Quality of cranberry-derived products: one HPTLC method for identification and detection of adulterants

2019 ◽  
Author(s):  
DA Frommenwiler ◽  
M Monagas ◽  
E Reich
Keyword(s):  
Hptlc Method

scholarly journals Chromatographic Profiling of Rose Petals in Unani Formulations (Gulkand, Arq-e-Gulab, and Rose Sharbat) Using HPTLC and GC–MS

2020 ◽  
Vol 103 (3) ◽  
pp. 684-691
Author(s):  
Rabea Parveen ◽  
Sultan Zahiruddin ◽  
Akshay Charegaonkar ◽  
Abhijeet Khale ◽  
Saikat Mallick
Keyword(s):  
Traditional Medicine ◽  
Chromatographic Analysis ◽  
Herbal Products ◽  
Unani Medicine ◽  
Ms Analysis ◽  
Rose Petals ◽  
The Rose ◽  
Hptlc Method ◽  
Chromatographic Profiling

Abstract Background: One of the most precious systems of traditional medicine is the Unani system of medicine. A wide variety of formulations indigenous to the Unani medicine have been preserved over the years. These formulations are potent and effective even after ages of postformulation. Rose petals are an example of such an herb, which is extensively and popularly used in Unani formulations for edible and cosmetic purposes. Rose petals are rich in terpenes, glycosides, flavonoids, and anthocyanins. Objective: The aim of this study was to characterize the phytochemical profiling of different rose varieties and their marketed formulations. Method: HPTLC method was developed for detecting overall profile and assessing variations among the rose varieties available in market along with the popular formulations of rose such as gulkand (Brand A and Brand B), Arq-e-gulab or Gulab Jal (Brand C), and sharbat (Brand D). GC–MS analysis was also carried out for fingerprinting of rose varieties and formulations. Results: HPTLC and GC–MS fingerprinting showed some common peaks in rose samples as well as in the formulation samples. The methods also gave different peaks for the adulterant that might be used in place of rose. Conclusions: Both the methods could be used for standardization of herbal products containing rose as one of the ingredients and also used to check for the adulteration. Highlights: The current advanced chromatographic analysis is a valuable tool to determine the quality of the formulation.

Comprehensive HPTLC Fingerprinting for Quality Control of an Herbal Drug – The Case of Angelica gigas Root

Planta Medica ◽  
2018 ◽  
Vol 84 (06/07) ◽  
pp. 465-474 ◽  
Author(s):  
Débora Frommenwiler ◽  
Jonghwan Kim ◽  
Chang-Soo Yook ◽  
Thi Tran ◽  
Salvador Cañigueral ◽  
...  
Keyword(s):  
Quality Control ◽  
Reference Material ◽  
Herbal Drugs ◽  
Proof Of Concept ◽  
Herbal Drug ◽  
Angelica Gigas ◽  
Decursinol Angelate ◽  
Suitable Reference ◽  
Hptlc Method

AbstractThe quality of herbal drugs is usually controlled using several tests recommended in a monograph. HPTLC is the method of choice for identification in many pharmacopoeias. If combined with a suitable reference material for comparison, HPTLC can provide information beyond identification and thus may simplify quality control. This paper describes, as a proof of concept, how HPTLC can be applied to define specifications for an herbal reference material and to control the quality of an herbal drug according to these specifications. Based on multiple batches of cultivated Angelica gigas root, a specific HPTLC method for identification was optimized. This method can distinguish 27 related species. It also can detect the presence of mixtures of A. gigas with two other Angelica species traded as “Dang gui” and is suitable as well for quantitative assessment of samples in a test for minimum content of the sum of decursin and decursinol angelate. The new concept of “comprehensive HPTLC fingerprinting” is proposed: HPTLC fingerprints (images), which are used for identification, are converted into peak profiles and the intensities of selected zones are quantitatively compared to those of the corresponding zones of the reference material. Following a collaborative trial involving three laboratories in three countries, the method was applied to check the quality of further candidates for establishing an appropriate reference material. In conclusion, this case demonstrates that a single HPTLC analysis can provide information about identity, purity, and minimum content of markers of an herbal drug.

scholarly journals PHARMACEUTICO-ANALYTICAL STUDY AND QUALITY STANDARD DETEC-TION OF JATYADI GHRITA BY HPTLC METHOD

2020 ◽  
Vol p4 (06) ◽  
pp. 2462-2468
Author(s):  
Priyanka Sharma ◽  
K. Shankar Rao
Keyword(s):  
Acid Value ◽  
Quality Standard ◽  
External Application ◽  
Therapeutic Value ◽  
Instrument Analysis ◽  
Laboratory Reference ◽  
Finger Printing ◽  
Total Fatty Matter ◽  
Hptlc Method

