57Co-Bleomycin Scintigraphy in Staging of Carcinoma of the Cervix

1985 ◽  
Vol 24 (01) ◽  
pp. 48-51 ◽  
Author(s):  
K. Dursk ◽  
T. Pertyński
Keyword(s):  
Cervical Cancer ◽  
Roc Curves ◽  
Selection Of

Summary65 patients with cervical cancer at various stages of the disease were studied scintigraphically after 57Co-bleomycin administration, active urine being flushed out from the bladder continuously. The patients were submitted to other auxiliary tests and the interpretation of scintigraphic images could thus be verified. Scintigraphic data were classified semiquantitatively by scoring in the range from zero to 5. Results were evaluated in terms of ROC-curves. This method of analysis permitted selection of cut-off levels for binary decisions that yielded satisfactory sensitivity and acceptable specificity of the test.

scholarly journals Development and validation of a sequential two-step algorithm for the screening of individuals with potential polycythaemia vera

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Miguel Piris-Villaespesa ◽  
Alberto Álvarez-Larrán ◽  
Adolfo Saez-Marín ◽  
Claudia Nuñez-Torrón ◽  
Gloria Muñoz-Martin ◽  
...  
Keyword(s):  
Mutation Screening ◽  
Roc Curves ◽  
Polycythaemia Vera ◽  
Normal Ranges ◽  
Screening Algorithm ◽  
Number Of Patients ◽  
Step Algorithm ◽  
Development And Validation ◽  
Diagnostic Criterion ◽  
Selection Of

AbstractIn 2016, the WHO included haemoglobin values within normal ranges as a diagnostic criterion for Polycythaemia Vera (PV). Since then, concerns have arisen that a large number of patients are undergoing unnecessary screening for PV. To address this issue, we estimated the prevalence of JAK2 p.V617F in individuals with elevated haemoglobin or haematocrit and developed and validated a screening algorithm for PV. A total of 15,366 blood counts performed in seven non-consecutive days were reviewed, of which 1001 were selected for subsequent JAK2 p.V617F mutation screening. Eight (0.8%) new JAK2 p.V617F-mutated cases were detected. From ROC curves, a two-step algorithm was developed based on the optimal cut-off for the detection of the JAK2 p.V617F mutation. The algorithm was prospectively validated in an independent cohort of 15,298 blood counts. A total of 1595 (10.4%) cases met the criterion for haemoglobin or haematocrit, of whom 581 passed to step 2 (3.8% of the total). The JAK2 p.V617F mutation was detected in 7 of the 501 patients tested, which accounts for 0.04% of the total cohort and 0.4% of patients with erythrocytosis. In conclusion, this data show that our two-step algorithm improves the selection of candidates for JAK2 p.V617F testing.

scholarly journals The ratio of serum Angiopoietin-1 to Angiopoietin-2 in patients with cervical cancer is a valuable diagnostic and prognostic biomarker

PeerJ ◽  
2017 ◽  
Vol 5 ◽  
pp. e3387 ◽  
Author(s):  
Ping Yang ◽  
Na Chen ◽  
Dongyun Yang ◽  
Janet Crane ◽  
Shouhua Yang ◽  
...  
Keyword(s):  
Cervical Cancer ◽  
Cell Cancer ◽  
Progression Free Survival ◽  
Intraepithelial Neoplasia ◽  
Roc Curves ◽  
Enzyme Linked Immunosorbent Assay ◽  
Advanced Tumor Stage ◽  
Cervical Lesions ◽  
Advanced Tumor ◽  
Angiopoietin 1

Objectives Angiopoietins have been found to play essential roles in tumor angiogenesis. The present study was aimed at investigating the diagnostic and prognostic values of serum angiopoietin 1 and 2 (sAng-1 and sAng-2) in cervical cancer. Methods The sAng-1 and sAng-2 concentrations were analyzed in 77 women with cervical cancer, 44 women with cervical intraepithelial neoplasia (CIN) and 43 women without cervical lesions by enzyme-linked immunosorbent assay. The diagnostic values of sAng-1, sAng-2 and sAng-1/sAng-2 were evaluated by receiver operating characteristic (ROC) curves. The Ang-1 and Ang-2 expression in cervical cancer tissues as well as microvessel density (MVD), were assessed by immunohistochemistry. Results The concentration of sAng-2 gradually increased and the sAng-1/Ang-2 ratio was gradually decreased from normal control to CIN, then to squamous cell cancer, and the sAng-1/sAng-2 ratio was also significantly decreased in adenocarcinoma. The area under ROC curves of sAng-2 and sAng-1/sAng-2 ratio for discriminating cervical cancer from normal were 0.744 and 0.705, respectively. Decreased sAng-1/sAng-2 was significantly associated with advanced tumor stage, poor differentiation, lymph-vascular space invasion and high MVD. sAng-2 was positively correlated with the Ang-2 expression in cervix epithelia. A high sAng-1/sAng-2 ratio was associated with a longer progression-free survival and a longer overall survival in cervical cancer patients. Conclusions These findings suggest that sAng-2 and the sAng-1/sAng-2 ratio may be valuable diagnostic and prognostic biomarkers for cervical cancer.

