Comparison of three filter paper-based devices for safety and stability of viral sample collection in poultry

2020 ◽  
pp. 1-7
Author(s):  
Suwarak Wannaratana ◽  
Aunyaratana Thontiravong ◽  
Somsak Pakpinyo
Keyword(s):  
Filter Paper ◽  
Sample Collection ◽  
Safety And Stability

Techniques' Comparison and Report of the North Carolina Experience

PEDIATRICS ◽  
1989 ◽  
Vol 83 (5) ◽  
pp. 843-848
Author(s):  
Thomas R. Kinney ◽  
Martha Sawtschenko ◽  
Mary Whorton ◽  
Jean Shearin ◽  
Christy Stine ◽  
...  
Keyword(s):  
North Carolina ◽  
Filter Paper ◽  
Screening Program ◽  
Medical Center ◽  
Sample Collection ◽  
Capillary Tubes ◽  
Blood Samples ◽  
Screening Programs ◽  
The North ◽  
Duke University

Controversy still exists as to the best laboratory method to use to screen newborns for sickle cell disease and other hemoglobinopathies. The proposed methods include hemoglobin electrophoresis, column chromatography, isoelectric focusing, and high performance liquid chromatography. There is also debate concerning the preferred method of sample collection. The proposed methods of sample collection include cord blood or blood obtained from the infant collected in a tube with anticoagulant or on filter paper. We compared hemoglobin electrophoresis patterns from infant blood samples collected in heparinized capillary tubes and on filter paper. This comparison was performed because hemoglobin electrophoresis of dried blood samples collected on filter paper has been advocated as a practical, reliable, and inexpensive method for mass screening programs, although the limitations of this technique have not been explored fully. We also summarize data from the North Carolina Newborn Hemoglobinopathy Screening Program, which relates to the advantages and limitations of hemoglobin electrophoresis from filter paper blood specimens. MATERIALS AND METHODS Specimens Four sets of specimens were used for this study: (1) specimens collected at Duke University Medical Center to compare hemoglobin electrophoresis patterns of hemolysates from filter paper and heparinized capillary tubes, (2) specimens collected by the North Carolina program for hemoglobinopathy screening, (3) specimens routinely collected at Duke University in heparinized capillary tubes for newborn hemoglobinopathy screening, and (4) samples for retesting to examine the error rate of the state program and to confirm screening results compatible with a hemoglobinopathy. Samples for Direct Comparison Between Filter Paper and Heparinized Specimens

Urine and fecal sample collection on filter paper for ovarian hormone evaluations

1995 ◽  
Vol 37 (4) ◽  
pp. 305-315 ◽  
Author(s):  
S. E. Shideler ◽  
C. J. Munro ◽  
H. K. Johl ◽  
H. W. Taylor ◽  
B. L. Lasley
Keyword(s):  
Filter Paper ◽  
Fecal Sample ◽  
Ovarian Hormone ◽  
Sample Collection

Use of blood specimens collected on filter paper in screening for abnormal hemoglobins.

1976 ◽  
Vol 22 (5) ◽  
pp. 685-687 ◽  
Author(s):  
R M Schmidt ◽  
E M Brosious ◽  
S Holland ◽  
J M Wright ◽  
G R Serjeant
Keyword(s):  
Disease Control ◽  
Cellulose Acetate ◽  
Filter Paper ◽  
Whole Blood ◽  
Sample Collection ◽  
Capillary Tubes ◽  
Cellulose Acetate Electrophoresis ◽  
Blood Samples ◽  
Blood Specimens ◽  
The U.S

Abstract Both cellulose acetate electrophoresis and citrate agar electrophoresis were performed on 834 blood samples collected on filter paper in Jamaica and shipped for testing to the National Hemoglobinopathy Standardization Laboratory at the U.S. National Center for Disease Control. Additionally, 30 blood samples collected locally were stored on filter paper, in microhematocrit capillary tubes, and as whole blood specimens; at selected times the samples were tested for stability to determine the best sample-collection technique for hemoglobin electrophoresis. Results were most nearly accurate when both cellulose acetate electrophoresis and citrate agar testing were used. The methods are easy to perform, but results are unreliable if the blood samples on filter paper are stored at 4 degrees C for longer than two weeks before they are tested.

scholarly journals Plasmodium falciparum Allelic Diversity: A Comparison of DNA Extraction from Isolates Collected on Rapid Diagnostic Tests (Rdts) and Filter Paper

