Evolution of subterranean clover in South Australia. II. Change in genetic composition of a mixed population after 19 years' grazing on a commercial farm

1982 ◽  
Vol 33 (4) ◽  
pp. 679 ◽  
Author(s):  
PS Cocks ◽  
AD Craig ◽  
RV Kenyon

Change in genetic composition of a mixed population of subterranean clover was studied at Kalangadoo in the south-east of South Australia. The seeds from an experiment in which 25 late-maturing strains were compared, were sampled in January 1977, 19 years after the plants were originally sown in 1958. Quadrats along three transects over the approximate position of the old plots were sampled and seed yields measured. The soil was analysed for several chemical and physical properties at the site of each quadrat. Thirty seeds from each sample were germinated in a glasshouse and the resulting seedlings planted out as spaced plants. These were identified on the basis of flowering time and morphological variables; in this way the seed pool of each strain at each quadrat site was determined. Seed pools were adjusted for variation in distance of original plots from the transects by estimating the real position of each plot relative to the transect and calculating the relationship between seed yield and estimated distance. Highest adjusted seed pools were produced by CPI 14454B (820 kg ha-1), CPI 12695 (626 kg ha-1), Eden Valley (502 kg ha-1), and CPI 14197A (476 kg ha-1). Cultivars Mt Barker and Tallarook were also common, but it was deduced from their distribution that they had been continuously introduced to the site from surrounding fields by livestock. All but six of the original strains were recovered in the sampling. Also present were many divergent strains, which yielded 228 kg ha-1 or 31 % of the average seed yield of 744 kg ha-1. Twenty-four of the original 25 strains and five others-Woogenellup, Yarloop, Trikkala, Clare and a divergent strain naturalized at Kalangadoo-were sown in small plots. Twenty-five agronomic variables were measured, including seasonal herbage yields, petiole lengths, leaf areas, isoflavone contents, phosphorus and sulfur uptakes, above- and below-ground seed yields, seed and seedling sizes and permeability of seed coats. The fourth component of a principal component analysis of the data was related to seed pool (P < 0.001), and it is suggested that the variables contributing to this component are important in predicting the success of a strain at Kalangadoo. The most successful tended to have high yields of buried seed, high yields of herbage and long petioles in winter, high formononetin content, early maturity and small seedlings. The results are discussed in terms of factors affecting success, generation of new genetic diversity, and field implications.

1967 ◽  
Vol 7 (24) ◽  
pp. 25 ◽  
Author(s):  
GB Taylor ◽  
RC Rossiter

Seed production and persistence of the Carnamah, Northam A, Dwalganup, and Geraldton strains of subterranean clover (Trifolium subterraneum L.) were examined in undefoliated swards in the wheatbelt of Western Australia. The early flowering characteristic of Carnamah was not always associated with higher seed yields. Only when there was a well-defined, early finish to the growing season, or when flowering was very much earlier in Carnamah (viz., following an early 'break' to the season), did this strain clearly outyield both Northam A and Geraldton. The seed yield of Dwalganup was generally inferior to that of the other strains. Factors affecting regeneration are discussed. Under low rainfall conditions, poorer germination-regulation of Carnamah, compared with Geraldton and Northam A, would be expected to result in poorer persistence unless offset by higher seed yields in the Carnamah strain.


1981 ◽  
Vol 32 (5) ◽  
pp. 783 ◽  
Author(s):  
WJ Collins

The effects of length of growing season and defoliation on seed yield and hard-seededness were examined in two strains of subterranean clover (Seaton Park, Midland B) grown in swards in the field. All plots were sown at the same time and the length of growing season was varied by altering the time of finish of the season (by withholding water). There were three length of growing season treatments: T1 (short), T2 (intermediate) and T3 (control). The defoliation treatments were D0, uncut (control), and D1, defoliated at weekly intervals until the commencement of flowering. Reducing the length of growing season drastically reduced seed yield. Thus when the growing season was only 3 weeks shorter than the control (i.e. T2 compared with T3), seed yields averaged over strains and defoliations were reduced by at least one half. With a further reduction of 2 weeks in the length of the season (T1) seed yields were only about one-third of those obtained in the control (T3). The reductions in seed yield were due to reductions in both the number of mature burrs produced and to a lesser extent in mean weight per seed. Although defoliation increased seed yield in all growing seasons, the effect when measured on a relative scale was greater in T2 than in either T1 or T3. But on an absolute scale the size of the response was greater in T2 and T3 than in T1. The rate of breakdown of hard-seededness was faster in Seaton Park than in Midland B, but it decreased in both strains with increasing length of growing season.