Perfusion parameters of intravoxel incoherent motion based on tumor edge region of interest in cervical cancer: evaluation of differentiation and correlation with dynamic contrast-enhanced MRI

2019 ◽  
Vol 61 (8) ◽  
pp. 1087-1095
Author(s):  
Hongyan Wang ◽  
Lixia Zhu ◽  
Guohua Li ◽  
Menzhe Zuo ◽  
Xi Ma ◽  
...  
Keyword(s):  
Cervical Cancer ◽  
Region Of Interest ◽  
Roc Curves ◽  
Edge Region ◽  
Dce Mri ◽  
Contrast Enhanced ◽  
Poorly Differentiated ◽  
Positive Correlations ◽  
Tumor Edge ◽  
Contrast Enhanced Mri

Background Intravoxel incoherent motion (IVIM) diffusion-weighted imaging (DWI) is a functional magnetic resonance imaging (MRI) sequence. Purpose To evaluate the value of perfusion parameters derived from IVIM-DWI based on tumor edge region of interest (ROI) in differentiation in cervical cancer and investigate the relationship between IVIM and dynamic contrast-enhanced MRI (DCE-MRI). Material and Methods Thirty-three patients with pathologically diagnosed squamous cell carcinoma who underwent IVIM-DWI (nine b-values: 1–1000 s/mm2) and DCE-MRI were retrospectively assessed in this study. Parameters of IVIM (D, f, D*, fD*) and quantitative parameters of DCE-MRI (Ktrans, Kep, Ve) were derived using tumor edge ROI. Mann–Whitney U test was used to compare parameters between pathological grades and receiver operating characteristic (ROC) curves were used. Pearson’s correlation coefficient (r) evaluated the correlation between perfusion parameters derived from IVIM and DCE-MRI. Results The poorly differentiated group showed the significantly lower D value and the higher f, Ktrans and Kep values than the well-to-moderately differentiated group ( P < 0.05). ROC curves indicated that f < 26%, Ktrans <0.38/min, and Kep <1.62/min could differentiate the poorly differentiated group from the well-to-moderately differentiated group (AUC 0.753–0.808). Significantly positive correlations were found between f and Ktrans (r = 0.422, P = 0.014) and between fD* and Ktrans (r = 0.448, P = 0.009). Conclusion Perfusion parameters derived from IVIM based on tumor edge ROI may offer additional value in differentiation in cervical cancer, and the IVIM perfusion parameters showed moderate positive correlations with quantitative perfusion parameters from DCE-MRI, while f and fD* showed promising significance.

scholarly journals Rapid Antibiotic Combination Testing for Carbapenem-Resistant Gram-Negative Bacteria within Six Hours Using ATP Bioluminescence

2018 ◽  
Vol 62 (9) ◽  
Author(s):  
Yiying Cai ◽  
Celene L. Seah ◽  
Hui Leck ◽  
Tze-Peng Lim ◽  
Jocelyn Q. Teo ◽  
...  
Keyword(s):  
Predictive Accuracy ◽  
Roc Curves ◽  
Gram Negative Bacteria ◽  
Content Type ◽  
Carbapenem Resistant ◽  
Atp Bioluminescence ◽  
Bioluminescence Assay ◽  
Atp Bioluminescence Assay ◽  
Selection Of

ABSTRACTTo guide the timely selection of antibiotic combinations against carbapenem-resistant Gram-negative bacteria (CR-GNB), anin vitrotest with a short turnaround time is essential. We developed anin vitroATP bioluminescence assay to determine effective antibiotic combinations against CR-GNB within 6 h. We tested 42 clinical CR-GNB strains (14Acinetobacter baumannii, 14Pseudomonas aeruginosa, and 14Klebsiella pneumoniaestrains) against 74 single antibiotics and two-antibiotic combinations. Bacteria (approximately 5 log10CFU/ml) were incubated with an antibiotic(s) at 35°C; ATP bioluminescence was measured at 6 h and 24 h; and the measurements were compared to viable counts at 24 h. Receiver operating characteristic (ROC) curves were used to determine the optimal luminescence thresholds (TRLU) for distinguishing between inhibitory and noninhibitory combinations. The areas under the 6-h and 24-h ROC curves were compared using the DeLong method. Prospective validation of the established thresholds was conducted using 18 additional CR-GNB. The predictive accuracy ofTRLUfor the 6-h ATP bioluminescence assay was 77.5% when all species were analyzed collectively. Predictive accuracies ranged from 73.7% to 82.7% when each species was analyzed individually. Upon comparison of the areas under the 6-h and 24-h ROC curves, the 6-h assay performed significantly better than the 24-h assay (P< 0.01). Predictive accuracy remained high upon prospective validation of the 6-h ATP assay (predictive accuracy, 79.8%; 95% confidence interval [CI], 77.6 to 81.9%), confirming the external validity of the assay. Our findings indicate that our 6-h ATP bioluminescence assay can provide guidance for prospective selection of antibiotic combinations against CR-GNB in a timely manner and may be useful in the management of CR-GNB infections.

