Metabolic activity of hypotonically treated mature boar spermatozoa

1997 ◽  
Vol 9 (6) ◽  
pp. 583 ◽  
Author(s):  
A. R. Jones
Keyword(s):  
Oxygen Uptake ◽  
Metabolic Activity ◽  
Phosphate Buffer ◽  
Cytoplasmic Membrane ◽  
Mitochondrial Activity ◽  
Glycolytic Activity ◽  
Boar Sperm ◽  
Uptake Studies ◽  
Boar Spermatozoa

Treatment of washed boar sperm with hypotonic phosphate buffer removed the acrosome, disrupted the cytoplasmic membrane and almost completely separated the heads from the mid piece-tail segment. As assessed by oxygen uptake studies and their ability to oxidize14C-labelled substrates to 14CO2, hypotonically-treated cells exhibit low glycolytic activity yet mitochondrial activity remains high. Both lactate and glycerol 3-phosphate underwent oxidation and these substrates continued to be metabolized by this preparation which had been stored for up to 10 days at 4°C. Such preparations may be of assistance in the investigation of the biochemistry of boar sperm mitochondria.

Oxidative metabolic activity of boar spermatozoa: a system for assessing anti-glycolytic activity of potential inhibitors in vitro

1989 ◽  
Vol 1 (4) ◽  
pp. 357 ◽  
Author(s):  
AR Jones ◽  
LA Chantrill
Keyword(s):  
Metabolic Activity ◽  
Oxidative Metabolism ◽  
Model System ◽  
High Rate ◽  
Chemical Agents ◽  
Glycolytic Activity ◽  
Metabolic Capability ◽  
Potential Inhibitors ◽  
Boar Spermatozoa

The oxidative metabolic capability of mature boar spermatozoa has been determined in vitro. The high rate of oxidation of fructose, glucose, glycerol, glycerol-3-phosphate and lactate to CO2 and the optimization of incubation conditions indicates that these cells could constitute a model system for investigating the anti-glycolytic activity of potential male anti-fertility agents. The effects of several chemical agents on the oxidative metabolism of boar spermatozoa are reported.

Boar variability affects sperm metabolism activity in liquid stored semen at 5°C

2011 ◽  
Vol 14 (1) ◽  
pp. 21-27 ◽  
Author(s):  
A. Dziekońska ◽  
J. Strzeżek
Keyword(s):  
Metabolic Activity ◽  
Membrane Integrity ◽  
Mitochondrial Activity ◽  
Metabolic Efficiency ◽  
Individual Factor ◽  
Spermatozoa Motility ◽  
Semen Storage ◽  
Individual Source ◽  
The Individual ◽  
Boar Spermatozoa

Boar variability affects sperm metabolism activity in liquid stored semen at 5°CMetabolic activity of boar spermatozoa, liquid stored for three days at 5°C, was measured using bioluminescence for ATP content, fluorescent assay (JC fluorochrome) of mitochondrial activity and oxygen consumption. Sperm motility and plasma membrane integrity (PMI) were simultaneously analyzed. Apart from the statistically significant effect (P < 0.001) of semen storage time, the importance of the individual source of the ejaculate for the analyzed parameters of metabolic efficiency of spermatozoa was shown. This phenomenon was manifested in the interaction of the individual source of the ejaculate with spermatozoa motility, integrity of their membranes and metabolic activity with the passing time of semen preservation. Recorded results indicate that the individual factor may have a significant influence on the technological usefulness of boar spermatozoa for liquid storage. Quality analyses conducted on boar semen stored at 5°C may be used for pre-selection of boars producing sperm with an enhanced tolerance to cold shock.

scholarly journals Molecular Mechanisms Involved in the Impairment of Boar Sperm Motility by Peroxynitrite-Induced Nitrosative Stress

2020 ◽  
Vol 21 (4) ◽  
pp. 1208 ◽  
Author(s):  
Rebeca Serrano ◽  
Nicolás Garrido ◽  
Jose A. Céspedes ◽  
Lauro González-Fernández ◽  
Luis J. García-Marín ◽  
...  
Keyword(s):  
Sperm Motility ◽  
Molecular Mechanisms ◽  
Nitrosative Stress ◽  
Mitochondrial Activity ◽  
Dependent Protein Kinase ◽  
Plasma Membrane Fluidity ◽  
Boar Sperm ◽  
Dependent Protein ◽  
Boar Spermatozoa