1978 ◽  
Vol 29 (6) ◽  
pp. 1167 ◽  
Author(s):  
WJ Collins ◽  
RC Rossiter ◽  
MA Ramas

Swards of three strains of subterranean clover (Woogenellup, Clare and Seaton Park) were subjected to three shading treatments (30%, 55% and 100% of daylight) from the beginning of flowering onwards. For the 100% daylight (unshaded) treatment the incoming daily solar radiation averaged between 20 and 30 MJ/m2. In the unshaded treatment, seed yields for the three strains ranged from about 120 to almost 200 g/m2. These yields were invariably decreased by shading. Moreover the evidence indicated a curvilinear relationship between seed yield and light supply such that at 50% of daylight there was a reduction of rather more than 50% in seed yield. Shading mainly affected the number of inflorescences produced per unit area, although other components of seed yield were also affected adversely. Even late shading (towards the end of flowering) led to lower seed yield, partly associated with lower inflorescence numbers and partly with lower seed numbers per burr. The results are considered to have significance for clover regeneration in mixed clover/grass pastures and also in the cloveritree associations of agro-forestry.


1977 ◽  
Vol 17 (88) ◽  
pp. 765 ◽  
Author(s):  
AP Meissner ◽  
AL Clarke

Trials lasting three years were conducted at 27 sites in the south-east of South Australia to examine the responses of mown subterranean clover-ryegrass pastures to repeated annual applications of potassium chloride. At 20 sites, yield of dry matter (DM) was increased by potassium in one or more harvests. Responses were measured in 9 out of 55 winter harvests and 42 out of 86 spring harvests; mean responses to 100 kg ha-1 KCl (-Y100) at responsive sites were 380 and 560 kg DM ha-1 in winter and spring respectively. When potassium dressing increased yield, the proportion of clover in the sward was either maintained or increased. The herbage concentration of potassium was usually increased, and its magnesium concentration sometimes depressed, by potassium application. Although the K:Mg ratio was thereby increased, especially during winter, it rarely reached 15, the value considered necessary to induce grass tetany in cattle. Multivariate regression analysis showed that the exchangeable potassium concentration (Kex) of the top 10 cm of soil, the depth of soil overlying clay or limestone, and the sand content of the topsoil were factors affecting yield response. The best regression equations, however, explained less than half of the response variations. In practice, Kex alone would have usefully diagnosed potassium responsive sites. Responses occurred often when Kexwas less than 0.2 me 100 g-1 and rarely when it was greater. The use of potassium fertilizer is likely to be worthwhile to establish new pasture, to maintain the clover content of old pasture, and to increase the yield and quality of hay, where soils have less than 0.20 me 100 g-1 Kex. Such soils are most commonly sands more than 30 cm deep.


1985 ◽  
Vol 25 (3) ◽  
pp. 595 ◽  
Author(s):  
MDA Bolland

In three experiments located near Esperance, Western Australia, the effect of superphosphate phosphorus on seed yields of subterranean clover, serradella and annual medics was measured on newly-cleared soils, using low seeding rates. In two experiments, the relationship between seed yield and the amount of phosphorus applied was linear for subterranean clover and serradella; seed yields increasing by 7-24 kg/ha for each kg/ha of phosphorus applied, depending on species, strain or cultivar, and location. In the third experiment, seed yields of annual medic species also increased markedly with increasing amounts of applied superphosphate phosphorus, this response also depended on species and strain or cultivar, but the responses become less marked with increasing amounts of phosphorus. For the annual medic species, the phosphorus treatments had no effect on average weight of one burr, number of seeds per burr, weight of one seed, or the rate of softening of hard seeds as measured both in a 15/60� alternating temperature oven (one cycle/day) or for samples of burrs collected periodically during summer from the field. For all legumes, the appearance of first flowers was not affected by phosphorus treatment.


