Guidelines to cytology smear examination.

Background: Pap smear examination has been universally used as an effective screening tool for early detection of cervical carcinoma. The aim of this study was to assess the utility of Cervical Acid Phosphatase staining as an adjunct to routine Pap smear testing to improvethe sensitivity and specificity of routine Pap smear examination for cervical cancer detection. Materials and Methods: Cervical smears were taken from patients attending the gynecology department and a few cervical cancer screening programmes. One set of slides were alcohol fixed and stained with rapid pap stain and another set of slides were fixed in a special fixative and stained with Cervical Acid Phosphatase -Pap stain. The nuclear features of these Cervical Acid Phosphatase stained dysplastic cells was studied on Pap stain to diagnose cervical intraepithelial lesion/ malignancy. Results: Out of 489 cases included in the study 6 cases were diagnosed with intraepithelial lesion/ malignancy. On Cervical Acid Phosphatase -Pap stain 2 of the cases diagnosed as inflammatory smears on pap stain showed Cervical Acid Phosphatase positivity and thus were re evaluated. Mild nuclear atypia was observed in the Cervical Acid Phosphatase positive cells and these cases were diagnosed as Low grade squamous intraepithelial lesion and later biopsy proven to be Cervical intraepithelial Neoplasia I. Therefore Cervical Acid Phosphatase -Pap test was 100% sensitive and specific for cervical cancer detection. Conclusions: With 100% sensitivity Cervical Acid Phosphatase -Pap test satisfies the criteria of an efficient screening test.

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EOSINOPHILIA AS A PREDICTOR MORBIDITY OF SOIL- TRANSMITTED HELMINTHIASES AMONG WIDODAREN PLANTATION WORKERS IN JEMBER

Journal Of Vocational Health Studies ◽

10.20473/jvhs.v3.i2.2019.47-52 ◽

2019 ◽

Vol 3 (2) ◽

pp. 47

Author(s):

Aditya Primadana ◽

Yudha Nurdian ◽

Dini Agustina ◽

Bagus Hermansyah ◽

Yunita Armiyanti

Keyword(s):

Mixed Infection ◽

Ascaris Lumbricoides ◽

Analytical Study ◽

Blood Smear ◽

Infection Intensity ◽

Neglected Tropical Disease ◽

Hookworm Infection ◽

Weak Correlation ◽

Smear Examination ◽

Blood Smear Examination

Background: Helminthiasis is a neglected tropical disease and still remain a health problem in tropical and sub-tropical countries, including Indonesia. According to WHO, approximately 1,5 billion people or 24% of the world’s population, are infected with Soil-Transmitted Helminth (STH). Morbidity is directly related to the intensity of STH infestation. Some of the studies shown a significant correlation between eosinophilia and helminthiasis. Eosinophilia on the blood smear examination may be used as a marker of STH infestation. Purpose: to determine the correlation between Soil-Transmitted Helminthiases and eosinophilia as a predictor of morbidity of STH infestation among Widodaren Plantation Workers in Jember. Methods: This observational analytical study use the Kato-Katz method to determine infestation of STH and its intensity measured by egg count per gram stool (EPG). Blood smear examination stained with Giemsa conduct to determine eosinophil count. Results: Prevalence of STH infestation was 22,7% (15/66). Ascaris lumbricoides infection was 13,6% (9/66) and Hookworm infection was 4,5% (3/66). There is mixed infection between A. lumbricoides and Hookworm, the prevalence was 4,5%% (3/66). All of the infection intensity was light. The prevalence of eosinophilia was 27,2% (18/66). There was a significant correlation between STH infection and eosinophilia (P=0,000) but, there was a very weak correlation between the intensity of STH infection and eosinophilia (r=0,281; p=0,258). Conclusion: The prevalence of STH infection among Widodaren Plantation Workers is 22,7% and predominated by A. lumbriocides. There is a positive correlation between intensity of STH infection and eosinophilia as predictor morbidity of STH infection with very weak power.

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Comparison of Different Diagnostic Method for Mycobacteria Tuberculosis in Suspected Patients

Journal of Nepal Health Research Council ◽

10.3126/jnhrc.v7i2.3012 ◽

1970 ◽

Vol 7 (2) ◽

pp. 84-88

Author(s):

AR Khagi ◽

S Singh ◽

S Subba ◽

A Bajracharya ◽

R Tuladhar ◽

...

