scholarly journals Pre-treatment effects against the diclofenac-induced toxicity by the aqueous leaf extract of Madhuca longifolia on female Wistar albino rats for 10 and 15 days

2020 ◽  
pp. 132-143
Author(s):  
Jerine Peter Simon ◽  
Sibi Karthik Saravanan ◽  
Jayashree Sathiyaranayanan ◽  
Radhakrishnan Vidya ◽  
Usha Kumari ◽  
...  
Keyword(s):  
Leaf Extract ◽  
Control Group ◽  
Albino Rats ◽  
Histopathological Analysis ◽  
Group I ◽  
Madhuca Longifolia ◽  
Aqueous Leaf Extract ◽  
Pre Treatment ◽  
Wistar Albino Rats ◽  
Enzyme Markers

Diclofenac is used to treat rheumatism disorders, which are associated with the damages of renal, gastric and hepatic organs. Diclofenac is a pharmaceutical drug that is known to induce toxicity on its overdosage and long-term usage. Madhuca longifolia is known to have antioxidant, anti-inflammatory and anti-ulcer activity. It is an evergreen tree that is reported to have many ethnomedicinal uses. The other properties of Madhuca longifolia include anti-diabetic, analgesic and anti-microbial activities. Our study aims to evaluate the pre-treatment activity against the diclofenac-induced toxicity by the Madhuca longifolia aqueous leaf extract in Wistar albino rats for 10 and 15 days. Rats were divided as Group-I: Normal control, Group-II: Diclofenac on the last two days, Group-III and group-IIIa: Diclofenac + Aqueous Leaf Extract of Madhuca longifolia, Group-IV and group-IVb: Diclofenac + Silymarin, Group -V and group-Va: Aqueous Madhuca longifolia leaf extract. After the sacrifice, the rats were studied for antioxidant assay, renal enzyme markers, liver enzyme markers, and histopathological analysis of the kidney, stomach, intestine, and liver. As a result, we could identify that Madhuca longifolia has reduced the toxic changes in rats caused by diclofenac.

scholarly journals Activity of Tulsi leaves (Ocimum sanctum linn) in protecting gastric ulcer in rats by cold restrain method

2017 ◽  
Vol 6 (10) ◽  
pp. 2343
Author(s):  
Ayesha Vaseem ◽  
Mazher Ali ◽  
Khuteja Afshan
Keyword(s):  
Leaf Extract ◽  
Mucosal Injury ◽  
Gastric Ulcers ◽  
Ocimum Sanctum ◽  
Control Group ◽  
Albino Rats ◽  
Dependent Manner ◽  
Standard Drug ◽  
Aqueous Leaf Extract ◽  
Pre Treatment

Background: Tulsi (Ocimum sanctum L.) common household plant, a potent adaptogen has been used for its medicinal property for many years. It is known in Ayurveda as Incomparable one, Mother of medicine of Nature and The Queen of Herbs. The present study evaluated the stress induced anti-ulcer effect of tulsi leaves in albino rats.Methods: Albino rats were randomly, allocated to different experimental groups and aqueous leaf extract of Tulsi (Ocimum sanctum) is given for 7 days. Gastric ulcers were induced by cold restrain methods and results were compared with control using distilled water and standard drug Ranitidine.Results: The Anti-ulcer effect of aqueous extract of Tulsi was significant in a dose dependent manner with cold restraint model. The percentage of ulcer protection of Tulsi leaf extract at dose 100mg/kg is 68.85% and at dose of 200mg/kg showed 65.66% and the standard drug Ranitidine showed 78.23% ulcer protection which is significant (p <0.05).Conclusions: The study showed that when Tulsi (Ocimum sanctum) is given at dose 100-200mg/kg for 7 days i.e., pre-treatment in rats for anti-ulcer effect, it caused a significant reduction in the ulcer scores and ulcer index when compared with control group, as evidenced by the gross appearance and histological findings of gastric mucosa in rats. There were few signs of mucosal injury and the percentage of damage was less compared to control group. Serosal surface revealed very few dilated blood vessels and petechial hemorrhages and mucosal surface had few ulcers of varying sizes.

