Biosorption mechanisms of cationic and anionic dyes in a low-cost residue from brewer’s spent grain

2020 ◽  
pp. 1-16 ◽  
Author(s):  
Thiago Peixoto de Araújo ◽  
Fernanda de Oliveira Tavares ◽  
Daniel Tait Vareschini ◽  
Maria Angélica S. D. Barros
Keyword(s):  
Low Cost ◽  
Anionic Dyes ◽  
Brewer’S Spent Grain ◽  
Spent Grain

Brewer’s spent grain as substrate for enzyme and reducing sugar production using Penicillium sp. HC1

2021 ◽  
Author(s):  
Marcela Bernal-Ruiz ◽  
Alejandro Correa-Lozano ◽  
Laura Gomez-Sánchez ◽  
Balkys Quevedo-Hidalgo ◽  
Lilia Carolina Rojas-Pérez ◽  
...  
Keyword(s):  
Ammonium Sulfate ◽  
Reducing Sugars ◽  
Low Cost ◽  
Xylanase Activity ◽  
Reducing Sugar ◽  
Endoglucanase Activity ◽  
Brewer’S Spent Grain ◽  
Penicillium Sp ◽  
Brewing Process ◽  
Spent Grain

Brewer’s spent grain (BSG) is the main solid waste from the brewing process. It is recognized as a valuable resource for biobased industries because of its composition, high availability, and low cost. The objective of this study was to employ BSG as a substrate to produce the enzymes endoglucanase, cellobiohydrolase, β-glucosidase, and xylanase, as well as reducing sugars using Penicillium sp. HC1. For enzyme production, we evaluated BSG submerged fermentation at different concentrations (1%, 3%, and 5%, w/v) and two sources of nitrogen (yeast extract and ammonium sulfate) on different days (6, 10, and 12) in a 100 mL Erlenmeyer flask. The highest enzyme activity was obtained after 10 days. The enzyme extract obtained using 3% BSG (w/v) and 5 g L-1 of ammonium sulfate showed the highest xylanase activity (25013 ± 1075 U L-1). Using BSG 5% (w/v) without nitrogen supplementation, the endoglucanase activity was 909.7±14.2 U L-1 while underthe same conditions but using BSG 3% (w/v), the β-glucosidase and cellobiohydrolase activity was 3268.6 ±229.9 U L-1 and 103.15±8.1 U L-1, respectively. Maximum reducing sugar concentrations using an enzyme dosage of 1000 U g-1 of xylanase were: 2.7 g L-1 xylose, 1.7 g L-1 arabinose, and 3.3 g L-1 glucose after 6 h of hydrolysis. Result s demonstrated it is possible to produce enzymes and reducing sugars using Penicillium sp. HC1 and BSG as substrate and BSG grinding only as pretreatment. 

Microwave Assisted Liquefaction with Crude Glycerol as a Potential Method of Brewer’s Spent Grain Utilization

2016 ◽  
Vol 10 (4) ◽  
pp. 445-450 ◽  
Author(s):  
Aleksander Hejna ◽  
◽  
Paulina Kosmela ◽  
Milena Kopczynska ◽  
Jozef Haponiuk ◽  
...  
Keyword(s):  
Crude Glycerol ◽  
Degradation Products ◽  
Degradation Mechanism ◽  
Low Cost ◽  
Potential Method ◽  
Spectroscopic Studies ◽  
Brewer’S Spent Grain ◽  
Spent Grain ◽  
Liquefaction Process ◽  
Shed Light

Brewer’s spent grain was applied as a low-cost industrial type of lignocellulose biomass in a liquefaction process with crude glycerol. Influence of the reaction time and solvent:biomass ratio on the efficiency of the process, chemical structure and basic properties of obtained biopolyols was analyzed. Spectroscopic studies of the prepared polyols and solid residues shed light on the biomass degradation mechanism through application of microwaves and further reaction of degradation products with solvent particles.

scholarly journals Biosurfactant from endophytic Bacillus pumilus 2A: physicochemical characterization, production and optimization and potential for plant growth promotion

2021 ◽  
Vol 20 (1) ◽  
Author(s):  
Olga Marchut-Mikołajczyk ◽  
Piotr Drożdżyński ◽  
Arkadiusz Polewczyk ◽  
Wojciech Smułek ◽  
Tadeusz Antczak
Keyword(s):  
Phaseolus Vulgaris ◽  
Carbon Source ◽  
Plant Growth ◽  
Taguchi Method ◽  
Beta Vulgaris ◽  
Food Processing ◽  
Bacillus Pumilus ◽  
Brewer’S Spent Grain ◽  
Spent Grain ◽  
By Products

