Studies of seed pelleting as an aid to legume inoculation. 5. Effects of incorporation of molybdenum compounds in the seed pellet on inoculant survival, seedling nodulation and plant growth of lucerne and subterranean clover

1980 ◽  
Vol 20 (102) ◽  
pp. 63 ◽  
Author(s):  
RR Gault ◽  
J Brockwell
Keyword(s):  
Nitrogen Fixation ◽  
Plant Growth ◽  
Nitrogen Content ◽  
Significant Relationship ◽  
Storage Time ◽  
Sodium Molybdate ◽  
Subterranean Clover ◽  
Ammonium Molybdate ◽  
Molybdic Acid ◽  
Molybdenum Disulphide

Four molybdenum compounds were mixed with lime and applied as coatings to inoculated seed of lucerne (Hunter River) and subterranean clover (Mount Barker). The seed was sown immediately in molybdenum-deficient soil in the field or stored for periods up to 84 days before sowing. As storage time lengthened, the survival of both lucerne and clover rhizobia was adversely affected by sodium molybdate but not by molybdic acid, ammonium molybdate or molybdenum disulphide. This effect was reflected in poorer nodulation in the sodium molybdate treatments. Nitrogen fixation, using foliage nitrogen content as an index, was always higher in the molybdenum treatments than in the no-molybdenum controls. Both species appeared able to extract molybdenum from molybdenum disulphide. Otherwise, there were no treatment differences in plant growth, but there was a significant relationship between the proportion of seedlings nodulated by the inoculant strains and the amount of nitrogen fixation. It is concluded that seed-applied molybdenum would benefit pasture establishment in some circumstances and would not interfere with inoculant survival or seedling nodulation provided that sodium molybdate was not used for the purpose.

The effect of Inoculation and cobalt application on the growth of and nitrogen fixation by sweet lupins

1978 ◽  
Vol 29 (6) ◽  
pp. 1191 ◽  
Author(s):  
DL Chatel ◽  
AD Robson ◽  
JW Gartrell ◽  
MJ Dilworth
Keyword(s):  
Nitrogen Fixation ◽  
Cell Division ◽  
Plant Growth ◽  
Nitrogen Content ◽  
Lupinus Angustifolius ◽  
Fixed Nitrogen ◽  
Soil Application ◽  
Seed Inoculation

The response of sweet lupins, Lupinus angustifolius L., to a soil application of cobalt and to seed inoculation was examined in both field and glasshouse experiments. Plant growth was dependent on nodule-fixed nitrogen, and the addition of cobalt increased the nitrogen content and the growth of the lupins in the absence of inoculation. Bacteroids in the nodules of inoculated plants without cobalt were found to be fewer and longer than those with cobalt, which suggests that cobalt is involved in the mechanism of rhizobial cell division.

The cobalt requirement of subterranean clover in the field

1963 ◽  
Vol 14 (1) ◽  
pp. 39 ◽  
Author(s):  
PG Ozanne ◽  
EAN Greenwood ◽  
TC Shaw
Keyword(s):  
Nitrogen Fixation ◽  
Nitrogen Content ◽  
Symbiotic Nitrogen Fixation ◽  
Subterranean Clover ◽  
Applied Nitrogen

Yield increases of 30% were obtained on two subterranean clover pastures in response to dressings of 2 and 10 oz CoSO4.7H2O per acre. A dressing of salts containing chromium, nickel, vanadium, tungsten, aluminium, and iodine had no effect. Applications of cobalt increased the nitrogen content of the clover in all cases. No response to cobalt was obtained in the presence of adequate applied nitrogen. Clover growth was sharply reduced when cobalt contents fell below 0.04 p.p.m. The unfertilized soils on which the experiments were located contained only 0.022 and 0.019 p.p.m. cobalt in the 0–4 in. layer. Applied cobalt was not leached downward but remained in the surface 4 in. However, less than 0.5% of the applied cobalt was taken up by the pasture. To obtain a response to applied cobalt it appears necessary for legumes to be growing in soil containing Rhizobia capable of symbiotic nitrogen fixation; but the soil must also be very low in available cobalt and nitrogen.

