scholarly journals Cryoprotective Effects of Protein Hydrolysates Prepared from By-Products of Silver Carp (Hypophthalmichthys Molitrix) on Freeze-Thawed Surimi

2019 ◽  
Vol 9 (3) ◽  
pp. 563
Author(s):  
Wen-Juan Zhou ◽  
Fa-Xiang Wang ◽  
Jian Yu ◽  
Xiang-Hong Li ◽  
Yong-Le Liu
Keyword(s):  
Atpase Activity ◽  
Silver Carp ◽  
Textural Properties ◽  
Surface Hydrophobicity ◽  
Protein Hydrolysates ◽  
Control Group ◽  
Hypophthalmichthys Molitrix ◽  
Freeze Thaw ◽  
And Control ◽  
By Products

The cryoprotective effects of different amounts of protein hydrolysates prepared from by-products of silver carp using Protamex and Alcalase on surimi that were subjected to six freeze-thaw cycles were investigated. Commercial cryoprotectant (8% w/w 1:1 sucrose-sorbitol blend, SuSo) and control (without cryoprotectant) groups were used for comparison. After six freeze-thaw cycles, the lowest actomyosin extractability, Ca2+-ATPase activity and total sulfhydryl content, along with the highest surface hydrophobicity of actomyosin, were observed in the control group (P < 0.05). On the contrary, the group with addition of 2 g of hydrolysate prepared by Protamex hydrolysis (PH-2) displayed the highest actomyosin extractability, Ca2+-ATPase activity and correspondingly, lowest surface hydrophobicity of actomyosin (P < 0.05). Total sulfhydryl content of actomyosin and textural properties of heat-set surimi gels were similar between samples with PH-2 and those with SuSo (P > 0.05). Differences in molecular weight distribution, total and free amino acid compositions between the hydrolysates prepared by Protamex and Alcalase hydrolysis were possible reasons attributing to their variable cryoprotective effects on freeze-thawed surimi. Results from this study clearly support that hydrolysate prepared by Protamex hydrolysis at an appropriate amount could serve as an effective cryoprotectant without increasing the sweetness of surimi products. Furthermore, our findings suggest that the hydrolysates follow a different cryoprotection mechanism compared to SuSo (sucrose-sorbitol blend).

Antioxidant and biochemical properties of protein hydrolysates prepared from Silver carp (Hypophthalmichthys molitrix)

2008 ◽  
Vol 107 (4) ◽  
pp. 1485-1493 ◽  
Author(s):  
Shiyuan Dong ◽  
Mingyong Zeng ◽  
Dongfeng Wang ◽  
Zunying Liu ◽  
Yuanhui Zhao ◽  
...  
Keyword(s):  
Silver Carp ◽  
Biochemical Properties ◽  
Protein Hydrolysates ◽  
Hypophthalmichthys Molitrix

Effect of Superchilling Storage on Quality Characterizes and Physical Properties of Surimi from Silver Carp (Hypophthalmichthys molitrix) as Compared with Cooling Freshness Preservation

2012 ◽  
Vol 554-556 ◽  
pp. 978-984
Author(s):  
Qian Liu ◽  
Jian Chun Han ◽  
Yong Gen Zhang ◽  
Shuang Mei Li ◽  
Jing Li ◽  
...  
Keyword(s):  
Atpase Activity ◽  
Physical Properties ◽  
Storage Time ◽  
Ph Value ◽  
Silver Carp ◽  
Thiobarbituric Acid ◽  
Raw Material ◽  
Hypophthalmichthys Molitrix ◽  
Thiobarbituric Acid Reactive Substances ◽  
Fresh Products

Quality characterizes of surimi of silver carp at two different conditions of superchilling and cooling freshness preservation. The pH value, thiobarbituric acid-reactive substances (TBARS value), protein soubility and ATPase activity were studied. The result showed that pH value and TBARS value increased with the increasing of the storage time (P < 0.05), protein soubility and both ATPase activity decreased with the increasing of the storage time (P < 0.05). In general, superchilling is a good way to preserve freshness of fresh products and the raw material before processing, and also could have great effect on improving the quality characterizes of surimi and prolong its shelf life.

