Bioartificial bone grafting:Tarsal joint fusion in a dog using a bioartificial composite bone graft consisting of β-tricalciumphosphate and platelet rich plasma – A case report

2005 ◽  
Vol 18 (01) ◽  
pp. 52-54 ◽  
Author(s):  
H.-A. Merten ◽  
A. Bader ◽  
G. Uhr ◽  
A. Deivick ◽  
A. Meyer-Lindenberg ◽  
...  
Keyword(s):  
Bone Graft ◽  
Platelet Rich Plasma ◽  
Healing Process ◽  
Tumor Resection ◽  
Experimental Studies ◽  
Tarsal Joint ◽  
Intertarsal Joint ◽  
The Right ◽  
Large Bone ◽  
Large Bone Defects

SummaryGrafting of large bone defects caused by trauma or tumor resection still remains a problem to solve. In experimental studies as well as in human dentistry, osteoconduction and biodegradation of the β-tricalciumphosphate Cerasorb® as well as osteoinductive capabilities of platelet rich plasma have been proven. In case of luxation of the right tarsal joint, including a compressive fracture of the forth tarsal bone, the recommended use of autologous cancellous bone to support osseous fusion was replaced by the use of a bioartificial bone graft. Biodegradation of the bone graft was proven clinically and radiographically. Complete osseous fusion of the intertarsal joint occurred. The use of the β-tricalciumphosphate Cerasorb in combination with platelet rich plasma to support a partial arthrodesis of the tarsal joint in a dog did not impede the healing process but led to full recovery of the patient, indicating that this concept of bioartificial bone grafting could support bone healing.

Clinical Efficacy of Bone Reconstruction Surgery with Frozen Cortical Bone Allografts for Nonunion of Radial and Ulnar Fractures in Toy Breed Dogs

2018 ◽  
Vol 31 (03) ◽  
pp. 159-169
Author(s):  
Yukari Nagahiro ◽  
Daichi Katori ◽  
Norihiro Muroi ◽  
Hiroyuki Akagi ◽  
Nobuo Kanno ◽  
...  
Keyword(s):  
Cortical Bone ◽  
Bone Remodelling ◽  
Weight Bearing ◽  
Healing Process ◽  
Bone Reconstruction ◽  
Bone Allografts ◽  
Bone Remodelling Process ◽  
Large Bone ◽  
Atrophic Nonunion ◽  
Large Bone Defects

Objective To evaluate the effectiveness of frozen cortical bone allografts (FCBA) in the treatment of severe radial and ulnar atrophic nonunion fractures. Animals Toy breed dogs with nonunion of radial and ulnar fractures (n = 15). Methods Severe atrophic nonunion fractures were treated with FCBA (eight infected and seven non-infected fractures). Radiographs obtained immediately after surgery, and 1, 2, 3, 6 and 12 months later were evaluated and scored for the periosteal reaction at the bone regeneration sites, the healing process in the bone connection areas at both the proximal and distal sites, and the bone remodelling process within the allografts. Results Improvements in the fracture-healing process and weight-bearing function were observed in all cases. Radiographic scores at the bone connection areas and within the allograft improved significantly over time (p < 0.05). There were not any significant differences in radiographic scores between the infected and non-infected groups. Clinical Significance Bone reconstruction with FCBA is effective in the treatment of radial and ulnar nonunion fractures associated with large bone defects, regardless of the infection status of the surgical site.

Reconstruction of Large Bone Defects and Complex Non-Unions Using a Free Fibular Bone Graft and a Supplementary Allograft

2020 ◽  
Author(s):  
Selina Gaida ◽  
Uwe Schweigkofler ◽  
Wibke Moll ◽  
Michael Sauerbier ◽  
Reinhard Hoffmann
Keyword(s):  
Bone Graft ◽  
Case Series ◽  
Bone Defects ◽  
Bone Transplantation ◽  
Patient Comfort ◽  
Masquelet Technique ◽  
Specialized Center ◽  
Free Fibula ◽  
Large Bone ◽  
Large Bone Defects

AbstractLarge bone defects or complex pseudarthrosis represent an interdisciplinary challenge. Established surgical procedures include autogenous cancellous bone graft, the Masquelet technique or bone transfer via segment transport as well as free microvascular bone transplantation. However, the successful use of all these techniques requires a specialized center with great interdisciplinary expertise. In the following case series we describe the technique of free fibula transplantation and additional allograft. In both cases a good functional result with full mechanical strength of the affected extremity and satisfactory patient comfort has been achieved. In the second case, implant failure with the necessity of revision endoprosthetics occurred during the procedure.