The control of molybdenum deficiency in subterranean clover by pre-soaking the seed in sodium molybdate solution

1951 ◽  
Vol 2 (3) ◽  
pp. 295 ◽  
Author(s):  
CM Donald ◽  
D Spencer
Keyword(s):  
Nitrogen Content ◽  
Maximum Yield ◽  
Sodium Molybdate ◽  
Subterranean Clover ◽  
Normal Growth ◽  
Direct Application ◽  
Yield Response ◽  
Test Plant ◽  
Cent Sodium ◽  
Pot Culture

Pre-soaking seed in a sodium molybdate solution was compared with the direct application of sodium molybdate to the soil as a means of preventing deficiency of molybdenum. Subterranean clover, which was used as the test plant, was grown in a molybdenum deficient soil in pot culture for this study. Pre-soaking in 0.1 per cent. or 1 per cent. sodium molybdate was fully effective in promoting normal growth. The soil and seed treatments were equally effective in promoting yield response at all levels of application examined. At levels of application in excess of the requirements for maximum yield, pre-soaking gave a higher molybdenum content of the tops than did equivalent soil applications. At all levels of application, pre-soaking gave a higher nitrogen content than that resulting from the soil application of molybdenum.

The effect of sodium molybdate mixed in the lime seed pellet on nodulation, nitrogen content, and growth of subterranean clover

1969 ◽  
Vol 9 (39) ◽  
pp. 432 ◽  
Author(s):  
JW Gartrell
Keyword(s):  
Nitrogen Content ◽  
Dry Matter ◽  
Sodium Molybdate ◽  
Subterranean Clover ◽  
Trifolium Subterraneum ◽  
Growing Season ◽  
Combined Nitrogen ◽  
Matter Production ◽  
Clover Seed ◽  
Ground Limestone

Sodium molybdate at l 1/2 oz, 3 oz, and 6 oz mixed with 6 lb ground limestone and pelleted onto 12 lb of Trifolium subterraneum cv. Geraldton inoculated with peat culture markedly reduced clover nodulation, growth, and combined nitrogen production compared with nil sodium molybdate. Mixtures of sodium molybdate l 1/2 oz, 3 oz, and 6 oz with l80 lb superphosphate drilled at 180 lb an acre with 12 lb an acre clover seed inoculated and pelleted with 6 lb ground limestone had no effect on nodulation, increased dry matter production by 70 per cent, and increased nitrogen per acre in tops by 110 per cent compared with nil sodium molybdate. The differences persisted into the second growing season.

Involvement of Phosphorus in Nitrogen Fixation by Subterranean Clover (Trifolium subterraneum L.)

1981 ◽  
Vol 8 (5) ◽  
pp. 427 ◽  
Author(s):  
AD Robson ◽  
GW O'hara ◽  
LK Abbott
Keyword(s):  
Nitrogen Fixation ◽  
Plant Growth ◽  
Phosphorus Deficiency ◽  
Mycorrhizal Fungus ◽  
Subterranean Clover ◽  
Maximum Growth ◽  
Nodule Formation ◽  
Growth Responses ◽  
Nitrogen And Phosphorus ◽  
Positive Interaction

Effects of phosphorus supply on nodulation and nitrogen fixation in subterranean clover paralleled those on growth and occurred only after, or at the same time as, growth responses. However, correcting phosphorus deficiency increased nitrogen concentrations in tops as well as weight of tops. Effects of vesicular-arbuscular (VA) mycorrhizas on growth, nodulation and nitrogen fixation operated through effects on phosphorus nutrition of the host. Inoculation with a VA mycorrhizal fungus only stimulated nodulation and nitrogen fixation when insufficient phosphorus was applied for maximum growth of the non-mycorrhizal plant. Phosphorus concentrations in nodules greatly exceeded those in either tops or roots in both mycorrhizal and non-mycorrhizal plants and with phosphorus levels ranging from severely deficient to luxury supply for plant growth. Nitrogen applied after nitrogen fixation had commenced increased fresh weight of tops to a greater extent where phosphorus was non-limiting to growth. This positive interaction between nitrogen and phosphorus on the growth of nodulated nitrogen-fixing subterranean clover suggests that phosphorus deficiency does not limit growth in legumes by decreasing nitrogen fixation. It is concluded that increasing phosphorus supply increases nitrogen fixation in subterranean clover by stimulating host plant growth rather than by effects on either rhizobial growth and survival or on nodule formation and function.

