Digesta flow studies in sheep fed two mature annual legumes

1994 ◽  
Vol 45 (4) ◽  
pp. 889
Author(s):  
X Li ◽  
RC Kellaway ◽  
RL Ison ◽  
G Annison

Mature subterranean clover (Trifolium subterraneum cv. Junee) and Persian clover (Trifolium resupinatum cv. Kyambro) were fed to cannulated sheep. Flow rates of dry matter and nitrogen in the abomasum and ileum were estimated using a double marker method. Contents of hemicellulosic polysaccharides (xylose and mannose monomers) were higher in Kyambro than in Junee. Although Junee contained higher levels of total N and amino acids, the relative concentrations of cystine and methionine were markedly higher in Kyambro. Dry matter intakes and flow rates in the abomasum, ileum and faeces were similar on Kyambro and Junee. Dietary nitrogen intake was lower and endogenous re-cycled nitrogen higher on Kyambro than on Junee. Ileal flow of nitrogen was similar to nitrogen intake on Kyambro. Apparent nitrogen digestibility was lower in the whole tract and higher in the intestines on Kyambro. The higher apparent digestibility of nitrogen in the intestines of sheep eating Kyambro was associated with a greater proportion of nitrogen leaving the stomach coming from endogenous sources. The lower nitrogen digestibility of Kyambro in the whole tract indicated lower digestibility of dietary nitrogen, which was associated with higher contents of xylose and mannose.

2007 ◽  
Vol 47 (8) ◽  
pp. 927 ◽  
Author(s):  
M. D. A. Bolland ◽  
I. F. Guthridge

For the first time, we quantified pasture dry matter (DM) responses to applied fertiliser nitrogen (N) for intensively grazed, rain-fed, dairy pastures on sandy soils common in the Mediterranean-type climate of south-western Australia. The pastures are composed of subterranean clover (Trifolium subterraneum L.) and annual and Italian ryegrass (Lolium rigidum Gaud. and L. multiflorum Lam.). Six rates of N, as urea (46% N), were applied to 15 m by 15 m plots four times during 2002 and after each of the first 5–7 grazings in 2003 and 2004, throughout the typical April–October growing season. Total rates of N applied in the first year of the experiments were 0, 60, 120, 160, 200 and 320 kg N/ha, which were adjusted in subsequent years as detailed in the ‘Materials and methods’ section of this paper. The pastures in the experiments were rotationally grazed, by starting grazing when ryegrass plants had 2–3 leaves per tiller. The amount of pasture DM on each plot was measured before and after each grazing and was then used to estimate the amount of pasture DM consumed by the cows at each grazing for different times during the growing season. Linear increases (responses) of pasture DM to applied N occurred throughout the whole growing season when a total of up to 320 kg N/ha was applied in each year. No maximum yield plateaus were defined. Across all three experiments and years, on average in each year, a total of ~5 t/ha consumed DM was produced when no N was applied and ~7.5 t/ha was produced when a total of 200 kg N/ha was applied, giving ~2.5 t/ha increase in DM consumed and an N response efficiency of ~12.5 kg DM N/kg applied. As more fertiliser N was applied, the proportion of ryegrass in the pasture consistently increased, whereas clover content decreased. Concentrations of nitrate-N in the DM consistently increased as more N was applied, whereas concentrations of total N, and, therefore, concentration of crude protein in the DM, either increased or were unaffected by applied N. Application of N had no effect on concentrations of other mineral elements in DM and on dry matter digestibility and metabolisable energy of the DM. The results were generally consistent with findings of previous pasture N studies for perennial and annual temperate and subtropical pastures. We have shown that when pasture use for milk production has been maximised in the region, it is profitable to apply fertiliser N to grow extra DM consumed by dairy cows; conversely, it is a waste of money to apply N to undergrazed pastures to produce more unused DM.