Influence of molecular weight on intracellular antioxidant activity of invasive silver carp ( Hypophthalmichthys molitrix ) protein hydrolysates

2015 ◽  
Vol 18 ◽  
pp. 1158-1166 ◽  
Author(s):  
Sravanthi P. Malaypally ◽  
Andrea M. Liceaga ◽  
Kee-Hong Kim ◽  
Mario Ferruzzi ◽  
Fernanda San Martin ◽  
...  
Keyword(s):  
Molecular Weight ◽  
Antioxidant Activity ◽  
Silver Carp ◽  
Protein Hydrolysates ◽  
Hypophthalmichthys Molitrix

scholarly journals Aberrant Expressional Profiling of Known MicroRNAs in the Liver of Silver Carp (Hypophthalmichthys molitrix) Following Microcystin-LR Exposure Based on samllRNA Sequencing

Toxins ◽  
2020 ◽  
Vol 12 (1) ◽  
pp. 41 ◽  
Author(s):  
Yiyi Feng ◽  
Xi Chen ◽  
Junguo Ma ◽  
Bangjun Zhang ◽  
Xiaoyu Li
Keyword(s):  
Signaling Pathway ◽  
High Throughput Sequencing ◽  
Target Genes ◽  
Liver Toxicity ◽  
Silver Carp ◽  
Wnt Signaling Pathway ◽  
Enrichment Analysis ◽  
Control Group ◽  
Hypophthalmichthys Molitrix ◽  
Multiple Organs

Microcystin-LR (MC-LR) poses a serious threat to human health due to its hepatotoxicity. However, the specific molecular mechanism of miRNAs in MC-LR-induced liver injury has not been determined. The aim of the present study was to determine whether miRNAs are regulated in MC-LR-induced liver toxicity by using high-throughput sequencing. Our research demonstrated that 53 miRNAs and 319 miRNAs were significantly changed after 24 h of treatment with MC-LR (50 and 200 μg/kg, respectively) compared with the control group. GO enrichment analysis revealed that these target genes were related to cellular, metabolic, and single-organism processes. Furthermore, KEGG pathway analysis demonstrated that the target genes of differentially expressed miRNAs in fish liver were primarily involved in the insulin signaling pathway, PPAR signaling pathway, Wnt signaling pathway, and transcriptional misregulation in cancer. Moreover, we hypothesized that 4 miRNAs (miR-16, miR-181a-3p, miR-451, and miR-223) might also participate in MC-LR-induced toxicity in multiple organs of the fish and play regulatory roles according to the qPCR analysis results. Taken together, our results may help to elucidate the biological function of miRNAs in MC-LR-induced toxicity.

scholarly journals Modulation of Biochemical and Haematological Indices of Silver Carp (Hypophthalmichthys molitrix Val.) Exposed to Toxic Cyanobacterial Water Bloom

2010 ◽  
Vol 79 (1) ◽  
pp. 135-146 ◽  
Author(s):  
Radovan Kopp ◽  
Miroslava Palíková ◽  
Stanislav Navrátil ◽  
Zdenek Kubíček ◽  
Andrea Ziková ◽  
...  
Keyword(s):  
Alkaline Phosphatase ◽  
Total Protein ◽  
Total Concentration ◽  
Pure Water ◽  
Silver Carp ◽  
Water Bloom ◽  
Control Group ◽  
Hypophthalmichthys Molitrix ◽  
Phosphorus Iron ◽  
Haematological Indices

The aim of this study was to evaluate the influence of toxic cyanobacterial water bloom on the blood indices of two-year-old silver carp (Hypophthalmichthys molitrixVal.). Experimental fish (336 ± 32 mm length, 365 ± 112 g weight) were exposed to a natural population of cyanobacterial water bloom (mainlyMicrocystis aeruginosaandM. ichthyoblabe) that contained microcystins (total concentration 133–284 μg g-1(DW), concentration in water 2.8–7.4 μg l-1). Haematological indices showed marked changes in fish exposed to the cyanobacterial population compared to the control group. Statistical evaluation of the influence of cyanobacterial water bloom on biochemical and haematological indices of silver carp showed a distinct decrease of albumin, alkaline phosphatase, cholesterol, glucose, total protein, creatinine, lactate, lactate dehydrogenase, phosphorus, iron, cholinesterase, haemoglobin, haematocrit, erythrocyte and leukocyte compared to the control. Values of alanine aminotransferase were significantly increased compared to the control. After exposure to the cyanobacterial water bloom, the silver carp were kept in pure water for monitoring the persistence of biochemical and haematological indices. Influence of the cyanobacterial population on values of albumin, alkaline phosphatase, cholesterol, total protein, glucose, creatinine, phosphorus, iron, cholinesterase, erythrocyte and haematocrit persisted up to 28 days after the end of exposure. Duration of exposure, toxicity and density of cyanobacterial water bloom had an important impact on individual biochemical and haematological indices.

