scholarly journals 393 Beef cow antepartum glucose-insulin kinetics are altered by overfeeding metabolizable protein during late gestation and influenced by test-day relative to calving

2019 ◽  
Vol 97 (Supplement_3) ◽  
pp. 157-157
Author(s):  
Koryn S Hare ◽  
Greg B Penner ◽  
Katie M Wood
Keyword(s):  
Fixed Effects ◽  
Block Design ◽  
Random Effect ◽  
Area Under The Curve ◽  
Late Gestation ◽  
Ratio Test ◽  
Intravenous Glucose ◽  
Metabolizable Protein ◽  
Insulin Kinetics ◽  
Randomized Block Design

Abstract The objective of this study was to determine whether antepartum glucose-insulin kinetics were affected when primiparous Hereford-cross heifers were fed diets containing 100 (CON; n = 10) or 133% (HMP; n = 11) of their metabolizable protein requirements from d -55.3 ± 3.7 until parturition. Intravenous glucose tolerance tests (IVGTT) were performed on d -5.4 and -7.1 ± 1.3 (CON and HMP, respectively; P = 0.34) by collecting baseline (-10 min) plasma, infusing 1.36 g glucose/kg BW0.75 via a jugular catheter, and subsequently sampling plasma for analysis of glucose and insulin. Data were analyzed with PROC GLIMMIX as a randomized block design with the fixed effects of diet, time (minute), and diet × time, considering cow (block) as a random effect. Pearson correlations were performed for IVGTT variables and test-day relative to parturition. Differences and trends were declared at P < 0.05 and 0.05 ≤ P < 0.10, respectively. Basal glucose was 13.5% greater (P = 0.048) and basal insulin tended to be 28.8% greater (P = 0.068) for HMP than CON heifers. Plasma glucose (-10 to 120 min) was greater (P = 0.047) for HMP than CON, whereas insulin was not (P = 0.54). Glucose and insulin were respectively elevated (time: P < 0.001) at 0 and 5 and 5 to 20 min and both equilibrated to baseline by 90- and 120-min. Diet × time tended to affect (P = 0.061) the glucose:insulin ratio. Test-day was correlated with time-to-max insulin (ρ = 0.60, P = 0.006), insulin clearance rate (ρ = 0.48, P = 0.027), and glucose area-under-the-curve (ρ = -0.47, P = 0.031). Time relative to calving can influence antepartum glucose-insulin kinetics. Oversupplying metabolizable protein increased baseline glucose and insulin and glucose throughout the IVGTT. Antepartum beef cattle may have altered energy partitioning that is responsive to protein consumption.

scholarly journals 251 Evaluation of Condensed Algal Residue Solubles as an Ingredient in Cattle Finishing Diets

2020 ◽  
Vol 98 (Supplement_3) ◽  
pp. 137-138
Author(s):  
Calvin Gibbons ◽  
Andrea K Watson ◽  
Galen E Erickson ◽  
Bradley M Boyd ◽  
Levi J McPhillips ◽  
...  
Keyword(s):  
Great Plains ◽  
Fixed Effects ◽  
Block Design ◽  
Random Effect ◽  
Distillers Grains ◽  
Experimental Unit ◽  
Omega 3 ◽  
Finishing Diets ◽  
Randomized Block Design ◽  
Algae Oil

Abstract Algae oil production for Omega-3 fatty acid supplementation yields a byproduct called Condensed Algal Residue Solubles (CARS; 25.4% DM, 19.3% CP, 8.3% Fat, 9.96% Na on DM basis), de-oiled algae cells with residual fermentation substrates. This study evaluated the use of CARS in feedlot finishing diets. Crossbreed steers, (n=480) were blocked and stratified by initial body weight (BW) into 4 blocks, and assigned randomly to treatments. Treatments were designed as a 2 x 3 factorial with 3 inclusions of CARS (0, 2.5, 5% of diet DM) and 2 different base diets representing Northern and Southern Great Plains diets. The Southern diets contained steam flaked corn and dry distillers grains while the Northern diets had dry rolled and high moisture corn with wet distillers grains. CARS replaced corn in both diets. All blocks were harvested after 148 days on feed. Performance data were analyzed as a randomized block design with CARS inclusion, base diet, and interactions as fixed effects, BW block as a random effect and pen (n=48) as the experimental unit. Orthogonal contrasts were used to test linear and quadratic effects of CARS inclusion. There were no significant interactions between CARS inclusion and diet type (P ≥ 0.49). Main effects of CARS indicated positive quadratic responses for carcass adjusted ADG, G:F, 12th rib back fat, yield grade (P < 0.01; increasing to 2.5% inclusion, decreasing at 5%) and hot carcass weight was both linear and quadratic (P ≤ 0.01 and P ≥ 0.06 respectively; 969, 977, 935 as CARS increased). Linear decrease in DMI, final adjusted BW and ribeye area (P ≤ 0.01) as CARS increased. Cattle fed the Southern diets had greater ADG and G:F compared to Northern diets (P < 0.01). Including 2.5% CARS in the diet improved feed efficiency in both Northern and Southern based feedlot diets.

