scholarly journals Mineral metabolism of pregnant goats under feed restriction

2017 ◽  
Vol 57 (2) ◽  
pp. 290 ◽  
Author(s):  
Carla J. Härter ◽  
Lisiane D. Lima ◽  
Douglas S. Castagnino ◽  
Astrid R. Rivera ◽  
Alana M. Nunes ◽  
...  

We examined the effects of feed restriction on calcium, phosphorus, magnesium, sodium and potassium metabolism in Oberhasli and Saanen goats during gestation. The 63 goats were distributed into groups that were divided into three levels of feed restriction (0%, 20% and 40% restriction) and slaughtered at different pregnancy stages (80, 110 and 140 days of gestation), in a randomised block design with a 2 × 3 × 3 factorial arrangement. The mineral balance was determined at ~80, 110 and 140 days of gestation. The serum levels of minerals and alkaline phosphatase activity were determined during pregnancy. Mineral retention in the maternal body, femur, empty uterus, mammary gland, fetus and fetal fluid was also determined during gestation. Bone mineral density was measured in the femur. Mixed models with days of gestation, levels of feed restriction, breed and their interactions as fixed effects and blocks as random effect were used for data analysis. In response to the reduction in feed intake, the maternal body uses its mineral reserves to maintain gestation. Physiological adjustments of the goats subjected to 20% feed restriction avoided a decrease in fetal mineral deposition. More severe feed restriction, however, compromised concentrations of phosphorus, sodium and potassium in the fetus, which were the main minerals used by the maternal body, whereas calcium and magnesium deposition in fetuses remained unaffected. At 40% feed restriction, the retention of all minerals in the body decreased, and the fetal dry mass was on average also less than those fetuses from goats without feed restriction. The fetal deposition of phosphorus, sodium and potassium was also lower during 40% restriction.

2019 ◽  
Vol 59 (12) ◽  
pp. 2252
Author(s):  
Paula Fernanda Varella Santos ◽  
Carla Joice Härter ◽  
Nhayandra Christina Dias e Silva ◽  
Rafael Fernandes Leite ◽  
Fernanda Oliveira de Miranda Figueiredo ◽  
...  

The objective of the present study was to evaluate the effects of feed restriction on calcium (Ca), phosphorus (P) and magnesium metabolism in goat kids from 15 to 30 kg bodyweight, and to evaluate the role of sex in these processes. The study used a split plot design comprising three sex groups (intact males, castrated males and females), and the subplot comprised three levels of feed restriction (0% (ad libitum), 25% and 50%). Mineral intake and retention, mineral concentration in the blood, alkaline phosphatase activity and bone mineral density (BMD) of the femur were determined. The data were analysed as mixed models. Daily Ca, P and magnesium retention in the body decreased linearly with increasing feed restriction (P < 0.05). At 50% feed restriction, we observed a 22% reduction of alkaline phosphatase activity, and 9% and 7% reductions of Ca and P contents in blood serum. The BMD of females fed ad libitum was greater than castrated and intact males, whereas when subjected to 50% feed restriction, no differences in BMD were noted among the sexes (P < 0.01). Irrespective of feed restriction, females tended to retain less P in their bodies (P < 0.10) and tended to have the lowest P serum concentrations (P = 0.08). Our results indicated that only females showed decreased BMD under feed restriction; Ca, P and magnesium metabolism in prepubertal Saanen goat kids was mainly affected by feed restriction, whereas sex mainly affected the P metabolism.


2020 ◽  
Vol 98 (Supplement_3) ◽  
pp. 137-138
Author(s):  
Calvin Gibbons ◽  
Andrea K Watson ◽  
Galen E Erickson ◽  
Bradley M Boyd ◽  
Levi J McPhillips ◽  
...  

