Boar variability affects sperm metabolism activity in liquid stored semen at 5°C

2011 ◽  
Vol 14 (1) ◽  
pp. 21-27 ◽  
Author(s):  
A. Dziekońska ◽  
J. Strzeżek
Keyword(s):  
Metabolic Activity ◽  
Membrane Integrity ◽  
Mitochondrial Activity ◽  
Metabolic Efficiency ◽  
Individual Factor ◽  
Spermatozoa Motility ◽  
Semen Storage ◽  
Individual Source ◽  
The Individual ◽  
Boar Spermatozoa

Boar variability affects sperm metabolism activity in liquid stored semen at 5°CMetabolic activity of boar spermatozoa, liquid stored for three days at 5°C, was measured using bioluminescence for ATP content, fluorescent assay (JC fluorochrome) of mitochondrial activity and oxygen consumption. Sperm motility and plasma membrane integrity (PMI) were simultaneously analyzed. Apart from the statistically significant effect (P < 0.001) of semen storage time, the importance of the individual source of the ejaculate for the analyzed parameters of metabolic efficiency of spermatozoa was shown. This phenomenon was manifested in the interaction of the individual source of the ejaculate with spermatozoa motility, integrity of their membranes and metabolic activity with the passing time of semen preservation. Recorded results indicate that the individual factor may have a significant influence on the technological usefulness of boar spermatozoa for liquid storage. Quality analyses conducted on boar semen stored at 5°C may be used for pre-selection of boars producing sperm with an enhanced tolerance to cold shock.

Effect of commercial long-term extenders on metabolic activity and membrane integrity of boar spermatozoa stored at 17°C

2013 ◽  
Vol 16 (3) ◽  
pp. 517-525 ◽  
Author(s):  
A. Dziekońska ◽  
L. Fraser ◽  
A. Majewska ◽  
M. Lecewicz ◽  
Ł. Zasiadczyk ◽  
...  
Keyword(s):  
Metabolic Activity ◽  
Storage Time ◽  
Membrane Integrity ◽  
Prolonged Storage ◽  
Atp Content ◽  
Liquid Storage ◽  
Semen Storage ◽  
Boar Semen ◽  
Boar Spermatozoa

Abstract This study was aimed to analyze the metabolic activity and membrane integrity of boar spermatozoa following storage in long-term semen extenders. Boar semen was diluted with AndrohepR EnduraGuardTM (AeG), DILU-Cell (DC), SafeCell PlusTM (SCP) and Vitasem LD (VLD) extenders and stored for 10 days at 17oC. Parameters of the analyzed sperm metabolic activity included total motility (TMOT), progressive motility (PMOT), high mitochondrial membrane potential (MMP) and ATP content, whereas those of the membrane integrity included plasma membrane integrity (PMI) and normal apical ridge (NAR) acrosome. Extender type was a significant (P < 0.05) source of variation in all the analyzed sperm parameters, except for ATP content. Furthermore, the storage time had a significant effect (P < 0.05) on the sperm metabolic activity and membrane integrity during semen storage. In all extenders the metabolic activity and membrane integrity of the stored spermatozoa decreased continuously over time. Among the four analyzed extenders, AeG and SCP showed the best performance in terms of TMOT and PMI on Days 5, 7 and 10 of storage. Marked differences in the proportions of spermatozoa with high MMP were observed between the extenders, particularly on Day 10 of storage. There were not any marked differences in sperm ATP content between the extenders, regardless of the storage time. Furthermore, the percentage of spermatozoa with NAR acrosomes decreased during prolonged storage, being markedly lower in DC-diluted semen compared with semen diluted with either AeG or SCP extender. The results of this study indicated that components of the long-term extenders have different effects on the sperm functionality and prolonged semen longevity by delaying the processes associated with sperm ageing during liquid storage.

scholarly journals Metabolic activity of boar semen stored in different extenders supplemented with ostrich egg yolk lipoproteins

2017 ◽  
Vol 61 (1) ◽  
pp. 127-133 ◽  
Author(s):  
Anna Dziekońska ◽  
Marek Kinder ◽  
Leyland Fraser ◽  
Jerzy Strzeżek ◽  
Władysław Kordan
Keyword(s):  
Oxygen Consumption ◽  
Metabolic Activity ◽  
Storage Temperature ◽  
Membrane Integrity ◽  
Egg Yolk ◽  
Atp Production ◽  
Beneficial Effects ◽  
Semen Storage ◽  
Boar Semen ◽  
Boar Spermatozoa

