Effect of 4-Allyl-1-hydroxy-2-methoxybenzene (eugenol) in the expression of genes involved in cellular cycle and apoptotic process in dental pulp fibroblasts

2021 ◽  
pp. 1-7
Author(s):  
Carolina Estefania De Anda-Cuéllar ◽  
Socorro Ruíz-Rodríguez ◽  
Marine Ortiz-Magdaleno ◽  
Diana María Escobar-García ◽  
Amaury Pozos-Guillén
Keyword(s):  
Dental Pulp ◽  
Apoptotic Process ◽  
Expression Of Genes

scholarly journals 22-hydroxycholesterol leads to efficient dopaminergic specification of human Mesenchymal Stem Cells

2018 ◽  
Keyword(s):  
Bone Marrow ◽  
Functional Assessment ◽  
Dental Pulp ◽  
Dopaminergic Neurons ◽  
Drug Testing ◽  
Human Mesenchymal Stem Cells ◽  
Human Mscs ◽  
Treatment Regimes ◽  
Bone Marrow Adipose Tissue ◽  
Expression Of Genes

AbstractIn the current study, we aim to investigate the neurogenic effect of 22 (R)- hydroxycholesterol (22-HC), on hMSCs obtained from bone marrow, adipose tissue and dental pulp. The effect was evaluated on the basis of detailed morphological and morphometric analysis, expression of genes and proteins associated with maturation of neurons (NF, MAP2, TUJ1, TH), channel ion proteins (Kv4.2 & SCN5A), chemical functionality (DAT), synapse forming tendency of neurons (Synaptophysin) and transcription factors (ngn2 & Pitx3), efficiency of generation of dopaminergic neurons and functional assessment. The percentage of non- DA cells generation (Ach, TPH2, S100 and GFAP positive cells) was also evaluated to confirm selective neurogenic potential of 22-HC. Post analysis, it was observed that 22-HC yields higher percentage of functional DA neurons and has differential effect on various tissue- specific primary human MSCs. This study, which is one of its kinds, may help in improvising the approach of cell based treatment regimes and drug testing in pharmaceutical industry for Parkinson’s disease.

scholarly journals Genetic Comparison of Stemness of Human Umbilical Cord and Dental Pulp

2016 ◽  
Vol 2016 ◽  
pp. 1-12 ◽  
Author(s):  
Chung-Min Kang ◽  
Hyunok Kim ◽  
Je Seon Song ◽  
Byung-Jai Choi ◽  
Seong-Oh Kim ◽  
...  
Keyword(s):  
Gene Expression ◽  
Umbilical Cord ◽  
Dental Pulp ◽  
Tooth Development ◽  
Expression Patterns ◽  
Pcr Analysis ◽  
Human Umbilical Cord ◽  
Tissue Samples ◽  
Qrt Pcr ◽  
Expression Of Genes

This study focuses on gene expression patterns and functions in human umbilical cord (UC) and dental pulp (DP) containing mesenchymal stem cells (MSCs). DP tissues were collected from 25 permanent premolars. UC tissue samples were obtained from three newborns. Comparative gene profiles were obtained using cDNA microarray analysis and the expression of tooth development-associated and MSC-related genes was assessed by the quantitative real-time reverse transcription polymerase chain reaction (qRT-PCR). Genes related to cell proliferation, angiogenesis, and immune responses were expressed at higher levels in UC, whereas genes related to growth factor and receptor activity and signal transduction were more highly expressed in DP. Although UC and DP tissues exhibited similar expression of surface markers for MSCs, UC showed higher expression of CD29, CD34, CD44, CD73, CD105, CD146, and CD166. qRT-PCR analysis showed that CD146, CD166, and MYC were expressed 18.3, 8.24, and 1.63 times more highly in UC, whereas the expression of CD34 was 2.15 times higher in DP. Immunohistochemical staining revealed significant differences in the expression of genes (DSPP,DMP1, andCALB1) related to odontogenesis and angiogenesis in DP. DP and UC tissue showed similar gene expression, with the usual MSC markers, while they clearly diverged in their differentiation capacity.