Bone Transport Osteogenesis for Limb Salvage

2000 ◽  
Vol 13 (01) ◽  
pp. 18-22 ◽  
Author(s):  
M. Degna ◽  
A. Feretti ◽  
P. Buracco ◽  
Nicole Ehrhart
Keyword(s):  
Surgical Technique ◽  
Limb Salvage ◽  
Tumor Resection ◽  
Bone Defects ◽  
Bone Transport ◽  
Successful Outcome ◽  
Strict Adherence ◽  
Initial Learning ◽  
Large Bone ◽  
Large Bone Defects

Six dogs with osteosarcoma of the radius were treated by local resection and limb salvage using bone transport osteogenesis. One case is described in detail. Although the initial learning curve is steep for this technique, strict adherence to oncologic surgical technique and the principles of Ilizarov can lead to a successful outcome. Bone transport shows promise as an alternative to cortical allografts for reconstruction of large bone defects after tumor resection in the canine radius.

Evaluation of the Bone Healing Process Utilizing Platelet-Rich Plasma Activated by Thrombin and Calcium Chloride: A Histologic Study in Rabbit Calvaria

2013 ◽  
Vol 39 (1) ◽  
pp. 14-21 ◽  
Author(s):  
Walter Betoni-Junior ◽  
Paula Dechichi ◽  
Jônatas Caldeira Esteves ◽  
Darceny Zanetta-Barbosa ◽  
Aparecido Eurípedes Onório Magalhães
Keyword(s):  
Bone Graft ◽  
Calcium Chloride ◽  
Bone Healing ◽  
Bone Repair ◽  
Platelet Rich Plasma ◽  
Healing Process ◽  
Bone Defects ◽  
Histomorphometric Analysis ◽  
Histologic Study ◽  
Vascularized Bone

To evaluate the bone healing of defects filled with particulate bone graft in combination with platelet-rich plasma (PRP), added with a mixture of calcium chloride and thrombin or just calcium chloride. Two 5-mm bone defects were created in the calvaria of 24 rabbits. Each defect was filled with particulate bone graft and PRP. In one defect the PRP was activated by a mixture of calcium chloride and thrombin; in the other, PRP was activated by calcium chloride only. The animals were euthanized 1, 2, 4, and 8 weeks after the surgeries, and the calvaria was submitted to histologic processing for histomorphometric analysis. The qualitative analysis has shown that both defects presented the same histologic characteristics so that a better organized, more mature, and well-vascularized bone tissue was noticed in the eighth week. A good bone repair was achieved using either the mixture of calcium chloride and thrombin or the calcium chloride alone as a restarting agent of the coagulation process.

scholarly journals Benefit of Leukocyte- and Platelet-Rich Plasma in Operative Wound Closure in Oral and Maxillofacial Surgery

2016 ◽  
Vol 2016 ◽  
pp. 1-5 ◽  
Author(s):  
Agata Cieślik-Bielecka ◽  
Justyna Glik ◽  
Rafał Skowroński ◽  
Tomasz Bielecki
Keyword(s):  
Oral Mucosa ◽  
Platelet Rich Plasma ◽  
Healing Process ◽  
Maxillofacial Surgery ◽  
Mandibular Fracture ◽  
Control Group ◽  
Oral And Maxillofacial Surgery ◽  
Mandibular Fractures ◽  
The Right ◽  
Healing Processes

This article reports the influence of an autologous leukocyte- and platelet-rich plasma (L-PRP) injection as a minimally invasive method on supporting wound healing processes after a mandibular odontogenic cystectomy and double mandibular fracture fixation. 113 patients were enrolled into a control group (received no L-PRP injection) and 102 patients were enrolled into an L-PRP group with an oral mucosa incision. 18 patients after a double mandibular fracture were operated on using 2 external submandibular approaches receiving no fluids in the right site (a control group) and an L-PRP injection in the left incision (L-PRP group). Clinical observations showed that the oral mucosa healed faster in patients treated with L-PRP, in comparison to cases where inductive biomaterial was not added. Pain at the L-PRP injection site was relieved within few hours after an operation in patients with double mandibular fractures. However, there were no differences observed in the progression of the healing process. L-PRP possesses inductive properties that could stimulate healing processes and it seems to be one of the most promising methods in the future for the treatment of soft tissue defects.