528 Late-Breaking: Late Gestation Metabolizable Energy Intake Increases Colostrum Yield and Alters Colostrum Composition in Beef Cattle

2021 ◽  
Vol 99 (Supplement_3) ◽  
pp. 150-150
Author(s):  
Koryn S Hare ◽  
Emily Croft ◽  
Katharine M Wood ◽  
Michael A Steele
Keyword(s):  
Fixed Effects ◽  
Block Design ◽  
Compositional Analysis ◽  
Repeated Measurements ◽  
Metabolizable Energy ◽  
Late Gestation ◽  
Beef Cow ◽  
Randomized Block Design ◽  
Milking Machine ◽  
Beta Hydroxybutyrate

Abstract The objective was to determine how late gestation metabolizable energy (ME) intake impacts beef cow colostrogenesis. Angus-Simmental primiparous (n = 47) and multiparous (n = 109) cattle were blocked by expected calving date and randomly assigned to receive diets that supplied 80% (LME; n = 53), 100% (CME; n = 52), or 120% (HME; n = 51) of the ME requirement. Treatments were balanced by initial BW and fed for 53 d prior to calving. Cow body weight was recorded d -53, -39, -25, -10, and -3 relative to calving and retrospectively conceptus-corrected. Calves were weighed and measured prior to suckling. Colostrum was hand-stripped from all cows for compositional analysis and a subset (n = 16/treatment) was fully milked using a milking machine. Data were analyzed (PROC GLIMMIX) as a randomized block design, including the fixed effects of treatment, parity, and treatment×parity and the random effects of block and cow(block). Day and its interactions were included for repeated measurements. Treatment did not affect (P ≥ 0.73) cow BW or conceptus-corrected BW. Calf chest circumference was lesser (treatment×parity: P = 0.023) for primiparous vs. multiparous calves if their dams consumed LME or CME, but not if their dams consumed HME. Feeding HME rather than LME increased (P = 0.006) colostrum yield. The LME diet increased (P = 0.004) colostrum IgG concentration over HME; however, HME colostrum yielded more (P = 0.005) total IgG. Crude protein and urea-N were elevated (P < 0.001) in LME colostrum, whereas HME colostrum contained more (P = 0.026) lactose. Low ME colostrum fat was greater (P = 0.013) than CME and beta-hydroxybutyrate was increased (P < 0.001) in LME vs. CME and HME colostrum. These data provide insight regarding nutrient partitioning during late gestation and demonstrate that beef cow colostrogenesis is responsive to prepartum ME intake.

305 Methane Emissions and Apparent Total Tract Nutrient Digestibility of Feedlot Beef Steers Under Intensive Management with or Without an Added Nutritional Packet

2021 ◽  
Vol 99 (Supplement_3) ◽  
pp. 166-167
Author(s):  
Andrea M Osorio ◽  
Kaue T Tonelli Nardi ◽  
Igor Gomes Fávero ◽  
Kaliu G Scaranto Silva ◽  
Kymberly D Coello ◽  
...  
Keyword(s):  
Treatment Period ◽  
Repeated Measures ◽  
Fixed Effects ◽  
Block Design ◽  
Random Effect ◽  
Methane Emissions ◽  
Nutrient Digestibility ◽  
Experimental Unit ◽  
Beef Steers ◽  
Ch4 Production