1956 ◽  
Vol 7 (2) ◽  
pp. 98 ◽  
Author(s):  
JN Black

Changes in the pre-emergence distribution of dry matter in subterranean clover (Trifolium subterraneum L.) variety Bacchus Marsh were followed at 21°C, using three sizes of seed and three depths of sowing, ½, 1¼, and 2 in. Decreasing seed size and increasing depth of sowing both reduce the weight of the cotyledons a t emergence. Seed of the three sizes were sown a t three depths in pot culture a t staggered intervals so that emergence was simultaneous. Dry weight in the early vegetative stage was proportional to seed size, and total leaf area and leaf numbers showed similar trends. Plants of each seed size grew at the same relative rate. No effect of depth of sowing could be detected, and this was shown to be due to the cotyledon area a t emergence being constant for any given seed size, regardless of varying depth of sowing and hence of cotyledon weight. It was concluded that seed size in a plant having epigeal germination and without endosperm is of importance: firstly, in limiting the maximum hypocotyl elongation and hence depth of sowing, and secondly, in determining cotyledon area. Cotyledon area in turn influences seedling growth, which is not affected by cotyledon weight. Once emergence has taken place, cotyledonary reserves are of no further significance in the growth of the plants.


1972 ◽  
Vol 27 (1) ◽  
pp. 39-50 ◽  
Author(s):  
J. C. Macrae ◽  
M. J. Ulyatt ◽  
P. D. Pearce ◽  
Jane Hendtlass

1. In two experiments, sheep prepared with a rumen cannula and with re-entrant cannulas in the duodenum and ileum were continuously fed on diets of dried grass, dried grass plus formalin-treated casein, or dried grass plus untreated casein. Paper impregnated with chromic oxide was given once daily via the rumen fistula.2. In ten 24 h collections of digesta entering the duodenum and eleven 24 h collections of digesta reaching the ileum of sheep given dried grass, there were highly significant correlations between the 24 h flows of Cr marker and the corresponding flows of dry matter, organic matter, nitrogen, gross energy, hemicellulose and cellulose (P < 0.01) at both sites.3. Daily amounts of non-ammonia N and of individual amino acids entering and leaving the small intestine and of total N excreted in faeces and urine are given.4. Net retention of supplementary N was 36% when the supplement was administered as formalin-treated casein, but only 17% when it was administered as untreated casein.5. Formalin treatment of casein significantly increased the daily amounts of non-ammonia N entering the small intestine (P < 0.01) and the amounts of non-ammonia N apparently absorbed therein (P < 0.05).6. Apparent absorption of amino acids from the small intestine was significantly greater (P < 0.05) with treated casein than with untreated casein. There were relative increases in the small amounts of several free amino acids measured, including taurine, in the ileal digesta of sheep receiving the treated casein supplement.


2020 ◽  
Vol 51 (5) ◽  
pp. 2155-2159
Author(s):  
Rajabdeen Jannathulla ◽  
Rajarajan Yuvapushpa ◽  
Eugine Amala Claret ◽  
Gopalapillai Gopikrishna ◽  
Kondusamy Ambasankar ◽  
...  

1989 ◽  
Vol 113 (2) ◽  
pp. 149-163 ◽  
Author(s):  
M. F. Fuller ◽  
A. Cadenhead ◽  
D. S. Brown ◽  
A. C. Brewer ◽  
M. Carver ◽  
...  

