Cytotoxic activity of Ferula persica gum essential oil on murine colon carcinoma (CT26) and Vero cell lines

2020 ◽  
Vol 32 (2) ◽  
pp. 169-177 ◽  
Author(s):  
Neda Hosseinzadeh ◽  
Tahoora Shomali ◽  
Saeid Hosseinzadeh ◽  
Fatemeh Raouf Fard ◽  
Jafar Jalaei ◽  
...  
Keyword(s):  
Essential Oil ◽  
Cytotoxic Activity ◽  
Vero Cell ◽  
Colon Carcinoma ◽  
Cell Lines ◽  
Murine Colon Carcinoma ◽  
Vero Cell Lines ◽  
Murine Colon

scholarly journals Chemical Composition and Cytotoxic and Antibacterial Activities of the Essential Oil of Aloysia citriodora Palau Grown in Morocco

2017 ◽  
Vol 2017 ◽  
pp. 1-10 ◽  
Author(s):  
Moulay Ali Oukerrou ◽  
Mounir Tilaoui ◽  
Hassan Ait Mouse ◽  
Inass Leouifoudi ◽  
Abdeslam Jaafari ◽  
...  
Keyword(s):  
Essential Oil ◽  
Essential Oils ◽  
Cytotoxic Activity ◽  
Vero Cell ◽  
Cell Lines ◽  
Cytotoxic Effect ◽  
Mass Spectrometry Analysis ◽  
Gas Chromatography Mass Spectrometry ◽  
Agar Disc ◽  
Vero Cell Lines

The aim of this work is to investigate the in vitro cytotoxic and antibacterial effects of the essential oils of Aloysia citriodora Palau, harvested in different regions of Morocco. The chemical profile was established using gas chromatography-mass spectrometry analysis. The cytotoxic activity against P815, MCF7, and VERO cell lines as well as the normal human peripheral blood mononuclear cells (PBMCs) was evaluated using the MTT assay. Standard, ATCC, strains of bacteria (Escherichia coli, Staphylococcus aureus, and Pseudomonas aeruginosa) were cultivated in Muller Hinton media. Then, agar disc diffusion, minimum inhibitory concentrations (MICs), and minimal bactericidal concentrations (MBCs) were determined using microdilution method. The essential oils obtained were predominantly composed of β-spathulenol (15.61%), Ar-curcumene (14.15%), trans-caryophyllene oxide (14.14%), and neral (10.02%). The results of the assays showed that the cytotoxic effect of the essential oil of A. citriodora was high on P815 and moderate on MCF7 and on VERO cell lines. However, no cytotoxic effect was observed on PBMCs. On the other hand, essential oils showed a significant antimicrobial activity against both Gram-negative and Gram-positive bacteria. MICs ranged between 2.84 and 8.37 mg/ml. Essential oil of A. citriodora leaves possesses significant antibacterial effect and cytotoxic activity against tumor cell lines.

scholarly journals Autophagic Cell Death Is Induced by Acetone and Ethyl Acetate Extracts fromEupatorium odoratum In Vitro: Effects on MCF-7 and Vero Cell Lines

2012 ◽  
Vol 2012 ◽  
pp. 1-9 ◽  
Author(s):  
Faizah Bt. Harun ◽  
Syed Mohsin Syed Sahil Jamalullail ◽  
Khoo Boon Yin ◽  
Zulkhairi Othman ◽  
Anita Tilwari ◽  
...  
Keyword(s):  
Cell Death ◽  
Ethyl Acetate ◽  
Cytotoxic Activity ◽  
Vero Cell ◽  
Cell Lines ◽  
Autophagic Cell Death ◽  
Biologically Active ◽  
Vero Cell Lines ◽  
Mcf 7 ◽  
Ethyl Acetate Extracts

Eupatorium odoratum (EO)contains many biologically active compounds, the anticancer effects of which are not well documented. This study evaluates the cytotoxic effects and mechanism of action ofEOextracts on MCF-7 and Vero cell lines. Evaluation of the cytotoxic activity using MTT assay, morphological alterations, and apoptosis were carried out. Autophagy was evaluated by LC3-A protein expression. Cytotoxic activity, membrane blebbing and ballooning at 24 hours, replacement by mass vacuolation, and double membrane vesicles mimicking autophagy and cell death were observed in the cancer cells. No apoptosis was observed by DNA fragmentation assay. Overexpression of LC3-A protein indicated autophagic cell death. Cell cycle analysis showed G0 and G2/M arrest. The Vero cells did not show significant cell death at concentrations <100 μg/mL. These results thus suggest that acetone and ethyl acetate extracts ofEOinduce cell death through induction of autophagy and hold potential for development as potential anticancer drugs.

scholarly journals Essential Oil from Caesalpinia peltophoroides Flowers – Chemical Composition and in vitro Cytotoxic Evaluation

2013 ◽  
Vol 8 (5) ◽  
pp. 1934578X1300800 ◽  
Author(s):  
Bianca A. de Carvalho ◽  
Olivia S. Domingos ◽  
Murilo Massoni ◽  
Marcelo H. dos Santos ◽  
Marisa Ionta ◽  
...  
Keyword(s):  
Chemical Composition ◽  
Essential Oil ◽  
Cytotoxic Activity ◽  
Colon Carcinoma ◽  
Cell Lines ◽  
Cancer Cell ◽  
Antineoplastic Agents ◽  
Positive Control ◽  
Melanoma B16f10