Abstract Background Microbial surfactants called biosurfactants, thanks to their high biodegradability, low toxicity and stability can be used not only in bioremediation and oil processing, but also in the food and cosmetic industries, and even in medicine. However, the high production costs of microbial surfactants and low efficiency limit their large-scale production. This requires optimization of management conditions, including the possibility of using waste as a carbon source, such as food processing by-products. This papers describes the production and characterization of the biosurfactant obtained from the endophytic bacterial strain Bacillus pumilus 2A grown on various by-products of food processing and its potential applications in supporting plant growth. Four different carbon and nitrogen sources, pH, inoculum concentration and temperature were optimized within Taguchi method. Results Optimization of bioprocess within Taguchi method and experimental analysis revealed that the optimal conditions for biosurfactant production were brewer’s spent grain (5% w/v), ammonium nitrate (1% w/v), pH of 6, 5% of inoculum, and temperature at 30 °C, leading to 6.8 g/L of biosurfactant. Based on gas chromatography–mass spectrometry and Fourier transform infrared spectroscopy analysis produced biosurfactant was determined as glycolipid. Obtained biosurfactant has shown high and long term thermostability, surface tension of 47.7 mN/m, oil displacement of 8 cm and the emulsion index of 69.11%. The examined glycolipid, used in a concentration of 0.2% significantly enhanced growth of Phaseolus vulgaris L. (bean), Raphanus L. (radish), Beta vulgaris L. (beetroot). Conclusions The endophytic Bacillus pumilus 2A produce glycolipid biosurfactant with high and long tem thermostability, what makes it useful for many purposes including food processing. The use of brewer’s spent grain as the sole carbon source makes the production of biosurfactants profitable, and from an environmental point of view, it is an environmentally friendly way to remove food processing by products. Glycolipid produced by endophytic Bacillus pumilus 2A significantly improve growth of Phaseolus vulgaris L. (bean), Raphanus L. (radish), Beta vulgaris L. (beetroot). Obtained results provide new insight to the possible use of glycolipids as plant growth promoting agents.

scholarly journals Potential of Brewer’s Spent Grain as a Potential Replacement of Wood in pMDI, UF or MUF Bonded Particleboard

Polymers ◽  
2021 ◽  
Vol 13 (3) ◽  
pp. 319
Author(s):  
Marius Cătălin Barbu ◽  
Zeno Montecuccoli ◽  
Jakob Förg ◽  
Ulrike Barbeck ◽  
Petr Klímek ◽  
...  
Keyword(s):  
Internal Bond ◽  
Mechanical Performance ◽  
Animal Feed ◽  
Urea Formaldehyde ◽  
Modulus Of Rupture ◽  
Brewer’S Spent Grain ◽  
Beer Brewing ◽  
Removal Of Heavy Metals ◽  
Dry Conditions ◽  
Spent Grain

Brewer’s spent grain (BSG) is the richest by-product (85%) of the beer-brewing industry, that can be upcycled in a plentiful of applications, from animal feed, bioethanol production or for removal of heavy metals from wastewater. The aim of this research is to investigate the mechanical, physical and structural properties of particleboard manufactured with a mixture of wood particles and BSG gradually added/replacement in 10%, 30% and 50%, glued with polymeric diisocyanate (pMDI), urea-formaldehyde (UF) and melamine urea-formaldehyde (MUF) adhesives. The density, internal bond, modulus of rupture, modulus of elasticity, screw withdrawal resistance, thickness swelling and water absorption were tested. Furthermore, scanning electron microscopy anaylsis was carried out to analyze the structure of the panels after the internal bond test. Overall, it was shown that the adding of BSG decreases the mechanical performance of particleboard, due to reduction of the bonding between wood and BSG particles. This decrease has been associated with the structural differences proven by SEM inspection. Interaction of particles with the adhesive is different for boards containing BSG compared to those made from wood. Nevertheless, decrease in the mechanical properties was not critical for particleboards produced with 10% BSG which could be potentially classified as a P2 type, this means application in non-load-bearing panel for interior use in dry conditions, with high dimensional stability and stiffness.

scholarly journals Recycling of Brewer’s Spent Grain as a Biosorbent by Nitro-Oxidation for Uranyl Ion Removal from Wastewater

ACS Omega ◽  
2021 ◽  
Author(s):  
Yi Su ◽  
Marco Wenzel ◽  
Silvia Paasch ◽  
Markus Seifert ◽  
Wendelin Böhm ◽  
...  
Keyword(s):  
Uranyl Ion ◽  
Brewer’S Spent Grain ◽  
Ion Removal ◽  
Spent Grain

scholarly journals Anticholinesterase Activities of Different Solvent Extracts of Brewer’s Spent Grain