1985 ◽  
Vol 25 (4) ◽  
pp. 893 ◽  
Author(s):  
MDA Bolland

Most of the sandy soils near Esperance, W.A., were cleared of native vegetation and sown to subterranean clover in the 1950s and 1960s. Over the past 20-30 years, pH values of the topsoil (1:5, soil: water) have decreased from 6.5-7.0 to 5.0-5.5. The application of 2 t/ha of agricultural lime increased the soil pH (1:5, 0.01 CaCl2) from 5.5 to 5.8, from 4.9 to 5.6, and from 5.1 to 5.5 at three sites that had been sown to subterranean clover for 10, 20 and 40 years respectively. However, lime had no effect on either dry matter production or seed yield of subterranean clover. Additions of calcium in a further experiment also did not affect the seed yield of subterranean clover. Irrespective of the lime treatments, the addition of nutrients (phosphorus, potassium, sulfur, copper, zinc, molybdenum, cobalt, manganese and boron) significantly (P<0.05) improved dry herbage yields by a factor of 1.3, 1.7 and 1.4 at the 10-, 20- and 40-year-old sites. However, the addition of nutrients did not affect seed yields of subterranean clover. In other experiments, the omission of sulfur, potassium and, for the deepest sandy site, phosphorus reduced dry herbage yields by between 10-30%. The omission of molybdenum from treatments of the 20- and 40-year-old pasture reduced herbage yields by 21 and 16% respectively. However, omission of molybdenum in the experiment sited on the 10-year-old pasture did not affect yields. Thus, as the acidification of the sandplain soils continues, molybdenum may become deficient and may limit herbage yields of subterranean clover.


2006 ◽  
Vol 57 (4) ◽  
pp. 447 ◽  
Author(s):  
Ramakrishnan M. Nair ◽  
Steve J. Hughes ◽  
David M. Peck ◽  
Graham Crocker ◽  
Simon Ellwood ◽  
...  

Spotted medics (Medicago arabica) have become naturalised in Australia, but the spiny nature of their pods has prevented commercial release of any cultivar. Fifty-eight accessions representing Medicago arabica in the Australian Medicago Genetic Resources Collection were grown as spaced plants at Turretfield, South Australia, and the variation for important agronomic traits was studied. There was large variation for traits including days to flowering, dry matter production, pod and seed yield, and pod spininess. Principal component and cluster analyses conducted for 13 traits revealed 5 clusters. One of the clusters identified comprised accessions originating from Greece and Cyprus, which were found to have high agronomic potential. The study has helped in identifying the relationship among traits, namely pod spininess, days to flowering, dry matter yield, and pod and seed yield, which would be useful to breeders for future breeding and selection programs. A sward trial at Moree, New South Wales, comprising a selected cohort of spotted medic accessions, enabled the identification of 2 early flowering and high dry matter yielding accessions; however, both exhibited spiny pods. These 2 accessions were crossed with a smooth-podded accession, and the F1 plants were confirmed using a microsatellite marker. Days to flowering showed a continuous pattern of variation in the F2, suggesting that the trait is quantitatively inherited, whereas segregation ratio revealed that a single recessive gene controlled the smooth pod trait. Early flowering, smooth-podded F2 plants were selected for cultivar development.


2008 ◽  
Vol 48 (8) ◽  
pp. 1133 ◽  
Author(s):  
B. S. Dear ◽  
B. F. Hackney ◽  
G. M. Dyce ◽  
C. A. Rodham

Swards of four cultivars of subterranean clover (Trifolium subterraneum L.) were cut at three different times to determine the effect on forage yield and quality, seed set and seedling regeneration in two successive seasons in southern New South Wales. The four cultivars of subterranean clover (Seaton Park LF, Junee, Goulburn and Clare) were cut on 23–25 September (early cut), 8–10 October (mid cut) or 22–23 October (late cut), to simulate an early silage, late silage or hay cut. Additional treatments imposed included either grazing or leaving the regrowth after cutting and raising the cutting height from 3 to 6 cm. Forage yields ranged from 3.5 to 9.3 t dry matter (DM)/ha in the first year and from 2.0 to 5.9 t DM/ha in the second year. Herbage yield was influenced by both cultivar and harvest time with the highest yields achieved with the mid cut. Lower forage yields at the later cut were attributed to losses due to respiration and decay under dense leaf canopies. Changes in forage quality were consistent across both years, with in vivo DM digestibility declining from 76–79% to 69–70% as cutting time was delayed. Crude protein fell from 22–24% to 14–17% over the same period, depending on cultivar. Seed yields in both years were influenced by both cutting time and cultivar with a positive relationship (R2 = 0.45–0.61) between herbage present in late spring after a period of regrowth and subsequent seed yield. The early flowering cultivar Seaton Park LF had the highest seed yield in both years and the more erect cultivar Clare had the lowest. Seed yields declined with later cutting time but increased by an average of 39% when the cutting height was raised from 3 to 6 cm. Seedling regeneration reflected seed yield responses with the largest seedling regeneration occurring in treatments cut early. The study found that forage conservation in early October is likely to yield more and be of higher quality than swards cut later in the season. Seed set is greatly reduced by all cutting strategies to levels unprofitable for seed harvesting but may be adequate for pasture regeneration.