scholarly journals Atrazine induced toxic impacts on the survival, behavior, and biochemical aspects of Silver carp (Hypophthalmichthys molitrix)

2021 ◽  
Author(s):  
Naireen Nayab ◽  
Syed Ata Ur Rahman Shah ◽  
Sana Ullah ◽  
Muhammad Rauf ◽  
Shandana Ali ◽  
...  
Keyword(s):  
Lipid Peroxidation ◽  
Glutathione Reductase ◽  
Muscle Tissue ◽  
Total Protein ◽  
Silver Carp ◽  
Harmful Effect ◽  
Time Dependent ◽  
Control Group ◽  
Dependent Manner ◽  
Hypophthalmichthys Molitrix

Abstract The current research work was conducted to evaluate the acute toxicity of the widely used herbicide, Atrazine on the behavioral responses and biochemical changes in different tissues of Silver carp (Hypophthalmichthys molitrix). Adult fish were exposed to different concentrations of Atrazine for different time periods. Mortality of fish was observed with increase in the concentration of Atrazine and time of exposure. The LC50 of Atrazine for 96 hours was calculated as 13.7µl/L. Fish exposed to Atrazine showed fast swimming, loss of equilibrium and balance, gulping, pale body coloration, and an excess amount of mucous secretion covered the buccal cavity and gills. Total protein contents, antioxidant enzymes including catalase (CAT), peroxidase (POD), and glutathione reductase (GR) and lipid peroxidation (LPO), were measured in the brain, gills, liver, and muscle tissues for biochemical analysis. Atrazine exposure resulted in a significant time-dependent decrease in total protein content in the liver, gills, brain, and muscle tissue of fish compared to the control group. A slight increase was observed in the activities of CAT and POD in the control group, while a significant decrease was observed in the ATZ-treated groups from 24 to 96 h. The glutathione reductase activity was significantly increased in the ATZ treated groups compared to control group. Likely, LPO level also increased in the liver, gills, brain, and muscle tissue in a time-dependent and concentration-dependent manner in ATZ treated groups compared to control group. Concludingly, our results revealed that Atrazine is toxic to fish, alter enzymes and total protein level, and lipid peroxidation level in fish. This harmful effect of Atrazine maybe generalized into environment and reduce the Silver carp population in the natural freshwater bodies.

Effects of Increases in Freshwater Temperature on Loss of Smolt Characteristics in Atlantic Salmon (Salmo salar)

1991 ◽  
Vol 48 (2) ◽  
pp. 164-169 ◽  
Author(s):  
J. Duston ◽  
R. L. Saunders ◽  
D. E. Knox
Keyword(s):  
Atlantic Salmon ◽  
Atpase Activity ◽  
Salmo Salar ◽  
Salinity Tolerance ◽  
Plasma Osmolality ◽  
Inorganic Phosphorus ◽  
Control Group ◽  
Atlantic Salmon Salmo Salar ◽  
Natural Photoperiod ◽  
And Control

Two-year-old (2+) Atlantic salmon (Salmo salar) raised under ambient temperature and simulated natural photoperiod (45°N) completed smolting between February and May, exhibiting significant increases in gill Na+, K+-ATPase activity and salinity tolerance (96 h, 37.5 ppt) but no significant changes in plasma osmolality. On May 2 the smolts were divided into four groups (each n = 80) and subjected to a rapid (2–3 h) increase in temperature from ambient (5 °C) to 10, 13, or 16 °C or remained ambient (control) which rose to 12 °C by the end of the experiment on June 5. In the 10, 13, and 16 °C groups, mean gill Na+, K+-ATPase activity (micromoles inorganic phosphorus per milligram protein per hour) declined significantly from 6.6 units on May 2 to 4.3, 3.6, and 2.3 units, respectively, on May 23. In the control group, Na+, K+-ATPase activity showed no significant changes during the study. Plasma osmolality was maintained in all groups between 290 and 304 mosmol∙kg−1. Salinity tolerance tests revealed a significant decline in survival in the 16 °C group from May 2 and in the 10 °C, 13 °C, and control groups from May 13, but no significant differences were observed among the three groups. The results support the hypothesis that increases in freshwater temperature accelerate the loss of hypoosmoregulatory capacity.

Sign in / Sign up

Export Citation Format

Share Document