scholarly journals Effects of Biofertilizer Produced from Bradyrhizobium and Streptomyces griseoflavus on Plant Growth, Nodulation, Nitrogen Fixation, Nutrient Uptake, and Seed Yield of Mung Bean, Cowpea, and Soybean

Agronomy ◽  
2019 ◽  
Vol 9 (2) ◽  
pp. 77 ◽  
Author(s):  
Aung Zaw Htwe ◽  
Seinn Moh Moh ◽  
Khin Myat Soe ◽  
Kyi Moe ◽  
Takeo Yamakawa

The use of biofertilizers is important for sustainable agriculture, and the use of nodule bacteria and endophytic actinomycetes is an attractive way to enhance plant growth and yield. This study tested the effects of a biofertilizer produced from Bradyrhizobium strains and Streptomyces griseoflavus on leguminous, cereal, and vegetable crops. Nitrogen fixation was measured using the acetylene reduction assay. Under N-limited or N-supplemented conditions, the biofertilizer significantly promoted the shoot and root growth of mung bean, cowpea, and soybean compared with the control. Therefore, the biofertilizer used in this study was effective in mung bean, cowpea, and soybean regardless of N application. In this study, significant increments in plant growth, nodulation, nitrogen fixation, nitrogen, phosphorus, and potassium (NPK) uptake, and seed yield were found in mung beans and soybeans. Therefore, Bradyrhizobium japonicum SAY3-7 plus Bradyrhizobium elkanii BLY3-8 and Streptomyces griseoflavus are effective bacteria that can be used together as biofertilizer for the production of economically important leguminous crops, especially soybean and mung bean. The biofertilizer produced from Bradyrhizobium and S. griseoflavus P4 will be useful for both soybean and mung bean production.

Author(s):  
Fen Gao ◽  
Yuanhong Chen ◽  
SeaRa Lim ◽  
Allen Xue ◽  
Bao-Luo Ma

Effective nitrogen (N) management strategies are important for ensuring a balance between optimizing plant growth and minimizing disease damage. A field experiment was conducted for three years to (i) assess the effects of N fertilizer application on the growth and seed yield of canola, and severities of Sclerotinia stem rot (SSR), and (ii) determine a reasonable N-rate for optimizing plant growth and minimizing the loss from SSR in eastern Canada. The experiment was designed with factorial combinations of eight N treatments and two canola hybrids. All N-treatments reduced canola emergence with increasing preplant N application rates above 100 kg ha–1, but had a positive impact on plant height, fresh weight, dry weight and seed yield. The development of SSR showed differential responses to N application rates. Of all the treatments, the split application (50 kg N ha–1 at preplant plus 100 kg N ha–1 side-dressed at the 6-leaf stage) increased canola growth, and often produced the highest or similar seed yields to those of equivalent N rate applied as preplant. At the 150 kg ha–1 N rate, no severe development of SSR was observed in either preplant-only or split application. Overall, this study demonstrates that the split-N management strategy (50+100 kg ha–1) maintained a balance between enhancing plant growth and mitigating the negative impacts of SSR on canola.


2021 ◽  
Vol 11 (01) ◽  
pp. 736-748
Author(s):  
M. M. Hossain ◽  
◽  
S. Yesmin ◽  
M. Z. Islam ◽  
M. A. Hossain ◽  
...  