AbstractIntroduction:The aim of this study was to evaluate the effect of lipoprotein fraction isolated from ostrich egg yolk (LPFo) on the metabolic activity of boar spermatozoa following liquid semen storage in different extenders and temperatures.Material and Methods:Boar ejaculates were extended in Androhep, Beltsville thawing solution (BTS), and Martín-Rillo and Alias (MR-A) without (control) or with the addition of LPFo and stored for three days at either 5°C or 16°C. The analysed sperm parameters included total motility (TMOT), plasma membrane integrity (PMI), mitochondrial membrane potential (MMP), oxygen consumption, and adenosine triphosphate (ATP) production.Results:The sperm metabolic activity seemed to be higher in the LPFo-based extenders following storage for three days, irrespective of the storage temperature. Compared with the LPFo-free extenders, significantly higher (P < 0.05) sperm PMI and MMP were observed in BTS and MR-A extenders supplemented with LPFo during storage for three days at 5°C. Spermatozoa stored in the BTS-LPFo extender exhibited higher (P < 0.05) TMOT and oxygen consumption, whereas higher (P < 0.05) PMI was observed in spermatozoa stored in Androhep-LPFo and MR-A-LPFo for three days at 16°C. No significant differences (P > 0.05) in ATP content were observed between the LPFo-free and LPFo-based extenders during storage.Conclusions:Supplementation of LPFo to semen extenders had varying effects on the metabolic activity of boar spermatozoa stored at different temperatures. It can be suggested that the interactions of various components of the extenders and seminal plasma with LPFo exert beneficial effects on the sperm metabolic activity during liquid storage of boar semen.

scholarly journals Evaluation of a panel of spermatological methods for assessing reprotoxic compounds in multilayer semen plastic bags

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
M. Schulze ◽  
F. Schröter ◽  
M. Jung ◽  
U. Jakop
Keyword(s):  
Membrane Integrity ◽  
Diglycidyl Ether ◽  
Mitochondrial Activity ◽  
Prolonged Storage ◽  
Prolonged Incubation ◽  
Plastic Bags ◽  
Plastic Materials ◽  
Bisphenol A Diglycidyl Ether ◽  
Semen Preservation ◽  
Boar Spermatozoa

AbstractThe increase of fertility performance in sows is one of the biggest achievements in pig production over the last 30 years. Nevertheless, pig farms using artificial insemination (AI) repeatedly experienced in recent year’s fertility problems with dramatic consequences due to toxic compounds from plastic semen bags. In particular, bisphenol A diglycidyl-ether (BADGE) present in multilayer plastic bags can leach into the semen and could affect the functionality of the spermatozoa. Former studies could not find any alterations in spermatozoa based on the exposure to BADGE. The aim of the study was to evaluate effects of BADGE on boar spermatozoa using an extended panel of spermatological methods. In spring 2019, a large drop in farrowing rates from 92.6 ± 2.3% to 63.7 ± 11.1% in four sow farms in Croatia was detected. In migration studies, BADGE could be identified as a causal toxic compound and leached into the extended semen in concentration of 0.37 ± 0.05 mg/L. Detailed spermatological studies showed that significant predictors for effects on spermatozoa were different levels of motility and kinematic data after a prolonged storage time, thermo-resistance test (prolonged incubation time), mitochondrial activity, membrane integrity and fluidity. No serious effects were observed for sperm morphology and DNA fragmentation. These results provide new insights into the development of a new quality assurance concept for a detailed spermatological examination during testing of plastic materials for boar semen preservation. It could be shown that boar spermatozoa are an excellent biosensor to detect potential toxicity and fertility-relevant compounds.

scholarly journals Quercetin and Naringenin Provide Functional and Antioxidant Protection to Stored Boar Semen

Animals ◽  
2020 ◽  
Vol 10 (10) ◽  
pp. 1930
Author(s):  
Eva Tvrdá ◽  
Mégane Debacker ◽  
Michal Ďuračka ◽  
Ján Kováč ◽  
Ondřej Bučko
Keyword(s):  
Mitochondrial Activity ◽  
Ros Generation ◽  
Dna Integrity ◽  
Membrane Stabilization ◽  
New Information ◽  
Semen Storage ◽  
Boar Semen ◽  
Boar Spermatozoa ◽  
The Impact ◽  
Sample Dilution

In this study, we evaluated the impact of 5–50 μM quercetin (QUE) and naringenin (NAR) on extended boar spermatozoa in the BTS (Beltsville Thawing Solution) medium for 72 h. Spermatozoa motion, membrane, acrosome, and DNA integrity were investigated immediately after sample dilution (0 h) as well as after 24 h, 48 h, and 72 h of semen storage. Furthermore, reactive oxygen species (ROS) and superoxide production, as well as the extent of oxidative damage to the sperm proteins and lipids, were assessed to determine the potential of QUE and NAR to prevent a potential loss of sperm vitality due to oxidative stress development. Our results indicate that the most notable parameter influenced by QUE was the mitochondrial activity, which remained significantly higher throughout the experiment (p < 0.001 and p < 0.0001; 10 μM), and which correlated with the most prominent maintenance of sperm motility (p < 0.01, 48 h; p < 0.05, 72 h). A significant membrane stabilization (p < 0.01, 24 h and 48 h; p < 0.0001, 72 h) and prevention of lipid peroxidation (p < 0.05, 24 h and 48 h; p < 0.01, 72 h) was primarily observed following administration of 10 and 25 μM NAR; respectively. Administration of 10 μM QUE led to a significant decrease of superoxide (p < 0.0001, 48 h and 72 h) while the most notable decline of ROS generation was recorded in the case of 10 and 25 μM NAR (p < 0.001). This study may provide new information on the specific mechanisms of action involved in the favorable effects of natural biomolecules on spermatozoa.

