal formulations A (0.024 ± 0.004%), C (0.019 ± 0.002%) while anethole is not detected in B formulations from fennel seed was completely estimated by the developed method. The standardized methods and its validation gave new insights of HPTLC based detection and quantification of anethole in other aromatic plants as well as in other pharmacological formulations.

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A systematic ethnobotanical review of Adhatoda vasica (L.), Nees.

Cellular and Molecular Biology ◽

10.14715/cmb/2021.67.4.28 ◽

2022 ◽

Vol 67 (4) ◽

pp. 248-263

Author(s):

Ambreen Shoaib

Keyword(s):

Bacterial Infections ◽

Indian Subcontinent ◽

Human Beings ◽

Native Plant ◽

Active Constituent ◽

Adhatoda Vasica ◽

Toxicological Studies ◽

The Family ◽

Overall Evaluation ◽

Herbal Formulations

Adhatoda vasica (L.), Nees belonging to the family Acanthaceae is a shrub with opposite ascending branches. The plant has been used in the indigenous system of medicine in India for near about 2500 years. It is a well-known plant as a drug in Ayurvedic and Unani medicines. Traditionally it was used for the treatment of various acute and chronic diseases and showed strong pharmacological activity particularly for bronchial infections, cough, bacterial infections, reproductive disorders, cardiac diseases and many more. Various phytochemicals like alkaloids, flavonoids, tannins, etc. were obtained from Adhatoda vasica (A. vasica). The active constituent of the plant is vasicine, l-vasicinone, deoxyvasicine, maiontone, vasicinolone and vasicinol etc. This review consists of updated information on the phyto-constituents isolated from A. vasica and their potential role in the treatment of various ailments traditionally and medically. Based on the critical review it was concluded that there is not sufficient scientifically strong evidence to explain that A. vasica extract, could be harmful to human beings especially in pregnant women. Major data on traditional uses as well as toxicological studies, evaluated various correctness, relevance, importance, and reliability for the overall evaluation of A. vasica safety. Numerous clinical trials are conducted around the globe on the herbal formulations of vasaka. This review includes strong data about phytochemical and ethnopharmacological studies that indicate that A. vasica is a versatile native plant of the Indian subcontinent having a commercial reputation and thus can be encouraged for diversified applications like medicinal and other potential uses.

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Simultaneous HPTLC Densitometric Estimation of KBA and AKBA from Boswellia serrata

Current Analytical Chemistry ◽

10.2174/1573411014666180704123521 ◽

2018 ◽

Vol 15 (1) ◽

pp. 84-91 ◽

Cited By ~ 2

Author(s):

Meenu Mehta ◽

Munish Garg ◽

Kamal Dua ◽

Saurabh Satija

Keyword(s):

Bioactive Compounds ◽

High Performance ◽

Limit Of Detection ◽

Control Analysis ◽

Limit Of Quantification ◽

Boswellia Serrata ◽

Quality Control Analysis ◽

Layer Chromatography ◽

Herbal Formulations ◽

Hptlc Method

Background: Boswellic acids (BAs) are extracted from oleo gum of Boswellia serrata and are utilized as potential anti-inflammatory, hypolipidemic, immunomodulatory and antitumor specialists. The present examination was meant to assess KBA and AKBA in Boswellia serrata separate by High-Performance Thin Layer Chromatography (HPTLC). Methods: The separation of bioactive compounds was performed utilizing mobile phase glacial acetic acid, n-hexane, ethyl acetate and toluene (0.3: 1: 8: 2) (v/v/v/v) and distinguished at wavelength 254 nm. The technique was approved for linearity, precision, accuracy, limit of detection (LOD), limit of quantification (LOQ), and so forth by International Conference on Harmonization guidelines. Results: The calibration range was observed to be 2- 14 μg/band for both the bioactive compounds. KBA was isolated with an Rf estimation of 0.39 ± 0.02 and AKBA with an Rf estimation of 0.42 ± 0.02. The accuracy was seen to be as high as 99.17% and 97.42 for KBA and KBA, respectively. The percentage RSD value for intra-day and between day varieties was under 2%. The system indicated high affectability and specificity. Conclusion: The developed HPTLC method was simple, precise, robust, specific, rapid, and costeffective and could be used for quality control analysis and quantification of KBA and AKBA in different herbal formulations containing the plant species.

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Quality of cranberry-derived products: one HPTLC method for identification and detection of adulterants

10.1055/s-0039-3399769 ◽

2019 ◽

Author(s):

DA Frommenwiler ◽

M Monagas ◽

E Reich

Keyword(s):

Hptlc Method

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Development of Validated Stability Indicating HPTLC Method for Assay of Ozagrel and its Pharmaceutical Formulations

International Journal of Scientific Research in Science Engineering and Technology ◽

10.32628/ijsrset11841111 ◽

2018 ◽

pp. 36-48 ◽

Cited By ~ 2

Author(s):

Vishal N Kushare ◽

Sachin S Kushare

Keyword(s):

High Performance ◽

Linear Regression Analysis ◽

Pharmaceutical Formulations ◽

Base Hydrolysis ◽

Assay Method ◽

Related Substance ◽

Stability Indicating ◽

Regular Production ◽

Hptlc Method

The present paper describes stability indicating high-performance thin-layer chromatography (HPTLC) assay method for Ozagrel in bulk drugs. The method employed TLC aluminium plates precoated with silica gel 60F-254 as the stationary phase. The solvent system consisted of toluene: methanol: triethylamine (6.5: 4.0: 0.1 v/v/v). The system was found to give compact spot for Ozagrel (Rf value of 0.40 ± 0.010). Densitometric analysis of Ozagrel was carried out in the absorbance mode at 280 nm. The linear regression analysis data for the calibration plots showed good linear relationship with r2 = 0.999 with respect to peak area in the concentration range 30 - 120 ng/spot. The developed HPTLC method was validated with respect to accuracy, precision, recovery and robustness. Also to determine related substance and assay determination of Ozagrel that can be used to evaluate the quality of regular production samples. The developed method can also be conveniently used for the assay determination of Ozagrel in pharmaceutical formulations. The limits of detection and quantitation were 4.069 and 12.332 ng/spot, respectively by height. Ozagrel was subjected to acid and alkali hydrolysis, oxidation, photochemical and thermal degradation. The drug undergoes degradation under acidic, basic, oxidation and heat conditions. This indicates that the drug is susceptible to acid, base hydrolysis, oxidation and heat. Statistical analysis proves that the method is repeatable, selective and accurate for the estimation of said drug. The proposed developed HPTLC method can be applied for identification and quantitative determination of Ozagrel in bulk drug and tablet formulation.

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