Abstract The effects of a nutritional packet were evaluated on CH4 emissions and apparent total tract nutrient digestibility of feedlot beef steers. Thirty Angus-crossbred steers (BW = 542 ± 8.4 kg) were used in a randomized complete block design and allocated into pens equipped with SmartFeed (C-Lock; 15 steers/treatment). Steers were consuming a steam-flaked corn-based diet (88% concentrate DM basis) ad libitum for the last 65 d on feed, and received the following treatments: 1) control and 2) a nutritional packet [0.29% DM basis; live yeast (Saccharomyces cerevisiae; 8.7 Log CFU/g); Vitamin C (5.4 g/kg); Vitamin B1 (13.33 g/kg); NaCl (80 g/kg); KCl (80 g/kg)]. Methane emissions and apparent total tract nutrient digestibility were measured during 3 periods with 5-d of collections each. Gas emissions from steers were measured utilizing the SF6 tracer technique. Feed and fecal samples were collected once and twice (0700 h and 1600 h) daily, respectively, to determine digestibility of nutrients using iNDF as an internal marker. Steer was considered the experimental unit. Data were analyzed as repeated measures using the MIXED procedure of SAS with the fixed effects of treatment, period, and their interaction, and the random effect of block. No treatment × period interactions (P ≥ 0.125) were observed for DMI and any of the CH4 production variables (g/day, g/kg BW0.75, g/nutrient intake, and g/nutrient digested). Moreover, treatments did not affect digestibility of DM, OM, or ADF (P ≥ 0.300); however, digestibility of NDF was increased for treated cattle (P = 0.013), which resulted in a tendency (P = 0.098) to decrease CH4 production in g per kg NDF intake and decreased (P = 0.020) grams CH4 per kg NDF digested. The nutritional packet may be altering ruminal fermentation on intensively managed steers and improving fiber digestibility, which can have benefits on CH4 emission intensity.

scholarly journals Estimation of direct and maternal genetic parameters for weaning weight in Hungarian Simmental cattle

2021 ◽  
pp. 17-22
Author(s):  
Afees Abiola Ajasa ◽  
Imre Füller ◽  
Barnabás Vágó ◽  
István Komlósi ◽  
János Posta
Keyword(s):  
Fixed Effects ◽  
Genetic Parameters ◽  
Additive Genetic Variance ◽  
Random Effect ◽  
Ratio Test ◽  
Genetic Covariance ◽  
Weaning Weight ◽  
Simmental Cattle ◽  
Estimate Variance ◽  
Best Fit

The aim of the current research was to estimate variance components and genetic parameters of weaning weight in Hungarian Simmental cattle. Weaning weight records were obtained from the Association of Hungarian Simmental Breeders. The dataset comprised of 44,278 animals born from 1975 to 2020. The data was analyzed using the restricted maximum likelihood methodology of the Wombat software. We fitted a total of six models to the weaning weight data of Hungarian Simmental cattle. Models ranged from a simple model with animals as the only random effect to a model that had maternal environmental effects as additional random effects as well as direct maternal genetic covariance. Fixed effects in the model comprised of herd, birth year, calving order and sex. Likelihood ratio test was used to determine the best fit model for the data. Results indicated that allowing for direct-maternal genetic covariance increases the direct and maternal effect dramatically. The best fit model had direct and maternal genetic effects as the only random effect with non-zero direct-maternal genetic correlation. Direct heritability, maternal heritability and direct maternal correlation of the best fit model was 0.57, 0.16 and -0.78 respectively. The result indicates that problem of (co-)sampling variation occurs when attempting to partition additive genetic variance into direct and maternal components.

scholarly journals 409 Effects of essential oils on in vitro ruminal fermentation using a high-concentrate substrate

2019 ◽  
Vol 97 (Supplement_3) ◽  
pp. 165-165
Author(s):  
Nadira J Espinoza-Rock ◽  
Andrea O Doblado ◽  
Sebastian E Mejia-Turcios ◽  
Evandro Dias ◽  
Michael Sandes ◽  
...  
Keyword(s):  
Essential Oils ◽  
Fixed Effects ◽  
Block Design ◽  
Random Effect ◽  
Gas Production ◽  
Experimental Unit ◽  
Ruminal Fermentation ◽  
Cinnamic Aldehyde ◽  
Garlic Oil