scholarly journals An Innovative Bioceramic Bone Graft with Platelet-Rich Plasma for Rapid Bone Healing and Regeneration in a Rabbit Model

2021 ◽  
Vol 11 (11) ◽  
pp. 5271
Author(s):  
Syamsiah Syam ◽  
Chun-Wei Chang ◽  
Wen-Chien Lan ◽  
Keng-Liang Ou ◽  
Bai-Hung Huang ◽  
...  
Keyword(s):  
Bone Graft ◽  
Maxillary Sinus ◽  
Bone Healing ◽  
Platelet Rich Plasma ◽  
Rabbit Model ◽  
Graft Material ◽  
Computed Tomographic ◽  
Hematoxylin And Eosin ◽  
The Right

This study aimed to investigate the effect of combining an innovative bioceramic α-calcium sulfate hemihydrate (α-CSH, CaSO4⋅0.5H2O) bone graft and platelet-rich plasma (PRP) to accelerate bone healing and regeneration in a rabbit model. The bone graft material was implanted bilaterally on rabbit’s artificially maxillary sinus defects: the right maxillary sinus received α-CSH, while α-CSH combine with PRP (α-CSH/PRP) was grafted in left site. The quantity and quality of bone formation after implantation were analyzed radiographically and histologically at 1, 2, and 3 weeks. The micro-computed tomographic results indicated that the bone density of sinus implanted with α-CSH increased and defect volume decreased most after 2 weeks. In histological analysis, both hematoxylin and eosin and Masson trichrome staining of α-CSH/PRP displays better bone healing and regeneration progress than α-CSH after 2 weeks implantation. Therefore, the innovative α-CSH combined with PRP was revealed to be useful in accelerating bone healing and regeneration for the successful defect treatment.

scholarly journals Healing of Large Segmental Bone Defect after Implantation of Autogenous Cancellous Bone Graft in Comparison to Hydroxyapatite and 0.5% Collagen Scaffold Combined with Mesenchymal Stem Cells

2010 ◽  
Vol 79 (4) ◽  
pp. 607-612 ◽  
Author(s):  
Alois Nečas ◽  
Pavel Proks ◽  
Lucie Urbanová ◽  
Robert Srnec ◽  
Ladislav Stehlík ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Bone Graft ◽  
Cancellous Bone ◽  
Bone Defects ◽  
Cancellous Bone Graft ◽  
Collagen Group ◽  
Significant Difference ◽  
Large Bone ◽  
Large Bone Defects

At present, attention is focused on research into possibilities of healing large bone defects by the method of mini-invasive osteosynthesis, using implantation of biomaterials and mesenchymal stem cells (MSCs). This study evaluates the healing of segmental femoral defects in miniature pigs based on the radiological determination of the callus: cortex ratio at 16 weeks after ostectomy. The size of the formed callus was significantly larger (p < 0.05) in animals after transplantation of an autogenous cancellous bone graft (group A, callus : cortex ratio of 1.77 ± 0.33) compared to animals after transplantation of cylindrical scaffold from hydroxyapatite and 0.5% collagen (group S, callus : cortex ratio of 1.08 ± 0.13), or in animals after transplantation of this scaffold seeded with MSCs (group S + MSCs, callus: cortex ratio of 1.15 ± 0.18). No significant difference was found in the size of callus between animals of group S and animals of group S + MSCs. Unlike a scaffold in the shape of the original bone column, a freely placed autogenous cancellous bone graft may allow the newly formed tissue to spread more to the periphery of the ostectomy defect. Implanted cylindrical scaffolds (with and without MSCs) support callus formation directly in the center of original bone column in segmental femoral ostectomy, and can be successfully used in the treatment of large bone defects.

scholarly journals Immunohistochemical Expression of Collagens in the Skin of Horses Treated with Leukocyte-Poor Platelet-Rich Plasma