scholarly journals Antigen-Driven T-Cell Selection in Patients with Cervical Cancer as Evidenced by T-Cell Receptor Analysis and Recognition of Autologous Tumor

2002 ◽  
Vol 9 (2) ◽  
pp. 267-278 ◽  
Author(s):  
H. Pilch ◽  
H. Höhn ◽  
C. Neukirch ◽  
K. Freitag ◽  
P. G. Knapstein ◽  
...  
Keyword(s):  
Cervical Cancer ◽  
T Cell ◽  
T Cell Receptor ◽  
Tumor Tissue ◽  
Cell Receptor ◽  
Autologous Tumor ◽  
Short Term ◽  
Cell Repertoire ◽  
Tcr Repertoire ◽  
Selection Of

ABSTRACT We characterized the T-cell receptor (TCR) repertoire in freshly harvested tumor lesions, in short-term-expanded CD4+ tumor infiltrating lymphocytes (TIL) as well as in CD4+ and CD8+ peripheral blood lymphocytes (PBL) from three patients with cervical cancer. Skewing of the T-cell repertoire as defined by measuring the length of the complementarity-determining region 3 (CDR3) of the TCR VA and VB chains was observed in CD8+ PBL, in freshly harvested tumor tissue, as well as in CD4+ TIL. Comparative analysis of the TCR repertoire revealed unique monoclonal TCR transcripts within the tumor lesion which were not present in PBL, suggesting selection of TCR clonotypes due to antigenic stimulation. TCR repertoire analysis of the short-term (7-day) CD4+ TIL lines revealed that the TCR composition is markedly different from that in CD4+ PBL or in the freshly harvested tumor tissue. Only one-third of CD4+ TIL lines showed HLA-DR-restricted recognition of autologous tumor cells as defined by cytolysis. These data provide support for the antigen-driven selection of T cells within cervical cancer lesions and suggest that analysis of the TCR repertoire may aid in obtaining an objective description of the immune response in patients with cervical cancer who are undergoing epitope-based immunotherapy.

scholarly journals Potentialities of multi-b-values diffusion-weighted imaging for predicting efficacy of concurrent chemoradiotherapy in cervical cancer patients

2020 ◽  
Author(s):  
Bing Liu ◽  
Wan-Ling Ma ◽  
Guang-Wen Zhang ◽  
Zhen Sun ◽  
Meng-Qi Wei ◽  
...  
Keyword(s):  
Cervical Cancer ◽  
Diffusion Coefficient ◽  
Treatment Response ◽  
Cancer Patients ◽  
Predictive Ability ◽  
Roc Curves ◽  
Poor Responders ◽  
Noninvasive Technique ◽  
B Values ◽  
Predict Treatment Response

Abstract Purpose: To testify whether multi-b-values DWI can be used to ultra-early predict treatment response of CCRT in cervical cancer patients and to assess the predictive ability of concerning parameters. Methods: 53 patients with biopsy proved cervical cancer were retrospectively recruited in this study. All patients underwent pelvic multi-b-values DWI before and at the 3rd day during treatment. Tumor diffusion parameters were calculated by mono-exponential (apparent diffusion coefficient, ADC), bi-exponential (Dslow, Dfast and fp) and stretched exponential (distributed diffusion coefficient, DDC and α) models. Treatment response was assessed based on RECIST v1.1 at 1 month after the completion of whole CCRT. Parameters were compared using independent t test or Mann-Whitney U test as appropriate. Receiver operating characteristic (ROC) curves was used for statistical evaluations. Results: ADC-T0 (p=0.02), Dslow-T0 (p<0.01), DDC-T0 (p=0.03), ADC-T1 (p<0.01), Dslow-T1 (p<0.01), ΔADC (p=0.04) and Δα (p<0.01) were significant lower in non-CR group patients. ROC analyses showed that ADC-T1 and Δα exhibited high prediction value, with area under the curves of 0.880 and 0.869, respectively. Conclusion: Multi-b-values DWI can be used as a noninvasive technique to assess and predict treatment response in cervical cancer patients at the 3rd day of CCRT. ADC-T1 and Δα can be used to differentiate good responders from poor responders.