An experiment was conducted at the Sher-e-Bangla Agricultural University farm from April to June 2017 in Kharif season to evaluate the performance of two mungbean varieties under different NPK fertilizers doses in field conditions. The experiment was laid out in Randomized Complete Block Design (RCBD), comprising six treatments with three replications. In the experiment, varieties used were BARI mung-6 and BARI mung-5 and the combination of the treatment were T0 = (control), T1 (10-40-25 NPK kg ha-1), T2 (10-60-45 NPK kg ha-1), T3 (20-50-35 NPK kg ha-1), T4 (30-40-25 NPK kg ha-1) and T5 (30-40-45 NPK kg ha-1), respectively. Mungbean cultivars responded noticeably to the supplementary NPK fertilizers as the crop characters were significantly influenced by different levels of NPK fertilizers. Data were recorded on plant height, leaves plant-1, branches plant-1, pods plant-1, seeds pod-1, 1000-seed weight, seed, and straw yield at different days after sowing. Of the two varieties, BARI mung-6 gave the highest seed yield (1.72 t ha-1) next to the higher plant growth and straw yield On the contrary, BARI mung-5 cultivar produced the lowest seed yield (1.57t ha-1), plant growth, and straw yield. The results showed that T3 (20-50-35 NPK kg ha-1) treatment was the best treatment as regards plant growth and yield parameters. The highest seed yield was produced by treated plot T3 (20-50-35 NPK kg ha-1) over the untreated control plot, T1, T2, T4, and T5, respectively. As for the combined effect, V1T3 (BARI mung-6 and T3 = 20kg N + 50kg P + 35kg K ha-1) performed the best results in all growth and development characters. Therefore, the combined application of 20-50-35 kg NPK ha-1 might be considered to be found optimal to get a considerable seed yield of mungbean variety BARI mung-6.


2006 ◽  
Vol 46 (8) ◽  
pp. 1077 ◽  
Author(s):  
B. W. Dunn ◽  
G. D. Batten ◽  
T. S. Dunn ◽  
R. Subasinghe ◽  
R. L. Williams

Straighthead is a ‘physiological’ disorder of rice, the symptoms being floret sterility, deformed florets and panicles and reduced grain yield. Straighthead in rice is difficult to investigate because of its unpredictable occurrence under field conditions. An experiment was conducted in south-eastern Australia in 1996 to investigate the effect of rate and timing of N fertilisation on growth and yield of rice. The presence of straighthead at this location gave a unique opportunity to study the influence of crop N status. This paper reports the influence of N application on straighthead symptoms during this experiment. A significant reduction of straighthead occurred with higher rates of N application. Application of 250 kg N/ha pre-flood, improved plant growth and vigour with subsequent increased uptake and accumulation of S, P, K, Mg, Cu, Mn and Zn in the plant at panicle initiation. The reduction of straighthead at high nitrogen rates may be due to improved uptake of several essential nutrients, and Cu may be a critical nutrient. This study and earlier observations have shown the application of optimal levels of pre-flood nitrogen to achieve grain yields greater than 10 t/ha may reduce straighthead severity in the Australian rice-growing environment. The results in this paper are not presented as recommendations to growers but a contribution to the currently limited literature on straighthead in Australia.


2016 ◽  
Vol 41 (1) ◽  
pp. 163-171 ◽  
Author(s):  
MA Razzaque ◽  
MM Haque ◽  
MA Karim ◽  
ARM Solaiman

A pot culture experiment was conducted at the Bangabandhu Sheikh Mujibur Rahman Agricultural University (BSMRAU), Gazipur during kharif II, 2012 to evaluate the nodulation, biological nitrogen fixation and yield potential of genotypes of mungbean under varying levels of N application. There were 10 mungbean genotypes viz. IPSA 12, GK 27, IPSA 3, IPSA 5, ACC12890055, GK 63, ACC12890053, BU mug 4, BARI Mung 6 and Binamoog 5, each genotype treated with six levels of N (0, 20, 40, 60, 80 and 100 kg N ha-1) . Among the genotypes, the IPSA 12 at 40 kg N ha-1 produced the maximum number of nodules (14.54 plant-1) as well as the highest nitrogen fixation (2.684 mmol C2H4). This resulted in the highest seed yield (14.22 g plant-1). The genotype ACC12890053 recorded the lowest nodulation (6 plant-1), nitrogen fixation (1.134) and seed yield (7.33 g plant-1).Bangladesh J. Agril. Res. 41(1): 163-171, March 2016