scholarly journals Foliar Application of Sodium Molybdate Enhanced Nitrogen Uptake and Translocation in Soybean Plants by Improving Nodulation Process Under Salt Stress

2017 ◽  
Vol 50 (3) ◽  
pp. 71-82 ◽  
Author(s):  
S. Farhangi-Abriz ◽  
R. Faegi-Analou ◽  
N. Nikpour-Rashidabad
Keyword(s):  
Salt Stress ◽  
Plant Growth ◽  
Nitrogen Content ◽  
Nitrogen Metabolism ◽  
Nitrogen Uptake ◽  
Foliar Application ◽  
Sodium Molybdate ◽  
Soybean Plants ◽  
Biological Nitrogen ◽  
Harmful Effects

Abstract Soil salinity with different harmful effects on plant growth and productivity is one of the main reasons in diminishing biological nitrogen fixation and nitrogen assimilation in legume plants. Molybdate has a key role on nitrogen metabolism of plants and can be has a beneficial effect on it. Thus, this experiment was conducted to evaluate the effects of sodium molybdate spraying (0.2 and 0.4% solutions in water) on nodulation, nitrogen uptake and translocation in soybean plants under different levels of salt stress (0, 5 and 10 dS m−1 NaCl, respectively). Salinity reduced the nodulation, root and shoot growth and special flavonoids content in roots, which are have a key role in nodulation includes, daidzein, genistein, coumestrol and glycitein, also diminished nitrogenase, glutamine synthetase (GS), glutamate dehydrogenase (GDH), glutamine oxoglutarate aminotransferase (GOGAT) and nitrate reductase (NR) activities in nodes, nitrogen content of nodes, roots and leaves, nitrogen uptake and translocation by soybean plants. Under salt stress and nonsaline condition, sodium molybdate treatments improved the nodulation by increasing flavonoids content of roots, also these treatments enhanced the plant growth and nitrogenase, GS, GDH, GOGAT and NR activities of nodes. Furthermore, nitrogen content of nodes, roots and leaves, nitrogen uptake and translocation by soybean plants improved by sodium molybdate applications. Both of the sodium molybdate doses, exposed the similar effects on improving nodulation and nitrogen metabolism of soybean.

A Study on the Farmers’ Awareness and Acceptance of Biofertilizers in Kottayam District

GIS Business ◽  
2019 ◽  
Vol 14 (6) ◽  
pp. 425-431
Author(s):  
Subin Thomas ◽  
Dr. M. Nandhini
Keyword(s):  
Organic Matter ◽  
Nitrogen Fixation ◽  
Plant Growth ◽  
Plant Growth Promoting Rhizobacteria ◽  
Nutrient Cycle ◽  
Plant Growth Promoting ◽  
Promote Plant Growth ◽  
Growth Promoting ◽  
Structured Questionnaire ◽  
Area Data

Biofertilizers are fertilizers containing microorganisms that promote plant growth by improving the supply of nutrients to the host plant. The supply of nutrients is improved naturally by nitrogen fixation and solubilizing phosphorus. The living microorganisms in biofertilizers help in building organic matter in the soil and restoring the natural nutrient cycle. Biofertilizers can be grouped into Nitrogen-fixing biofertilizers, Phosphorous-solubilizing biofertilizers, Phosphorous-mobilizing biofertilizers, Biofertilizers for micro nutrients and Plant growth promoting rhizobacteria. This study conducted in Kottayam district was intended to identify the awareness and acceptance of biofertilizers among the farmers of the area. Data have been collected from 120 farmers by direct interviews with structured questionnaire.