scholarly journals Feature Selection of Non-Dermoscopic Skin Lesion Images for Nevus and Melanoma Classification

Computation ◽  
2020 ◽  
Vol 8 (2) ◽  
pp. 41 ◽  
Author(s):  
Felicia Anisoara Damian ◽  
Simona Moldovanu ◽  
Nilanjan Dey ◽  
Amira S. Ashour ◽  
Luminita Moraru
Keyword(s):  
Fourier Transform ◽  
Area Under The Curve ◽  
Roc Curves ◽  
Noise Removal ◽  
Skin Lesions ◽  
Asymmetry Index ◽  
Color Distribution ◽  
Atypical Feature ◽  
Melanoma Classification ◽  
Selection Of

(1) Background: In this research, we aimed to identify and validate a set of relevant features to distinguish between benign nevi and melanoma lesions. (2) Methods: Two datasets with 70 melanomas and 100 nevi were investigated. The first one contained raw images. The second dataset contained images preprocessed for noise removal and uneven illumination reduction. Further, the images belonging to both datasets were segmented, followed by extracting features considered in terms of form/shape and color such as asymmetry, eccentricity, circularity, asymmetry of color distribution, quadrant asymmetry, fast Fourier transform (FFT) normalization amplitude, and 6th and 7th Hu’s moments. The FFT normalization amplitude is an atypical feature that is computed as a Fourier transform descriptor and focuses on geometric signatures of skin lesions using the frequency domain information. The receiver operating characteristic (ROC) curve and area under the curve (AUC) were employed to ascertain the relevance of the selected features and their capability to differentiate between nevi and melanoma. (3) Results: The ROC curves and AUC were employed for all experiments and selected features. A comparison in terms of the accuracy and AUC was performed, and an evaluation of the performance of the analyzed features was carried out. (4) Conclusions: The asymmetry index and eccentricity, together with F6 Hu’s invariant moment, were fairly competent in providing a good separation between malignant melanoma and benign lesions. Also, the FFT normalization amplitude feature should be exploited due to showing potential in classification.

Value of PET-CT in Avoiding Multimodality Therapy in Operable Cervical Cancer

2010 ◽  
Vol 20 (6) ◽  
pp. 1041-1045 ◽  
Author(s):  
Bhupesh K. Goyal ◽  
Harkirat Singh ◽  
Krishan Kapur ◽  
Bhupinder S. Duggal ◽  
Mattakarottu J. Jacob
Keyword(s):  
Cervical Cancer ◽  
Multimodality Treatment ◽  
Nodal Metastasis ◽  
Multimodality Therapy ◽  
Emission Tomography ◽  
Nodal Disease ◽  
Adjuvant Chemoradiation ◽  
Positron Emission ◽  
Pet Ct ◽  
Selection Of

Background:Posttreatment morbidity of multimodality therapy is substantially higher than either radical surgery or chemoradiation alone. Preoperative prediction of positive nodes limits optimal selection of the primary treatment modality. Computed tomography (CT) and magnetic resonance imaging have been tried for nodal assessment with modest results. Positron emission tomography (PET) combined with CT seems to be promising in this regard. This clinicopathologic study assesses the value of PET-CT in predicting nodal metastasis and avoiding multimodality therapy.Materials and Methods:Eighty patients with clinically operable cervical cancer underwent PET-CT during the preoperative evaluation followed by radical hysterectomy. Adjuvant chemoradiation was administered when indicated by histopathologic findings. The histopathologic finding of the pelvic nodes was correlated with the PET-CT findings for nodal metastasis. The χ2 test was used as the test of significance in the statistical analysis.Observations:Of 62 patients found PET-CT negative for nodal disease, 52 were true negative, whereas 10 were false negative on histopathologic examination. On the other hand, 14 of 18 patients found PET-CT positive for nodal disease were true positives. Specificity, sensitivity, positive predictive value, and negative predictive value of PET-CT in nodal assessment were found to be 92.8, 58.33, 77.7, and 83.8, respectively. Twenty-four patients (30%) with pelvic nodes positive for disease on histopathologic examination were administered adjuvant chemoradiation.Had we operated only on those patients who were PET-CT negative for nodal disease, 10 of 62 patients would have required adjuvant chemoradiation for positive nodes. Eighteen patients found PET-CT positive for nodal disease would be treated with primary chemoradiation. Inclusion of PET-CT in the decision-making process for primary surgery versus primary chemoradiation would allow 87.5% patients to receive a single modality of treatment (65%, only surgery; 22.5%, only chemoradiation) and the proportion of patients requiring multimodality treatment would reduce significantly from 30% to 12.5% (P < 0.01).Conclusion:Positron emission tomography combined with CT in the evaluation of operable cervical cancer can help in the optimal selection of patients for surgery such that multimodality treatment with its attendant increase in morbidity is avoided.

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