ISRN Agronomy ◽  
2012 ◽  
Vol 2012 ◽  
pp. 1-11 ◽  
Author(s):  
Zareen Khan ◽  
Grant Guelich ◽  
Ha Phan ◽  
Regina Redman ◽  
Sharon Doty

Endophytic associations with plants have a beneficial effect for many different plant species with some of them being host specific. Several endophytes isolated from poplar and willow were screened for their effects on commercially important crops including corn, tomato, pepper, squash, sunflower, and grasses. Most of these endophytes produce growth hormones such as indoleacetic acid (IAA) and have the nitrogenase gene required for nitrogen fixation. The effects of these isolates on plant growth and yield were evaluated under greenhouse conditions. We found that inoculated plants not only had better viability and earlier flowering and fruiting, they also had increased plant growth and fruit yields when grown in nitrogen-limited soil. In a particular variety of perennial rye grass, the endophytes increased the total nitrogen content of the plants, indicative of nitrogen fixation, in addition to promoting plant growth. The use of specific endophytes may be preferable to the use of chemical fertilizers because of the monetary and environmental costs, contributing to more sustainable agricultural systems.


2021 ◽  
Vol 11 ◽  
Author(s):  
Vinício Oliosi Favero ◽  
Rita Hilário Carvalho ◽  
Victória Monteiro Motta ◽  
Ana Beatriz Carneiro Leite ◽  
Marcia Reed Rodrigues Coelho ◽  
...  

The mung bean has a great potential under tropical conditions given its high content of grain protein. Additionally, its ability to benefit from biological nitrogen fixation (BNF) through association with native rhizobia inhabiting nodule microbiome provides most of the nitrogen independence on fertilizers. Soil microbial communities which are influenced by biogeographical factors and soil properties, represent a source of rhizobacteria capable of stimulating plant growth. The objective of this study is to support selection of beneficial bacteria that form positive interactions with mung bean plants cultivated in tropical soils, as part of a seed inoculation program for increasing grain yield based on the BNF and other mechanisms. Two mung bean genotypes (Camaleão and Esmeralda) were cultivated in 10 soil samples. Nodule microbiome was characterized by next-generation sequencing using Illumina MiSeq 16S rRNA. More than 99% of nodule sequences showed similarity with Bradyrhizobium genus, the only rhizobial present in nodules in our study. Higher bacterial diversity of soil samples collected in agribusiness areas (MW_MT-I, II or III) was associated with Esmeralda genotype, while an organic agroecosystem soil sample (SE_RJ-V) showed the highest bacterial diversity independent of genotype. Furthermore, OTUs close to Bradyrhizobium elkanii have dominated in all soil samples, except in the sample from the organic agroecosystem, where just B. japonicum was present. Bacterial community of mung bean nodules is mainly influenced by soil pH, K, Ca, and P. Besides a difference on nodule colonization by OTU sequences close to the Pseudomonas genus regarding the two genotypes was detected too. Although representing a small rate, around 0.1% of the total, Pseudomonas OTUs were only retrieved from nodules of Esmeralda genotype, suggesting a different trait regarding specificity between macro- and micro-symbionts. The microbiome analysis will guide the next steps in the development of an inoculant for mung bean aiming to promote plant growth and grain yield, composed either by an efficient Bradyrhizobium strain on its own or co-inoculated with a Pseudomonas strain. Considering the results achieved, the assessment of microbial ecology parameters is a potent coadjuvant capable to accelerate the inoculant development process and to improve the benefits to the crop by soil microorganisms.


