Antioxidant activity of some Scorzonera species and quantitative analysis of chlorogenic acid

AbstractMulberry leaves are used in traditional Chinese medicine and contain numerous active substances that are known to be beneficial for human health. The aim of this study was to investigate the phenolic compositions and antioxidant activities of the leaves from 23 mulberry cultivars. Qualitative LC-ESI-QTOF analysis revealed the presence of 11 phenolic compounds in the free phenolic extracts and 10 phenolic compounds in the bound fractions. Chlorogenic acid and caffeic acid were the major components in the free and bound fractions, respectively. The results revealed that the changguosang cultivar from Taiwan contained the greatest content of phenolic compounds as well as the highest antioxidant activity among the 23 cultivars examined, as determined using three separate antioxidant assays. The isoquercitrin, chlorogenic acid, and rutin contents of the free phenolic extracts displayed significant correlations with the antioxidant activities, while syringic acid and rutin were the main contributors to the antioxidant activities of the bound phenolic fractions. The obtained results demonstrate that mulberry leaves contain a variety of beneficial phenolic substances and may be suitable for further development as a herbal medicine.

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COMPARATIVE ANALYSIS OF MORPHOLOGY AND PHYTOCHEMICAL CONSTITUENTS IN DIFFERENT POPULATIONS AND MORPHOTYPES OF DATURA INNOXIA MILL. AND DATURA METEL L. FROM PUNJAB PLAINS

Asian Journal of Pharmaceutical and Clinical Research ◽

10.22159/ajpcr.2019.v12i1.27720 ◽

2019 ◽

Vol 12 (1) ◽

pp. 193 ◽

Cited By ~ 2

Author(s):

Mahinder Partap ◽

Raghbir Chand Gupta ◽

Saroj Kumar Pradhan

Keyword(s):

Antioxidant Activity ◽

Comparative Analysis ◽

Chlorogenic Acid ◽

Caffeic Acid ◽

Health Benefit ◽

Bioactive Metabolites ◽

Datura Innoxia ◽

Datura Metel ◽

Different Populations ◽

Phytochemical Constituents

Objective: Comparative analysis of morphology and phytochemical constituents in different populations and morphotypes of Datura innoxia Mill. and Datura metel L. from Punjab plains.Methods: Morphological analyses of different populations have been done. Methanol extracts of seeds and roots of different populations have been prepared and total phenols, flavonoid contents were measured through spectrophotometry. Antioxidant activity was studied by 2, 2-diphenyl-1- picrylhydrazyl radical scavenging activity and total antioxidant capacity. Two major compounds, caffeic acid and chlorogenic acid were quantified by high-performance thin-layer chromatography (HPTLC) analyses.Results: Two morphotypes of D. metel were reported in the present study. Out of five different populations of D. metel and D. innoxia, the wild populations have more bioactive compound as compared to the cultivated ones from the detailed phytochemical investigation. Pharmacologically important two marker compounds chlorogenic acid and caffeic acid has been identified and quantified by HPTLC technique.Conclusion: Variation in terms of morphology and secondary metabolites exists among the different populations of Datura spp. Among the two plant parts studied, seeds have the maximum amount of bioactive metabolites and antioxidant activity. This study revealed that chlorogenic acid and caffeic acid are the potential polyphenolic compounds in Datura spp. It has been found that the antioxidant activity of plant is due to its polyphenol contents, which provides insight to various researchers to work on it as it imparts health benefit.

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Optimization of the Extraction Technology and Assessment of Antioxidant Activity of Chlorogenic Acid-Rich Extracts From Eucommia ulmoides Leaves

Natural Product Communications ◽

10.1177/1934578x211046105 ◽

2021 ◽

Vol 16 (10) ◽

pp. 1934578X2110461

Author(s):

Hua Jiang ◽

Jun Li ◽

Ning Zhang ◽

Hai-Yang He ◽

Jia-Min An ◽

...

