Rapamycin mitigates erythrocyte membrane transport functions and oxidative stress during aging in rats

2017 ◽  
Vol 124 (1) ◽  
pp. 45-53 ◽  
Author(s):  
Abhishek Kumar Singh ◽  
Sandeep Singh ◽  
Geetika Garg ◽  
Syed Ibrahim Rizvi
Keyword(s):  
Oxidative Stress ◽  
Membrane Transport ◽  
Erythrocyte Membrane ◽  
And Oxidative Stress

scholarly journals Effects of levamisole on haematological and oxidative stress parameters in packed donkeys

2021 ◽  
Author(s):  
Babatunde Folayemi Okaiyeto ◽  
Anthony Kojo Sackey ◽  
Abdullahi Koko Mohammed ◽  
Tangang Aluwong
Keyword(s):  
Oxidative Stress ◽  
Erythrocyte Membrane ◽  
Treated Group ◽  
Membrane Stability ◽  
Strenuous Exercise ◽  
Erythrocyte Membranes ◽  
Physical Factors ◽  
Group A ◽  
Cellular Components ◽  
And Oxidative Stress

Abstract Stress can be caused by psychological, physiological, environmental and physical factors. Strenuous exercise like packing in donkeys modifies haematologic parameters. The aim of the study was to investigate the ameliorative effects of levamisole on stress, in packed donkeys. 15 adult male donkeys aged between 4 – 5 years were selected for this study, divided into groups (A, B, C) of five donkeys each: Groups A and B donkeys were the apparently healthy group; while Group C donkeys were naturally infected with Strongyle spp. All the donkeys participated in load carrying (packing) of 40 kg for 10 km. Groups B and C were treated with levamisole at 7.5 mg/kg, while Group A received no treatment prior to packing. Blood was collected from all the groups for haemogram and oxidative stress biomarker analyses. No significant effect (P > 0.05) was observed between groups: A, B and C for: respiratory rate, pulse rate and rectal temperature; haemogram, and activities of malondialdehyde concentration, superoxide dismutase and catalase. Erythrocyte membranes were osmotically stable at 0.5% NaCl in the treated group in comparison to the controls. Packing of donkeys for 10 km did not induce significant changes in vital parameters, haemogram and biomarkers of oxidative stress, but levamisole improved erythrocyte membrane stability. It was concluded that packing for 10 km did not induce any significant changes in blood cellular components and biomarker of oxidative stress, but levamisole improved erythrocyte membrane stability.

Lactoferrin-Protector against Oxidative Stress and Regulator of Glycolysis in Human Erythrocytes

2003 ◽  
Vol 58 (3-4) ◽  
pp. 256-262 ◽  
Author(s):  
Ana Maneva ◽  
Borislava Taleva ◽  
Lilia Maneva
Keyword(s):  
Oxidative Stress ◽  
Metal Ions ◽  
Erythrocyte Membrane ◽  
Thiobarbituric Acid ◽  
Glycolytic Enzyme ◽  
Membrane Receptors ◽  
Thiobarbituric Acid Reactive Substances ◽  
Transport Chain ◽  
The Media ◽  
And Oxidative Stress

Binding of lactoferrin (Lf) to its membrane receptors requires an electron for the reduction of Fe3+LF to Fe2+LF. It is possible that glyceraldehyde D3-phosphate dehydrogenase, a glycolytic enzyme part of the erythrocyte membrane, delivers that electron. Then Lf, obtaining an electron from the coenzyme NADH, might stimulate glycolysis, which requires the oxidised state of the coenzyme NAD+. Such possibility is supported by the finding that another extracellular e- acceptor - potassium ferricyanide activates glycolysis by the similar mechanism. Present results show that ferricyanide inhibited the specific 59Fe-lactoferrin binding to its erythrocyte membrane receptors. It may be assumed that ferricyanide competes with lactoferrin for an electron which leads to decrease of the binding of 59Fe-lactoferrin to its receptors. Lactoferrin (50 and 100 nm), similar to ferricyanide, increased the accumulation of lactate (respectively by 25% and 30%). These results support the assumption that ferricyanide and lactoferrin are final acceptors of a common electron transport chain connected with the regulation of glycolysis.We established an antioxidative effect of lactoferrin on erythrocytes, which was expressed as: a) an influence on content and on activity of intracellular antioxidants - namely an enhancement of the content of reduced glutathione; b) a decreased content both of products of lipid peroxidation (thiobarbituric acid reactive substances) and hemolysis under normal conditions and oxidative stress.Lactoferrin is capable to bind metal ions and thus to block their catalytic participation in the oxidative disturbances of the membrane. In most of our experiments there were no metal ions in the incubation mixtures (except those stimulating oxidative stress). Our results showed that Lf limited both the generation of thiobarbituric acid reactive substances and hemolysis in the absence of metal ions in the media, as well as in their presence. These facts suggest that probably the antioxidative property of lactoferrin is glycolysis stimulation, leading to increased formation of ATP, which is necessary to maintain the ion gradient, membrane potential and morphology of the erythrocyte.

