Influence of dietary volatile fatty acids on the fatty-acid composition of lamb triglycerides, With special reference to the effect of propionate on the presence of branched-chain components

This study evaluates the intake and digestion of nutrients, parameters of rumen fermentation and degradation, omasal digesta and milk fatty acid composition, productive performance, and the concentration of serum metabolites in cows fed 600 g kg-1 sugarcane-based diets containing 0 (control), 15, 30, and 45 g kg-1 sunflower oil (SO) on a dry matter (DM) basis. Four rumen-cannulated Holstein x Gyr cows yielding 15±5 kg day-1 with 110±10 days in milk were allocated in a 4 x 4 Latin square design. Data were analyzed using mixed models, and significant differences were declared at P < 0.05. There was no effect of SO on the intake and apparent digestibility of DM, crude protein, neutral detergent fiber (NDF) and nonfibrous carbohydrates, but there was a linear increase in the intake and digestibility of ether extract. Dietary SO levels did not alter the ruminal degradability parameters for DM and NDF, rumen pH and contents of ammonia N, acetate, propionate and volatile fatty acids. Milk fat content and yield were linearly decreased, whereas a linear increase in milk protein content was observed in response to increasing levels of SO, but with no effect on milk yield. Linear reductions in palmitic and ?-linolenic acid contents, a linear increase in trans-10 C18:1 and elaidic acids, and a quadratic effect on vaccenic and rumenic acids were observed in omasal digesta of cows fed increasing levels of SO. Overall, up to 45 g kg-1 SO can be included on DM of chopped sugarcane-based diets without reducing consumption, apparent digestibility and rumen degradability of DM and fiber. Supplementing chopped sugarcane-based diets with 30 to 45 g kg-1 SO (DM basis) promotes milk fat depression due to the inhibition of mammary lipogenesis by specific rumen-derived fatty acid intermediates of the biohydrogenation of unsaturated C18 fatty acids. The inclusion of 15 to 45 g kg-1 SO in chopped sugarcane-based diets improves the nutritional quality of milk fat, with increases in the levels of oleic, vaccenic and rumenic acids, beneficial to human health, and a reduction in the levels of the hypercholesterolemic lauric, myristic and palmitic acids.

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Variation of Branched-Chain Fatty Acids Marks the Normal Physiological Range for Growth in Listeria monocytogenes

Applied and Environmental Microbiology ◽

10.1128/aem.68.6.2809-2813.2002 ◽

2002 ◽

Vol 68 (6) ◽

pp. 2809-2813 ◽

Cited By ~ 30

Author(s):

David S. Nichols ◽

Kirsty A. Presser ◽

June Olley ◽

Tom Ross ◽

Tom A. McMeekin

Keyword(s):

Fatty Acids ◽

Fatty Acid Composition ◽

Fatty Acid ◽

Listeria Monocytogenes ◽

Acid Composition ◽

Physiological Range ◽

Branched Chain Fatty Acids ◽

Branched Chain ◽

High Degree ◽

Chain Fatty Acids

ABSTRACT The fatty acid composition of Listeria monocytogenes Scott A was determined by close-interval sampling over the entire biokinetic temperature range. There was a high degree of variation in the percentage of branched-chain fatty acids at any given temperature. The percentage of branched C17 components increased with growth temperature in a linear manner. However, the percentages of iso-C15:0 (i15:0) and anteiso-C15:0 (a15:0) were well described by third-order and second-order polynomial curves, respectively. There were specific temperature regions where the proportion of branched-chain fatty acids deviated significantly from the trend established over the entire growth range. In the region from 12 to 13°C there were significant deviations in the percentages of both i15:0 and a15:0 together with a suggested deviation in a17:0, resulting in a significant change in the total branched-chain fatty acids. In the 31 to 33°C region the percentage of total branched-chain components exhibited a significant deviation. The observed perturbations in fatty acid composition occurred near the estimated boundaries of the normal physiological range for growth.

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Fatty Acid Composition of Lipids from Sulphuroxidizing and Methylotrophic Bacteria from Thyasirid and Lucinid Bivalves

Journal of the Marine Biological Association of the United Kingdom ◽

10.1017/s0025315400018294 ◽

1995 ◽

Vol 75 (2) ◽

pp. 445-454 ◽

Cited By ~ 19

Author(s):

J. Gregor Fullarton ◽

P. Wood ◽

John R. Sargent

Keyword(s):

Fatty Acids ◽

Fatty Acid Composition ◽

Fatty Acid ◽

Polyunsaturated Fatty Acids ◽

Acid Composition ◽

Similar Pattern ◽

Methylotrophic Bacteria ◽

Free Living ◽

Branched Chain Fatty Acids ◽

Branched Chain

Lipids of sulphur-oxidizing bacteria isolated fromThyasim flexuosahad large amounts of 18:l(n-7) together with lesser amounts of both 16:l(n-7) and 16:0 as their major fatty acids. Smaller amounts of the cyclopropyl fatty acids, cyclic δ9,10 C16 and especially cyclic δll,12 C18 were also present. A similar pattern was found for methylotrophic bacteria isolated fromThyasira, MyrteaandLucinoma, except that 16:l(n-7) and 16:0 were both present in larger amounts than 18:l(n-7) and small amounts of cyclic δ9,10 C16 and lesser amounts of cyclic All,12 C18 were present in all cases. The fatty acids 18:l(n-7), 16:l(n-7), 16:0, cyclic δ9,10 C16, and cyclic δll,12 C18 were all present in varying amounts in several free-living, non-marine, sulphur-oxidizing bacteria analysed, and in one bacterium both cyclic δ9,10 C18 and cyclic δll,12 C20 were abundant. Branched-chain fatty acids and polyunsaturated fatty acids were not detected in any of the sulphur-oxidizing or methylotrophic bacteria analysed. The lipids of the sulphur-oxidizing and methylotrophic bacteria consisted largely of phosphatidylethanolamine, phosphatidylglycerol and cardiolipin. The results are discussed in terms of fatty acids as indicators of nutritional relationships in bacterial-invertebrate symbioses.

