Rapid and cost-effective fabrication of selectively permeable calcium-alginate microfluidic device using “modified” embedded template method

The development of a simple, portable, and cost-effective plasma separation platform for blood biochemical analysis is of great interest in clinical diagnostics. We represent a plasma separation microfluidic device using microspheres with different sizes as the separation barrier. This plasma separation device, with 18 capillary microchannels, can extract about 3 μL of plasma from a 50 μL blood sample in about 55 min. The effects of evaporation and the microsphere barrier on the plasma biochemical analysis results were studied. Correction factors were applied to compensate for these two effects. The feasibility of the device in plasma biochemical analysis was validated with clinical blood samples.

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Formulation and Mathematical Optimization of Controlled Release Calcium Alginate Micro Pellets of Frusemide

BioMed Research International ◽

10.1155/2013/819674 ◽

2013 ◽

Vol 2013 ◽

pp. 1-14 ◽

Cited By ~ 3

Author(s):

Amitava Ghosh ◽

Prithviraj Chakraborty

Keyword(s):

Sodium Alginate ◽

Calcium Alginate ◽

Mathematical Optimization ◽

Extended Period ◽

Cost Effective ◽

Polynomial Equation ◽

Chronic Hypertension ◽

Order Polynomial ◽

High Degree

Objective. Frusemide loaded calcium alginate micropellets, an oral microparticulate delivery system, was statistically optimized exhibiting prolonged therapeutic action minimizing its adverse effects.Methods. Ionotropic Gelation technique was adopted employing 32Factorial designs and keeping the entire process free from organic solvents. Physicochemical and the release characteristics of the prepared formulations were studied, keeping variations only in sodium alginate (primary polymer) and Acrycoat E30D (copolymer) dispersion.Result. Sodium alginate was predominant over Acrycoat E30D in all batches. Nonadditives or interaction was observed to be insignificant. Multiple regressions produced second-order polynomial equation, and the predictive results obtained were validated with high degree of correlation. Thein vivostudy applauded that optimized calcium alginate micropellets of frusemide can produce a much greater diuretic effect over an extended period of 24 hours.Conclusion. This study reveals that the potential of a single dose of the mathematically optimized micro pellets of frusemide formulation is sufficient in the management of peripheral edema and ascites in congestive heart failure and as well in the treatment of chronic hypertension, leading to better patient compliance, and can be produced with minimum experimentation and time, proving far more cost-effective formulation than the conventional methods of formulating dosage forms.

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Magnetic Control of an Electrochemical Microfluidic Device with an Arrayed Immunosensor for Simultaneous Multiple Immunoassays

Clinical Chemistry ◽

10.1373/clinchem.2006.085126 ◽

2007 ◽

Vol 53 (7) ◽

pp. 1323-1329 ◽

Cited By ~ 110

Author(s):

Dianping Tang ◽

Ruo Yuan ◽

Yaqin Chai

Keyword(s):

Tumor Markers ◽

Microfluidic Device ◽

Simultaneous Detection ◽

Reference Electrode ◽

Cost Effective ◽

Sio2 Nanoparticles ◽

Magnetic Bead ◽

Label Free ◽

Specific Tumor ◽

Protein Diagnostics

Abstract Background: Methods based on magnetic bead probes have been developed for immunoassay, but most involve complicated labeling or stripping procedures and are unsuitable for routine use. Methods: We synthesized magnet core/shell NiFe2O4/SiO2 nanoparticles and fabricated an electrochemical magnetic controlled microfluidic device for the detection of 4 tumor markers. The immunoassay system consisted of 5 working electrodes and an Ag/AgCl reference electrode integrated on a glass substrate. Each working electrode contained a different antibody immobilized on the NiFe2O4/SiO2 nanoparticle surface and was capable of measuring a specific tumor marker using noncompetitive electrochemical immunoassay. Results: Under optimal conditions, the multiplex immunoassay enabled the simultaneous detection of 4 tumor markers. The sensor detection limit was <0.5 μg/L (or <0.5 kunits/L) for most analytes. Intra- and interassay imprecisions (CVs) were <4.5% and 8.7% for analyte concentrations >5 μg/L (or >5 kunits/L), respectively. No nonspecific adsorption was observed during a series of procedures to detect target proteins, and electrochemical cross-talk (CV) between neighboring sites was <10%. Conclusion: This immunoassay system offers promise for label-free, rapid, simple, cost-effective analysis of biological samples. Importantly, the chip-based immunosensor could be suitable for use in the mass production of miniaturized lab-on-a-chip devices and open new opportunities for protein diagnostics and biosecurity.

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Carbon Nanofibers in Pure Form and in Calcium Alginate Composites Films: New Cost-Effective Antibacterial Biomaterials against the Life-Threatening Multidrug-Resistant Staphylococcus epidermidis

Polymers ◽

10.3390/polym11030453 ◽

2019 ◽

Vol 11 (3) ◽

pp. 453 ◽

Cited By ~ 14

Author(s):

Beatriz Salesa ◽

Miguel Martí ◽

Belén Frígols ◽

Ángel Serrano-Aroca

Keyword(s):