Pregabalin Ameliorates Lipopolysaccharide-Induced Pancreatic Inflammation in Aged Rats

2019 ◽  
Vol 19 (8) ◽  
pp. 1141-1147 ◽  
Author(s):  
Ozlem Ozmen ◽  
Senay Topsakal
Keyword(s):  
Caspase 3 ◽  
Pancreatic Tissue ◽  
Control Group ◽  
Albino Rats ◽  
Histopathological Analysis ◽  
Aged Rats ◽  
Tissue Samples ◽  
Glucose Levels ◽  
Amyloid A ◽  
Group I

Objective: The aim of this study was to examine pancreatic pathology and the prophylactic effects of pregabalin in lipopolysaccharide (LPS) induced sepsis model in aged rats. Methods: Twenty-four female, one-year-old, Wistar Albino rats were assigned to three groups; Group I (control), Group II (study group: 5mg/kg LPS intraperitoneal, single dose) and Group III(treatment group: 5mg/kg LPS+30 mg/kg oral pregabalin one hour before LPS). Animals were sacrificed by exsanguination 6 hours after LPS administration. Blood and pancreatic tissue samples were collected for biochemical, pathological, and immunohistochemical analyses. Results: LPS caused increases in serum amylase and lipase level but led to a reduction in glucose levels. Following histopathological analysis, numerous neutrophil leucocyte infiltrations were observed in vessels and pancreatic tissues. Increased caspase-3 expression was observed in both the endocrine and exocrine pancreas in the LPS group. Similarly, IL-6, caspase-3 (Cas-3), inducible nitric oxide synthase (iNOS), granulocyte colony-stimulating factor (G-CSF) and serum amyloid-A (SAA) expressions were increased by LPS. Pregabalin improved biochemical, histopathological, and immunohistochemical findings. Conclusion: This study showed that LPS causes pathological findings in the pancreas, but pregabalin has ameliorative effects in aged rats with sepsis. Cas-3, IL-6, iNOS, G-CSF, and SAA all play pivotal roles in the pathogenesis of LPS-induced pancreatic damage.

scholarly journals Effect of Amomum subulatum seeds against cypermethrin induced haematological changes in wistar albino rats

2019 ◽  
Vol 8 (3) ◽  
pp. 604
Author(s):  
Goutham Sagarkatte Puttanna ◽  
Purushotham K. ◽  
Swarnalatha Nayak ◽  
Eesha B. Rao ◽  
Ravi Mundugaru
Keyword(s):  
Body Weight ◽  
Normal Control ◽  
Protective Role ◽  
Control Group ◽  
Albino Rats ◽  
Test Drug ◽  
Group Iii ◽  
Group I ◽  
Amomum Subulatum ◽  
Wistar Albino Rats

Background: Cypermethrin is a well know agricultural pesticide used in the developing countries. It is associated with significant toxic potential on human health. Hence the present study was aimed to evaluate the protective role of Amomum subulatum against cypermethrin induced haematalogical changes in Wistar albino rats.Methods: The albino rats were divided into five different groups of six rats each. Group I considered as normal control, group II cypermethrin control (25mg/kg body weight p.o.), group III only test drug and group IV and V administered with cypermethrin 25mg/kg body weight along test drug 1.08 and 2.16mg/kg body weight for 28 consecutive days. At the end of 28th day blood was withdrawn and total haematalogical parameters were estimated.Results: In the cypermethrin control there was significant reduction in the WBC, Platelet, MCHC and considerable reduction in the haemoglobulin concentration in comparison to normal control. The test drug administered at both dose levels was significantly reversed the cypermethrin induced changes in haematalogical parameters.Conclusions: Authors can conclude that the Amomum subulatum has potency to reverse the cypermethrin induced haematalogical changes.

