INCREASE IN MEGAKARYOCYTE SIZE AND PLOIUY PRECEDES ACCELERATION OF ATHEROGENESIS IN THE HYPERCHOLESTEROLAEMIC RABBIT

Mapping Intimacies ◽

10.1055/s-0038-1643412 ◽

1987 ◽

Author(s):

S Dalby Kristensen ◽

K M Roberts ◽

J F Martin

Keyword(s):

Platelet Count ◽

Growth Factor ◽

Dna Content ◽

Mean Platelet Volume ◽

High Cholesterol Diet ◽

Cholesterol Diet ◽

High Cholesterol ◽

Platelet Volume ◽

Total Size ◽

High Concentrations

Platelet derived growth factor(s) probably synthesized by the megakaryocyte are important in atherogenesis. In a pilot study destruction of the circulating platelets was induced by injection of goat serum containing a specific platelet antibody (APS) to rabbits fed a high cholesterol diet (2g per day) for 12 weeks. Seven days after APS the percentage of atheroma in the aorta measured by planimetry was increased in these animals compared to control animals on the same diet that had been injected with goat serum (GS) 7 days before. In a new study 15 pairs of male litter mate rabbits on high cholesterol diet were randomised in pairs to treatment with either APS or GS. Five pairs of animals were killed 18 hours after the injection, 5 pairs 4 days after and 5 pairs 7 days after the injection and the platelet count, mean platelet volume, megakaryocyte nuclear, cytoplasmic and total size, megakaryocyte DNA distribution and the percentage of atheroma in the aorta were measured. Comparison of these variables between the 2 groups revealed the following statistically significant findings (p<0.05) : 18 hours after the injection the platelet count was decreased and the mean platelet volume increased in the APS group. At day 4 the platelet count, megakaryocyte nuclear, cytoplasmic and total size and the megakaryocyte DNA content were increased in the APS group. At day 7 the platelet count and the percentage of the atheroma were higher in the APS group. Since platelets produced by big megakaryocytes with high DNA content are more reactive than normal platelets, we suggest that the acceleration of atheroma formation demonstrated 7 days after APS is caused by the large number of platelets with possible high concentrations of growth factor(s) derived from the large megakaryocytes with increased DNA content.

Download Full-text

Mangrove (Rhizophora sp) Fruit Extract Inhibits Tumor Growth Factor (TGF)-β1 Expression in High Cholesterol Diet-Fed Rats

Jurnal Farmasi Galenika (Galenika Journal of Pharmacy) ◽

10.22487/j24428744.2021.v7.i3.15667 ◽

2021 ◽

Vol 7 (3) ◽

pp. 213-220

Author(s):

Muhammad Yulis Hamidy ◽

Huriatul Masdar ◽

Winarto

Keyword(s):

Growth Factor ◽

Tumor Growth ◽

Normal Control ◽

Atherogenic Diet ◽

High Cholesterol Diet ◽

Cholesterol Diet ◽

High Cholesterol ◽

Fruit Extract ◽

Tumor Growth Factor ◽

Tgf Β1

Background: Atherosclerosis is a major finding in cardiovascular disease. One of the pro-fibrotic cytokines that play an important role in the atherosclerosis process is Tumor Growth Factor (TGF)-β1, where the presence of high TGF-β1 secretion due to hypercholesterolemia will trigger excessive collagen matrix formation. Objective: To analyze Rhizophora sp fruit extract effect on TGF-β1 expression in high cholesterol diet-fed rats. Material and Methods: Eighteen 10-week-old rats weighing 150-200 g were used in this study. These animals were grouped into 3 groups, each consisting of 6 rats. Group A (normal control) is a group of rats that received a normal diet. Group B (atherogenic control) received a diet that induces atherosclerosis (atherogenic diet). This diet contains 2% cholesterol, 5% goat fat, 0.2% cholic acid and standard diet up to 100%. Atherogenic diet was given for 3 days, and on the first day this group also received vitamin D3 700,000 IU/kg. Group C (treated), apart from receiving an atherogenic diet, was also given Rhizophora sp fruit extract 500 mg/kg body weight. The Rhizophora sp fruit extract effect on TGF-β1 expression was evaluated by immunohistochemical procedure. The area of the expression is calculated using the ImageJ. Results: The results of this study indicate that the expression of TGF-β1 is higher in the group receiving the atherogenic diet than the normal control group (17.3 vs. 8.9; P=0.000). Rhizophora sp fruit extract reduced this expression remarkably (17.3 vs. 11.4; P=0.001). Conclusion: Rhizophora sp fruit extract inhibits the the expression of TGF-β1 in high cholesterol diet-fed rats.

Download Full-text

Pengaruh Lama Pemberian Diet Tinggi Kolesterol terhadap Kadar LDL dan TGF-Β Serum Tikus Putih (Rattus novergicus) strain Wistar

Jurnal Kesehatan Andalas ◽

10.25077/jka.v5i1.481 ◽

2016 ◽

Vol 5 (1) ◽

Author(s):

Biomechy Oktomalioputri ◽

Eryati Darwin ◽

Eva Decroli

Keyword(s):