The chemical composition of the essential oil obtained from the flowers of Caesalpinia peltophoroides Benth (Leguminosae) was determined using GC/FID and GC/MS analysis. Twenty-seven compounds were identified, including n-dodecanol (14.2%), n-tetradecanol (19.3%), and n-pentadecanol (5.6%), as well as methyl tetradecanoate (7.4%) and methyl hexadecanoate (12.7%) as main constituents. In vitro the cytotoxic activity of the crude essential oil was evaluated against six cancer cell lines - murine melanoma (B16F10-Nex2), human glioblastoma (U87), cervical carcinoma (HeLa), leukemia (HL-60), colon carcinoma (HCT), and melanoma (A2058). The essential oil had significant cytotoxic activity on U87, HCT, and A2058 cell lines. The IC50 values found were lower than those determined for the positive control, cisplatin, suggesting that the oil could be a source of new antineoplastic agents.

scholarly journals CYTOTOXIC ACTIVITIES PROFILE OF PARASITE MANGO (Dendrophthoe pentandra) FROM VARIOUS AREAS IN INDONESIA AGAINST T47D BREAST CANCER CELLS AND NORMAL VERO CELL LINES

2019 ◽  
Vol 4 (1) ◽  
pp. 1
Author(s):  
Roihatul Mutiah
Keyword(s):  
Cytotoxic Activity ◽  
Vero Cell ◽  
Cancer Cells ◽  
Cell Lines ◽  
Ethanol Extract ◽  
Assay Method ◽  
Cytotoxic Activities ◽  
Activity Profile ◽  
Three Samples ◽  
Vero Cell Lines

<p><em>Indonesia has so many kinds of a parasite (mistletoe), one of them which has potential anticancer activity is mango parasite (Dendrophthoe pentandra). This research aimed at determining the cytotoxic activity profile of mango parasite leaves extracts from several locations in Indonesia against T47D and Vero cell lines. Mango parasite leaves obtained from 4 locations in Indonesia: Kediri, Pekalongan, Sumatra, and Kalimantan. Three samples was obtained from 3 different sub-districts in each locations. Cytotoxic activities of samples were performed by MTT assay method. The results of this study showed that there were differences between samples obtained from different locations, especially in cytotoxic activity and selectivity indexes of mango parasite leaves (D. pentandra) ethanol extract. D. pentandra obtained from Kalimantan has the most potential cytotoxic activity and could be potentially developed into a T47D breast anticancer agent. Extracts from the Kalimantan have an IC<sub>50</sub> values of287.39 μg/ml, LC<sub>50 </sub>798.28 μg / ml and selectivity index 2.77, which means it was toxic to cancer cells but safe for Vero cell lines, and its selectively kills cancer cells rather than Vero cell lines. The result of this study could support D. pentandra to be developed as a new potential Fitofarmaka.</em></p>

scholarly journals CYTOTOXIC ACTIVITY EVALUATION OF ERIOCAULON CINEREUM R.BR. ON HELA AND VERO CELL LINES

2019 ◽  
pp. 90-93
Author(s):  
PINUS JUMARYATNO ◽  
ARDE TOGA NUGRAHA ◽  
ADILIA TRI HIDAYATI ◽  
BAIQ RISKY WAHYU LISNASARI ◽  
WIDYANUR MAYA DIAHANDARI ◽  
...  
Keyword(s):  
Cervical Cancer ◽  
Hela Cell ◽  
Ethyl Acetate ◽  
Cytotoxic Activity ◽  
Vero Cell ◽  
Cell Lines ◽  
Assay Method ◽  
Phytochemical Screening ◽  
Ultrasound Assisted Extraction ◽  
Vero Cell Lines

Objective: This study aimed to evaluate the cytotoxic activity of the extracts and fractions of Eriocaulon cinereum against HeLa and Vero cell lines,which represent cervical cancer and normal cells, respectively. In addition, a phytochemical screening was carried out to determine the chemicalconstituents in the extracts and the active fractions.Materials and Methods: The extracts of E. cinereum were obtained by ultrasound-assisted extraction method using n-hexane, ethyl acetate, andmethanol, successively. The active extract was fractionated using vacuum liquid chromatography with dichloromethane followed by ethyl acetate. Thecytotoxic activity was evaluated by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay method and was measured using microplatereader at the wavelength 595 nm. The data were analyzed with PROBIT from SPSS 16 for Windows®. In addition, phytochemical screening wasperformed using standard procedures.Results: The cytotoxic evaluation of the extracts of E. cinereum showed that the ethyl acetate extract was the most active extract against HeLa cell linewith the half maximal inhibitory concentration (IC50) value of 580.07 μg/ml. The dichloromethane and ethyl acetate fractions from the active extractof E. cinereum exhibited cytotoxic activity against HeLa cell with the IC50 values of 466.61 μg/ml and 267.34 μg/ml, respectively. In addition, the ethylacetate fraction showed a low cytotoxic effect against Vero cell line. The phytochemical screening of the ethyl acetate fraction indicated the presenceof terpenoids and alkaloids.Conclusion: This finding revealed the anticancer potential of E. cinereum and warranted further investigation for the discovery of new anticanceragents from natural resources for cervical cancer.

scholarly journals Whole-Genome Sequences of Zika Virus FLR Strains after Passage in Vero or C6/36 Cells

2018 ◽  
Vol 6 (4) ◽  
Author(s):  
Lindsey A. Moser ◽  
Lauren M. Oldfield ◽  
Nadia Fedorova ◽  
Vinita Puri ◽  
Susmita Shrivastava ◽  
...  
Keyword(s):  
Vero Cell ◽  
Cell Lines ◽  
Virus Strain ◽  
Zika Virus ◽  
Whole Genome ◽  
Genome Sequences ◽  
Complete Genomes ◽  
Vero Cell Lines

ABSTRACT We report 26 complete genomes of Zika virus (ZIKV) isolated after passaging the Zika virus strain FLR in mosquito (C6/36) and mammalian (Vero) cell lines. The consensus ZIKV genomes we recovered show greater than 99% nucleotide identify with each other and with the FLR strain used as input.

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