Abstract A randomized complete block design was used to determine the effects of 4 concentrations of 4 essential oils (EO) on in vitro ruminal fermentation variables. In vitro fermentation consisted of 0.7 g of high concentrate substrate (86.7% DM) and 50 mL of 2:1 buffer:ruminal fluid inoculum incubated for 24 h for each batch (n = 3; separate days) Treatments were arranged as a 4 × 5 factorial. Factors included 4 EO (eugenol, cinnamic aldehyde, anethole, and garlic oil) at 5 concentrations (0, 10, 75, 200, and 400 mg/L of inoculum). Data were analyzed using the MIXED procedure of SAS with the fixed effects of EO, concentration, and their interaction, and random effect of day (block). Batch was considered the experimental unit. There was an interaction (P < 0.001) for total gas production, where a cubic effect (P ≤ 0.041) was observed for eugenol, cinnamic aldehyde, and anethole, and a quadratic effect (P = 0.001) was observed for garlic oil. No interactions (P > 0.05) were observed for in vitro OM digestibility (IVOMD) or CH4 production. There was an effect of EO (P < 0.001) on IVOMD, where eugenol reduced (P ≤ 0.007) digestibility compared with anethole and garlic oil, which promoted the greatest (P ≤ 0.029) IVOMD. Methane production (mmol/g OM fermented) was affected by EO (P < 0.001), where it was decreased (P ≤ 0.001) by garlic oil compared with all other EO. There was an interaction (P < 0.001) for H2S production (µmol/g OM fermented), where it was linearly decreased (P = 0.003) and linearly increased (P < 0.001) as concentrations of eugenol and garlic oil increased, respectively. These EO had contradictory impacts on in vitro ruminal fermentation, thus combining them could potentially improve multiple aspects of in vitro and in vivo fermentation.

scholarly journals PSIV-10 Preference evaluation of plant functional types grasses in calves

2019 ◽  
Vol 97 (Supplement_3) ◽  
pp. 225-226
Author(s):  
María A Vulliez ◽  
María Bruni ◽  
Ana I Trujillo ◽  
Martín Durante ◽  
Lucrecia Lezana ◽  
...  
Keyword(s):  
Repeated Measures ◽  
Fixed Effects ◽  
Mixed Model ◽  
Block Design ◽  
Neutral Detergent Fiber ◽  
Functional Types ◽  
Randomized Block Design ◽  
Functional Types Of Plants ◽  
Group B ◽  
Cut Leaves

Abstract The functional types of plants (PFT) ecological approach could be used to link the preferences of ruminants. In order to investigate if ruminant preferences were associated with this approach, three PFT groups were evaluated. A group “B”, represented by Paspalum urvillei (PU-B), “C” by Nassella neesiana (NN-C) and Sporobolus indicus (SI-C) and “D” by Andropogon lateralis (AL-D). In a randomized block design with repeated measures over time, two independent cafeteria trials (autumn-winter, A-W, and spring-summer, S-S) were carried out in calves (n = 8 for A-W; n = 4 for S-S; BW=240–270 kg) arranged in four pens. In three sessions of ten minutes each, calves received 120–160 gDM of fresh cut leaves from the different species. Dry matter intake (DMI), nitrogen intake (NI) and neutral detergent fiber intake (NDFI) were measured. Data were analyzed with a mixed model that included specie and session as fixed effects and blocks as a random. The statistical analyses were performed with SAS (University Edition), data is expressed as lsmeans and was considered to differ when Tukey test presented P < 0.05. No significant differences were observed between sessions. The DMI and NI were higher in NN-C and lower in AL-D (P < 0.01) during A-W. However, in S-S, higher DMI and NI were observed in AL-D and SI-C while NN-C presented the lower DMI and NI (P < 0.01). The FDNI did not show differences in A-W meanwhile in S-S was positively associated with DMI (Table1). Interestingly, when the season changed, it was observed a change in PFT preference, in A-W calves preferred NN-C whereas in S-S preferred AL-D. However, attributes associated with preferences were maintained in both stations, for instance, higher DMI was associated with higher NI. Additional research is required on the interactions between PFT approach and the preference of animals for different species.

scholarly journals Mineral metabolism of pregnant goats under feed restriction

2017 ◽  
Vol 57 (2) ◽  
pp. 290 ◽  
Author(s):  
Carla J. Härter ◽  
Lisiane D. Lima ◽  
Douglas S. Castagnino ◽  
Astrid R. Rivera ◽  
Alana M. Nunes ◽  
...  
Keyword(s):  
Fixed Effects ◽  
Mineral Metabolism ◽  
Block Design ◽  
Random Effect ◽  
Serum Levels ◽  
The Body ◽  
Feed Restriction ◽  
Mineral Density ◽  
Maternal Body ◽  
Sodium And Potassium

