250 ANTI-MÜLLERIAN HORMONE: IMPLICATIONS FOR FOLLICULAR RESERVE, FOLLICULAR FUNCTION, AND FERTILITY IN THE MARE

2016 ◽  
Vol 28 (2) ◽  
pp. 257 ◽  
Author(s):  
B. A. Ball ◽  
A. Claes ◽  
T. E. Curry ◽  
M. H. T. Troedsson ◽  
E. L. Squires ◽  
...  
Keyword(s):  
Follicular Fluid ◽  
Age Groups ◽  
Antral Follicle ◽  
Antral Follicle Count ◽  
Middle Aged ◽  
University Of Kentucky ◽  
Antral Follicles ◽  
Follicular Reserve ◽  
The University ◽  
The Relationship

Anti-Müllerian hormone (AMH) is a granulosa-cell derived glycoprotein which plays an important regulatory role during folliculogenesis. Antral follicle count (AFC) and AMH have been used in several species to assess ovarian reserve, but relatively little is known about these parameters in mares. The objectives of this study were to determine (i) plasma AMH concentrations and AFC in mares of different ages, as well as their repeatability within and across oestrous cycles; (ii) the relationship between plasma AMH concentrations and AFC with regard to age and follicle size; and (iii) endocrine and molecular changes in growing and preovulatory follicles in relation to AFC and peripheral AMH concentrations. In Exp. 1, young (3–8 years), middle-aged (9–18 years), and old (>18 years) mares were examined by transrectal ultrasonography over 2 to 3 oestrous cycles and changes in the number and size of all detectable antral follicles were recorded. Concentrations of AMH were determined by ELISA. In Exp. 2, molecular and endocrine differences in growing and dominant equine follicles were examined in relationship to variation in AFC, peripheral AMH concentrations and mare age. Follicular fluid oestradiol and AMH concentrations were determined by immunoassay, and expression of AMH, AMHR2, ESR1, ESR2, FSHR, IGF1, INHA, INHBA, and CYP19A1 was determined by quantitative RT-PCR. Antral follicle counts were significantly lower in old mares than in young and middle-aged mares, and AMH concentrations were significantly lower in old mares than in middle-aged mares. A positive relationship was detected between AFC and AMH, and this relationship varied by mare age with a strong correlation in older mares (ρ = 0.86; P < 0.0001), a moderate correlation in middle-aged mares (ρ = 0.60; P = 0.01), and no correlation (ρ = 0.40, P < 0.4) in young mares. Circulating AMH concentrations were significantly related to the number of antral follicles between 6 and 20 mm in diameter, and the repeatability of AFC and AMH concentrations was high within and between oestrous cycles. Within growing follicles, expression of AMH, AMHR2, ESR2, and INHA in granulosa cells was significantly associated with AFC and plasma AMH concentrations, whereas expression of ESR1 and FSHR were significantly correlated with AMH concentrations alone. In follicular fluid, AMH concentrations declined and oestradiol concentrations increased in dominant compared to growing follicles. Our findings indicate that the relationship between AMH and AFC varies across age groups, and that variations in AFC and plasma AMH concentrations are associated with molecular differences in the equine follicle, which, in turn, may have an impact on follicular function. Funded by the Albert G. Clay Endowment at the University of Kentucky.

Should fertile women quit drinking alcohol to produce better quality oocytes?

Zygote ◽  
2020 ◽  
pp. 1-3
Author(s):  
Burcu Ozbakir ◽  
Pinar Tulay
Keyword(s):  
Alcohol Consumption ◽  
Young Women ◽  
Antral Follicle ◽  
Antral Follicle Count ◽  
Control Group ◽  
Social Drinkers ◽  
Antral Follicles ◽  
Healthy Women ◽  
Significant Difference