Author(s):  
Ram Swaroop Meena ◽  
Dinesh Varma ◽  
Sunil Kumar ◽  
Ekta Kumari

A field experiment was conducted for the management of soil acidity with fertility and lime levels to sustain the productivity of acid soil of Vindhyyan region of Uttar Pradesh (India). Results of the study demonstrated that significant improvement in plant height (49.23 cm), number of nodule (76.95), dry weight of nodule (49.75 mg), highest LAI (4.45), yield parameters, yields, protein (21.39%), nutrient content and total NPK uptake (96.68 kg/ha ) were recorded with 100% RDF. Similar results were observed for all growth and yield parameters such as protein (%), nutrient (%) and total NPK uptake by crop with application of 200 kg lime/ha. A significantly higher seed yield (6.22 q/ha) was recorded with 100 % RDF + 200 kg lime/ha as compared to other treatment combinations, which was at par with 100 % RDF + 300 kg lime/ha and 125 % RDF + 300 kg lime/ha.


1988 ◽  
Vol 110 (3) ◽  
pp. 677-679
Author(s):  
U. V. Mahadkar ◽  
C. S. Saraf

Grain yield of blackgram or urdbean, a popular and highly proteinaceous pulse crop of India, is very low. This is possibly because of its cultivation on marginal and submarginal lands of low soil fertility where little attention is paid to adequate fertilizer, especially phosphorus and nitrogen. Although large achievements have undoubtedly been made in plant nutrition in cereals like wheat and rice, much less attention has been devoted to the development of suitable agro-techniques for production of pulses, especially blackgram. Inoculation with efficient Rhizobium strains and fertility management are important in the production technology of this crop. Though blackgram is regarded as excellent in symbiotic nitrogen fixation, many workers (Pate & Dart, 1961; Shanthakumari & Sinha, 1974) have opined that actual nitrogen fixation starts about 3 weeks after sowing and the senescence of nodules after profuse flowering. This results in nitrogen hunger at initial stages of crop growth as well as in the reproductive stage. Beneficial effects of starter nitrogen in blackgram have been reported by some workers (Singh et al. 1975). However, symbiotic nitrogen fixation may not be adequate to satisfy the N need of developing pods. Since no information is available on the effect of top dressing of nitrogen in the reproductive phase, the present investigations were planned to study the effect of Rhizobium. inoculation and phosphorus as well as time and method of N application on growth and yield of blackgram and its residual effect on fodder sorghum.


2020 ◽  
Vol 15 (2) ◽  
pp. 94-101
Author(s):  
Seyed Afshin Mousavi ◽  
AliReza Shokuhfar ◽  
Shahram Lak ◽  
Mani Mojaddam ◽  
Mojtaba Alavifazel

A low amount of organic matter and insufficient irrigation are two main challenges facing successful crop production in arid and semiarid regions. Application of biochar as an organic amendment to soil not only can help increase organic matter in soil, but also may alleviate adverse effects of water deficit on plant growth and yield production. To test this hypothesis, a two-year field experiment was conducted to assess the effect of sugarcane biochar on yield and yield components of cowpea in water-deficient soil. Treatments consisted of two levels of seed treatment with nitroxin, three levels of biochar application (0, 4, and 8 ton/ha), and a threelevel irrigation regime (60, 90, and 120 mm from evaporation pan class A), laid out in a split-factorial design. Results showed that the seed number per plant was significantly higher in cowpea when grown with biochar, possibly due to the relief of water-deficient stress and higher phosphorus and potassium content. Biomass production of cowpea declined under a severe waterdeficit condition (ir3) compared to normal irrigation (ir1) in 2018 and 2019, decreasing by 39% and 42%, respectively. The maximum biomass obtained from application of 8 ton/ha biochar reached 617.43 and 664.92 g/m2 in 2018 and 2019, respectively. Seeds treated with nitroxin exhibited 10% and 8% greater biomass production in 2018 and 2019 as compared with control treatments. Seed yield increased with the addition of biochar to soil under all irrigation regimes; however, the maximum seed yield of 266.46 and 275.36 g/m2 was observed when there was no water-deficient stress condition and application of 8 ton/ha biochar in 2018 and 2019, respectively.


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