We examined the effects of feed restriction on calcium, phosphorus, magnesium, sodium and potassium metabolism in Oberhasli and Saanen goats during gestation. The 63 goats were distributed into groups that were divided into three levels of feed restriction (0%, 20% and 40% restriction) and slaughtered at different pregnancy stages (80, 110 and 140 days of gestation), in a randomised block design with a 2 × 3 × 3 factorial arrangement. The mineral balance was determined at ~80, 110 and 140 days of gestation. The serum levels of minerals and alkaline phosphatase activity were determined during pregnancy. Mineral retention in the maternal body, femur, empty uterus, mammary gland, fetus and fetal fluid was also determined during gestation. Bone mineral density was measured in the femur. Mixed models with days of gestation, levels of feed restriction, breed and their interactions as fixed effects and blocks as random effect were used for data analysis. In response to the reduction in feed intake, the maternal body uses its mineral reserves to maintain gestation. Physiological adjustments of the goats subjected to 20% feed restriction avoided a decrease in fetal mineral deposition. More severe feed restriction, however, compromised concentrations of phosphorus, sodium and potassium in the fetus, which were the main minerals used by the maternal body, whereas calcium and magnesium deposition in fetuses remained unaffected. At 40% feed restriction, the retention of all minerals in the body decreased, and the fetal dry mass was on average also less than those fetuses from goats without feed restriction. The fetal deposition of phosphorus, sodium and potassium was also lower during 40% restriction.

scholarly journals Phytate-phosphorus and phytase contents on the relative weight of organs, intestinal morphometry and performance of broilers

2016 ◽  
Vol 46 (10) ◽  
pp. 1858-1864
Author(s):  
Aiane Aparecida da Silva Catalan ◽  
Everton Luis Krabbe ◽  
Valdir Silveira de Avila ◽  
Letícia dos Santos Lopes ◽  
Ana Paula Nunes ◽  
...  
Keyword(s):  
Fixed Effects ◽  
Block Design ◽  
Relative Weight ◽  
Average Weight ◽  
Feed Conversion ◽  
Phytate Phosphorus ◽  
Randomized Block Design ◽  
Random Block ◽  
And Performance ◽  
Intestinal Morphometry

ABSTRACT: In order to evaluate the efficiency of phytase in diets with low and high phytate phosphorus (PP) 0content, as a consequence of wheat bran inclusion, on the relative weight of organs, intestinal morphometry and performance, three hundred and eighty-four male Cobb500 broilers were housed in metabolic cages. Animals were assigned into four treatments in a 2x2 factorial scheme in a randomized block design with eight replicates of 12 birds each. From 11 days of age birds received experimental diets, which consisted of: Diet low in PP; Diet low in PP with phytase (500FTU kg-1); Diet with a high PP and Diet with a high PP with phytase (500FTU kg-1). At 22 and 32 days of age two birds were slaughtered in order to collect gizzard, heart, liver, cecum, cloacal bursa, and at 32 days, a portion of the duodenum, jejunum and ileum was collected for morphometric evaluation. From 22 to 32 days of age average feed intake, average weight gain, average body weight and feed conversion ratio were also evaluated. Data were subjected to analysis of variance, fixed effects of diet and phytase and interaction between factors as well as the random block effects were tested. There was no significant interaction for the variables studied, concluding that phytase in diets with low or high phytate phosphorus content did not change the relative weight of organs, intestinal morphometrics and performance; only isolated effects were observed.

scholarly journals PSI-4 Effects of increasing metabolizable protein on starch digestion in lightweight beef cattle

2020 ◽  
Vol 98 (Supplement_3) ◽  
pp. 226-226
Author(s):  
Cierra Crowell ◽  
Sara Tondini ◽  
Miles Redden ◽  
Daniel W Shike ◽  
Joshua C McCann
Keyword(s):  
Fixed Effects ◽  
Rumen Fluid ◽  
Random Effect ◽  
Beef Steers ◽  
Starch Digestion ◽  
Stomach Tube ◽  
Metabolizable Protein ◽  
D 12 ◽  
Dietary Treatments