Growth Factor ◽

Transforming Growth Factor ◽

Low Density Lipoprotein ◽

Density Lipoprotein ◽

Transforming Growth Factor Β ◽

Control Group ◽

High Cholesterol Diet ◽

Low Density ◽

Cholesterol Diet ◽

High Cholesterol

AbstrakDiet tinggi kolesterol ini akan meningkatkan kadar Low Density Lipoprotein (LDL) sebagai penanda hiperlipidemia yang berdampak pada terjadinya aterosklerosis. Transforming Growth Factor β (TGF-β) memiliki peranan dalam proses terjadinya aterosklerosis ini. Keterlibatannya dalam hiperlipidemia sebagai faktor risiko utama aterosklerosis belum banyak diketahui. Tujuan penelitian ini adalah menentukan pengaruh lama permberian diet tinggi kolesterol terhadap kadar LDL dan TGF-β pada tikus putih (Rattus novergicus) strain Wistar. Penelitian ini menggunakan metode post test only control group design yang dilakukan terhadap tikus Rattus novergicus jantan umur 3-4 bulan, berat 200-250 gram. Sampel penelitian terdiri dari 24 ekor tikus yang dibagi menjadi 4 kelompok, yaitu kelompok kontrol, A, B dan C. Selain kelompok kontrol, kelompok tikus diberi diet tinggi kolesterol berupa lemak kambing 10%, telur puyuh 5%, selama 10 hari untuk kelompok A, 20 hari untuk kelompok B dan 30 hari untuk kelompok C. Pada akhir percobaan darah tikus diambil dan dilakukan pemeriksaan kadar LDL dan TGF-β serum. Hasil penelitian diolah secara bivariat. Analisis yang digunakan yaitu uji oneway Anova. Hasil penelitian diketahui terdapat pengaruh lama pemberian diet tinggi kolesterol terhadap peningkatan kadar LDL serum tikus dengan p=0,01 (p<0,05). Terdapat pengaruh lama pemberian diet tinggi kolesterol terhadap penurunan kadar TGF-β dimana p=0,04 (p>0,05). Penelitian ini menyimpulkan bahwa terdapat pengaruh lama pemberian diet tinggi kolesterol terhadap kadar LDL dan tikus putih Rattus novergicus strain Wistar.Kata kunci: diet tinggi kolesterol, LDL, TGF-β AbstractHigh-cholesterol diet will increase Low Density Lipoprotein (LDL) levels which impact to atherosclerosis. Transforming Growth Factor β (TGF-β) play a role in atherosclerosis process. But its involvement in hyperlipidemia as the main risk factor of atherosclerosis still unknown. The objective of this study was to observe the effect of duration of giving high-cholesterol diet on Low Density Lipoprotein and Transforming Growth Factor β levels white rats (Rattus novergicus) Wistar strain. This study uses post-test only control group design, carried out on male rats Rattus novergicus 3-4 months, weight 200-250 grams. The study sample consisted of 24 rats were divided into 4 groups: control group, group A, group B and group C. Except the control group, the group of rats given a high-cholesterol diet in the form of goat fat 10%, 5% quail eggs for 10 days to group A, group B for 20 days and 30 days for group C. At the end of the experiment blood was taken and examined LDL and TGF-β levels. Results were analyzed using bivariate. The analysis is oneway Anova test. The results of research known to have effect of duration on giving high-cholesterol diet to increase serum LDL levels of rats with p = 0.01 (p<0.05). This is the same case with TGF-β, which there was effect of duration on giving high-diet cholesterol diet to decrease serum TGF-β levels of rats where p = 0.04 (p>0.05). This study concludes that there are effect of duration on giving high-cholesterol diet to levels of LDL and TGF-β white rats Rattus novergicus Wistar strain.Keywords: high-cholesterol diet, LDL, TGF β

Download Full-text

Acute and Chronic Changes in Platelet Volume and Count After Cardiopulmonary Bypass Induced Thrombocytopenia in Man

Thrombosis and Haemostasis ◽

10.1055/s-0038-1651061 ◽

1987 ◽

Vol 57 (01) ◽

pp. 55-58 ◽

Cited By ~ 37

Author(s):

J F Martin ◽

T D Daniel ◽

E A Trowbridge

Keyword(s):

Platelet Count ◽

Mean Platelet Volume ◽

Artery Bypass ◽

Bypass Graft ◽

Control Group ◽

Artery Bypass Graft ◽

Platelet Volume ◽

Young Males ◽

The Mean

SummaryPatients undergoing surgery for coronary artery bypass graft or heart valve replacement had their platelet count and mean volume measured pre-operatively, immediately post-operatively and serially for up to 48 days after the surgical procedure. The mean pre-operative platelet count of 1.95 ± 0.11 × 1011/1 (n = 26) fell significantly to 1.35 ± 0.09 × 1011/1 immediately post-operatively (p <0.001) (n = 22), without a significant alteration in the mean platelet volume. The average platelet count rose to a maximum of 5.07 ± 0.66 × 1011/1 between days 14 and 17 after surgery while the average mean platelet volume fell from preparative and post-operative values of 7.25 ± 0.14 and 7.20 ± 0.14 fl respectively to a minimum of 6.16 ± 0.16 fl by day 20. Seven patients were followed for 32 days or longer after the operation. By this time they had achieved steady state thrombopoiesis and their average platelet count was 2.44 ± 0.33 × 1011/1, significantly higher than the pre-operative value (p <0.05), while their average mean platelet volume was 6.63 ± 0.21 fl, significantly lower than before surgery (p <0.001). The pre-operative values for the platelet volume and counts of these patients were significantly different from a control group of 32 young males, while the chronic post-operative values were not. These long term changes in platelet volume and count may reflect changes in the thrombopoietic control system secondary to the corrective surgery.

Download Full-text