In the era of commercialization quality control and standardization of herbal formulation is essential in or-der to assess the quality of drugs for therapeutic value. Jatyadi Ghrita is a ghee based Ayurvedic formula-tion useful for treatment of all kind of wounds by external application found in classical texts of Ayurveda. In this study an attempt has been made to develop standard for Jatyadi Ghrita by HPTLC method. It was prepared by standard laboratory reference of Ayurvedic formulary of India in three batches in RS & BK Laboratory NIA, Jaipur. The physicochemical tests performed on different samples of Jatyadi Ghrita such as acid value, saponification value, Total fatty matter, melting point, viscosity etc. Further standardization and comparative evaluation of laboratory sample and marketed sample done by instrument analysis per-formed on HPTLC finger printing profile. Data has been provided to demonstrate applicability of the methods to standardization of Jatyadi Ghrita.

scholarly journals Quantification of Glycyrrhizin in Glycyrrhiza Glabra Extract by Validated HPTLC Densitometry

2010 ◽  
Vol 93 (2) ◽  
pp. 492-495 ◽  
Author(s):  
Arunava Gantait ◽  
Subrata Pandit ◽  
Neelesh K Nema ◽  
Pulok K Mukjerjee
Keyword(s):  
Area Under Curve ◽  
Cost Effective ◽  
Solvent System ◽  
Quality Measure ◽  
Glycyrrhiza Glabra ◽  
Hydroalcoholic Extract ◽  
Linearity Range ◽  
Hptlc Method ◽  
Bioactive Constituent

Abstract Glycyrrhiza Glabra Linn (Family-Fabaceae) is active as an anti-allergic, anti-inflammatory, spasmolytic, mild laxative, antistress, antidepressive, antiulcer, liver protective, estrogenic, emmenagogue, and antidiabetic substance, and is widely used in the Indian system of medicine. The major bioactive constituent is glycyrrhizin. A simple HPTLC method has been developed to control the quality of raw as well as finished glycyrrhiza using glycyrrhizin as the bioactive marker. The solvent system was optimized to chloroformmethanolwater (65 + 36 + 7.5, v/v/v). Extract and standard were dissolved in 70 methanol and applied on a precoated TLC plate. After development, the plate was scanned at 254 nm to create a chromatogram, then the quantity of glycyrrhizin was determined in the extract. The method was validated in terms of specificity, linearity, precision, LOD, and LOQ. Linearity range was found to be 0.964.80 g per spot. The linearity relationship was described by the equation: Y 612.706 + 1.091X (with r 0.99904 and SD 2.52), where Y is the area under curve and X is the amount of glycyrrhizin (ng). The amount of glycyrrhizin found in the extract was 9.1. Thus, the method provides a rapid and cost-effective quality measure for Glycyrrhiza Glabra hydroalcoholic extract.

Immunoferritin localization of intracellular antigens in positively stained frozen sections

1978 ◽  
Vol 36 (2) ◽  
pp. 168-169
Author(s):  
K. T. Tokuyasu
Keyword(s):  
Surface Tension ◽  
Water Surface ◽  
Thin Layers ◽  
The Other ◽  
Positive Staining ◽  
Frozen Sections ◽  
The Past ◽  
Ultrathin Frozen Sections ◽  
Definition Of

During the past investigations of immunoferritin localization of intracellular antigens in ultrathin frozen sections, we found that the degree of negative staining required to delineate u1trastructural details was often too dense for the recognition of ferritin particles. The quality of positive staining of ultrathin frozen sections, on the other hand, has generally been far inferior to that attainable in conventional plastic embedded sections, particularly in the definition of membranes. As we discussed before, a main cause of this difficulty seemed to be the vulnerability of frozen sections to the damaging effects of air-water surface tension at the time of drying of the sections.Indeed, we found that the quality of positive staining is greatly improved when positively stained frozen sections are protected against the effects of surface tension by embedding them in thin layers of mechanically stable materials at the time of drying (unpublished).