Excessive levels of reactive nitrogen species (RNS) produce nitrosative stress. Among RNS is peroxynitrite, a highly reactive free radical generated when nitric oxide reacts with superoxide anion. Peroxynitrite effects have been mainly studied in somatic cells, and in spermatozoa the majority of studies are focused in humans. The aim of this study is to investigate the in vitro peroxynitrite effect on boar spermatozoa functions and the molecular mechanisms involved. Spermatozoa were exposed to the donor 3-morpholinosydnonimine (SIN-1) in non-capacitating or capacitating medium, motility was evaluated by CASA, functional parameters by flow cytometry and sperm protein phosphorylation by Western blotting. SIN-1 treatment, that significantly increases peroxynitrite levels in boar spermatozoa, potentiates the capacitating-stimulated phosphorylation of cAMP-dependent protein kinase 1 (PKA) substrates and GSK-3α. SIN-1 induced peroxynitrite does not decrease sperm viability, but significantly reduces sperm motility, progressive motility, velocities and motility coefficients. Concomitantly, peroxynitrite does not affect mitochondrial membrane potential, plasma membrane fluidity, or A23187-induced acrosome reaction. However, peroxynitrite significantly increases sperm lipid peroxidation in both media. In conclusion, peroxynitrite compromises boar sperm motility without affecting mitochondrial activity. Although peroxynitrite potentiates the phosphorylation of pathways leading to sperm motility, it also causes oxidative stress that might explain, at least partially, the motility impairment.

Inhibition of glycolysis in boar spermatozoa by alpha-chlorohydrin phosphate appears to be mediated by phosphatase activity

1995 ◽  
Vol 7 (5) ◽  
pp. 1089 ◽  
Author(s):  
AR Jones ◽  
LM Porter
Keyword(s):  
Phosphatase Activity ◽  
Triosephosphate Isomerase ◽  
Specific Inhibitor ◽  
Glycolytic Activity ◽  
Boar Sperm ◽  
Inhibition Of Glycolysis ◽  
Boar Spermatozoa

(R,S)-alpha-chlorohydrin-1-phosphate, previously shown to have no anti-glycolytic activity on mature boar sperm in vitro, is a substrate for acid and/or neutral phosphatase(s) that are associated with washed sperm. The high phosphatase activity hydrolyses the ester to alpha-chlorohydrin which undergoes oxidation to (S)-3-chlorolactaldehyde, a specific inhibitor of sperm glyceraldehyde-3-phosphate dehydrogenase and triosephosphate isomerase, thereby exhibiting an anti-glycolytic action.

Glycolytic enzyme activity in hypotonically treated boar spermatozoa

1999 ◽  
Vol 11 (8) ◽  
pp. 409 ◽  
Author(s):  
A. R. Jones ◽  
F. Piccolo
Keyword(s):  
Enzyme Activity ◽  
Lactate Dehydrogenase ◽  
Phosphate Buffer ◽  
Cytoplasmic Membrane ◽  
Glycolytic Enzyme ◽  
The Other ◽  
Simple Preparation ◽  
Chemical Inhibitors ◽  
Boar Spermatozoa ◽  
Phosphate Buffered Saline

Treatment of washed boar sperm with hypotonic phosphate buffer disrupted the cytoplasmic membrane and released the soluble contents and phosphofructokinase, but the other glycolytic enzymes and lactate dehydrogenase were retained. Addition of the appropriate substrates and co-factor(s) to preparations of treated cells in phosphate-buffered saline showed that enzyme activity could be re-instated. This simple preparation should be of assistance in the investigation of specific sections of the glycolytic pathway without the use of chemical inhibitors.

scholarly journals Evaluation of a panel of spermatological methods for assessing reprotoxic compounds in multilayer semen plastic bags

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
M. Schulze ◽  
F. Schröter ◽  
M. Jung ◽  
U. Jakop
Keyword(s):  
Membrane Integrity ◽  
Diglycidyl Ether ◽  
Mitochondrial Activity ◽  
Prolonged Storage ◽  
Prolonged Incubation ◽  
Plastic Bags ◽  
Plastic Materials ◽  
Bisphenol A Diglycidyl Ether ◽  
Semen Preservation ◽  
Boar Spermatozoa

AbstractThe increase of fertility performance in sows is one of the biggest achievements in pig production over the last 30 years. Nevertheless, pig farms using artificial insemination (AI) repeatedly experienced in recent year’s fertility problems with dramatic consequences due to toxic compounds from plastic semen bags. In particular, bisphenol A diglycidyl-ether (BADGE) present in multilayer plastic bags can leach into the semen and could affect the functionality of the spermatozoa. Former studies could not find any alterations in spermatozoa based on the exposure to BADGE. The aim of the study was to evaluate effects of BADGE on boar spermatozoa using an extended panel of spermatological methods. In spring 2019, a large drop in farrowing rates from 92.6 ± 2.3% to 63.7 ± 11.1% in four sow farms in Croatia was detected. In migration studies, BADGE could be identified as a causal toxic compound and leached into the extended semen in concentration of 0.37 ± 0.05 mg/L. Detailed spermatological studies showed that significant predictors for effects on spermatozoa were different levels of motility and kinematic data after a prolonged storage time, thermo-resistance test (prolonged incubation time), mitochondrial activity, membrane integrity and fluidity. No serious effects were observed for sperm morphology and DNA fragmentation. These results provide new insights into the development of a new quality assurance concept for a detailed spermatological examination during testing of plastic materials for boar semen preservation. It could be shown that boar spermatozoa are an excellent biosensor to detect potential toxicity and fertility-relevant compounds.