SUMMARYEstimates were made of the dry matter (DM) yield and N content of cereal grains harvested from trial plots. They included 236 combinations of variety and treatment, with 13 varieties of winter wheat grown under six husbandry regimes, 14 varieties of spring barley under three husbandry regimes and ten varieties of winter barley under 11 husbandry regimes. The husbandry included timing and rate of fertilizer application, use of growth regulator and fungicide, and seed rate.For all three cereals, there were highly significant positive associations between grain N content (g/kg DM) and grain DM yield (t/ha) when individual varieties were grown under different conditions. The regression coefficients were: winter wheat 2·28, spring barley 0·74 and winter barley 1·06. When varieties were compared under constant husbandry, the association between N content and yield was negative; the regression coefficients were: winter wheat – 1·53, spring barley –1·14 and winter barley –1·21.The contents of lysine, threonine, isoleucine and valine were examined in 19 samples of winter wheat, 19 of spring barley and 21 of winter barley. Each cereal type included a comparison of varieties under one husbandry regime and a comparison of husbandry regimes in one variety.The amino acid composition of wheat protein changed little with either variety or treatment. In spring and winter barley, lysine concentration in grain protein (g/16 g N) decreased with increases in grain N which arose from additional fertilizer N. There were similar decreases in threonine, isoleucine and valine in winter barley, but varietal differences in grain N were not associated with significant changes in the amino acid composition of grain protein.The nutritive values of spring barley, winter barley and winter wheat were compared in digestion and N balance studies in growing pigs. A subset often samples was examined which included, for each cereal type, high- and low-protein varieties, each (except for spring barley) grown with high or low rates of applied fertilizer N.Apparent digestibility of dry matter measured at the terminal ileum was similar (0·67–0·70) in all three types of cereal but there was a tendency for the DM of high-protein varieties to be digested better than that of low-protein varieties. Over the whole digestive tract, the apparent digestibility of the DM of wheat was higher (0·83) than that of barley (0·75).The apparent digestion of the starch of all samples was virtually complete (0·98) by the end of the ileum; the remainder was digested in the large intestine.The apparent digestibility of N up to the terminal ileum was significantly higher for wheat than for barley, and significantly higher in high- than in low-protein varieties. Over the whole gastro-intestinal tract, the difference between the cereals was even larger; a greater proportion of wheat N than of barley N was digested in the large intestine.The rate of N fertilizer application did not significantly affect the apparent digestibility of any amino acid but there were significant differences amongst the cereal types in the apparent digestibility of seven amino acids. The amino acids in high-protein varieties were, on average, 6 % more digestible than those in low-protein varieties.When allowance was made for the endogenous flow of amino acids, some of the differences between cereals and between high- and low-protein varieties disappeared but some significant differences remained.The biological value (BV) of wheat protein (0·43) was significantly lower than that of barley protein (mean 0·57). When pigs were fed wheat as the sole protein source, the higher N content of wheat compensated for its lower BV. There were no significant differences in the rates of N retention between pigs fed on wheat or barley.


1991 ◽  
Vol 31 (6) ◽  
pp. 777
Author(s):  
MDA Bolland

The effect of superphosphate applications (0, 25, 50, 75, 100 and 125 kg P/ha to the soil surface) on the dry matter (DM) herbage production of dense swards of subterranean clover (Trifolium subterraneum cv. Junee) and yellow serradella (Ornithopus compressus cv. Tauro) was measured in a field experiment on deep, sandy soil in south-western Australia. The swards were defoliated with a reel mower at weekly intervals from 88 to 158 days after sowing, to a height of 2 cm for the first 9 cuts, 4 cm for the tenth cut and 5 cm for the eleventh cut. Yellow serradella was more productive than subterranean clover. Consequently, for the relationship between yield and the level of phosphorus (P) applied, yellow serradella supported larger maximum yields and required less P than subterranean clover, to produce the same DM herbage yield. Maximum yields of yellow serradella were 12-40% larger. To produce 70% of the maximum yield for yellow serradella at each harvest, yellow serradella required about 50% less P than subterranean clover. However, when yields were expressed as a percentage of the maximum yield measured for each species at each harvest, the relationship between yield and the level of P applied was similar for both species, and they had similar P requirements.


1998 ◽  
Vol 49 (1) ◽  
pp. 53 ◽  
Author(s):  
C. Tang ◽  
L. Barton ◽  
C. Raphael

The capacity of subterranean clover (Trifolium subterraneum L. cv. Clare), medic (Medicago murex Willd. cv. Zodiac), serradella (Ornithopus sativus Brot. line SP1/13), biserrula (Biserrula pelecinus L. line Mor99), and woolly clover (Trifolium tomentosum L.) to acidify soil under N2 fixation was compared in a pot experiment using a poorly buffered sandy soil. The amount of acid produced per kg shoot dry matter (specific acid production) varied betweefin species and with growth stages, ranging from 44 to 128 cmol/kg shoot. Subterranean clover and serradella acidied soil to a greater extent than woolly clover and medic, whereas biserrula acidified soil least. Irrespective of pasture species and growth stage, specific acid production correlated well with concentrations of excess cations and calcium in shoots. Furthermore, total excess cation, ash alkalinity, and calcium in shoots were all good indicators of total acid production across all of the species.