Summary Alcohol consumption has long been shown to affect both fetal health and pregnancy. In this study, antral follicle count, maturation level of oocytes including morphological assessment and number of metaphase I (MI), metaphase II (MII) and germinal vesicle (GV) stage oocytes obtained from young women (age < 30 years old) with or without alcohol consumption were investigated. In total, 20 healthy women who were social drinkers and 36 healthy women who do not consume alcohol were involved in this study. Women in both study and control groups were undergoing controlled ovarian stimulation. The antral follicle count and the number and quality of the oocytes retrieved were evaluated and recorded. In total, 635 antral follicles, 1098 follicles and 1014 oocytes with 820 MII, 72 MI and 78 GV stage oocytes were collected from the social drinkers. In the control group, 628 antral follicles, 1136 follicles and 1085 oocytes with 838 MII, 93 MI and 102 GV stage oocytes were evaluated. The results of this study showed that the antral follicle count was very similar in both groups. The number of oocytes and MII stage oocytes was slightly higher in the control group, although it was not a significant difference. This study showed that although the consumption of alcohol may have adverse effects post-implantation, it may not have a solid effect during oogenesis in young women. The results of this study are especially important in clinical settings as some women who are social drinkers undergo in vitro fertilization treatments.

scholarly journals THE RELATIONSHIP BETWEEN THE COTININE LEVEL IN URINE AND VITAMIN D IN THE UNIVERSITY STUDENTS

2020 ◽  
Vol 6 (6) ◽  
pp. 214-219
Author(s):  
Ayse Berivan Bakan ◽  
Naim Uzun ◽  
Senay Karadag Arli ◽  
Metin Yıldız
Keyword(s):  
Vitamin D ◽  
University Students ◽  
Vitamin D Deficiency ◽  
Age Groups ◽  
Cotinine Level ◽  
Screening Design ◽  
Significant Relationships ◽  
Blood And Urine Samples ◽  
The University ◽  
The Relationship

Background: Vitamin D deficiency is today acknowledged as a pandemic. Vitamin D deficiency and insufficiency are associated with many chronic diseases, including common cancers, cardiovascular diseases, metabolic syndrome, and infectious and autoimmune diseases.Objective: This study aims to investigate the relationship between the cotinine level in urine and Vitamin D.Methods: This study employed a descriptive and relational screening design.  It was conducted with 74 smoking university students between January 2019 and March 2020. Data were collected through socio-demographic form and Fagerstrom test for nicotine dependence. Besides, the participating students’ blood and urine samples were taken in a suitable environment.Results: The average age of the participating students was 21.50±2.09. Of all the students, 71.6% were males, 62.2% were exposed to the sun between 12 p.m. and 2 p.m., and the average number of cigarettes smoked daily was 13.52±8.22. The average Vitamin D level in blood was 32.4±15.3 (ng/mL), and the average cotinine level in urine was 1.60 ± .32 (ng/L). No statistically significant relationships were found between the Vitamin D level and the cotinine level (p<0.05).Conclusion: Smoking causes diseases and death in many people, and it is a changeable risk factor.  Nursing practices on the struggle against smoking are effective. No significant relationships were found between the Vitamin D level in blood and cotinine level in urine. Similar studies are recommended to be conducted with larger groups and participants from different age groups.

Value of serum and follicular fluid Sirt1,Sirt2 protein levels in predicting the outcome of assisted reproduction

2020 ◽  
Author(s):  
Lingnv Yao ◽  
Wenqin Lin ◽  
Nan Jiang ◽  
Chuyan Li ◽  
Haifeng Cao ◽  
...  
Keyword(s):  
Assisted Reproduction ◽  
Follicular Fluid ◽  
Ovarian Reserve ◽  
Antral Follicle ◽  
Antral Follicle Count ◽  
Advanced Age ◽  
Control Group ◽  
Age Group ◽  
Protein Levels ◽  
Study Population