Abstract Algae oil production for Omega-3 fatty acid supplementation yields a byproduct called Condensed Algal Residue Solubles (CARS; 25.4% DM, 19.3% CP, 8.3% Fat, 9.96% Na on DM basis), de-oiled algae cells with residual fermentation substrates. This study evaluated the use of CARS in feedlot finishing diets. Crossbreed steers, (n=480) were blocked and stratified by initial body weight (BW) into 4 blocks, and assigned randomly to treatments. Treatments were designed as a 2 x 3 factorial with 3 inclusions of CARS (0, 2.5, 5% of diet DM) and 2 different base diets representing Northern and Southern Great Plains diets. The Southern diets contained steam flaked corn and dry distillers grains while the Northern diets had dry rolled and high moisture corn with wet distillers grains. CARS replaced corn in both diets. All blocks were harvested after 148 days on feed. Performance data were analyzed as a randomized block design with CARS inclusion, base diet, and interactions as fixed effects, BW block as a random effect and pen (n=48) as the experimental unit. Orthogonal contrasts were used to test linear and quadratic effects of CARS inclusion. There were no significant interactions between CARS inclusion and diet type (P ≥ 0.49). Main effects of CARS indicated positive quadratic responses for carcass adjusted ADG, G:F, 12th rib back fat, yield grade (P < 0.01; increasing to 2.5% inclusion, decreasing at 5%) and hot carcass weight was both linear and quadratic (P ≤ 0.01 and P ≥ 0.06 respectively; 969, 977, 935 as CARS increased). Linear decrease in DMI, final adjusted BW and ribeye area (P ≤ 0.01) as CARS increased. Cattle fed the Southern diets had greater ADG and G:F compared to Northern diets (P < 0.01). Including 2.5% CARS in the diet improved feed efficiency in both Northern and Southern based feedlot diets.


2021 ◽  
Vol 99 (Supplement_3) ◽  
pp. 166-167
Author(s):  
Andrea M Osorio ◽  
Kaue T Tonelli Nardi ◽  
Igor Gomes Fávero ◽  
Kaliu G Scaranto Silva ◽  
Kymberly D Coello ◽  
...  

Abstract The effects of a nutritional packet were evaluated on CH4 emissions and apparent total tract nutrient digestibility of feedlot beef steers. Thirty Angus-crossbred steers (BW = 542 ± 8.4 kg) were used in a randomized complete block design and allocated into pens equipped with SmartFeed (C-Lock; 15 steers/treatment). Steers were consuming a steam-flaked corn-based diet (88% concentrate DM basis) ad libitum for the last 65 d on feed, and received the following treatments: 1) control and 2) a nutritional packet [0.29% DM basis; live yeast (Saccharomyces cerevisiae; 8.7 Log CFU/g); Vitamin C (5.4 g/kg); Vitamin B1 (13.33 g/kg); NaCl (80 g/kg); KCl (80 g/kg)]. Methane emissions and apparent total tract nutrient digestibility were measured during 3 periods with 5-d of collections each. Gas emissions from steers were measured utilizing the SF6 tracer technique. Feed and fecal samples were collected once and twice (0700 h and 1600 h) daily, respectively, to determine digestibility of nutrients using iNDF as an internal marker. Steer was considered the experimental unit. Data were analyzed as repeated measures using the MIXED procedure of SAS with the fixed effects of treatment, period, and their interaction, and the random effect of block. No treatment × period interactions (P ≥ 0.125) were observed for DMI and any of the CH4 production variables (g/day, g/kg BW0.75, g/nutrient intake, and g/nutrient digested). Moreover, treatments did not affect digestibility of DM, OM, or ADF (P ≥ 0.300); however, digestibility of NDF was increased for treated cattle (P = 0.013), which resulted in a tendency (P = 0.098) to decrease CH4 production in g per kg NDF intake and decreased (P = 0.020) grams CH4 per kg NDF digested. The nutritional packet may be altering ruminal fermentation on intensively managed steers and improving fiber digestibility, which can have benefits on CH4 emission intensity.


2019 ◽  
Vol 97 (Supplement_3) ◽  
pp. 165-165
Author(s):  
Nadira J Espinoza-Rock ◽  
Andrea O Doblado ◽  
Sebastian E Mejia-Turcios ◽  
Evandro Dias ◽  
Michael Sandes ◽  
...  

Abstract A randomized complete block design was used to determine the effects of 4 concentrations of 4 essential oils (EO) on in vitro ruminal fermentation variables. In vitro fermentation consisted of 0.7 g of high concentrate substrate (86.7% DM) and 50 mL of 2:1 buffer:ruminal fluid inoculum incubated for 24 h for each batch (n = 3; separate days) Treatments were arranged as a 4 × 5 factorial. Factors included 4 EO (eugenol, cinnamic aldehyde, anethole, and garlic oil) at 5 concentrations (0, 10, 75, 200, and 400 mg/L of inoculum). Data were analyzed using the MIXED procedure of SAS with the fixed effects of EO, concentration, and their interaction, and random effect of day (block). Batch was considered the experimental unit. There was an interaction (P < 0.001) for total gas production, where a cubic effect (P ≤ 0.041) was observed for eugenol, cinnamic aldehyde, and anethole, and a quadratic effect (P = 0.001) was observed for garlic oil. No interactions (P > 0.05) were observed for in vitro OM digestibility (IVOMD) or CH4 production. There was an effect of EO (P < 0.001) on IVOMD, where eugenol reduced (P ≤ 0.007) digestibility compared with anethole and garlic oil, which promoted the greatest (P ≤ 0.029) IVOMD. Methane production (mmol/g OM fermented) was affected by EO (P < 0.001), where it was decreased (P ≤ 0.001) by garlic oil compared with all other EO. There was an interaction (P < 0.001) for H2S production (µmol/g OM fermented), where it was linearly decreased (P = 0.003) and linearly increased (P < 0.001) as concentrations of eugenol and garlic oil increased, respectively. These EO had contradictory impacts on in vitro ruminal fermentation, thus combining them could potentially improve multiple aspects of in vitro and in vivo fermentation.