Carbon Nanofibers ◽

Staphylococcus Epidermidis ◽

Calcium Alginate ◽

Composite Films ◽

Cost Effective ◽

Multidrug Resistant ◽

Pure Form ◽

Production Costs ◽

World Health ◽

Human Beings

Due to the current global health problem of antibiotic resistant recently announced by the World Health Organization, there is an urgent necessity of looking for new alternative antibacterial materials able to treat and impede multidrug-resistant infections which are cost-effective and non-toxic for human beings. In this regard, carbon nanofibers (CNFs) possess currently much lower cost than other carbon nanomaterials, such as graphene oxide, and exhibit excellent chemical, mechanical and electric properties. Furthermore, here, the first report on the antibacterial activity of CNFs was demonstrated. Thus, these nanomaterials, in pure form or incorporated in a minuscule amount into calcium alginate composite films to reduce production costs as much as possible, showed to be new weapons against a globally spreading multidrug-resistant pathogen, the methicillin-resistant Staphylococcus epidermidis (MRSE). This Gram-positive bacterium is becoming one of the most dangerous pathogens, due to its abundance on skin. In this study, these hollow filamentous materials, in direct contact with cells and loaded in the low-cost calcium alginate composite films, showed no cytotoxicity for human keratinocyte HaCaT cells, which render them very promising for biomedical applications. The CNFs used in this work were characterized by Raman spectroscopy and observed by high-resolution transmission electron with energy-disperse X-ray spectroscopy.

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Shape-Controlled Production of Biodegradable Calcium Alginate Gel Microparticles Using a Novel Microfluidic Device

Langmuir ◽

10.1021/la061729+ ◽

2006 ◽

Vol 22 (22) ◽

pp. 9453-9457 ◽

Cited By ~ 151

Author(s):

Kan Liu ◽

Hui-Jiang Ding ◽

Jing Liu ◽

Yong Chen ◽

Xing-Zhong Zhao

Keyword(s):

Microfluidic Device ◽

Calcium Alginate ◽

Alginate Gel ◽

Calcium Alginate Gel ◽

Shape Controlled

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Citizen-Led Sampling to Monitor Phosphate Levels in Freshwater Environments Using a Simple Paper Microfluidic Device

10.26434/chemrxiv.14626920.v1 ◽

2021 ◽

Author(s):

Samantha Richardson ◽

Alexander Iles ◽

Jeanette M. Rotchell ◽

Tim Charlson ◽

Annabel Hanson ◽

...

Keyword(s):

Microfluidic Device ◽

Mobile Technology ◽

High Frequency ◽

Microfluidic Devices ◽

Cost Effective ◽

Smartphone App ◽

Test Device ◽

Monitoring Campaign ◽

Paper Microfluidic ◽

Freshwater Environments

We demonstrate how a combination of paper microfluidic devices and handheld mobile technology can be used by citizen scientists to carry out a sustained water monitoring campaign. We have developed a paper-based analysis device and a 3 minute sampling workflow that requires no more than a container, a test device and a smartphone app. The contaminant measured in these pilots are phosphates, detectable down to 3 mg L<sup>-1</sup>. Together these allow volunteers to successfully carry out cost-effective, high frequency, phosphate monitoring over an extended geographies and periods.

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A Textile-Based Microfluidic Platform for the Detection of Cytostatic Drug Concentration in Sweat Samples

Applied Sciences ◽

10.3390/app10124392 ◽

2020 ◽

Vol 10 (12) ◽

pp. 4392 ◽

Cited By ~ 1

Author(s):

Goran M. Stojanović ◽

Maja M. Radetić ◽

Zoran V. Šaponjić ◽

Marija B. Radoičić ◽

Milan R. Radovanović ◽

...

Keyword(s):

Microfluidic Device ◽

Cost Effective ◽

Electrical Contacts ◽

Cytostatic Drug ◽

Variable Resistor ◽

Microfluidic Platform ◽

Textile Fabrics ◽

Drug Concentrations ◽

Artificial Sweat ◽

Conductive Textile

This work presents a new multilayered microfluidic platform, manufactured using a rapid and cost-effective xurography technique, for the detection of drug concentrations in sweat. Textile fabrics made of cotton and polyester were used as a component of the platform, and they were positioned in the middle of the microfluidic device. In order to obtain a highly conductive textile, the fabrics were in situ coated with different amounts of polyaniline and titanium dioxide nanocomposite. This portable microfluidic platform comprises at least three layers of optically transparent and flexible PVC foils which were stacked one on top of the other. Electrical contacts were provided from the edge of the textile material when a microfluidic variable resistor was actually created. The platform was tested in plain artificial sweat and in artificial sweat with a dissolved cytostatic test drug, cyclophosphamide, of different concentrations. The proposed microfluidic device decreased in resistance when the sweat was applied. In addition, it could successfully detect different concentrations of cytostatic medication in the sweat, which could make it a very useful tool for simple, reliable, and fast diagnostics.

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Cost-effective fabrication of photopolymer molds with multi-level microstructures for PDMS microfluidic device manufacture

RSC Advances ◽

10.1039/c9ra07955f ◽

2020 ◽

Vol 10 (7) ◽

pp. 4071-4079 ◽

Cited By ~ 2

Author(s):

Carol M. Olmos ◽

Ana Peñaherrera ◽

Gustavo Rosero ◽

Karla Vizuete ◽

Darío Ruarte ◽

...

Keyword(s):

Microfluidic Device ◽

Cost Effective ◽

Mold Fabrication ◽

Multi Level

This paper describes a methodology of photopolymer mold fabrication with multi-level microstructures for polydimethylsiloxane (PDMS) microfluidic device manufacture.

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