scholarly journals Electrical Stimulation through Conductive Substrate to Enhance Osteo-Differentiation of Human Dental Pulp-Derived Stem Cells

2019 ◽  
Vol 9 (18) ◽  
pp. 3938 ◽  
Author(s):  
Cheng ◽  
Chen ◽  
Kuo ◽  
Yen ◽  
Mao ◽  
...  
Keyword(s):  
Stem Cells ◽  
Electrical Stimulation ◽  
Dental Pulp ◽  
Calcium Deposition ◽  
Mrna Levels ◽  
Alizarin Red ◽  
Expression Of Genes ◽  
Morphogenetic Protein ◽  
Quantification Analysis ◽  
Human Dental Pulp

Human dental pulp-derived stem cells (hDPSCs) are promising cellular sources for bone healing. The acceleration of their differentiation should be beneficial to their clinical application. Therefore, a conductive polypyrrole (PPy)-made electrical stimulation (ES) device was fabricated to provide direct-current electric field (DCEF) treatment, and its effect on osteo-differentiation of hDPSCs was investigated in this study. To determine the optimal treating time, electrical field of 0.33 V/cm was applied to hDPSCs once for 4 h on different days after the osteo-induction. The alizarin red S staining results suggested that ES accelerated the mineralization rates of hDPSCs. The quantification analysis results revealed a nearly threefold enhancement in calcium deposition by ES at day 0, 2, and 4, whereas the promotion effect in later stages was in vain. To determine the ES-mediated signaling pathway, the expression of genes in the bone morphogenetic protein (BMP) family and related receptors were quantified using qPCR. In the early stages of osteo-differentiation, the mRNA levels of BMP2, BMP3, BMP4, and BMP5 were increased significantly in the ES groups, indicating that these genes were involved in the specific signaling routes induced by ES. We are the first using DCEF to improve the osteo-differentiation of hDPSCs, and our results promise the therapeutic applications of hDPSCs on cell-based bone tissue engineering.

609: Effects of Sirna and Phytoestrogen Treatments on the Expression of Genes Regulated through the Androgen Receptor of Lncap Prostate Cancer Cells

2004 ◽  
Vol 171 (4S) ◽  
pp. 162-162
Author(s):  
Paul Thelen ◽  
Michal Grzmil ◽  
Iris E. Eder ◽  
Barbara Spengler ◽  
Peter Burfeind ◽  
...  
Keyword(s):  
Prostate Cancer ◽  
Androgen Receptor ◽  
Cancer Cells ◽  
Prostate Cancer Cells ◽  
Expression Of Genes

Anti-Diabetic Activity of a Mineraloid Isolate, in vitro and in Genetically Diabetic Mice

2011 ◽  
Vol 81 (1) ◽  
pp. 34-42 ◽  
Author(s):  
Joel Deneau ◽  
Taufeeq Ahmed ◽  
Roger Blotsky ◽  
Krzysztof Bojanowski
Keyword(s):  
Dna Microarrays ◽  
Human Fibroblasts ◽  
Diabetic Animal ◽  
In Vitro Tests ◽  
Diabetic Mice ◽  
Fossil Material ◽  
Expression Of Genes ◽  
Enhanced Expression ◽  
Genetically Diabetic Mice

Type II diabetes is a metabolic disease mediated through multiple molecular pathways. Here, we report anti-diabetic effect of a standardized isolate from a fossil material - a mineraloid leonardite - in in vitro tests and in genetically diabetic mice. The mineraloid isolate stimulated mitochondrial metabolism in human fibroblasts and this stimulation correlated with enhanced expression of genes coding for mitochondrial proteins such as ATP synthases and ribosomal protein precursors, as measured by DNA microarrays. In the diabetic animal model, consumption of the Totala isolate resulted in decreased weight gain, blood glucose, and glycated hemoglobin. To our best knowledge, this is the first description ever of a fossil material having anti-diabetic activity in pre-clinical models.

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