The classification of almonds (Prunus dulcis) by country and variety using UHPLC-HRMS-based untargeted metabolomics

Almonds show a great variability in their chemical composition. This variability is a result of the existence of a diverse range of almond cultivars, the self-incompatibility of most almond cultivars, and the heterogeneous harvesting conditions found around the different locations where almons are grown. In the last years, the discrimination among almond cultivars has been the focal point of some research studies to avoid fraud in protected geographical indications in almond products and also for selecting the best cultivars for a specific food application or the most interesting ones from a nutritional point of view. In this work, a revision of the recent research works related to the chemical characterization and classification of almond cultivars from different geographical origins has been carried out. The content of macronutrients, tocopherols, phytosterols, polyphenols, minerals, amino acids, and volatile compounds together with DNA fingerprint have been reported as possible cultivar and origin markers. The analysis of the results showed that no individual almond compound could be considered a universal biomarker to find differences among different almond cultivars. Hence, an adequate selection of variables or the employment of metabolomics and the application of multivariate statistical techniques is necessary when classification studies are carried out to obtain valuable results. Meanwhile, DNA fingerprinting is the perfect tool for compared cultivars based on their genetic origin.

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Application of DNA fingerprinting for the classification of selected almond [Prunus dulcis (Miller) D. A. Webb] cultivars

Australian Journal of Experimental Agriculture ◽

10.1071/ea00061 ◽

2000 ◽

Vol 40 (7) ◽

pp. 995 ◽

Cited By ~ 8

Author(s):

F. M. Woolley ◽

G. G. Collins ◽

M. Sedgley

Keyword(s):

Middle East ◽

Prunus Dulcis ◽

Group Method ◽

Randomly Amplified Polymorphic Dna ◽

Breeding Lines ◽

Pair Group ◽

Commercial Cultivars ◽

Unweighted Pair Group Method ◽

Prunus Webbii

Almond cultivars developed in Australia are thought to have descended from 2 breeding lines, 1 from hard-shelled Spanish/Jordan types, and the other from paper-shell Californian types. However, the precise derivation of many individual Australian cultivars is uncertain. Randomly amplified polymorphic DNA (RAPD) was used to estimate the genetic similarities between 50 accessions of almond cultivars derived from Australia, California, Europe and the Middle East, and individual accessions of Prunus orientalis (Miller) D. A. Webb and Prunus webbii (Spach) Vieh. Amplification products were analysed using the simple matching coefficient and the unweighted pair group method with arithmetic averages to cluster individuals into a dendrogram. Cultivars known to have originated in Europe or the Middle East clustered in a different group from those known to have originated in California confirming the 2 suspected breeding lines. The origin of some common Australian commercial cultivars was inferred by their placement on the dendrogram, and the possible parentage of some Australian selections is discussed.

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Untargeted Metabolomics Analysis by UHPLC-MS/MS of Soybean Plant in a Compatible Response to Phakopsora pachyrhizi Infection

Metabolites ◽

10.3390/metabo11030179 ◽

2021 ◽

Vol 11 (3) ◽

pp. 179

Author(s):

Evandro Silva ◽

José Perez da Graça ◽

Carla Porto ◽

Rodolpho Martin do Prado ◽

Estela Nunes ◽

...

Keyword(s):