Lithography below 100 nm

1983 ◽  
Vol 41 ◽  
pp. 96-99
Author(s):  
L. D. Jackel
Keyword(s):  
Spatial Distribution ◽  
Electron Beam ◽  
Electron Scattering ◽  
Electron Beam Lithography ◽  
Substrate Material ◽  
Local Electron ◽  
Electron Dose ◽  
Positive Resist ◽  
Exposure Process

Most production electron beam lithography systems can pattern minimum features a few tenths of a micron across. Linewidth in these systems is usually limited by the quality of the exposing beam and by electron scattering in the resist and substrate. By using a smaller spot along with exposure techniques that minimize scattering and its effects, laboratory e-beam lithography systems can now make features hundredths of a micron wide on standard substrate material. This talk will outline sane of these high- resolution e-beam lithography techniques.We first consider parameters of the exposure process that limit resolution in organic resists. For concreteness suppose that we have a “positive” resist in which exposing electrons break bonds in the resist molecules thus increasing the exposed resist's solubility in a developer. Ihe attainable resolution is obviously limited by the overall width of the exposing beam, but the spatial distribution of the beam intensity, the beam “profile” , also contributes to the resolution. Depending on the local electron dose, more or less resist bonds are broken resulting in slower or faster dissolution in the developer.

Convergent Beam Electron Diffraction

1978 ◽  
Vol 36 (3) ◽  
pp. 304-315
Author(s):  
G. Lehmpfuhl
Keyword(s):  
Electron Diffraction ◽  
Diffraction Pattern ◽  
Crystal Surface ◽  
Diffraction Spot ◽  
Crystal Thickness ◽  
Large Area ◽  
Electron Microscopic ◽  
Additional Information ◽  
Microscopic Investigations

Introduction In electron microscopic investigations of crystalline specimens the direct observation of the electron diffraction pattern gives additional information about the specimen. The quality of this information depends on the quality of the crystals or the crystal area contributing to the diffraction pattern. By selected area diffraction in a conventional electron microscope, specimen areas as small as 1 µ in diameter can be investigated. It is well known that crystal areas of that size which must be thin enough (in the order of 1000 Å) for electron microscopic investigations are normally somewhat distorted by bending, or they are not homogeneous. Furthermore, the crystal surface is not well defined over such a large area. These are facts which cause reduction of information in the diffraction pattern. The intensity of a diffraction spot, for example, depends on the crystal thickness. If the thickness is not uniform over the investigated area, one observes an averaged intensity, so that the intensity distribution in the diffraction pattern cannot be used for an analysis unless additional information is available.

Observability of Very Thick Specimen by Using Transmission Scanning Electron Microscope

1971 ◽  
Vol 29 ◽  
pp. 26-27
Author(s):  
K. Shibatomi ◽  
T. Yamanoto ◽  
H. Koike
Keyword(s):  
Electron Microscope ◽  
Image Quality ◽  
Scanning Electron Microscope ◽  
Chromatic Aberration ◽  
Image Contrast ◽  
Objective Lens ◽  
Thick Specimen ◽  
Transmission Electron ◽  
Scanning Electron

In the observation of a thick specimen by means of a transmission electron microscope, the intensity of electrons passing through the objective lens aperture is greatly reduced. So that the image is almost invisible. In addition to this fact, it have been reported that a chromatic aberration causes the deterioration of the image contrast rather than that of the resolution. The scanning electron microscope is, however, capable of electrically amplifying the signal of the decreasing intensity, and also free from a chromatic aberration so that the deterioration of the image contrast due to the aberration can be prevented. The electrical improvement of the image quality can be carried out by using the fascionating features of the SEM, that is, the amplification of a weak in-put signal forming the image and the descriminating action of the heigh level signal of the background. This paper reports some of the experimental results about the thickness dependence of the observability and quality of the image in the case of the transmission SEM.

The Current Situation in Chemical Stabilization of Biological Materials

1972 ◽  
Vol 30 ◽  
pp. 132-133
Author(s):  
John H. Luft
Keyword(s):  
Electron Microscopy ◽  
Electron Microscope ◽  
Osmium Tetroxide ◽  
Molecular Level ◽  
Cross Linking ◽  
Chemical Stabilization ◽  
Specimen Preparation ◽  
Sufficient Condition ◽  
Radio Telescopes

With information processing devices such as radio telescopes, microscopes or hi-fi systems, the quality of the output often is limited by distortion or noise introduced at the input stage of the device. This analogy can be extended usefully to specimen preparation for the electron microscope; fixation, which initiates the processing sequence, is the single most important step and, unfortunately, is the least well understood. Although there is an abundance of fixation mixtures recommended in the light microscopy literature, osmium tetroxide and glutaraldehyde are favored for electron microscopy. These fixatives react vigorously with proteins at the molecular level. There is clear evidence for the cross-linking of proteins both by osmium tetroxide and glutaraldehyde and cross-linking may be a necessary if not sufficient condition to define fixatives as a class.

Sign in / Sign up

Export Citation Format

Share Document