Metabolic activity of hypotonically treated mature boar spermatozoa

1997 ◽  
Vol 9 (6) ◽  
pp. 583 ◽  
Author(s):  
A. R. Jones
Keyword(s):  
Oxygen Uptake ◽  
Metabolic Activity ◽  
Phosphate Buffer ◽  
Cytoplasmic Membrane ◽  
Mitochondrial Activity ◽  
Glycolytic Activity ◽  
Boar Sperm ◽  
Uptake Studies ◽  
Boar Spermatozoa

Treatment of washed boar sperm with hypotonic phosphate buffer removed the acrosome, disrupted the cytoplasmic membrane and almost completely separated the heads from the mid piece-tail segment. As assessed by oxygen uptake studies and their ability to oxidize14C-labelled substrates to 14CO2, hypotonically-treated cells exhibit low glycolytic activity yet mitochondrial activity remains high. Both lactate and glycerol 3-phosphate underwent oxidation and these substrates continued to be metabolized by this preparation which had been stored for up to 10 days at 4°C. Such preparations may be of assistance in the investigation of the biochemistry of boar sperm mitochondria.

scholarly journals Freeze-thawing impairs the motility, plasma membrane integrity and mitochondria function of boar spermatozoa through generating excessive ROS

2021 ◽  
Vol 17 (1) ◽  
Author(s):  
Bin Zhang ◽  
Yan Wang ◽  
Caihong Wu ◽  
Shulei Qiu ◽  
Xiaolan Chen ◽  
...  
Keyword(s):  
Plasma Membrane ◽  
Chromatin Structure ◽  
Membrane Integrity ◽  
Sperm Chromatin ◽  
Mitochondrial Activity ◽  
Atp Content ◽  
Plasma Membrane Integrity ◽  
Freeze Thaw ◽  
Boar Spermatozoa

Abstract Background Cryopreservation is an efficient way to store spermatozoa and is closely associated with the quality of sperm after the freeze-thaw process. During freeze-thaw cycling, excessive reactive oxygen species (ROS) are produced, and the effects of ROS on boar sperm during cryopreservation have not been identified. Results In this study, we evaluated the quality of boar spermatozoa in different steps of cryopreservation (extension, cooling, and thawing for 30 min and 240 min) with or without boar-sperm antioxidant (N-acetylcysteine (NAC)). The ROS levels, sperm motility, plasma membrane integrity, mitochondrial activity, sperm chromatin structure, ATP content, and sperm apoptosis were assayed. After thawing, the ROS level and sperm apoptosis were significantly increased, and the sperm motility, plasma membrane integrity, mitochondrial activity, sperm chromatin structure, and ATP content were significantly impaired compared with those at the extension period and cooling period. Moreover, the addition of N-acetyl L-cysteine (NAC) reversed these changes. Conclusion The freeze-thawing of boar spermatozoa impaired their motility, plasma membrane, mitochondrial activity, sperm chromatin structure and apoptosis by producing excessive ROS. Thus, the downregulation of ROS level by antioxidants, especially the NAC, is important for manufacturing frozen pig sperm to increase reproductive cells and livestock propagation, as well as to improve the application of frozen semen in pigs worldwide.

Cytotoxicity and Immunotoxicity Assessment of NiO Nanoparticles Using the Chlamydomonas reinhardtii

2012 ◽  
Vol 198-199 ◽  
pp. 52-55
Author(s):  
Zhao Xiang Han ◽  
Dan Dan Wu ◽  
Zhen Zhu ◽  
Yu Rong Liu
Keyword(s):  
Chlamydomonas Reinhardtii ◽  
Metabolic Activity ◽  
Membrane Integrity ◽  
Aquatic Organisms ◽  
Lysozyme Activity ◽  
Control Group ◽  
Mitochondrial Activity ◽  
Nio Nanoparticles ◽  
Mitochondrial Depolarization ◽  
Cellular Metabolic Activity

