scholarly journals Mangrove (Rhizophora sp) Fruit Extract Inhibits Tumor Growth Factor (TGF)-β1 Expression in High Cholesterol Diet-Fed Rats

2021 ◽  
Vol 7 (3) ◽  
pp. 213-220
Author(s):  
Muhammad Yulis Hamidy ◽  
Huriatul Masdar ◽  
Winarto
Keyword(s):  
Growth Factor ◽  
Tumor Growth ◽  
Normal Control ◽  
Atherogenic Diet ◽  
High Cholesterol Diet ◽  
Cholesterol Diet ◽  
High Cholesterol ◽  
Fruit Extract ◽  
Tumor Growth Factor ◽  
Tgf Β1

Background: Atherosclerosis is a major finding in cardiovascular disease. One of the pro-fibrotic cytokines that play an important role in the atherosclerosis process is Tumor Growth Factor (TGF)-β1, where the presence of high TGF-β1 secretion due to hypercholesterolemia will trigger excessive collagen matrix formation. Objective: To analyze Rhizophora sp fruit extract effect on TGF-β1 expression in high cholesterol diet-fed rats. Material and Methods: Eighteen 10-week-old rats weighing 150-200 g were used in this study. These animals were grouped into 3 groups, each consisting of 6 rats. Group A (normal control) is a group of rats that received a normal diet. Group B (atherogenic control) received a diet that induces atherosclerosis (atherogenic diet). This diet contains 2% cholesterol, 5% goat fat, 0.2% cholic acid and standard diet up to 100%. Atherogenic diet was given for 3 days, and on the first day this group also received vitamin D3 700,000 IU/kg. Group C (treated), apart from receiving an atherogenic diet, was also given Rhizophora sp fruit extract 500 mg/kg body weight. The Rhizophora sp fruit extract effect on TGF-β1 expression was evaluated by immunohistochemical procedure. The area of ​​the expression is calculated using the ImageJ. Results: The results of this study indicate that the expression of TGF-β1 is higher in the group receiving the atherogenic diet than the normal control group (17.3 vs. 8.9; P=0.000). Rhizophora sp fruit extract reduced this expression remarkably (17.3 vs. 11.4; P=0.001). Conclusion: Rhizophora sp fruit extract inhibits the the expression of TGF-β1 in high cholesterol diet-fed rats.

scholarly journals The Effect of Aqueous Extract of Vernonia Amygdalina (Bitter Leaf) on Antioxidants and the Liver of Rabbits on High Cholesterol Diet

2020 ◽  
pp. 91-100
Author(s):  
O. Abdulmalik ◽  
O. O. Oladapo
Keyword(s):  
Superoxide Dismutase ◽  
Aqueous Extract ◽  
Fatty Infiltration ◽  
Atherogenic Diet ◽  
Normal Diet ◽  
High Cholesterol Diet ◽  
Aspartate Transaminase ◽  
Cholesterol Diet ◽  
High Cholesterol ◽  
Significant Difference

Aims: To determine the effect of V. amygdalina on the levels of antioxidants and the liver of rabbits fed on a atherogenic diet. Study Design: Prospective experimental study. Place and Duration of study: Study lasted 14 weeks at Department of Anatomy, University of Ibadan, Nigeria. Methodology: Eighteen male rabbits were randomly divided into three groups comprising control, high cholesterol diet and high cholesterol diet plus 200mg/kg of aqueous extract of V. amygdalina. The animals were fed a normal animal chow, or a diet supplemented by 0.5% cholesterol and 1% methionine for twelve weeks. Subsequently, serum was obtained for liver function tests. Samples of the liver of the animals were obtained for antioxidant tests and histology. Results: Aspartate transaminase was significantly elevated in atherogenic diet only group compared to animals on normal diet. There was also no statistically significant difference across the three groups in the alanine transaminase values. Superoxide dismutase were significantly reduced in animals that were administered extract. Malondialdehyde was not statistically different across the three groups. The histology of the liver of the animals on high cholesterol diet (with and without extract administration) revealed similar microscopic presence of fatty infiltration of the liver. Conclusion: The use of high cholesterol diet for a duration of twelve weeks in rabbits resulted in significant increased relative liver weight, aspartate transaminase, superoxide dismutase and hepatic microvesicular steatosis. Furthermore, the use of 200mg/kg of aqueous extract of V. amygdalina had ameliorative effect on the level of superoxide dismutase. It did not have a measurable effect on the malondialdehyde, liver enzymes and liver histology.