2021 ◽  
Vol 99 (Supplement_3) ◽  
pp. 166-166
Author(s):  
Kenneth S Madrid ◽  
Andrea M Osorio ◽  
Francine M Ciriaco ◽  
Kymberly D Coello ◽  
Angel A Raudales ◽  
...  

Abstract A randomized complete block design was used to evaluate the effects of bismuth subsalicylate (BSS) on in vitro ruminal fermentation with differing concentrations of sulfate. In vitro fermentation consisted of 50 mL of a 4:1 buffer:ruminal fluid inoculum and 0.7 g (pre-dehydrated) of substrate [WW-B Dahl bluestem hay (Bothriochloa bladhii)] incubated for 48 h (39oC). Treatments were arranged as a 3 × 4 factorial with concentration of sulfate (0.2, 2.9, or 5.6 g sulfate/L buffer) and BSS (0.0, 0.165, 0.330, or 0.495% substrate DM) as the main factors. In vitro organic matter digestibility (IVOMD), and CH4, H2S, and total gas production (TGP) were measured. Data were analyzed using the MIXED procedure of SAS with the fixed effects of BSS, sulfate, and their interaction. Incubation day (block) was considered a random effect. The average of 2 bottles within day was considered experimental unit. A BSS × sulfate interaction was observed for TGP (P = 0.040) and H2S production (P < 0.001), where BSS had a larger negative impact on TGP and production of H2S with greater concentrations of sulfate. A linear effect (P < 0.001) of sulfate was observed for CH4 production per gram of incubated OM, where CH4 was decreased as sulfate concentration increased. A quadratic effect of sulfate was observed for IVOMD (P = 0.010) and pH (P = 0.009). Production of H2S linearly decreased (P = 0.001) as BSS concentration increased. The addition of BSS to in vitro incubations did not affect (P > 0.10) any other variables measured. Bismuth subsalicylate does not appear to have negative effects on in vitro fermentation parameters while decreasing H2S production; however, elevated concentrations of sulfate in the buffer appears to have negative impacts on fermentation. Further in vivo research is warranted to support BSS supplementation to cattle with high dietary sulfate.


Author(s):  
Sebastian E Mejia-Turcios ◽  
Andrea M Osorio ◽  
Francine M Ciriaco ◽  
Phil M Urso ◽  
Rafael C Araujo ◽  
...  

Abstract Two experiments were performed to evaluate the effects of bismuth subsalicylate (BSS) and calcium-ammonium nitrate (CAN) on in vitro ruminal fermentation, growth, apparent total tract digestibility of nutrients, liver mineral concentration, and carcass quality of beef cattle. In Exp. 1, 4 ruminally cannulated steers [520 ± 30 kg body weight (BW)] were used as donors to perform a batch culture and an in vitro organic matter digestibility (IVOMD) procedure. Treatments were arranged in a 2 × 2 factorial with factors being BSS [0 or 0.33% of substrate dry matter (DM)] and CAN (0 or 2.22% of substrate DM). In Exp. 2, 200 Angus-crossbred steers (385 ± 27 kg BW) were blocked by BW and allocated to 50 pens (4 steers/pen) in a randomized complete block design with a 2 × 2 + 1 factorial arrangement of treatments. Factors included BSS (0 or 0.33% of the diet DM) and non-protein nitrogen (NPN) source [urea or encapsulated CAN (eCAN) included at 0.68 or 2.0% of the diet, respectively] with 0.28% ruminally available S (RAS). A low S diet was included as a positive control containing urea (0.68% of DM) and 0.14% RAS. For Exp. 1, data were analyzed using the MIXED procedure of SAS with the fixed effects of BSS, CAN, BSS × CAN, and the random effect of donor. For Exp. 2, the MIXED procedure of SAS was used for continuous variables and the GLIMMIX procedure for categorical data. For Exp. 1, no differences (P > 0.230) were observed for IVOMD. There was a tendency (P = 0.055) for an interaction regarding H2S production. Acetate:propionate increased (P = 0.003) with the addition of CAN. In Exp. 2, there was a NPN source effect (P = 0.032) where steers consuming urea had greater carcass-adjusted final shrunk BW than those consuming eCAN. Intake of DM (P < 0.001) and carcass-adjusted average daily gain (P = 0.024) were reduced by eCAN; however, it did not affect (P = 0.650) carcass-adjusted feed efficiency. Steers consuming urea had greater (P = 0.032) hot carcass weight, and a BSS × NPN interaction (P = 0.019) was observed on calculated yield grade. Apparent absorption of S decreased (P < 0.001) with the addition of BSS. Final liver Cu concentration was reduced (P = 0.042) by 58% in cattle fed BSS, indicating that BSS may decrease Cu absorption and storage in the liver. The results observed in this experiment indicate that BSS does not have negative effects on feedlot steer performance whereas CAN may hinder performance of steers fed finishing diets.