Little is known about the potential behavior and ecotoxicity of nanoparticles to aquatic organisms. The cytotoxicity and immunotoxicity of NiO-Nanoparticles and Chlamydomonas reinhardtii. It shown that the mitochondrial activity was gradually reduced significantly at the different exposure concentrations for NiO nanoparticles. NiO nanoparticles had a similar effect on membrane integrity as that observed for inhibition of cellular metabolic activity, with a significant reduction in membrane integrity. The NiO nanoparticles exhibited a significantly higher degree of BOD compared to the control group. NiO nanoparticles induced increases in MDA levels with incubation time. A significantly higher percent of cells with mitochondrial depolarization was observed when cells were exposed NiO nanoparticles. NiO nanoparticles significantly elevated ROS levels more than four fold at the highest concentration and the lysozyme activity was significantly increase at all the exposure concentrations and incubation times.

scholarly journals In VitroAnalyses of the Effects of Heparin and Parabens on Candida albicans Biofilms and Planktonic Cells

2011 ◽  
Vol 56 (1) ◽  
pp. 148-153 ◽  
Author(s):  
Marisa H. Miceli ◽  
Stella M. Bernardo ◽  
T. S. Neil Ku ◽  
Carla Walraven ◽  
Samuel A. Lee
Keyword(s):  
Candida Albicans ◽  
Metabolic Activity ◽  
Biofilm Formation ◽  
High Dose ◽  
Dependent Manner ◽  
Planktonic Cells ◽  
Content Type ◽  
Heparin Sodium ◽  
The Individual

ABSTRACTInfections and thromboses are the most common complications associated with central venous catheters. Suggested strategies for prevention and management of these complications include the use of heparin-coated catheters, heparin locks, and antimicrobial lock therapy. However, the effects of heparin onCandida albicansbiofilms and planktonic cells have not been previously studied. Therefore, we sought to determine thein vitroeffect of a heparin sodium preparation (HP) on biofilms and planktonic cells ofC. albicans. Because HP contains two preservatives, methyl paraben (MP) and propyl paraben (PP), these compounds and heparin sodium without preservatives (Pure-H) were also tested individually. The metabolic activity of the mature biofilm after treatment was assessed using XTT [2,3-bis-(2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxanilide] reduction and microscopy. Pure-H, MP, and PP caused up to 75, 85, and 60% reductions of metabolic activity of the mature preformedC. albicansbiofilms, respectively. Maximal efficacy against the mature biofilm was observed with HP (up to 90%) compared to the individual compounds (P< 0.0001). Pure-H, MP, and PP each inhibitedC. albicansbiofilm formation up to 90%. A complete inhibition of biofilm formation was observed with HP at 5,000 U/ml and higher. When tested against planktonic cells, each compound inhibited growth in a dose-dependent manner. These data indicated that HP, MP, PP, and Pure-H havein vitroantifungal activity againstC. albicansmature biofilms, formation of biofilms, and planktonic cells. Investigation of high-dose heparin-based strategies (e.g., heparin locks) in combination with traditional antifungal agents for the treatment and/or prevention ofC. albicansbiofilms is warranted.

scholarly journals Testing the Role of Emotion Dysregulation as a Predictor of Juvenile Recidivism

2021 ◽  
Vol 11 (1) ◽  
pp. 83-95
Author(s):  
Kalin Z. Salinas ◽  
Amanda Venta
Keyword(s):  
Emotion Regulation ◽  
Juvenile Offenders ◽  
Emotion Dysregulation ◽  
Longitudinal Research ◽  
Future Research ◽  
Individual Factor ◽  
Treatment And Prevention ◽  
One Year ◽  
The Individual

The current study proposed to determine whether adolescent emotion regulation is predictive of the amount and type of crime committed by adolescent juvenile offenders. Despite evidence in the literature linking emotion regulation to behaviour problems and aggression across the lifespan, there is no prior longitudinal research examining the predictive role of emotion regulation on adolescent recidivism, nor data regarding how emotion regulation relates to the occurrence of specific types of crimes. Our primary hypothesis was that poor emotion regulation would positively and significantly predict re-offending among adolescents. We tested our hypothesis within a binary logistic framework utilizing the Pathways to Desistance longitudinal data. Exploratory bivariate analyses were conducted regarding emotion regulation and type of crime in the service of future hypothesis generation. Though the findings did not indicate a statistically significant relation between emotion regulation and reoffending, exploratory findings suggest that some types of crime may be more linked to emotion regulation than others. In sum, the present study aimed to examine a hypothesized relation between emotion regulation and juvenile delinquency by identifying how the individual factor of dysregulated emotion regulation may have played a role. This study’s findings did not provide evidence that emotion regulation was a significant predictor of recidivism over time but did suggest that emotion regulation is related to participation in certain types of crime one year later. Directions for future research that build upon the current study were described. Indeed, identifying emotion regulation as a predictor of adolescent crime has the potential to enhance current crime prevention efforts and clinical treatments for juvenile offenders; this is based on the large amount of treatment literature, which documents that emotion regulation is malleable through treatment and prevention programming.

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