Principal Component ◽

Untargeted Metabolomics ◽

Soybean Rust ◽

Soybean Plant ◽

Phakopsora Pachyrhizi ◽

Crop Disease ◽

Soybean Plants ◽

Fatty Acyls

Phakopsora pachyrhizi is a biotrophic fungus, causer of the disease Asian Soybean Rust, a severe crop disease of soybean and one that demands greater investment from producers. Thus, research efforts to control this disease are still needed. We investigated the expression of metabolites in soybean plants presenting a resistant genotype inoculated with P. pachyrhizi through the untargeted metabolomics approach. The analysis was performed in control and inoculated plants with P. pachyrhizi using UHPLC-MS/MS. Principal component analysis (PCA) and the partial least squares discriminant analysis (PLS-DA), was applied to the data analysis. PCA and PLS-DA resulted in a clear separation and classification of groups between control and inoculated plants. The metabolites were putative classified and identified using the Global Natural Products Social Molecular Networking platform in flavonoids, isoflavonoids, lipids, fatty acyls, terpenes, and carboxylic acids. Flavonoids and isoflavonoids were up-regulation, while terpenes were down-regulated in response to the soybean–P. pachyrhizi interaction. Our data provide insights into the potential role of some metabolites as flavonoids and isoflavonoids in the plant resistance to ASR. This information could result in the development of resistant genotypes of soybean to P. pachyrhizi, and effective and specific products against the pathogen.

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Note on the spectral classification of carbon stars in the photographic infrared region

Symposium - International Astronomical Union ◽

10.1017/s0074180900053080 ◽

1966 ◽

Vol 24 ◽

pp. 21-23

Author(s):

Y. Fujita

Keyword(s):

Infrared Region ◽

Spectral Classification ◽

Carbon Stars

We have investigated the spectrograms (dispersion: 8Å/mm) in the photographic infrared region fromλ7500 toλ9000 of some carbon stars obtained by the coudé spectrograph of the 74-inch reflector attached to the Okayama Astrophysical Observatory. The names of the stars investigated are listed in Table 1.

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Diagnostic Value of Electron Microscopy in Soft Tissue Tumors

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100068096 ◽

1970 ◽

Vol 28 ◽

pp. 220-221

Author(s):

Gerald Fine ◽

Azorides R. Morales

Keyword(s):

Cardiac Myxoma ◽

Osteogenic Sarcoma ◽

Diagnostic Value ◽

Soft Tissue Tumors ◽

Amelanotic Melanoma ◽

Growth Patterns ◽

Light Microscopic Level ◽

Mesenchymal Tumors ◽

Good Agreement

For years the separation of carcinoma and sarcoma and the subclassification of sarcomas has been based on the appearance of the tumor cells and their microscopic growth pattern and information derived from certain histochemical and special stains. Although this method of study has produced good agreement among pathologists in the separation of carcinoma from sarcoma, it has given less uniform results in the subclassification of sarcomas. There remain examples of neoplasms of different histogenesis, the classification of which is questionable because of similar cytologic and growth patterns at the light microscopic level; i.e. amelanotic melanoma versus carcinoma and occasionally sarcoma, sarcomas with an epithelial pattern of growth simulating carcinoma, histologically similar mesenchymal tumors of different histogenesis (histiocytoma versus rhabdomyosarcoma, lytic osteogenic sarcoma versus rhabdomyosarcoma), and myxomatous mesenchymal tumors of diverse histogenesis (myxoid rhabdo and liposarcomas, cardiac myxoma, myxoid neurofibroma, etc.)

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Ultrastructure of mesotheliomas

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100110775 ◽

1984 ◽

Vol 42 ◽

pp. 98-101

Author(s):

Irving Dardick

Keyword(s):

Electron Microscopy ◽

Latent Period ◽

Spindle Cell ◽

Spindle Cell Sarcoma ◽

Metastatic Adenocarcinoma ◽

Cell Sarcoma ◽

Cytological Features ◽

Industrial Use ◽

Degree Of Differentiation

With the extensive industrial use of asbestos in this century and the long latent period (20-50 years) between exposure and tumor presentation, the incidence of malignant mesothelioma is now increasing. Thus, surgical pathologists are more frequently faced with the dilemma of differentiating mesothelioma from metastatic adenocarcinoma and spindle-cell sarcoma involving serosal surfaces. Electron microscopy is amodality useful in clarifying this problem.In utilizing ultrastructural features in the diagnosis of mesothelioma, it is essential to appreciate that the classification of this tumor reflects a variety of morphologic forms of differing biologic behavior (Table 1). Furthermore, with the variable histology and degree of differentiation in mesotheliomas it might be expected that the ultrastructure of such tumors also reflects a range of cytological features. Such is the case.