2021 ◽  
Author(s):  
Denise Patricia Mawili-Mboumba ◽  
Marie Louise Tshibola Mbuyi ◽  
Noe Patrick M’bondoukwe ◽  
Marielle Karine Bouyou-Akotet
Keyword(s):  
Plasmodium Falciparum ◽  
Nucleic Acids ◽  
Filter Paper ◽  
Diagnostic Tests ◽  
Allelic Diversity ◽  
Storage Conditions ◽  
Rapid Diagnostic Tests ◽  
Sample Collection ◽  
Filter Papers

Background: To perform molecular epidemiologic studies based on large cohorts, material such as RDTs or filter papers are useful for biological sample collection and extraction of RNA or DNA of good quality. Thus, we aimed to assess the quality of DNA extracted from malaria rapid diagnostic tests (RDTs) stored at various temperatures for the analysis of Plasmodium falciparum genetic diversity. Methods: Febrile patients benefitted from free malaria diagnosis using microscopy in a malaria sentinel site, at the Regional Hospital Estuaire-Melen, in Gabon, in 2015. P. falciparum isolates were collected onto one filter paper and 2 similar RDTs devices (Acon®) per patient. Nucleic acids were extracted with QiAmp Qiagen kit from paper and RDTs and the quality of the DNA was analyzed by msp1 gene amplification. Results: Msp1gene amplification was achieved in nucleic acids extracted from all filter papers and RDTs devices (n = 45, 100%). K1 alleles were detected in 93.3% (n = 42/45) of the samples and Mad20 alleles in 73.3% (n = 33/45). The number and the intensity of K1 and/or Mad20 fragments were comparable according to the sample collection material and the storage conditions (room temperature vs -20°C) of the samples. The size of the fragments indicating allelic diversity was comparable in 80% (n=36) of the samples. Conclusion: These data show that RDTs are a valuable source of DNA for malaria parasite genetic polymorphism analysis. Storage conditions of the devices did not influence the quality of DNA extracted from RDTs device, although some alleles may be missed.

scholarly journals Diagnosis of Rickettsial Diseases Using Samples Dried on Blotting Paper

1999 ◽  
Vol 6 (4) ◽  
pp. 483-488 ◽  
Author(s):  
Florence Fenollar ◽  
Didier Raoult
Keyword(s):  
Developing Countries ◽  
Filter Paper ◽  
Rickettsia Conorii ◽  
Sample Collection ◽  
Serum Samples ◽  
Bartonella Quintana ◽  
Serological Studies ◽  
Blood Specimens ◽  
Finger Stick ◽  
Rickettsial Diseases

ABSTRACT The use of filter paper is an inexpensive and convenient method for collecting, storing, and transporting blood samples for serological studies. In addition, samples occupy little space and can be readily transported without refrigeration. Rickettsial diseases often evolve according to an epidemic mode and are now considered reemerging diseases, especially in developing countries, under conditions where fieldwork could be difficult. The suitability of collecting whole-blood specimens on filter paper discs for rickettsial antibody assay was evaluated. Dried blood specimens from 64 individuals with antibodies toCoxiella burnetii, Bartonella quintana, orRickettsia conorii were tested for rickettsial antibodies by microimmunofluorescence. Although occasional titers were 1 or 2 dilutions lower than those of tested serum samples, no statistically significant differences were observed. Among patients with negative serology, no false positives were found. This study demonstrated that the recovery of antibodies from finger-stick blood dried on filter paper after elution produces results comparable to those obtained by recovering antibodies from serum. Storing paper samples for 1 month at room temperature or at 4°C did not significantly affect the level of antibodies recovered. This report shows the utility of this sample collection method in developing countries where refrigeration is not possible and venipuncture is problematic.

scholarly journals Diagnosis of Enterocytozoon bieneusi by PCR in Stool Samples Eluted from Filter Paper Disks

2000 ◽  
Vol 7 (3) ◽  
pp. 504-506 ◽  
Author(s):  
Silvana Carnevale ◽  
Jorge N. Velásquez ◽  
Jorge H. Labbé ◽  
Agustín Chertcoff ◽  
Marta G. Cabrera ◽  
...  
Keyword(s):  
Filter Paper ◽  
Enterocytozoon Bieneusi ◽  
Sample Collection ◽  
Fresh Feces ◽  
Stool Samples ◽  
Formalin Fixed

ABSTRACT We report a PCR-based assay for the detection ofEnterocytozoon bieneusi. We extracted DNA from feces which had been applied to filter paper disks and evaluated four preserving solutions. Infected specimens were identified by electrophoresis of amplicons from concentrated formalin-fixed samples and unconcentrated fresh feces. Our findings demonstrate that this methodology is effective for sample collection, mailing, and diagnosis of this pathogen.