INCREASE IN MEGAKARYOCYTE SIZE AND PLOIUY PRECEDES ACCELERATION OF ATHEROGENESIS IN THE HYPERCHOLESTEROLAEMIC RABBIT

1987 ◽  
Author(s):  
S Dalby Kristensen ◽  
K M Roberts ◽  
J F Martin
Keyword(s):  
Platelet Count ◽  
Growth Factor ◽  
Dna Content ◽  
Mean Platelet Volume ◽  
High Cholesterol Diet ◽  
Cholesterol Diet ◽  
High Cholesterol ◽  
Platelet Volume ◽  
Total Size ◽  
High Concentrations

Platelet derived growth factor(s) probably synthesized by the megakaryocyte are important in atherogenesis. In a pilot study destruction of the circulating platelets was induced by injection of goat serum containing a specific platelet antibody (APS) to rabbits fed a high cholesterol diet (2g per day) for 12 weeks. Seven days after APS the percentage of atheroma in the aorta measured by planimetry was increased in these animals compared to control animals on the same diet that had been injected with goat serum (GS) 7 days before. In a new study 15 pairs of male litter mate rabbits on high cholesterol diet were randomised in pairs to treatment with either APS or GS. Five pairs of animals were killed 18 hours after the injection, 5 pairs 4 days after and 5 pairs 7 days after the injection and the platelet count, mean platelet volume, megakaryocyte nuclear, cytoplasmic and total size, megakaryocyte DNA distribution and the percentage of atheroma in the aorta were measured. Comparison of these variables between the 2 groups revealed the following statistically significant findings (p<0.05) : 18 hours after the injection the platelet count was decreased and the mean platelet volume increased in the APS group. At day 4 the platelet count, megakaryocyte nuclear, cytoplasmic and total size and the megakaryocyte DNA content were increased in the APS group. At day 7 the platelet count and the percentage of the atheroma were higher in the APS group. Since platelets produced by big megakaryocytes with high DNA content are more reactive than normal platelets, we suggest that the acceleration of atheroma formation demonstrated 7 days after APS is caused by the large number of platelets with possible high concentrations of growth factor(s) derived from the large megakaryocytes with increased DNA content.

scholarly journals Pengaruh Lama Pemberian Diet Tinggi Kolesterol terhadap Kadar LDL dan TGF-Β Serum Tikus Putih (Rattus novergicus) strain Wistar

2016 ◽  
Vol 5 (1) ◽  
Author(s):  
Biomechy Oktomalioputri ◽  
Eryati Darwin ◽  
Eva Decroli
Keyword(s):  
Growth Factor ◽  
Transforming Growth Factor ◽  
Low Density Lipoprotein ◽  
Density Lipoprotein ◽  
Transforming Growth Factor Β ◽  
Control Group ◽  
High Cholesterol Diet ◽  
Low Density ◽  
Cholesterol Diet ◽  
High Cholesterol