Keyword(s):

Antioxidant Activity ◽

Chlorogenic Acid ◽

Ph Value ◽

Extraction Process ◽

Eucommia Ulmoides ◽

Extraction Technology ◽

Dpph Method ◽

Contour Plots ◽

Technology And Assessment ◽

Design Optimum

Chlorogenic acid has been proved to have cardiovascular protection, antibacterial, antiviral, hemostatic, and hypolipidemia effects. Modern scientific research on the bioactivity of chlorogenic acid has been extended to the fields of food, medicine, health care and daily-use chemical industry. The aim of this research was to optimize the extraction conditions for chlorogenic acid from Eucommia ulmoides (Eucommiaceae) leaves. The significant variables were screened and optimized by a combination of Plackett-Burman test and Box-Behnken design. Optimum extraction parameters with ethanol concentration of 50%, solvent pH value of 3, and particle size of 60 mesh were determined according to variance analysis and contour plots. Under these conditions, the yield of chlorogenic acid was up to 4.36 mg/g, which was basically consistent with the theoretical prediction value of 4.50 mg/g. This study also proved the potential antioxidant activity of E. ulmoides leaves. The optimal extract of E. ulmoides leaves rich in chlorogenic acid showed the highest antioxidant activity in the FRAP method, which was 219.8 μM Trolox equivalents (TE) per g extract weight (EW) (μM TE/g EW). The DPPH method gave a similar value (168 μM TE/g EW) to the ABTS method (152 μM TE/g EW). The established extraction process was efficient in the recovery of chlorogenic acid from E. ulmoides leaves, encouraging its valorization as a cheap and sustainable alternative for the isolation of chlorogenic acid.

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Zn(II) Complex of Plant Phenolic Chlorogenic Acid: Antioxidant, Antimicrobial and Structural Studies

Materials ◽

10.3390/ma13173745 ◽

2020 ◽

Vol 13 (17) ◽

pp. 3745

Author(s):

Monika Kalinowska ◽

Justyna Sienkiewicz-Gromiuk ◽

Grzegorz Świderski ◽

Anna Pietryczuk ◽

Adam Cudowski ◽

...

Keyword(s):

Antioxidant Activity ◽

Chlorogenic Acid ◽

Density Functional ◽

Salmonella Enteritidis ◽

Antioxidant Properties ◽

Antimicrobial Activities ◽

Butylated Hydroxytoluene ◽

Antioxidant Power ◽

Ferric Reducing Antioxidant Power ◽

Thermochemical Parameters

The structure of the Zn(II) complex of 5-caffeoylquinic acid (chlorogenic acid, 5-CQA) and the type of interaction between the Zn(II) cation and the ligand were studied by means of various experimental and theoretical methods, i.e., electronic absorption spectroscopy UV/Vis, infrared spectroscopy FT-IR, elemental, thermogravimetric and density functional theory (DFT) calculations at B3LYP/6-31G(d) level. DPPH (2,2-diphenyl-1-picrylhydrazyl), ABTS (2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid), FRAP (ferric reducing antioxidant power), CUPRAC (cupric reducing antioxidant power) and trolox oxidation assays were applied in study of the anti-/pro-oxidant properties of Zn(II) 5-CQA and 5-CQA. The antimicrobial activity of these compounds against Escherichia coli, Pseudomonas aeruginosa, Bacillus subtilis, Staphylococcus aureus, Salmonella enteritidis and Candida albicans was tested. An effect of Zn(II) chelation by chlorogenic acid on the anti-/pro-oxidant and antimicrobial activities of the ligand was discussed. Moreover, the mechanism of the antioxidant properties of Zn(II) 5-CQA and 5-CQA were studied on the basis of the theoretical energy descriptors and thermochemical parameters. Zn(II) chlorogenate showed better antioxidant activity than chlorogenic acid and commonly applied natural (L-ascorbic acid) and synthetic antioxidants (butylated hydroxyanisol (BHA) and butylated hydroxytoluene (BHT)). The pro-oxidant activity of Zn(II) 5-CQA was higher than the ligand and increased with the rise of the compound concentration The type of Zn(II) coordination by the chlorogenate ligand strongly affected the antioxidant activity of the complex.

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