2019 ◽  
Vol 97 (Supplement_3) ◽  
pp. 157-157
Author(s):  
Koryn S Hare ◽  
Greg B Penner ◽  
Katie M Wood

Abstract The objective of this study was to determine whether antepartum glucose-insulin kinetics were affected when primiparous Hereford-cross heifers were fed diets containing 100 (CON; n = 10) or 133% (HMP; n = 11) of their metabolizable protein requirements from d -55.3 ± 3.7 until parturition. Intravenous glucose tolerance tests (IVGTT) were performed on d -5.4 and -7.1 ± 1.3 (CON and HMP, respectively; P = 0.34) by collecting baseline (-10 min) plasma, infusing 1.36 g glucose/kg BW0.75 via a jugular catheter, and subsequently sampling plasma for analysis of glucose and insulin. Data were analyzed with PROC GLIMMIX as a randomized block design with the fixed effects of diet, time (minute), and diet × time, considering cow (block) as a random effect. Pearson correlations were performed for IVGTT variables and test-day relative to parturition. Differences and trends were declared at P < 0.05 and 0.05 ≤ P < 0.10, respectively. Basal glucose was 13.5% greater (P = 0.048) and basal insulin tended to be 28.8% greater (P = 0.068) for HMP than CON heifers. Plasma glucose (-10 to 120 min) was greater (P = 0.047) for HMP than CON, whereas insulin was not (P = 0.54). Glucose and insulin were respectively elevated (time: P < 0.001) at 0 and 5 and 5 to 20 min and both equilibrated to baseline by 90- and 120-min. Diet × time tended to affect (P = 0.061) the glucose:insulin ratio. Test-day was correlated with time-to-max insulin (ρ = 0.60, P = 0.006), insulin clearance rate (ρ = 0.48, P = 0.027), and glucose area-under-the-curve (ρ = -0.47, P = 0.031). Time relative to calving can influence antepartum glucose-insulin kinetics. Oversupplying metabolizable protein increased baseline glucose and insulin and glucose throughout the IVGTT. Antepartum beef cattle may have altered energy partitioning that is responsive to protein consumption.


2019 ◽  
Vol 97 (Supplement_2) ◽  
pp. 34-34
Author(s):  
Belcy Karine Angarita Barajas ◽  
Rodolfo Cantet ◽  
Kaitlin E Wurtz ◽  
Carly O’Malley ◽  
Janice Siegford ◽  
...  

Abstract Traditional social genetic effects modeling assumes uniform intensity of interaction between group members. Tree breeders proposed relaxing this assumption by incorporating estimates of intensity of competition between pairs of individuals. Here, we incorporated the quantification of aggressive interactions between pairs of animals in the estimation of indirect social genetic effects on skin lesions in the anterior part of the body in growing pigs. The data consisted of 491 pigs (215 barrows and 276 gilts, mean of 66 ±5 days of age). Animals were housed in 37 pens (11 to 15 pigs by pen) over 7 replicates. Trained scorers counted the number of skin lesions immediately before and 24 hours after mixing pigs. Animals were video-recorded for 9 hours post mixing and trained observers quantified the type and duration of aggressive interactions between pairs of pigs. The number of skin lesions in the frontal part of the body 24 hours post-mixing was the response variable and the number of seconds that pairs of animals spent engaged in reciprocal fights was used to quantify the intensity of interaction. We compared three different models: A direct genetic additive model (DGE), a traditional social genetic effect model (TSGE) assuming uniform interactions, and an improved social genetic effect model (ISGE) that used intensity of interaction to parameterize social genetic effects. All models included fixed effects of sex, replicate, lesion scorer, initial weight and pre-mixing lesion count; a random effect of pen; and a random direct genetic effect. The model ISGE recovered the most variance (smallest σe2) and resulted in the highest estimated h2 (P < 0.005). The model TSGE produced estimates that did not differ significantly from DGE (P = 1). Contrarily, incorporating the intensity of interaction into the modeling of ISGE allowed direct and indirect genetic effects to be estimated separately, even in a small dataset.