Abstract Background: To explore whether serum and follicular fluid Sirt1, Sirt2 can reflect ovarian reserve and predict the outcome of assisted reproduction. Methods: The study population comprised 125 patients , 39 in OPOI（occult form of premature ovarian insufficiency）group, 49 in advanced age group , and 37 in control group. The levels of serum Sirt1, Sirt2 were measured on the 2nd to 5th day of menstruation (bSirt1,bSirt2) and HCG day. Follicular fluid Sirt1 (FFSirt1) and Sirt2 (FFSirt2), were determined on OPU (oocyte pick up) day. Results: The level of FFSirt2 in the advanced age group was significantly lower than those in other two groups. FFSirt 2 and Sirt 2 (HCG day ) were negatively correlated with age (r=-0.35, r=-0.19), but there were no value of them for assessing DOR (diminished ovarian reserve). The level of bSirt2 in (cumulative) pregnant group was significantly higher (r=0.24, P=0.00). Conclusions: This was the first study to show that FFSirt2 and Sirt2 (HCG day) might be negatively correlated with age and antral follicle count (AFC). bSirt2 could predict cumulative pregnancy outcome together with anti-Mullerian hormone (AMH), AFC and age.

Antral follicle population in prepubertal and pubertal heifers

2019 ◽  
Vol 31 (1) ◽  
pp. 10 ◽  
Author(s):  
M. M. Seneda ◽  
F. Morotti ◽  
A. F. Zangirolamo ◽  
N. C. da Silva ◽  
T. K. Sanches ◽  
...  
Keyword(s):  
Antral Follicle ◽  
Antral Follicle Count ◽  
Young Female ◽  
Reproductive Efficiency ◽  
Young Females ◽  
Antral Follicles ◽  
Female Cattle ◽  
Selection Of

The antral follicle count (AFC) is an important tool in the selection of bovine females destined for biotechnology. However, little is known about AFC in prepubertal and pubertal heifers. Some challenges inherent to the physiology of young females must be considered to achieve efficient rates with different procedures, such as ovum pick-up and IVF. This paper covers some important topics about ovarian physiology related to the population of antral follicles and reproductive efficiency in young female cattle.

138 Relationships Between Antral Follicle Count, Ovarian Volume, Pre-Antral Follicle Number, and Oocyte Quality

2018 ◽  
Vol 30 (1) ◽  
pp. 209
Author(s):  
G. L. Vasconcelos ◽  
R. Maculan ◽  
N. Alves ◽  
A. L. A. P. L. Ribeiro ◽  
A. W. B. Silva ◽  
...  
Keyword(s):  
Ovarian Follicle ◽  
Primordial Follicle ◽  
Antral Follicle ◽  
Oocyte Quality ◽  
Antral Follicle Count ◽  
Primary Follicle ◽  
Ovarian Volume ◽  
Primordial Follicles ◽  
Antral Follicles ◽  
Grade 3