Foods ◽  
2021 ◽  
Vol 10 (5) ◽  
pp. 930
Author(s):  
Rares I. Birsan ◽  
Peter Wilde ◽  
Keith W. Waldron ◽  
Dilip K. Rai
Keyword(s):  
Ethyl Acetate Fraction ◽  
Therapeutic Approaches ◽  
Total Polyphenol ◽  
Brewer’S Spent Grain ◽  
Dietary Polyphenols ◽  
Synthetic Drugs ◽  
Naturally Occurring ◽  
Diferulic Acid ◽  
Spent Grain ◽  
Butyryl Cholinesterase

Cholinesterases, involved in acetylcholine catabolism in the central and peripheral nervous system, have been strongly linked with neurodegenerative diseases. Current therapeutic approaches using synthetic drugs present several side effects. Hence, there is an increasing research interest in naturally-occurring dietary polyphenols, which are also considered efficacious. Food processing by-products such as brewer’s spent grain (BSG) would be a potential bio-source of polyphenols. In this study, polyphenol-rich BSG extracts using 60% acetone and 0.75% NaOH solutions were generated, which were further subjected to liquid–liquid partitioning using various organic solvents. The water-partitioned fractions of the saponified extracts had the highest total polyphenol content (6.2 ± 2.8mgGAE/g dw) as determined by Folin–Ciocalteu reagent, while the LC-MS/MS showed ethyl acetate fraction with the highest phenolics (2.9 ± 0.3mg/g BSG dw). The best inhibitions of acetyl- (37.9 ± 2.9%) and butyryl- (53.6 ± 7.7%) cholinesterases were shown by the diethyl ether fraction of the saponified extract. This fraction contained the highest sum of quantified phenolics (99 ± 21.2µg/mg of extract), and with significant (p < 0.01) inhibitory contribution of decarboxylated-diferulic acid. Amongst the standards, caffeic acid presented the highest inhibition for both cholinesterases, 25.5 ± 0.2% for acetyl- and 52.3 ± 0.8% for butyryl-cholinesterase, respectively, whilst the blends insignificantly inhibited both cholinesterases. The results showed that polyphenol-rich BSG fractions have potentials as natural anti-cholinesterase agents.

Antioxidant Activity of Peptides Extracted from Brewers’ Spent Grain Peptides

2012 ◽  
Vol 554-556 ◽  
pp. 891-899
Author(s):  
Xu Yan Zong ◽  
Li Li ◽  
Xu Qiao Feng ◽  
Hui Bo Luo ◽  
Jian Zhou ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Nutritive Value ◽  
Superoxide Radical ◽  
Reduced Glutathione ◽  
Protein Hydrolysate ◽  
Low Cost ◽  
Reducing Power ◽  
Gel Permeation Chromatography ◽  
Cosmetic Products ◽  
Spent Grain

Brewer’s spent grain (BSG) protein extracted from BSG was hydrolyzed using Alcalase to produce BSG protein hydrolysate. BSG protein hydrolysate was fractionated by ultrafiltration to obtain brown color BSG peptides. Antioxidant activity of BSG peptides was analyzeded and compared with reduced glutathione (GSH). BSG peptides exhibited 50% of scavenging activities on 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical, hydroxyl radical, and superoxide radical with concentrations less than 0.8 mg/mL, 0.6 mg/mL and 0.6 mg/mL, respectively. The reducing power of BSG peptides was 0.70 at the concentration of 2.00 mg/mL. 86.30% of the total amount of the BSG peptides purified by gel permeation chromatography was below 2000 Da. Because of its antioxidant activity, stability, nutritive value and low cost, BSG peptides exerts a possibility to use in food or cosmetic products.

Preparation of cationic waste paper and its application in poisonous dye removal

2013 ◽  
Vol 67 (11) ◽  
pp. 2560-2567 ◽  
Author(s):  
Fan Yang ◽  
Xiaojie Song ◽  
Lifeng Yan
Keyword(s):  
Low Cost ◽  
Aqueous Suspension ◽  
Order Kinetic ◽  
Acid Orange 7 ◽  
Anionic Dyes ◽  
Experimental Conditions ◽  
Adsorption Models ◽  
Acid Orange ◽  
Initial Ph ◽  
Pseudo Second Order

Cationic paper was prepared by reaction of paper with 2,3-epoxypropyltrimethylammonium chloride in aqueous suspension, and tested as low-cost adsorbent for wastewater treatment. The experimental results revealed that anionic dyes (Acid Orange 7, Acid Red 18, and Acid Blue 92) were adsorbed on the cationic paper nicely. The maximum amount of dye Acid Orange 7 adsorbed on cationic paper was 337.2 mg/g in experimental conditions. The effects of initial dye concentration, temperature, and initial pH of dye solution on adsorption capacity of cationic paper were studied. The pseudo-first-order and pseudo-second-order kinetic models were applied to describe the kinetic data. The Freundlich and Langmuir adsorption models were used to describe adsorption equilibrium. The thermodynamic data indicated that the adsorption process of dye on cationic paper occurred spontaneously.

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