Effect of sample collection on α-galactosidase A enzyme activity measurements in dried blood spots on filter paper

2009 ◽  
Vol 403 (1-2) ◽  
pp. 159-162 ◽  
Author(s):  
Petra Olivova ◽  
Kristen van der Veen ◽  
Emmaline Cullen ◽  
Michael Rose ◽  
X. Kate Zhang ◽  
...  
Keyword(s):  
Enzyme Activity ◽  
Filter Paper ◽  
Dried Blood Spots ◽  
Sample Collection ◽  
Blood Spots ◽  
Activity Measurements

scholarly journals Standardization of Trypanosoma cruzi DNA extraction and purification protocol from samples collected on Whatman 903 filter paper to Chagas disease diagnosis.

2020 ◽  
Author(s):  
Laura Smeldy Jurado Medina ◽  
Griselda Ballering ◽  
Margarita Bisio ◽  
Rodrigo Ojeda ◽  
Jaime M Altcheh
Keyword(s):  
Trypanosoma Cruzi ◽  
Dna Extraction ◽  
Chagas Disease ◽  
Filter Paper ◽  
Metabolic Diseases ◽  
Vero Cells ◽  
Disease Diagnosis ◽  
Neglected Diseases ◽  
Sample Collection ◽  
Serological Tests

Chagas disease (CD) caused by the parasite Trypanosoma cruzi, belongs to the so-called neglected diseases group. In Argentina about 1,500 children are born with congenital Chagas disease per year. The diagnosis of CD in the newborn relies on the ability to detect parasites in the blood by microscopic observation, as the serological tests are ruled out because of the presence of maternal antibodies. CD treatment is more effective during the acute phase of infection. Early diagnosis and treatment of the disease is thus very important. The Argentinian National Program for early detection of metabolic diseases uses Whatman903 filter paper for blood sampling. This type of sample collection presents many advantages as the use of low blood volumes, minimal biological risk, and easy storage and transportation. The objective of the study was to evaluate the conservation efficiency of blood samples on filter paper in order to access good sensitivity on qPCR results for the detection of T. cruzi. To standardize the procedure, negative samples of blood were infected artificially with serial dilutions of trypomastigotes forms of T. cruzi from the TcVI strain obtained by cell culture in Vero cells. Concentrations between 50000 and 5 parasites/mL were prepared and loaded in filter paper for analysis. DNA extraction was conducted by the QIAamp DNA Mini Kit from QIAGEN. For qPCR, a method based on TaqMan technology was used, with a multiplex reaction for quantification of T. cruzi satellite DNA and an internal amplification control (IAC). The detection limit found from our results was 400 parasites/mL, demonstrating that this method could be a reliable option for the diagnosis of congenital CD by the detection of T. cruzi in blood collected in filter paper.

Effect of sample collection, temperature and time of storage on β-galactosidase and total hexosaminidase activities in dried blood collected on filter paper

2011 ◽  
Vol 49 (8) ◽  
Author(s):  
Cristina D. de Castilhos ◽  
Jamila Mezzalira ◽  
Mariana P.S. Goldim ◽  
Frederico G. Werlang ◽  
Janice C. Coelho
Keyword(s):  
Filter Paper ◽  
Clinical Chemistry ◽  
Sampling Technique ◽  
Lysosomal Storage Diseases ◽  
Dried Blood Spots ◽  
Methodological Error ◽  
Lysosomal Storage ◽  
Sample Collection ◽  
Entire Study ◽  
Different Temperatures

AbstractDried blood spots (DBS) on filter paper is a valuable sampling technique in clinical chemistry, but the stability of enzymes used in the diagnosis of lysosomal storage diseases (LSDs) needs to be evaluated.In a first experiment, blood from 20 subjects was collected using a syringe without additives and distributed into EDTA tubes, heparin tubes, and spotted on filter paper for the comparison of sampling effects. In a second experiment, blood from 30 healthy subjects was spotted on filter paper and analyzed for β-galactosidase and total hexosaminidase activities after storage of the samples at different temperatures for up to 180 days.Initially, we observed that enzyme activities were the same, independent of the collection method. When DBS was stored at 37°C the activity of β-galactosidase dropped to 85% of the initial value after 180 days (p<0.05). At all other temperatures (–20°C, 4°C and 25°C), the results were within the methodological error. Total hexosaminidase activity did not change significantly during the entire study period and at different storage temperatures.The two enzymes investigated in the present study may be stored for up to 17 days (β-galactosidase) or 180 days (total hexosaminidase) until analysis without loss of activity.

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