The objective was to evaluate the possible relationships between AFC, ovarian volume, ovarian follicle reserve and oocyte quality in abattoir-derived ovaries (experiment 1) and in cows (experiment 2) submitted to OPU. Antral follicle counts of ≥25, 16 to 24, and ≤ 16 were used to define AFC classes as high (HAFC), intermediate (IAFC), and low (LAFC) in both experiments. In experiment 1, after antral follicles were aspirated, abattoir ovaries (n = 10 per AFC class) were processed by conventional histology and pre-antral follicles were counted within primordial, primary, secondary, and tertiary classes and classified as either healthy or degenerate under regular microscopy (Cushman et al. 1999). In experiment 2, HAFC (n = 42), IAFC (n = 34), and LAFC (n = 29) cows were submitted to OPU and oocytes classified as grades 1, 2, and 3 or degenerate (IETS, 2010). Antral follicles (≥3 mm in diameter) were counted by ultrasonography. Data were analysed by GENMOD and GLM procedures of SAS (SAS Institute Inc., Cary, NC, USA) after transformations, when required. In experiment 1, mean normal primordial follicle number was higher (P < 0.001) in HAFC (137.0 ± 1.6)a compared with IAFC (52.6 ± 1.9)b and LAFC (20.2 ± 5.3)c ovaries. However, the mean number of degenerate primordial follicles was lower (P < 0.001) in low count ovaries (2.4 ± 0.6) compared with HAFC (19.0 ± 4.7) and IAFC (16.4 ± 1.5, P < 0.001). Normal primary follicle number was higher in the HAFC compared with IAFC and LAFC ovarian classes (86.2 ± 7.0a v. 34.6 ± 5.1b and 14.4 ± 3.3c, respectively; P < 0.01). Degenerate primary follicles were higher in the HAFC compared with LAFC ovarian class (16.8 ± 6.5 v. 5.2 ± 2.64; P < 0.05). Normal secondary follicle number was also higher in the HAFC compared to LAFC ovarian classes (25.2 ± 7.67 v. 2.4 ± 0.8; P < 0.05). The number of degenerate secondary follicles differed (P < 0.01) only between the IAFC and the LAFC ovarian classes (0.6 ± 0.4 and 7.2 ± 2.4, respectively), which were similar (P > 0.5) to the HAFC class (3.8 ± 1.0). In experiment 2, grade 1, 2, and 3 oocytes, viable oocytes, and ovarian volume (mm3) were higher (P < 0.001) in HAFC compared with IAFC and LAFC cows (grade 1: 7.9 ± 0.6a, 4.9 ± 0.7b and 3.3 ± 0.7c; grade 2: 4.0 ± 0.4a, 2.8 ± 0.4b and 1.2c; grade 3: 2.1 ± 0.4a, 2.5 ± 0.4a and 1.3 ± 0.5b, respectively; viable oocytes: 16.3 ± 1.1a, 13.1 ± 1.2b, and 8.1 ± 1.3c, respectively; (volumes: 12.6 ± 0.7a, 10.1 ± 0.8b, and 8.1 ± 0.9c, respectively). In conclusion, high AFC is linked to a higher follicular reserve, oocyte quality, and ovarian volume. It is safe to apply AFC in the selection of bovine females without compromising oocyte or pre-antral follicular population qualities.

scholarly journals Profiling of superoxide dismutase isoenzymes in compartments of the developing bovine antral follicles

Reproduction ◽  
2010 ◽  
Vol 139 (5) ◽  
pp. 871-881 ◽  
Author(s):  
Catherine M H Combelles ◽  
Emily A Holick ◽  
Louis J Paolella ◽  
David C Walker ◽  
Qiaqia Wu
Keyword(s):  
Superoxide Dismutase ◽  
Granulosa Cells ◽  
Follicular Fluid ◽  
Follicular Development ◽  
Cumulus Cells ◽  
Antral Follicle ◽  
Supporting Evidence ◽  
Sod Activity ◽  
Antral Follicles ◽  
Sod Isoforms

The antral follicle constitutes a complex and regulated ovarian microenvironment that influences oocyte quality. Oxidative stress is a cellular state that may play a role during folliculogenesis and oogenesis, although direct supporting evidence is currently lacking. We thus evaluated the expression of the three isoforms (SOD1, SOD2, and SOD3) of the enzymatic antioxidant superoxide dismutase in all the cellular (granulosa cells, cumulus cells, and oocytes) and extracellular (follicular fluid) compartments of the follicle. Comparisons were made in bovine ovaries across progressive stages of antral follicular development. Follicular fluid possessed increased amounts of SOD1, SOD2, and SOD3 in small antral follicles when compared with large antral follicles; concomitantly, total SOD activity was highest in follicular fluids from smaller diameter follicles. SOD1, SOD2, and SOD3 proteins were expressed in granulosa cells without any fluctuations in follicle sizes. All three SOD isoforms were present, but were distributed differently in oocytes from small, medium, or large antral follicles. Cumulus cells expressed high levels of SOD3, some SOD2, but no detectable SOD1. Our studies provide a temporal and spatial expression profile of the three SOD isoforms in the different compartments of the developing bovine antral follicles. These results lay the ground for future investigations into the potential regulation and roles of antioxidants during folliculogenesis and oogenesis.

scholarly journals Follicle Structure Influences the Availability of Oxygen to the Oocyte in Antral Follicles