2015 ◽  
Vol 66 (9) ◽  
pp. 938 ◽  
Author(s):  
T. J. Ridsdill-Smith ◽  
C. C. Pavri

The use of a TIMERITE® spring spray to control redlegged earth mite (RLEM), Halotydeus destructor (Tucker) (Acari: Penthaleidae), in annual pastures was evaluated on farms across Australia. RLEM populations in autumn in the treatments sprayed in spring 1998 and 1999 were 97% lower in 1999 and 97% in 2000 in the western region (Western Australia), and 93% lower in 1999 and 93% in 2000 in the eastern region (Victoria, New South Wales and South Australia). At sites in the west, control of RLEM resulted in significant increases in subterranean clover seed yield in 1999 and in clover seedling numbers in autumn 1999 and 2000. Penthaleus major (blue oat mite) populations in autumn were 60% lower in sprayed treatments, but Sminthurus viridis (lucerne flea) populations were not affected. Differences in weather between the west (where there is a hot, dry summer) and the east (where temperature and rainfall regimes are more variable in spring and early summer) seem to cause greater RLEM control and greater benefits in subterranean clover seed yield and seedling numbers with a spring spray in the west.


1995 ◽  
Vol 35 (4) ◽  
pp. 475 ◽  
Author(s):  
GA Sandral ◽  
BS Dear ◽  
NE Coombes

The effect of broadleaf herbicides on seed set by Trifolium subterraneum (subterranean clover) cultivars was examined at 2 sites (Wagga Wagga and Canowindra) over 2 years. Five commonly used herbicide treatments (bromoxynil, MCPA, 2,4-DB, MCPA + terbutryn, MCPA + diuron) were applied at 2 rates to 7 cultivars of subterranean clover. Significant site x cultivar x herbicide interactions were observed. Seed yields were either unaffected or depressed by up to 66% at the higher rainfall site (Canowindra), whereas at the lower rainfall site (Wagga Wagga) some herbicide x cultivar combinations showed increases in seed yield up to 115%. The increase in seed yield was greater at the lower herbicide rate. The variation in seed yield with herbicide treatment was largely a result of a change (P<0.001) in the number of seeds set (R = 0.94 at Wagga Wagga; R = 0.85 at Canowindra). Seed size was also correlated (P<0.001) with seed yield at both sites but explained less of the variation (R = 0.23 at Wagga Wagga; R = 0.47 at Canowindra). The reduction in herbage yield as a result of herbicide application was a poor indicator of the subsequent seed yield response at both sites. Cultivar Trikkala was consistently most tolerant to the herbicide treatments, showing either no change in seed yield at the wetter site or large increases in seed yield at the lower rainfall site. In contrast, the seed yield of Dalkeith was depressed by 2,4-DB at both sites in both years by 39-66%. Increases in seed yield, which were most pronounced in cultivars of midseason maturity (Trikkala, Junee, Seaton Park) and least in later maturing cultivars (Karridale, Clare) and in the very early flowering Dalkeith, were attributed in part to a water-saving effect of the herbicide treatments. Cultivar maturity ranking was negatively correlated (P<0.001) with seed yield (R=-0.73 at Wagga Wagga; R=-0.45 at Canowindra). The germination percentage of seed produced by the cultivars was unaffected by herbicide treatment, although the number of abnormal radicles formed by germinating seed was higher in the 2,4-DB and MCPA treatments.


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