2001 ◽  
Vol 41 (2) ◽  
pp. 169 ◽  
Author(s):  
Y. J. Ru ◽  
J. A. Fortune

The nutritive value of 26 cultivars of dry, mature subterranean clover was evaluated at Shenton Park, Perth, Western Australia. The cultivars were divided into 3 maturity groups according to flowering time and each cultivar was sown in blocks comprising 4 replicates. The plots were grazed by sheep at 2-week intervals during the growing season. Dry mature plant material and soil were sampled in summer to examine the effect of grazing and cultivar on seed yield and nutritive value of feed residues. Cultivars heavily grazed in spring had a low herbage mass. There was no difference in seed yield and seed weight between heavily and lightly grazed cultivars. Dry matter digestibility and mineral content of dry residues was inconsistent for the 2 grazing treatments. The dry matter digestibility of dry, mature subterranean clover ranged from 40 to 56%, with a wide range of crude fibre, nitrogen and mineral content for the 26 cultivars. While most minerals in the dry residues were above the requirement for sheep, 7 cultivars had a zinc content less than the maintenance requirement for sheep. There was an imbalance for all cultivars in calcium: phosphorus with a range of 4–10: 1. Concurrent estimates on the yield and composition of seed indicated that seed can be resource of minerals for grazing animals in summer. Most cultivars had a seed yield over 100 g/m2 with that of 9 cultivars being over 130 g/m2. Seed was rich in nitrogen, sodium, phosphorus, potassium, magnesium, zinc and copper, and poor in sodium, calcium and manganese. However, there were no cultivars with an appropriate ratio of calcium and phosphorus. The imbalance in nitrogen and sulfur was a result of high nitrogen content with the ratio ranging from 19: 1 to 29: 1.


1973 ◽  
Vol 13 (64) ◽  
pp. 556
Author(s):  
DW Barrett ◽  
GW Arnold ◽  
NA Campbell

Pastures containing subterranean clover (Trifolium subterraneum) and either Vulpia spp. or Bromus rigidus as the other major species were sprayed at 0, 0.07, 0.14 and 0.21 kg a.i. ha-1 of paraquat ion between June and early October in Western Australia. Spraying removed the grasses and produced pastures containing up to 95 per cent clover. Mid-winter applications were more effective in increasing clover content than those made in spring. These changes in botanical composition were evident in the year following spraying, but were less marked. Yields of dry matter were reduced by paraquat, especially 'in the period immediately following spraying. These losses tended to decline as the growing season progressed, but at the close they were still evident on the Bromus rigidus pasture sprayed in July. Yields at the end of the subsequent season were similar on all treatments. Paraquat applied in mid-August at 0.14 kg a.i. ha-1 to both pastures produced the greatest change in botanical composition with the minimum loss of yield. The concentrations of nitrogen, phosphorus, calcium and magnesium were higher in mature herbage on paraquat treatments. Total yields of nutrients were similar between treatments because of reduced dry matter yield.


1972 ◽  
Vol 12 (59) ◽  
pp. 608 ◽  
Author(s):  
RC Rossiter ◽  
GB Taylor ◽  
GW Anderson

Pasture swards of the annual clovers Trifolium subterraneum (CV. Geraldton), T. hirtum (CV. Kondinin), and T. cherleri (CV. Yamina), and a mixture of all three were sown on a gravelly soil at Bakers Hill, Western Australia, in 1964. All pastures were set-stocked at 5 sheep ha-l from April 1965-April 1968, and at 8 sheep ha-1 from April 1968-March 1970. From 1966 onwards the amount of pasture dry matter on offer in July and September was two to four times as great on the subterranean clover pasture as on rose or cupped clover pastures. Moreover, the subterranean clover pasture resisted invasion by volunteer annuals more strongly. The mixed clover pasture was dominated by subterranean clover by 1966, and remained so thereafter. For the first four seasons of grazing, wool production was consistently higher (on average, 14 per cent higher) on rose clover than on subterranean clover pasture, and slightly lower still on cupped clover. In the fifth season, when grazing pressure was higher than previously, the rose and cupped clovers 'crashed', and subterranean clover produced most wool. We argue that the higher wool production on rose clover during 1965-1969 was due to some factor additional to digestible organic matter intake.


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