Association between calcitriol and paricalcitol with oxidative stress in patients with hemodialysis

2020 ◽  
pp. 1-8
Author(s):  
Hasan Haci Yeter ◽  
Berfu Korucu ◽  
Elif Burcu Bali ◽  
Ulver Derici
Keyword(s):  
Oxidative Stress ◽  
Vitamin D ◽  
Antioxidant Status ◽  
Total Antioxidant Status ◽  
Stress Index ◽  
Total Oxidant Status ◽  
Vitamin D Analog ◽  
Total Antioxidant ◽  
Oxidant Status ◽  
And Oxidative Stress

Abstract. Background: The pathophysiological basis of chronic kidney disease and its complications, including cardiovascular disease, are associated with chronic inflammation and oxidative stress. We investigated the effects of active vitamin D (calcitriol) and synthetic vitamin D analog (paricalcitol) on oxidative stress in hemodialysis patients. Methods: This cross-sectional study was composed of 83 patients with a minimum hemodialysis vintage of one year. Patients with a history of any infection, malignancy, and chronic inflammatory disease were excluded. Oxidative markers (total oxidant and antioxidant status) and inflammation markers (C-reactive protein and interleukin-6) were analyzed. Results: A total of 47% (39/83) patients were using active or analog vitamin D. Total antioxidant status was significantly higher in patients with using active or analog vitamin D than those who did not use (p = 0.006). Whereas, total oxidant status and oxidative stress index were significantly higher in patients with not using vitamin D when compared with the patients who were using vitamin D preparation (p = 0.005 and p = 0.004, respectively). On the other hand, total antioxidant status, total oxidant status, and oxidative stress index were similar between patients who used active vitamin D or vitamin D analog (p = 0.6; p = 0.4 and p = 0.7, respectively). Conclusion: The use of active or selective vitamin D analog in these patients decreases total oxidant status and increases total antioxidant status. Also, paricalcitol is as effective as calcitriol in decreasing total oxidant status and increasing total antioxidant status in patients with chronic kidney disease.

Effects of Off-Pump Versus On-Pump Coronary Artery Bypass Grafting: Apoptosis, Inflammation, and Oxidative Stress

2014 ◽  
Vol 17 (5) ◽  
pp. 271 ◽  
Author(s):  
Murat Bicer ◽  
Tunay Senturk ◽  
Murat Yanar ◽  
Ahmet Tutuncu ◽  
Arzu Yilmaztepe Oral ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Ischemia Reperfusion ◽  
Artery Bypass ◽  
Bypass Grafting ◽  
Artery Bypass Grafting ◽  
Cabg Surgery ◽  
Off Pump ◽  
Pump Cabg ◽  
Hs Crp ◽  
And Oxidative Stress

<strong>Background</strong>: It has been suggested that off-pump coronary<br />artery bypass grafting (CABG) surgery reduces myocardial<br />ischemia-reperfusion injury, postoperative systemic<br />inflammatory response, and oxidative stress. The aim of this<br />study was to measure serum malondialdehyde (MDA), highsensitivity<br />C-reactive protein (hs-CRP), M30, and M65 levels<br />and to investigate the relationship between M30 levels and<br />oxidative stress and inflammation in patients undergoing onand<br />off-pump CABG surgery.<br /><strong>Methods</strong>: Fifty patients were randomly assigned to onpump<br />or off-pump CABG surgery (25 patients off-pump and<br />25 on-pump CABG surgery), and blood samples were collected<br />prior to surgery, and 30 minutes, 60 minutes, 6 hours,<br />and 24 hours after CABG surgery.<br /><strong>Results</strong>: Compared to the on-pump group, serum MDA<br />levels at 30 minutes, 60 minutes, 6 hours, and 24 hours after<br />the CABG surgery were significantly lower in the off-pump<br />group (P = .001, P = .001, P = .001, and P = .001, respectively).<br />Serum M30 levels were found to be elevated in both groups,<br />returning to baseline at 24 hours. When compared to baseline,<br />the hs-CRP level reached its peak at 24 hours at 13.28 ±<br />5.32 mg/dL in the on-pump group, and 15.44 ± 4.02 mg/dL<br />in the off-pump group.<br /><strong>Conclusion</strong>: CABG surgery is associated with an increase<br />in inflammatory markers and serum M30 levels, indicating<br />epithelial/endothelial apoptosis in the early period.

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