Abstract The objective was to evaluate the effect of increasing MP on starch digestion in lightweight beef steers. Greater provision of metabolizable protein (MP) may increase RUP and aid in pancreatic amylase production for optimum digestion of starch in the small intestine. Twelve crossbred steers were early weaned and used in a crossover design with two 18-day periods and 4 dietary treatments. Lightweight steers (BW = 153 kg) were randomly assigned to a treatment sequence for the 2 periods. Diets provided MP at: 0.59 (MP1), 0.69 (MP2), 0.85 (MP3), and 0.91 kg/d (MP4) based on observed DMI. Additional MP was provided through greater inclusion of blood meal. Feed and total feces were collected on d 12-18 to determine total tract starch digestion. Rumen fluid was sampled prior to feeding on d 17 via stomach tube to assess in vitro starch disappearance over 8 h for each steer. Data were analyzed using the Mixed procedure of SAS with fixed effects of treatment and period, and a random effect of steer. Starch intake was not affected (P = 0.18) by treatment with MP1 and MP4 having similar starch intake values (3.3 kg/d and 3.1 kg/d, respectively). In vitro starch disappearance and final in vitro pH were not affected (P ≥ 0.18) by increasing MP. Similarly, fecal starch output was not different (P = 0.65) among treatments with MP1 treated steers having 0.21 kg/d of starch output and MP4 steers having 0.18 kg/d of starch output. Low fecal starch output values resulted in high total tract starch digestion ranging from 90-96% and were not affected (P = 0.52) by treatment. Overall, increasing dietary MP did not affect ruminal in vitro or total tract starch digestion.

scholarly journals 76 Does supplemental protein and rumen-protected methionine improve performance and digestibility during late-gestation in beef cows?

2019 ◽  
Vol 97 (Supplement_3) ◽  
pp. 75-75
Author(s):  
Madeline M Collins ◽  
Michaela K S Lievre ◽  
Kaitlyn V J Lawson ◽  
Ira B Mandell ◽  
Anna-Kate Shoveller ◽  
...  
Keyword(s):  
Body Weight ◽  
Amino Acid ◽  
Block Design ◽  
Dietary Treatment ◽  
Beef Cows ◽  
Late Gestation ◽  
Isoleucine Leucine ◽  
Randomized Block Design ◽  
Cow Performance ◽  
Supplemental Protein

Abstract Methionine (MET) is likely the first limiting amino acid in low-protein forages fed to beef cattle during late-gestation. Therefore, the objective of this study was to determine if supplemental protein and rumen-protected MET improve cow performance and apparent tract digestibility (TTD) during late-gestation. This study used 147 late-gestation Angus crossbred cows and heifers in a 3x2 factorial arrangement for dietary treatments. The cattle were randomly assigned to one of six diets formulated to 90, 100 or 110% of metabolizable protein (MP) requirements (NRC, 2016), with (without) 9 g/d of rumen-protected MET (MetaSmart, Adisseo Inc.). These diets were fed for approximately 8 wks before calving. All data was analyzed as a randomized block design using SAS 9.4 PROC GLIMMIX procedure. Cows fed at 90% MP requirements lost body weight (BW), while cows fed at 100% and 110% MP requirements maintained and (or) gained BW over the trial (P = 0.02). Similarly, cows fed at 90% MP requirements lost more pregnancy corrected BW than cows fed at 100% and 110% MP requirements (P = 0.01). However, supplemental MET did not affect body weight gains (P > 0.07). Cows and heifers fed at 90% MP requirements had reduced TTD for crude protein compared to cattle fed to 110% MP requirements (P < 0.001), MET supplementation did not impact TTD (P > 0.20). Additionally, cattle fed at 90% MP requirements had increased serum cholesterol and reduced urea concentrations compared to cows fed to 100% and 110% MP requirements (P < 0.0001). MET supplementation increased serum concentrations for glucose, isoleucine, leucine, lysine, serine, threonine and valine (P < 0.02). Calf birth weights were not significantly impacted by dietary treatment (P > 0.31). Feeding cows above their MP requirements may improve late-gestation performance and CP digestibility. Supplemental MET may increase amino acid utilization but did not improve beef cow performance or digestibility parameters measured in late-gestation.

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