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Interpreting HVEM of muscle-cell impulse networks

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s042482010012103x ◽

1992 ◽

Vol 50 (1) ◽

pp. 126-127

Author(s):

Paul DeCosta ◽

Kyugon Cho ◽

Stephen Shemlon ◽

Heesung Jun ◽

Stanley M. Dunn

Keyword(s):

Structural Analysis ◽

Muscle Cell ◽

Electron Micrographs ◽

Relative Size ◽

Automatic Classification ◽

Nerve Fibers ◽

Analysis Algorithm ◽

Structural Networks

Introduction: The analysis and interpretation of electron micrographs of cells and tissues, often requires the accurate extraction of structural networks, which either provide immediate 2D or 3D information, or from which the desired information can be inferred. The images of these structures contain lines and/or curves whose orientation, lengths, and intersections characterize the overall network.Some examples exist of studies that have been done in the analysis of networks of natural structures. In, Sebok and Roemer determine the complexity of nerve structures in an EM formed slide. Here the number of nodes that exist in the image describes how dense nerve fibers are in a particular region of the skin. Hildith proposes a network structural analysis algorithm for the automatic classification of chromosome spreads (type, relative size and orientation).

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Dual stain kit for the microscopic gram classification of ocular infection bacteria

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100147089 ◽

1993 ◽

Vol 51 ◽

pp. 248-249

Author(s):

Jacob S. Hanker ◽

Dale N. Holdren ◽

Kenneth L. Cohen ◽

Beverly L. Giammara

Keyword(s):

Contact Lenses ◽

Ocular Infection ◽

Gram Negative Bacteria ◽

Gram Negative ◽

Gram Staining ◽

Ocular Infections ◽

Different Types ◽

Culture Positive ◽

Increased Susceptibility

Keratitis and conjunctivitis (infections of the cornea or conjunctiva) are ocular infections caused by various bacteria, fungi, viruses or parasites; bacteria, however, are usually prominent. Systemic conditions such as alcoholism, diabetes, debilitating disease, AIDS and immunosuppressive therapy can lead to increased susceptibility but trauma and contact lens use are very important factors. Gram-negative bacteria are most frequently cultured in these situations and Pseudomonas aeruginosa is most usually isolated from culture-positive ulcers of patients using contact lenses. Smears for staining can be obtained with a special swab or spatula and Gram staining frequently guides choice of a therapeutic rinse prior to the report of the culture results upon which specific antibiotic therapy is based. In some cases staining of the direct smear may be diagnostic in situations where the culture will not grow. In these cases different types of stains occasionally assist in guiding therapy.

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Ultrastructural Classification of Pituary Adenomas

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100158832 ◽

1990 ◽

Vol 48 (3) ◽

pp. 258-258

Author(s):

S. Arumugam ◽

Sarasa Bharati Arumugam

Keyword(s):

Cytoplasmic Organelle ◽

Null Cell ◽

Cell Adenoma ◽

Mixed Cell ◽

Light Microscope Level ◽

Null Cell Adenoma ◽

Growth Hormone Cell ◽

Ultrastructural Characteristics ◽

Organelle Morphology

Adenoaas of the pituitary are no longer classified based on their tinctorial affinity to dyes. With the advent of the newer methods of sophisticated technology, it is now possible to classify. These depending upon the type of hormone secreted based either on histochemical techniques or on ultrastructural characteristics. The latter provides an insight into the cytoplasmic organelle morphology which offers a delightful feast to the eye as well.This paper presents the ultrastructural characters of the pituitary adenoma as seen in Madras. 171 adenomas (124 males and 47 females) were seen during 1972-1989, classified at the light microscope level as 159 chromophobe, 2 basophilic, 4 eosinophilic and 6 mixed adenomas.Ultrastructural examination showed that the sparsely granular prolactin cell adenoma is the commonest adenoma to be encountered closely followed by the growth hormone cell adenoma, null cell adenoma, the mixed cell adenoma and others.

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