Fish Protein Isolates Recovered from Silver Carp (Hypophthalmichthys molitrix) By-Products Using Alkaline pH Solubilization and Precipitation

2015 ◽  
Vol 25 (3) ◽  
pp. 400-413 ◽  
Author(s):  
Saiyi Zhong ◽  
Shouchun Liu ◽  
Jinxuan Cao ◽  
Suhua Chen ◽  
Weimin Wang ◽  
...  
2019 ◽  
Vol 9 (3) ◽  
pp. 563
Author(s):  
Wen-Juan Zhou ◽  
Fa-Xiang Wang ◽  
Jian Yu ◽  
Xiang-Hong Li ◽  
Yong-Le Liu

The cryoprotective effects of different amounts of protein hydrolysates prepared from by-products of silver carp using Protamex and Alcalase on surimi that were subjected to six freeze-thaw cycles were investigated. Commercial cryoprotectant (8% w/w 1:1 sucrose-sorbitol blend, SuSo) and control (without cryoprotectant) groups were used for comparison. After six freeze-thaw cycles, the lowest actomyosin extractability, Ca2+-ATPase activity and total sulfhydryl content, along with the highest surface hydrophobicity of actomyosin, were observed in the control group (P < 0.05). On the contrary, the group with addition of 2 g of hydrolysate prepared by Protamex hydrolysis (PH-2) displayed the highest actomyosin extractability, Ca2+-ATPase activity and correspondingly, lowest surface hydrophobicity of actomyosin (P < 0.05). Total sulfhydryl content of actomyosin and textural properties of heat-set surimi gels were similar between samples with PH-2 and those with SuSo (P > 0.05). Differences in molecular weight distribution, total and free amino acid compositions between the hydrolysates prepared by Protamex and Alcalase hydrolysis were possible reasons attributing to their variable cryoprotective effects on freeze-thawed surimi. Results from this study clearly support that hydrolysate prepared by Protamex hydrolysis at an appropriate amount could serve as an effective cryoprotectant without increasing the sweetness of surimi products. Furthermore, our findings suggest that the hydrolysates follow a different cryoprotection mechanism compared to SuSo (sucrose-sorbitol blend).


2021 ◽  
Vol 26 (4) ◽  
pp. 2808-2817
Author(s):  
PETRUTA PREDA ◽  
ANA-MARIA ENCIU ◽  
MARIOARA AVRAM ◽  
CRISTIANA TANASE ◽  
MARIA DUDAU ◽  
...  

Collagen is widely used as a biomaterial in the pharmaceutical and cosmetic industries, in the production of hydrogels, wound dressings, bioactive nano/microfibers, controlled drug delivery systems, etc. The collagen isolated from the aquatic source has a higher biological activity and low risk of transmitting genetic diseases. In recent years the sustainable socio-economic and environmental principles promote the full use of natural resources. Thus, fish collagen extracted from fish by-products (skin, scales, bones and fins) can be valorized as a new collagen alternative source. In this work the enzymatic hydrolysis with pepsin of collagen isolation from silver carp (Hypophthalmichthys molitrix) tails fish has been investigated. We successfully isolated type I collagen with 90-95% purity as determined by FTIR, UV-Vis, EDX and SDS-PAGE analyses. The cytotoxicity of obtained collagen was evaluating by MTS assays.


2020 ◽  
Author(s):  
Cu Thien Pham

Abstract VAC systems are an integration of garden (vuon), pond (ao) and animal pen (chuong). VAC farming systems can be classified into different types on the basis of the uses and intensity level of orchard and pond. Fruit trees include citrus, longan, mango and sapodilla, and vegetables include cassava, centella, sweet potato and water spinach. Livestock are poultry and/or pigs. A fish polyculture is practised with species such as silver barb (Barbonymus gonionotus), Indian major carps (mrigal, Cirrhinus cirrhosus, and rohu, Labeo rohita), silver carp (Hypophthalmichthys molitrix), grass carp (Ctenopharyngodon idella), giant gourami (Osphronemus goramy), river catfish (Pangasianodon hypophthalmus), hybrid catfish (Clarias macrocephalus × C. gariepinus), common carp (Cyprinus carpio), kissing gouramy (Helostoma temminckii) and Nile tilapia (Oreochromis niloticus). Pig manure is commonly used as fertilizer for crops while rice by-products are mainly used to feed the livestock. Major inputs for ponds are on-farm resources (crop residue, grass, fallen fruit, rice by-products, human waste, poultry manure, urine and washing from the pig sty) for the low input systems, and off-farm feeds (trash fish, fish meal, golden snail) for medium and high input aquaculture systems. Fish production is below 2 t ha-1 year-1 in the low input and 2-10 t ha-1 year-1 for medium and high input aquaculture.In general, VAC farming in the Mekong Delta is practised in a medium farm with an average pond area ranging from 500 to 1,000 m2, producing fish at less than 1 t yr-1, of which about 250 kg is for family consumption and the surplus, if any, for sale is insignificant. As VAC still has many problems and constraints, an appropriate way to develop VAC farming in the Mekong Delta is to provide farmers with farm component technologies and management knowledge.


2005 ◽  
Vol 40 (2) ◽  
pp. 191-201 ◽  
Author(s):  
Nadia Berday ◽  
Driss Zaoui ◽  
Abdeljaouad Lamrini ◽  
Mustapha Abi

Abstract The effect of silver carp (Hypophthalmichthys molitrix Val.) feeding activity on the plankton communities in a high-rate pond technology system (HRPTS) effluent was investigated over a period of 100 days. The experiment was conducted at the experimental wastewater treatment plant of the Agronomic and Veterinary Medicine Institute (AVI) of Rabat, Morocco, using a HRPTS in a fish pond receiving the plant effluent. The effluent was highly dominated by phytoplankton (99.95%). Silver carp could survive and grow in the fish pond. Production was 37 kg with a very low mortality rate (12%). The high specific intestine weight (7%) and intake rates of biomass and phytoplankton by silver carp (616 g kg-1 of fish day-1 and 1.6 x 1011 cell kg-1 of fish day-1, respectively) demonstrated the importance of the feeding activity of the fish. Zooplankton intake rates were lower (2 x 107 bodies kg-1 of fish day-1). The high intestine index (3 to 4.3 for fish sizes of 14 to 22 cm) and the dominance of phytoplankton in the gut contents (99.95%) confirmed an omnivorous/ phytoplanctivorous diet. Silver carp were efficient in removing plankton from the HRPTS effluent. The net removal yields of biomass were 285 g m-3 day-1 and 322 g kg-1 of fish day-1, 7 x 1010 algal cells kg-1 of fish day-1 and 8.7 x 107 zooplankton bodies kg-1 of fish day-1, with net removal rates of 47, 64 and 62%, respectively. The total suspended solids concentration decreased from 211 in the inflow to 112 mg L-1 in the fish pond.


Aquaculture ◽  
1987 ◽  
Vol 64 (2) ◽  
pp. 111-118 ◽  
Author(s):  
Angelito C. Gonzal ◽  
Emiliano V. Aralar ◽  
Josefina Ma.F. Pavico

Aquaculture ◽  
2021 ◽  
pp. 736715
Author(s):  
Matthew G. Hamilton ◽  
Wagdy Mekkawy ◽  
Benoy K. Barman ◽  
Md. Badrul Alam ◽  
Manjurul Karim ◽  
...  

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