Energy metabolism in parasitic filariae I: oxygen-uptake studies

1987 ◽  
Vol 15 (6) ◽  
pp. 1174-1175
Author(s):  
ALAN B. O'DOWD ◽  
DAVID J. HAYES ◽  
DAVID L. SELWOOD ◽  
JEREMY N. STABLES
Keyword(s):  
Energy Metabolism ◽  
Oxygen Uptake ◽  
Uptake Studies

INFLUENCE OF CARBON DIOXIDE ABSORBENT AND PERCENTAGE ATMOSPHERIC CARBON DIOXIDE ON OXYGEN UPTAKE BY BOAR SPERMATOZOA

1972 ◽  
Vol 52 (1) ◽  
pp. 57-63
Author(s):  
L. M. SANFORD ◽  
G. J. KING
Keyword(s):  
Carbon Dioxide ◽  
Oxygen Uptake ◽  
Incubation Period ◽  
Potassium Hydroxide ◽  
Atmospheric Carbon Dioxide ◽  
Initial Value ◽  
Atmospheric Carbon ◽  
Cell Motion ◽  
Boar Spermatozoa ◽  
Carbon Dioxide Absorbent

Boar spermatozoa were diluted in Ringer’s fructose or sulfate buffer and incubated aerobically for 4 hr at 37 C (a) in the presence of potassium hydroxide (KOH), hyamine hydroxide, or diethanolamine (DEA) absorbents, and (b) in 1, 2, or 3% atmospheric levels of carbon dioxide. Oxygen uptake by boar spermatozoa was enhanced by the presence of DEA compared with KOH and hyamine hydroxide. Incubation with DEA resulted in increased spermatozoan livability, more desirable cell motion, and smaller increases in buffer pH. Oxygen uptake by boar spermatozoa was optimum with 1 or 2% carbon dioxide, depending upon which buffer cells were diluted in. Levels of 2 and 3% carbon dioxide maintained oxygen uptake and livability of boar spermatozoa during later stages of the incubation period equally as well as 1% carbon dioxide. As the level of atmospheric carbon dioxide increased, buffer pH remained closer to the initial value.

scholarly journals Effects of Bicarbonate on the Respiration and Glycolytic Activity of Boar Spermatozoa

1978 ◽  
Vol 31 (4) ◽  
pp. 385 ◽  
Author(s):  
RN Murdoch ◽  
WD Davis
Keyword(s):  
Carbon Dioxide ◽  
Energy Source ◽  
Oxygen Consumption ◽  
Apparent Effect ◽  
Glycolytic Activity ◽  
Low Levels ◽  
Presence And Absence ◽  
Boar Spermatozoa

The metabolism of washed boar spermatozoa was studied in the presence and absence of low levels of bicarbonate (6 mM) and carbon dioxide (2 %). Bicarbonate stimulated the oxygen consumption of the spermatozoa but had no apparent effect on glycolysis. The stimulatory effect of bicarbonate on respiration depended on the presence of a utilizable exogenous energy source such as glucose, fructose, lactate, or pyruvate and no stimulation occurred when no substrate was added or when acetate was used as substrate. The response of the spermatozoa to bicarbonate also depended on the presence of adequate concentrations of potassium (5 mM) and to a lesser extent magnesium (1 mM).

Biochemical appraisal of a milk diluent for semen

1960 ◽  
Vol 54 (2) ◽  
pp. 166-169
Author(s):  
K. Friis Jakobsen ◽  
T. Mann
Keyword(s):  
Lactic Acid ◽  
Oxygen Uptake ◽  
Incubation Period ◽  
Milk Fat ◽  
Acid Production ◽  
Marked Effect ◽  
Lactic Acid Production ◽  
Milk Product ◽  
Bull Semen ◽  
Boar Spermatozoa

1. A study was made of the effects of a milk diluent on bull, ram and boar spermatozoa. Respiration and fructolysis of spermatozoa were used as the two main criteria of sperm activity. The milk diluent was a standardized and commercially available milk product, consisting of sterilized and homogenized milk, supplemented with milk fat.2. The rate of oxygen uptake measured manometrically in the presence of air was increased by the addition of the milk diluent throughout the entire incubation period. Fructose utilization was assessed by the rate of lactic-acid production. The rate of the anaerobic lactic-acid formation was higher in the presence of the milk diluent during the later stages of incubation.3. The effect of the milk diluent on sperm respiration was most striking in experiments with the sperm-rich portion of boar ejaculate obtained by fractionated collection. A somewhat less marked effect was observed with bull semen, and in ram semen the effect was comparatively weak.

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