AbstrakDiet tinggi kolesterol ini akan meningkatkan kadar Low Density Lipoprotein (LDL) sebagai penanda hiperlipidemia yang berdampak pada terjadinya aterosklerosis. Transforming Growth Factor β (TGF-β) memiliki peranan dalam proses terjadinya aterosklerosis ini. Keterlibatannya dalam hiperlipidemia sebagai faktor risiko utama aterosklerosis belum banyak diketahui. Tujuan penelitian ini adalah menentukan pengaruh lama permberian diet tinggi kolesterol terhadap kadar LDL dan TGF-β pada tikus putih (Rattus novergicus) strain Wistar. Penelitian ini menggunakan metode post test only control group design yang dilakukan terhadap tikus Rattus novergicus jantan umur 3-4 bulan, berat 200-250 gram. Sampel penelitian terdiri dari 24 ekor tikus yang dibagi menjadi 4 kelompok, yaitu kelompok kontrol, A, B dan C. Selain kelompok kontrol, kelompok tikus diberi diet tinggi kolesterol berupa lemak kambing 10%, telur puyuh 5%, selama 10 hari untuk kelompok A, 20 hari untuk kelompok B dan 30 hari untuk kelompok C. Pada akhir percobaan darah tikus diambil dan dilakukan pemeriksaan kadar LDL dan TGF-β serum. Hasil penelitian diolah secara bivariat. Analisis yang digunakan yaitu uji oneway Anova. Hasil penelitian diketahui terdapat pengaruh lama pemberian diet tinggi kolesterol terhadap peningkatan kadar LDL serum tikus dengan p=0,01 (p<0,05). Terdapat pengaruh lama pemberian diet tinggi kolesterol terhadap penurunan kadar TGF-β dimana p=0,04 (p>0,05). Penelitian ini menyimpulkan bahwa terdapat pengaruh lama pemberian diet tinggi kolesterol terhadap kadar LDL dan tikus putih Rattus novergicus strain Wistar.Kata kunci: diet tinggi kolesterol, LDL, TGF-β AbstractHigh-cholesterol diet will increase Low Density Lipoprotein (LDL) levels which impact to atherosclerosis. Transforming Growth Factor β (TGF-β) play a role in atherosclerosis process. But its involvement in hyperlipidemia as the main risk factor of atherosclerosis still unknown. The objective of this study was to observe the effect of duration of giving  high-cholesterol diet on Low Density Lipoprotein and Transforming Growth Factor β levels white rats (Rattus novergicus) Wistar strain. This study uses  post-test only control group design, carried out on male rats Rattus novergicus 3-4 months, weight 200-250 grams. The study sample consisted of 24 rats were divided into 4 groups: control group, group A, group B and group C. Except  the control group, the group of rats given a high-cholesterol diet in the form of goat fat 10%, 5% quail eggs for 10 days to group A, group B for 20 days and 30 days for group C. At the end of the experiment blood was taken and examined LDL and TGF-β levels. Results were analyzed using bivariate. The analysis is oneway Anova test. The results of research known to have effect of duration on giving high-cholesterol diet to increase serum LDL levels of rats with p = 0.01 (p<0.05). This is the same case with TGF-β, which there was effect of duration on giving high-diet cholesterol diet to decrease serum TGF-β levels of rats where p = 0.04 (p>0.05). This study concludes that there are effect of duration on giving high-cholesterol diet to levels of LDL and TGF-β white rats Rattus novergicus Wistar strain.Keywords:  high-cholesterol diet, LDL, TGF β

Hypolipidemic and antioxidant effect of Ajuga iva in rats fed a high-cholesterol diet

Planta Medica ◽  
2006 ◽  
Vol 72 (11) ◽  
Author(s):  
MA Lacaille-Dubois ◽  
A Chenni ◽  
DA Yahia ◽  
FO Boukortt ◽  
J Prost ◽  
...  
Keyword(s):  
Antioxidant Effect ◽  
High Cholesterol Diet ◽  
Cholesterol Diet ◽  
High Cholesterol

Serum lipoproteins of diabetic rats fed a high cholesterol diet

Diabetes ◽  
1980 ◽  
Vol 29 (10) ◽  
pp. 774-777 ◽  
Author(s):  
C. M. Arbeeny ◽  
D. Edelstein ◽  
S. R. Freedman ◽  
H. A. Eder
Keyword(s):  
Diabetic Rats ◽  
High Cholesterol Diet ◽  
Cholesterol Diet ◽  
Serum Lipoproteins ◽  
High Cholesterol
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