Growth and nitrogen fixation by subterranean clover in response to inoculation, molybdenum application and soil amendment with lime

1985 ◽  
Vol 17 (6) ◽  
pp. 791-796 ◽  
Author(s):  
D.R. Coventry ◽  
J.R. Hirth ◽  
T.G. Reeves ◽  
V.F. Burnett
Keyword(s):  
Nitrogen Fixation ◽  
Soil Amendment ◽  
Subterranean Clover

scholarly journals Effects of Biofertilizer Produced from Bradyrhizobium and Streptomyces griseoflavus on Plant Growth, Nodulation, Nitrogen Fixation, Nutrient Uptake, and Seed Yield of Mung Bean, Cowpea, and Soybean

Agronomy ◽  
2019 ◽  
Vol 9 (2) ◽  
pp. 77 ◽  
Author(s):  
Aung Zaw Htwe ◽  
Seinn Moh Moh ◽  
Khin Myat Soe ◽  
Kyi Moe ◽  
Takeo Yamakawa
Keyword(s):  
Nitrogen Fixation ◽  
Plant Growth ◽  
Seed Yield ◽  
Mung Bean ◽  
Growth And Yield ◽  
Vegetable Crops ◽  
N Application ◽  
Npk Uptake ◽  
Phosphorus And Potassium ◽  
Leguminous Crops

The use of biofertilizers is important for sustainable agriculture, and the use of nodule bacteria and endophytic actinomycetes is an attractive way to enhance plant growth and yield. This study tested the effects of a biofertilizer produced from Bradyrhizobium strains and Streptomyces griseoflavus on leguminous, cereal, and vegetable crops. Nitrogen fixation was measured using the acetylene reduction assay. Under N-limited or N-supplemented conditions, the biofertilizer significantly promoted the shoot and root growth of mung bean, cowpea, and soybean compared with the control. Therefore, the biofertilizer used in this study was effective in mung bean, cowpea, and soybean regardless of N application. In this study, significant increments in plant growth, nodulation, nitrogen fixation, nitrogen, phosphorus, and potassium (NPK) uptake, and seed yield were found in mung beans and soybeans. Therefore, Bradyrhizobium japonicum SAY3-7 plus Bradyrhizobium elkanii BLY3-8 and Streptomyces griseoflavus are effective bacteria that can be used together as biofertilizer for the production of economically important leguminous crops, especially soybean and mung bean. The biofertilizer produced from Bradyrhizobium and S. griseoflavus P4 will be useful for both soybean and mung bean production.

scholarly journals Genomic characterization and computational phenotyping of nitrogen-fixing bacteria isolated from Colombian sugarcane fields

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Luz K. Medina-Cordoba ◽  
Aroon T. Chande ◽  
Lavanya Rishishwar ◽  
Leonard W. Mayer ◽  
Lina C. Valderrama-Aguirre ◽  
...  
Keyword(s):  
Antibiotic Resistance ◽  
Nitrogen Fixation ◽  
Plant Growth ◽  
Phosphate Solubilization ◽  
Nitrogen Fixing ◽  
Nitrogen Fixing Bacteria ◽  
Promising Tool ◽  
A Genome ◽  
Plant Growth Promoting ◽  
Growth Promoting

AbstractPrevious studies have shown the sugarcane microbiome harbors diverse plant growth promoting microorganisms, including nitrogen-fixing bacteria (diazotrophs), which can serve as biofertilizers. The genomes of 22 diazotrophs from Colombian sugarcane fields were sequenced to investigate potential biofertilizers. A genome-enabled computational phenotyping approach was developed to prioritize sugarcane associated diazotrophs according to their potential as biofertilizers. This method selects isolates that have potential for nitrogen fixation and other plant growth promoting (PGP) phenotypes while showing low risk for virulence and antibiotic resistance. Intact nitrogenase (nif) genes and operons were found in 18 of the isolates. Isolates also encode phosphate solubilization and siderophore production operons, and other PGP genes. The majority of sugarcane isolates showed uniformly low predicted virulence and antibiotic resistance compared to clinical isolates. Six strains with the highest overall genotype scores were experimentally evaluated for nitrogen fixation, phosphate solubilization, and the production of siderophores, gibberellic acid, and indole acetic acid. Results from the biochemical assays were consistent and validated computational phenotype predictions. A genotypic and phenotypic threshold was observed that separated strains by their potential for PGP versus predicted pathogenicity. Our results indicate that computational phenotyping is a promising tool for the assessment of bacteria detected in agricultural ecosystems.

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