Renal-protective Activity of Dried Gel of Aloe barbadensis (Elwa) on Gentamicin- Induced Nephrotoxicity in Albino Rats

2020 ◽  
Vol 06 ◽  
Author(s):  
Aslam M. ◽  
Shabana Rahman ◽  
Kalim Javed ◽  
Fahamiya Nazeem ◽  
Shiffa Mohamed
Keyword(s):  
Blood Urea Nitrogen ◽  
Renal Damage ◽  
Skin Diseases ◽  
Kidney Diseases ◽  
Control Group ◽  
Albino Rats ◽  
Histopathological Analysis ◽  
Aloe Barbadensis ◽  
Urea Nitrogen ◽  
Group I

Introduction:: Elwa (Aloe barbadensis / Aloe vera) also known as Sibr in Unani medicine which is dried juice obtained by the inspissations and used to treat many diseases, especially digestive disorders, arthritis, gastric ulcer, jaundice, amenorrhoea, kidney diseases, diseases of spleen and liver, constipation, nervine disorders, wounds, burns, scalds and other skin diseases. Methods:: The aim of this study was to evaluate the nephroprotective activity of Aloe barbadensis gel (ABG) on gentamicin-induced nephrotoxicity in rats. Wistar rats were divided into four groups of six animals in each. Group I (control); group II, III and IV were administered gentamicin 100 mg/kg/d subcutaneously from day four to eight. The lower (350 mg/kg) and higher doses (1260 mg/kg) of crude ABG were co-administrated orally in group III and group IV from day 1. On 9th day animals were sacrificed and serum was studied for blood urea nitrogen and serum creatinine and kidneys were prepared for histo-pathological analysis. Gentamicin alone treated group showed increased levels of blood urea nitrogen (118.76  0.90) and creatinine (3.42  0.13) were significantly inhibited in groups pretreated with lower [blood urea nitrogen (40.130.56); creatinine (1.640.13)] and higher [blood urea nitrogen (55.79  0.71); creatinine (2.50  0.06)] doses of crude Aloe barbadensis gel. Results:: The histopathological analysis also showed the protective nature of Aloe barbadensis gel in gentamicin-induced renal damage. Discussion:: In conclusion, the biochemical parameters and histopathological results confirmed nephroprotective effect of the crude Aloe barbadensis gel against gentamicin induced renal damage.

scholarly journals Immunomodulatory activity of methanolic leaf extract of Moringa oleifera in Wistar albino rats

2015 ◽  
Vol 26 (6) ◽  
Author(s):  
Joshua Nfambi ◽  
Godfrey S. Bbosa ◽  
Lawrence Fred Sembajwe ◽  
James Gakunga ◽  
Josephine N. Kasolo
Keyword(s):  
Antibody Titer ◽  
Leaf Extract ◽  
Delayed Type Hypersensitivity ◽  
Control Group ◽  
Albino Rats ◽  
Immunomodulatory Activity ◽  
Neutrophil Adhesion ◽  
Standard Methods ◽  
Group I ◽  
The Mean

AbstractGlobally,An experimental laboratory-based study was done following standard methods and procedures. Nine experimental groups (I, II, III, IV, V, VI, VII, VIII, IX) each comprising of six animals were used. Group I received normal saline. Groups II to IX received 200 mg/kg bwt cyclophosphamide at the beginning of the study. Group III received 50 mg/kg bwt of an immunostimulatory drug levamisole. Groups IV to IX were dosed daily for 14 days with extract at doses of 250, 500, and 1000 mg/kg bwt, respectively, using an intragastric tube. Complete blood count (CBC), delayed-type hypersensitivity reaction (DTH), neutrophil adhesion test, and hemagglutination antibody titer were determined using standard methods and procedures. Statistical analysis was performed using GraphPad prism 5.0a Software.There was an increment in WBC, lymphocyte, and neutrophil counts at a dose of 1000 mg/kg bwt similar to the levamisole-positive control group. The neutrophil adhesion was statistically significant (p≤0.05) for treatment groups that received 1000 mg/kg bwt (29.94%) and 500 mg/kg bwt at 17.28%. The mean percentage increment in footpad thickness was highest (26.9%) after 8 h of injection of antigen in the footpad of rats dosed 500 mg/kg bwt and this later reduced to 25.6% after 24 h. There was a dose-dependent increment in the mean hemagglutination antibody titer to sheep red blood cells (SRBC) from 10.73±0.57 HA units/μL for the 250 mg/kg bwt to 26.22±1.70 HA units/μL for the 1000 mg/kg bwt.Methanolic leaf extract of

Effect of Mentha piperita (Labiatae) and Mentha spicata (Labiatae) on iron absorption in rats