2011 ◽  
Vol 2011 ◽  
pp. 1-9 ◽  
Author(s):  
A. R. Clark ◽  
Y. M. Stokes
Keyword(s):  
Mathematical Model ◽  
Dissolved Oxygen ◽  
Oxygen Concentration ◽  
Oxygen Transport ◽  
Follicular Fluid ◽  
Antral Follicle ◽  
Antral Follicles ◽  
Cumulus Oocyte Complex ◽  
The Mean ◽  
Size And Structure

The ability of an oocyte to successfully mature is highly dependent on intrafollicular conditions, including the size and structure of the follicle. Here we present a mathematical model of oxygen transport in the antral follicle. We relate mean oxygen concentration in follicular fluid of bovine follicles to the concentration in the immediate vicinity of the cumulus-oocyte complex (COC). The model predicts that the oxygen levels within the antral follicle are dependent on the size and structure of the follicle and that the mean level of dissolved oxygen in follicular fluid does not necessarily correspond to that reaching the COC.

scholarly journals Correlation Between Follicle Stimulating Hormone, Anti-Müllerian Hormone and Antral Follicle Count with Different Age Groups in Infertile Women

2021 ◽  
Vol 20 (1) ◽  
pp. 22-27
Author(s):  
Juthi Bhowmik ◽  
Parveen Fatima ◽  
Jesmine Banu ◽  
Farzana Deeba ◽  
Sheuli Chowdhury ◽  
...  
Keyword(s):  
Negative Correlation ◽  
Ovarian Reserve ◽  
Follicle Stimulating Hormone ◽  
Age Groups ◽  
Antral Follicle ◽  
Antral Follicle Count ◽  
Age Group ◽  
Infertile Women ◽  
Group A ◽  
Study Population

Background: Reduced ovarian reserve predicts poor ovarian response and poor suc-- cess rates in infertile women who undergo Assisted Reproductive Technology (ART). Ovarian reserve decreases with age but the rate of decline varies from one woman to another. Follicle Stimulating Hormone (FSH) Anti-Müllerian Hormone (AMH) and antral follicle count (AFC) represent the three most frequently utilized laboratory tests in determining Ovarian Reserve (OR). To determine correlation between FSH, AMH and AFC in infertile female. Materials and methods: It was an observational (Cross sectional) study. This study was done in the Department of Reproductive Endocrinology and Infertility, Bangabandhu Sheikh Mujib Medical University (BSMMU), Dhaka, between July 2018 to June 2019. The study population consisted of all the diagnosed female infertility patients of reproductive age. The women attending the study center during study period having primary or secondary infertility was considered as study population. They were divided in 4 age groups 21-25, 26-30, 31-35 yrs and 36-40 yrs years. Data was collected using a structured questionnaire following physical & lab examination. For D2 FSH level fasting blood was collected on D2/3 of menstrual cycle, serum FSH level was measured by ADVIA Centraur(R) XP immunoassay system. For S. AMH level blood sample was collected on 2nd day of cycle and measured by BECKMAN COULTER machine using Chemiluminescent Immunoassay method. For AFC count TVS was done on D2-5 of cycle using KONTRON medical USG machine. Collected data were classified, edited, coded and entered into the computer for statistical analysis by using SPSS version 23. Results: Out of 74 patients the mean age was found 32.6±5.5 years. Serum FSH, AMH and AFC were significantly associated with different age group. A negative correlation was found between serum FSH and serum AMH in all age group. But strong correlation found in age group 31-35 yrs and in 36-40 years age group. A negative correlation was found between serum FSH and total AFC in age group 26- 30 years, 31-35 years and 36-40 years respectively. A positive correlation was found between serum AMH and total AFC in all age group but most strong in age group 31-35 years. In multivariate logistic regression analysis serum AMH (<1.0 ng/ml) and total AFC (<5 number) were found to be significantly associated with age group >35 years patients. Conclusion: In all age grqoup, FSH, AMH and AFC correlates but it is more pronounced in advanced age that means >35 years age group. Chatt Maa Shi Hosp Med Coll J; Vol.20 (1); January 2021; Page 22-27

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