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The Fatty Acid Composition of Vernix caseosa

Australian Journal of Chemistry ◽

10.1071/ch9630679 ◽

1963 ◽

Vol 16 (4) ◽

pp. 679 ◽

Cited By ~ 12

Author(s):

DT Downing

Keyword(s):

Fatty Acids ◽

Fatty Acid Composition ◽

Fatty Acid ◽

Acid Composition ◽

Unsaturated Fatty Acids ◽

Saturated Fatty Acids ◽

Vernix Caseosa ◽

Branched Chain ◽

Hydrolysis Of

The fatty acids obtained on hydrolysis of sebum from newly-born babies contained approximately equal amounts of saturated and unsaturated fatty acids and 10% of α-hydroxyacids. Gas chromatographic examination showed that the saturated fatty acids (C12 to C30) contained 78% branched-chain and 22% normal compounds, while the unsaturated acids (C12 to C32) contained 15% branched and 85% normal compounds. The a-hydroxyacids (C10 to C26) contained 79% branched-chain and 21% normal components and had little if any unsaturation. The high proportion of branched-chain acids suggests that these are not likely to be exogenous.

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PSXV-25 Effects of multiple grass-fed and grain-fed beef systems on meat fatty acid composition

Journal of Animal Science ◽

10.1093/jas/skab235.595 ◽

2021 ◽

Vol 99 (Supplement_3) ◽

pp. 324-324

Author(s):

Sarah C Klopatek ◽

Xiang Yang ◽

James W Oltjen ◽

Payam Vahmani

Keyword(s):

Fatty Acids ◽

Fatty Acid Composition ◽

Fatty Acid ◽

Acid Composition ◽

Fatty Acid Profiles ◽

Conjugated Linoleic Acids ◽

Branched Chain Fatty Acids ◽

Short Period ◽

Bioactive Fatty Acids ◽

Branched Chain

Abstract Retail sales of labeled fresh grass-fed beef in the U.S. have grown from $17 million in 2012 to $272 million in 2016. The objective of present study was to investigate the effect of four typical grass-fed and conventional beef systems raised in the western United States on beef fatty-acid composition. Treatments included: 1) steers stocked on pasture then finished in a feedyard (CON), 2) steers grass-fed for 20 months (GF20), 3) steers grass-fed for 20 months with a 45-day grain finish (GR45), and 4) steers grass-fed for 25 months (GF25). Fatty acids were analyzed with GC using a 100 m CPSil 88 capillary column. All statistical analyses were performed using a GLM procedure in R. Saturated fatty acid (SFA) concentrations were significantly different across treatments (P < 0.05) with GR45 having the lowest SFA of 43% and GF25 having the highest SFA at 46%. Cis-monounsaturated fatty acid (c-MUFA) particularly oleic acid, which is known as a heart healthy fatty acids, were highest for CON at 44% and lowest for GF20 at 37% (P < 0.05). Conjugated linoleic acids (CLA) were significantly lower for the grain-fed treatments (0.54 CON and 0.63 GR45; P < 0.05) compared to grass-fed treatments (0.93 GF20 and 0.86 GF25; P < 0.05). The grass-fed treatments were higher in n-3 PUFA (3.67 GF20 and 2.29 GF25) than CON (0.79; P < 0.05), however feeding grain for a short period of time did not decrease n-3 PUFA concentrations to the CON level (GF45 2.51; P < 0.05). In conclusion, our findings show that beef from grain-fed beef management systems is higher in c-MUFA, while grass-fed is significantly higher in bioactive fatty acids including CLA and branched chain fatty acids. Additional studies are needed to determine if fatty acid profiles between grass-fed and grain-fed beef would result in different health outcomes for the consumers.

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Cellular fatty acid composition of isolates from Legionnaires disease

Journal of Clinical Microbiology ◽

10.1128/jcm.6.2.140-143.1977 ◽

1977 ◽

Vol 6 (2) ◽

pp. 140-143 ◽

Cited By ~ 3

Author(s):

C W Moss ◽

R E Weaver ◽

S B Dees ◽

W B Cherry

Keyword(s):

Mass Spectrometry ◽

Fatty Acids ◽

Fatty Acid Composition ◽

Fatty Acid ◽

Acid Composition ◽

Cellular Fatty Acid ◽

Gas Chromatography Mass Spectrometry ◽

Cellular Fatty Acids ◽

Legionnaires Disease ◽

Branched Chain

The cellular fatty acids of four isolates from Legionnaires disease and two antigenically related isolates were identified by gas chromatography, mass spectrometry, and associated techniques. The six isolates had essentially the same fatty acid composition, which was characterized by large amounts (greater than 80%) of branched-chain acids.

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