Comparative quantification of phosphatidylcholine in sea urchins eggs by instrumental TLC with various detection techniques

Planta Medica ◽  
2010 ◽  
Vol 76 (12) ◽  
Author(s):  
S Ivanova ◽  
I Urakova ◽  
O Pozharitskaya ◽  
A Shikov ◽  
V Makarov
Author(s):  
D. F. Blake ◽  
L. F. Allard ◽  
D. R. Peacor

Echinodermata is a phylum of marine invertebrates which has been extant since Cambrian time (c.a. 500 m.y. before the present). Modern examples of echinoderms include sea urchins, sea stars, and sea lilies (crinoids). The endoskeletons of echinoderms are composed of plates or ossicles (Fig. 1) which are with few exceptions, porous, single crystals of high-magnesian calcite. Despite their single crystal nature, fracture surfaces do not exhibit the near-perfect {10.4} cleavage characteristic of inorganic calcite. This paradoxical mix of biogenic and inorganic features has prompted much recent work on echinoderm skeletal crystallography. Furthermore, fossil echinoderm hard parts comprise a volumetrically significant portion of some marine limestones sequences. The ultrastructural and microchemical characterization of modern skeletal material should lend insight into: 1). The nature of the biogenic processes involved, for example, the relationship of Mg heterogeneity to morphological and structural features in modern echinoderm material, and 2). The nature of the diagenetic changes undergone by their ancient, fossilized counterparts. In this study, high resolution TEM (HRTEM), high voltage TEM (HVTEM), and STEM microanalysis are used to characterize tha ultrastructural and microchemical composition of skeletal elements of the modern crinoid Neocrinus blakei.


Author(s):  
Frank J. Longo

Measurement of the egg's electrical activity, the fertilization potential or the activation current (in voltage clamped eggs), provides a means of detecting the earliest perceivable response of the egg to the fertilizing sperm. By using the electrical physiological record as a “real time” indicator of the instant of electrical continuity between the gametes, eggs can be inseminated with sperm at lower, more physiological densities, thereby assuring that only one sperm interacts with the egg. Integrating techniques of intracellular electrophysiological recording, video-imaging, and electron microscopy, we are able to identify the fertilizing sperm precisely and correlate the status of gamete organelles with the first indication (fertilization potential/activation current) of the egg's response to the attached sperm. Hence, this integrated system provides improved temporal and spatial resolution of morphological changes at the site of gamete interaction, under a variety of experimental conditions. Using these integrated techniques, we have investigated when sperm-egg plasma membrane fusion occurs in sea urchins with respect to the onset of the egg's change in electrical activity.


1983 ◽  
Vol 44 (C7) ◽  
pp. C7-193-C7-208 ◽  
Author(s):  
F. Penent ◽  
C. Chardonnet ◽  
D. Delande ◽  
F. Biraben ◽  
J. C. Gay

Author(s):  
Fulpagare Priya K. ◽  
Nitin N. Patil

Social Network is an emerging e-service for Content Sharing Sites (CSS). It is an emerging service which provides reliable communication. Some users over CSS affect user’s privacy on their personal contents, where some users keep on sending annoying comments and messages by taking advantage of the user’s inherent trust in their relationship network. Integration of multiple user’s privacy preferences is very difficult task, because privacy preferences may create conflict. The techniques to resolve conflicts are essentially required. Moreover, these methods need to consider how users would actually reach an agreement about a solution to the conflict in order to offer solutions acceptable by all of the concerned users. The first mechanism to resolve conflicts for multi-party privacy management in social media that is able to adapt to different situations by displaying the enterprises that users make to reach a result to the conflicts. Billions of items that are uploaded to social media are co-owned by multiple users. Only the user that uploads the item is allowed to set its privacy settings (i.e. who can access the item). This is a critical problem as users’ privacy preferences for co-owned items can conflict. Multi-party privacy management is therefore of crucial importance for users to appropriately reserve their privacy in social media.


2018 ◽  
Vol 13 (2) ◽  
pp. 187-211
Author(s):  
Patricia E. Chu

The Paris avant-garde milieu from which both Cirque Calder/Calder's Circus and Painlevé’s early films emerged was a cultural intersection of art and the twentieth-century life sciences. In turning to the style of current scientific journals, the Paris surrealists can be understood as engaging the (life) sciences not simply as a provider of normative categories of materiality to be dismissed, but as a companion in apprehending the “reality” of a world beneath the surface just as real as the one visible to the naked eye. I will focus in this essay on two modernist practices in new media in the context of the history of the life sciences: Jean Painlevé’s (1902–1989) science films and Alexander Calder's (1898–1976) work in three-dimensional moving art and performance—the Circus. In analyzing Painlevé’s work, I discuss it as exemplary of a moment when life sciences and avant-garde technical methods and philosophies created each other rather than being classified as separate categories of epistemological work. In moving from Painlevé’s films to Alexander Calder's Circus, Painlevé’s cinematography remains at the forefront; I use his film of one of Calder's performances of the Circus, a collaboration the men had taken two decades to complete. Painlevé’s depiction allows us to see the elements of Calder's work that mark it as akin to Painlevé’s own interest in a modern experimental organicism as central to the so-called machine-age. Calder's work can be understood as similarly developing an avant-garde practice along the line between the bestiary of the natural historian and the bestiary of the modern life scientist.


Author(s):  
. Anika ◽  
Navpreet Kaur

The paper exhibits a formal audit on early detection of heart disease which are the major cause of death. Computational science has potential to detect disease in prior stages automatically. With this review paper we describe machine learning for disease detection. Machine learning is a method of data analysis that automates analytical model building.Various techniques develop to predict cardiac disease based on cases through MRI was developed. Automated classification using machine learning. Feature extraction method using Cell Profiler and GLCM. Cell Profiler a public domain software, freely available is flourished by the Broad Institute's Imaging Platform and Glcm is a statistical method of examining texture .Various techniques to detect cardio vascular diseases.


2009 ◽  
Vol 8 (3) ◽  
pp. 887-897
Author(s):  
Vishal Paika ◽  
Er. Pankaj Bhambri

The face is the feature which distinguishes a person. Facial appearance is vital for human recognition. It has certain features like forehead, skin, eyes, ears, nose, cheeks, mouth, lip, teeth etc which helps us, humans, to recognize a particular face from millions of faces even after a large span of time and despite large changes in their appearance due to ageing, expression, viewing conditions and distractions such as disfigurement of face, scars, beard or hair style. A face is not merely a set of facial features but is rather but is rather something meaningful in its form.In this paper, depending on the various facial features, a system is designed to recognize them. To reveal the outline of the face, eyes, ears, nose, teeth etc different edge detection techniques have been used. These features are extracted in the term of distance between important feature points. The feature set obtained is then normalized and are feed to artificial neural networks so as to train them for reorganization of facial images.


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