2004 ◽  
Vol 20 (6-10) ◽  
pp. 119-122 ◽  
Author(s):  
Mehmet Akdogan ◽  
Fatih Gultekin ◽  
Mustafa Yontem
Keyword(s):  
Serum Iron ◽  
Binding Capacity ◽  
Mentha Piperita ◽  
Control Group ◽  
Albino Rats ◽  
Mentha Spicata ◽  
Group Iii ◽  
Group I ◽  
Wistar Albino Rats ◽  
Iron Binding Capacity

Aim: The effect of Mentha piperita (Labiatae) and Mentha spicata (Labiatae) teas, which contain different phenol compounds, on iron metabolism was studied. These teas grow in different areas of the city of Isparta, Turkey. These herbals were given to the rats in tea. Methods: Forty-eight male Wistar albino rats weighing 200-250 g were used for this study. The rats were divided into four groups of 12 animals: Group I received no herbal tea (control group); Group II received 20 g/L M. piperita tea; Group III received 20 g/L M. spicata tea; Group IV received 40 g/L M. spicata tea. Herbal teas were prepared daily and provided at all times to the rats over 30 days as drinking water. Results: M. piperita tea caused a decrease in serum iron and ferritin levels P <0.05), and caused an increase in unsaturated iron-binding capacity (UIBC) P <0.01). M. spicata tea caused no significant change in serum iron, ferritin levels and UIBC P <0.05). Conclusion: Both herbal teas inhibited Fe absorption. Inhibition caused by M. spicata tea was dose dependent. Therefore, when drinking these teas, their effect should be considered, especially for children and anemic patients.

scholarly journals Effects of Momordica Charantia Seed Extract on Dexamethasone-Induced Biochemical and Histological Abnormalities in Albino Rats

2020 ◽  
Author(s):  
Dasaraju Rajesh ◽  
Muppala Thejaswini ◽  
MV Advaitha
Keyword(s):  
Blood Sugar ◽  
Statistical Significance ◽  
Liver Volume ◽  
Density Lipoprotein ◽  
Momordica Charantia ◽  
Comparable Efficacy ◽  
Control Group ◽  
Albino Rats ◽  
Histopathological Analysis ◽  
Group I

Introduction: Many preclinical studies and randomised trials in humans have documented the antidiabetic properties of bitter melon, Momordica charantia (M. charantia). Aim: To examine the effects of Momordica CharantiaSeed Extract (MCSE) in comparison to Pioglitazone on Dexamethasone-induced biochemical and histological abnormalities in Albino rats. Materials and Methods: An interventional study was conducted from October, 2015 to December, 2015, with 24 adult healthy Albino rats of Wistar strain, which were divided into four groups of six rats each. Group I (diabetic controls) received dexamethasone alone in a dose of 8 mg/kg intraperitoneally for six days to induce metabolic changes. Group II rats received MCSE 2.5g/kg six days before dexamethasone and six days during dexamethasone administration. Group III rats received pioglitazone 75 mg/kg orally six days before dexamethasone and six days during dexamethasone administration. Rats in Group IV did not receive any medication and was considered as normal control. Blood glucose levels and lipid profiles were measured. Liver weight, liver volume, and histopathological analysis were done. Data were analysed using an Independent t-test followed by ANOVA with Scheffe’s Post-Hoc Test. Statistical significance was set at p<0.05. Results: A significant decrease in the Fasting Blood Sugar and Postprandial Blood Sugar levels was observed in the MCSE and pioglitazone-treated groups as compared to the dexamethasone control group (p<0.01). A significant decrease in the total cholesterol and triglycerides and an increase in High Density Lipoprotein (HDL) levels was observed in MCSE and pioglitazone-treated groups as compared to the dexamethasone control group (p<0.01). In the case of dexamethasone-induced diabetic model, both MCSE and pioglitazone significantly reduced hepatomegaly, dyslipidemia, and hyperglycaemia (p<0.01). Conclusion: MCSE has comparable efficacy to pioglitazone in the prevention of dexamethasone-induced hepatomegaly, dyslipidemia, and hyperglycaemia.

Sign in / Sign up

Export Citation Format

Share Document