2019 ◽  
Vol 97 (10) ◽  
pp. 4334-4340
Author(s):  
Tessa M Schulmeister ◽  
Martin Ruiz-Moreno ◽  
Gleise M Silva ◽  
M Garcia-Ascolani ◽  
Francine M Ciriaco ◽  
...  

Abstract Brassica carinata is a new oilseed crop in Florida with the potential of producing high-quality jet biofuel. A high-protein meal (~40% crude protein; CP) is obtained as a byproduct of oil extraction; however, limited research is available on the utilization of this meal as a protein supplement for beef cattle. A generalized randomized block design was used to evaluate the effects of supplementation with B. carinata meal pellets on performance and attainment of puberty in growing beef heifers consuming bermudagrass hay (Cynodon dactylon) ad libitum. Sixty-four Angus crossbred heifers (240 ± 39 kg initial body weight; BW) were stratified and blocked (2 blocks: light and heavy) by initial BW and randomly allocated into 18 pens over 2 consecutive years (10 in year 1 and 8 in year 2). Within block, pens were randomly assigned to 1 of 2 treatments: 0 (CTL) or 0.3% of BW/d (as fed) of B. carinata meal pellets (BCM). Blood samples and BW were collected weekly for 70 d, before daily supplementation. Data were analyzed using PROC MIXED of SAS with repeated measures. Model included the fixed effects of treatment, day, treatment × day interactions, block, and block × treatment interactions, with the random effect of year. Plasma was analyzed for concentrations of progesterone, triiodothyronine (T3), thyroxine (T4), ceruloplasmin (Cp), and haptoglobin (Hp). An effect of treatment was observed (P ˂ 0.01) for ADG between CTL (0.14 kg) and BCM (0.42 kg). There was no treatment or block (P > 0.05) effect for concentrations of T3, T4, or Hp; however, there was an effect of day (P < 0.01) for T3, T4, and Cp. An effect of treatment (P ˂ 0.01) was observed for Cp, with CTL having greater concentrations compared with BCM. Time to attainment of puberty did not differ (P = 0.93) between treatments. Feeding B. carinata meal as a protein supplement at 0.3% of BW/d is a viable option for increasing ADG of growing beef heifers, without affecting attainment of puberty, thyroid hormone status, or eliciting an acute phase response.


2021 ◽  
Vol 99 (Supplement_1) ◽  
pp. 127-128
Author(s):  
Taylor M Smock ◽  
Dale Woerner ◽  
Kristin E Hales

Abstract One hundred ninety-two beef steers (BW = 332 ± 8.2 kg) were used in a randomized complete block design to evaluate the effects of differing bunk management and bulk density of steam-flaked corn (SFC) in a randomized complete block design. A factorial arrangement of treatments was used with: 1) slick bunk management (SBM) + 335 g/L SFC; 2) modified ad libitum bunk management (MAL) + 335 g/L SFC; 3) SBM + 425 g/L SFC; 4) MAL+ 425 g/L SFC. Steers were randomly assigned to treatment within BW block, with 12 pen replications per treatment. Data were analyzed using a mixed model with fixed effects of bunk management, SFC bulk density, and their interaction. Block was included as a random effect. Bunk management strategy did not affect growth performance, carcass characteristics, or liver abscess score (P &gt; 0.10). The average daily gain (ADG) of steers fed 425 g/L SFC was greater (P = 0.05) from days 35 to 105 than those fed 335 g/L SFC; however, overall ADG did not differ (P = 0.36). The DMI of steers fed 425 g/L SFC was greater (P ≤ 0.05) than those fed 335 g/L SFC. Gain-to-feed of steers fed 425 g/L SFC tended (P = 0.10) to be lesser from days 0 to 35 but did not differ overall (P ≥ 0.12). Steers fed 425 g/L SFC tended to have greater backfat and calculated empty body fat (P ≤ 0.07) than those fed 335 g/L SFC and had a greater calculated yield grade (P = 0.05). Steers fed 425 g/L SFC had 43.51% fewer (P = 0.04) liver abscesses. Bunk management strategy did not impact growth performance or carcass characteristics; whereas, SFC processed to 425 g/L increased DMI, fat thickness, and yield grade while resulting in a decreased proportion of liver abscesses.


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