Keyword(s):

Comparative Study ◽

Sputum Smear ◽

Study Group ◽

Bacterial Cells ◽

X Ray ◽

Smear Examination ◽

Group A ◽

Chest X Ray ◽

Group B ◽

Sputum Smear Examination

Background: Microbial examination of smear of AFB by Z-N stain is currently the most rapid method for the detection of M. tuberculosis but its sensitivity is low i.e. required at least 10,000 bacterial cells per ml of sputum and also none specific, but auramine staining method has higher sensitivity than that of the Z-N stain but there are chances of false positive. Objective of this study was to find the correlation between chest X-ray, direct sputum smear examination by Ziehl-Neelsen stain, Auramine fluorochrome stain and sputum culture for M. tuberculosis. Methods: During that study period 250 x 3 samples were taken three each from 250 patients and divided into two groups A and B by performing Auramine fluorochrome stain in all samples . In group A, there were 150 fluorochrome stain positive samples. One each from 150 patient for comparative study of direct sputum smear examination by Ziehl-Neelsen stain, , culture on LJ medium and chest X-ray. Similarly in group B, next 100 fluorochrome stain negative specimens one each from 100 patients were taken for the comparative study of direct sputum smear examination by Ziehl-Neelsen stain, culture and chest X-ray. Results: In the study group A (n=150) all the specimens were positive in Auramine fluorochrome stain and all of them show positive in X-ray but only 134 showed positive in Ziehl-Neelsen stain and 136 showed positive in culture. In the study group B (n=100), all the specimens were negative in Auramine fluorochrome stain and all of them show negative in Ziehl-Neelsen stain but 14 of them were positive in culture and 24 were positive in chest X-ray. Conclusions: The diagnosis of PTB could be made by Auramine fluorochrome microscopy and culture. Key words: auramine fluorochrome stain; culture; mycobacterium tuberculosis; x-ray; ziehl-neelsen. DOI: 10.3126/jnhrc.v7i2.3012 Journal of Nepal Health Research Council Vol.7(2) Apr 2009 84-88

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The Level of knowledge regarding pap smear examination among the childbearing age women’s in RW 04 RT 05, Rangkapan Jaya Baru, Pancoran Mas Depok

International Journal of Nursing and Health Services (IJNHS) ◽

10.35654/ijnhs.v2i2.104 ◽

2019 ◽

Vol 2 (2) ◽

pp. 82-89

Author(s):

Hendrawati Hendrawati

Keyword(s):

Data Collection ◽

Study Design ◽

Pap Smear ◽

Descriptive Study ◽

Check List ◽

Childbearing Age ◽

Smear Examination ◽

Childbearing Age Women ◽

Level Of Knowledge

Level of knowledge of women in childbearing age regarding papsmear consider low. An analytic descriptive study design was used to describe the level of knowledge regarding pap smear examination amonng the childbearing age women. Data collection tools was questionnaire in form of check list. The result confirmed that 39 from 75 participant have an excellent level of knowledge,

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Performance of the XN-2000 WPC channel-flagging to differentiate reactive and neoplastic leukocytosis

Clinical Chemistry and Laboratory Medicine (CCLM) ◽

10.1515/cclm-2015-1105 ◽

2016 ◽

Vol 0 (0) ◽

Author(s):

Peter Schuff-Werner ◽

Peter Kohlschein ◽

Aliaksandra Maroz ◽

Joachim Linssen ◽

Katrin Dreißiger ◽

...

Keyword(s):

Cell Differentiation ◽

Clinical Diagnosis ◽

Viral Infections ◽

Digital Microscopy ◽

Excellent Performance ◽

Blood Samples ◽

Smear Examination ◽

Diagnostic Decision ◽

Hematology Analyzer ◽

Immune Phenotyping

AbstractThe distinction between reactive and neoplastic leukocytes, especially atypical lymphocytes suspected to be reactive or neoplastic, is a particular challenge in automated hematological cell differentiation. The aim of the study was to evaluate the performance of the XN analyzer supplemented with the WPC channel for differentiating between reactive and neoplastic leukocytosis.Blood samples of 253 patients with viral infections, lymphoma or leukemia were analyzed by the Sysmex XN-2000 analyzer equipped with the WPC channel. The results were compared to routine leukocyte differentiation using the routine Sysmex XE-2100 analyzer and automated digital microscopy (DM96). The combined information from standard morphology, immune phenotyping and clinical diagnosis served as a reference.The XN WPC channel demonstrated an excellent performance for differentiating neoplastic (AUC=0.933) and reactive leukocytosis (AUC=0.900) as compared to morphological smear examination (AUC=0.949 and AUC=0.968, respectively) or to the differentiation results of our routine hematology analyzer (AUC=0.630 and AUC=0.635, respectively).Our data show that the combined WDF/WPC of the Sysmex XN-Series analyzer is advantageous in the automated differentiation of neoplastic and reactive leukocytosis, thus supporting the correct diagnostic decision in the daily laboratory routine.

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Dark urine, hypotension and blood smear examination

Intensive Care Medicine ◽

10.1007/s00134-018-5392-5 ◽

2018 ◽

Vol 45 (7) ◽

pp. 1015-1016

Author(s):

Renaud Prevel ◽

Marie Kostine ◽

Arnaud Desclaux ◽

Malek Souayed

Keyword(s):

Blood Smear ◽

Smear Examination ◽

Dark Urine ◽

Blood Smear Examination

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