2015 ◽  
Vol 2015 ◽  
pp. 1-12 ◽  
Author(s):  
Maria Verônica de Souza ◽  
Mariana Brettas Silva ◽  
José de Oliveira Pinto ◽  
Marianna Barros de Souza Lima ◽  
Júlio Crepaldi ◽  
...  
Keyword(s):  
Platelet Rich Plasma ◽  
Normal Skin ◽  
Healing Process ◽  
Treated Group ◽  
Type I ◽  
Immunohistochemical Expression ◽  
Reticular Dermis ◽  
Wound Induction ◽  
Three Samples ◽  
The Right

This study evaluated the immunohistochemical expression of type I (COL I) and III (COL III) collagens during the healing process of skin treated with leukocyte-poor platelet-rich plasma (LP-PRP). Seven healthy gelding crossbred horses aged 16 to 17 years were used. Two rectangle-shaped wounds were created surgically in the right and left gluteal regions. Twelve hours after wound induction, 0.5 mL of the LP-PRP was administered in each edge of the wounds of one of the gluteal regions. The contralateral region was used as control (CG). Three samples were obtained: after wound induction (T0), 14 days (T1) of healing process, and after complete closure of the skin (T2). The normal skin (T0) showed strong staining for type III and I collagen in papillary and reticular dermis, respectively. In the scar of the treated group, COL III showed important (p<0.05) increase in immunoreaction in T2 compared with T1. The administration of a single dose of LP-PRP 12 h after induction of wound in horses does not influence formation of collagens I and III. However, the intense labeling for COL III suggests that the tissue was still weak during the macroscopic closure of the wound, demonstrating that healing was not completely finished.

Reconstruction of Large Bone Defect after Bone and Soft Tissue Tumor Resection , using Jeat - Treated Autogenous Isotrophic Bone Graft

1995 ◽  
Vol 30 (5) ◽  
pp. 1308
Author(s):  
Jong Seok Lee ◽  
Dae Geun Jeon ◽  
Ha Yong Kim ◽  
Yong Hyeog Kang ◽  
Dong Hwan Chung ◽  
...  
Keyword(s):  
Soft Tissue ◽  
Bone Graft ◽  
Bone Defect ◽  
Soft Tissue Tumor ◽  
Tumor Resection ◽  
Large Bone Defect ◽  
Tissue Tumor ◽  
Large Bone

Application of structural allogenous bone graft in two-stage exchange arthroplasty for knee periprosthetic joint infection: a case control study

2021 ◽  
Author(s):  
ChiehAn Chuang ◽  
Sheng-Hsun Lee ◽  
Chih-Hsiang Chang ◽  
Chih-Chien Hu ◽  
Hsin-Nung Shih ◽  
...  
Keyword(s):  
Survival Rate ◽  
Bone Graft ◽  
Joint Infection ◽  
Bone Defects ◽  
Control Group ◽  
Study Group ◽  
Free Survival ◽  
Fresh Frozen ◽  
Large Bone ◽  
Large Bone Defects

Abstract Background: Knee prosthetic joint infection (PJI) is a common but devastating complication after knee arthroplasty. The revision surgeries for knee PJI may become more challenging when it is associated with large bone defects. The application of structural bone allograft in knee revision surgeries with large bone defects is not a new technique. However, there is a lack of literature reporting its efficacy in PJI cases. This study aimed to investigate the outcome of structural fresh frozen allogenous bone grafts in treating patients in knee PJI with large bone defects. Methods: We performed a retrospective cohort analysis of knee PJI cases treated with two-stage exchange arthroplasty at our institution from 2010 to 2016. 12 patients with structural allogenous bone graft reconstructions were identified as the study group. 24 patients without structural allograft reconstructions matched with the study group by age, gender, and Charlson comorbidity index were enrolled as the control group. The functional outcome of the study group was evaluated with the Knee Society Score (KSS). Treatment success was assessed according to the Delphi-based consensus definition. The infection relapse rate and implant survivorship were compared between groups. Results: Revision knees with structural allograft presented excellent improvement in the KSS (33.1 to 75.4). There was no significant difference between infection relapse-free survival rate and prosthesis survival rate in two groups. The 8-year prosthesis survival rate was 90.9% in the study group and 91% in the control group (p = 0.913). The 8-year infection relapse-free survival rate was 80% and 83.3% in the study group and control group, respectively (p = 0.377). Conclusion: The structural fresh frozen allogenous bone graft provided an effective way for bone defect reconstruction in knee PJI with accountable survival rate. Meanwhile, using structural allografts did not increase the relapse rate of infection.

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