scholarly journals Synergistic effect of silver nanoparticles and polymyxin B against biofilm produced by Pseudomonas aeruginosa isolates of pus samples in vitro

2019 ◽  
Vol 47 (1) ◽  
pp. 2465-2472 ◽  
Author(s):  
Muhammad Salman ◽  
Rizwana Rizwana ◽  
Hayat Khan ◽  
Iqbal Munir ◽  
Muhammad Hamayun ◽  
...  
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Silver Nanoparticles ◽  
Synergistic Effect ◽  
Polymyxin B

In vitro analysis of green fabricated silver nanoparticles (AgNPs) against Pseudomonas aeruginosa PA14 biofilm formation, their application on urinary catheter

2021 ◽  
Vol 151 ◽  
pp. 106058
Author(s):  
Felix LewisOscar ◽  
Chari Nithya ◽  
Sasikumar Vismaya ◽  
Manivel Arunkumar ◽  
Arivalagan Pugazhendhi ◽  
...  
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Silver Nanoparticles ◽  
Biofilm Formation ◽  
Urinary Catheter ◽  
Vitro Analysis ◽  
In Vitro Analysis

scholarly journals Biological Characterization of Endotoxins Released from Antibiotic-Treated Pseudomonas aeruginosa and Escherichia coli

1998 ◽  
Vol 42 (5) ◽  
pp. 1015-1021 ◽  
Author(s):  
Teruo Kirikae ◽  
Fumiko Kirikae ◽  
Shinji Saito ◽  
Kaoru Tominaga ◽  
Hirohi Tamura ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Pseudomonas Aeruginosa ◽  
Lipid A ◽  
Biological Activities ◽  
Polymyxin B ◽  
Mitogen Activated Protein Kinase ◽  
No Production ◽  
Endotoxic Activity

ABSTRACT The supernatants taken from Pseudomonas aeruginosa andEscherichia coli cultures in human sera or chemically defined M9 medium in the presence of ceftazidime (CAZ) contained high levels of endotoxin, while those taken from the same cultures in the presence of imipenem (IPM) yielded a very low level of endotoxin. The biological activities of endotoxin in the supernatants were compared with those of phenol water-extracted lipopolysaccharide (LPS). The endotoxin released from the organisms as a result of CAZ treatment (CAZ-released endotoxin) contained a large amount of protein. The protein, however, lacked endotoxic activity, since the endotoxin did not show any in vivo toxic effects in LPS-hyporesponsive C3H/HeJ mice sensitized with d-(+)-galactosamine (GalN) or any activation of C3H/HeJ mouse macrophages in vitro. The activities of CAZ- and IPM-released endotoxin (as assessed by a chromogenicLimulus test) were fundamentally the same as those ofP. aeruginosa LPS, since their regression lines were parallel. The CAZ-released endotoxin was similar to purified LPS with respect to the following biological activities in LPS-responsive C3H/HeN mice and LPS-hyporesponsive C3H/HeJ mice: lethal toxicity in GalN-sensitized mice, in vitro induction of tumor necrosis factor- and NO production by macrophages, and mitogen-activated protein kinase activation in macrophages. The macrophage activation by CAZ-released endotoxin as well as LPS was mainly dependent on the presence of serum factor and CD14 antigen. Polymyxin B blocked the activity. These findings indicate that the endotoxic activity of CAZ-released endotoxin is due primarily to LPS (lipid A).

scholarly journals Antibacterial ativity of biosynthesized silver nanoparticles against Pseudomonas aeruginosa in vitro

2017 ◽  
Vol 41 (1) ◽  
pp. 60-65
Author(s):  
Ahmad N. A. Salih ◽  
Mohammad J. Eesa
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Pseudomonas Aeruginosa ◽  
Silver Nanoparticles ◽  
Antibacterial Activity ◽  
Silver Sulfadiazine ◽  
Diffusion Method ◽  
Olive Leaves ◽  
Scanning Electron

     This study was conducted for the synthesis of silver nanoparticles by using olive leaves aqueous extract and evaluate its antibacterial activity against Pseudomonas aeruginosa in vitro. The synthesis and characterization of silver nanoparticles was confirmed by Ultra Violet Visible – spectrophotometer and Scanning Electron Microscopy. Well diffusion method was used to show the antibacterial action of silver nanoparticles against Pseudomonas aeruginosa in vitro in comparison with standard antibacterial silver sulfadiazine by using different concentrations of each agent ranged from 12.5-200 μg/ml. The results of this study showed it possible to produce silver nanoparticles in eco-friendly and easy process and UV-Visible absorption spectra of the silver nanoparticles revealed maximum absorbance at 420 and 430 nm. The Scanning Electron Microscopy analysis demonstrated the mean of the silver particles diameter was 26 nm. The antibacterial findings of the synthesized silver nanoparticles against Pseudomonas aeruginosa in vitro showed that the silver nanoparticles were more effective than silver sulfadiazine against Pseudomonas aeruginosa. It could be concluded that olive leaves extract can be used effectively in the production of silver nanoparticles and these synthesized nanoparticles had considerable antibacterial activity against Pseudomonas aeruginosa in vitro.                                                        

scholarly journals Synergy of Silver Nanoparticles and Aztreonam against Pseudomonas aeruginosa PAO1 Biofilms

2014 ◽  
Vol 58 (10) ◽  
pp. 5818-5830 ◽  
Author(s):  
Marc B. Habash ◽  
Amber J. Park ◽  
Emily C. Vis ◽  
Robert J. Harris ◽  
Cezar M. Khursigara
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Silver Nanoparticles ◽  
Bacterial Infections ◽  
Antimicrobial Agents ◽  
Cell Envelope ◽  
Antimicrobial Properties ◽  
Planktonic Cell ◽  
Chronic Infections ◽  
Content Type

ABSTRACTPathogenic bacterial biofilms, such as those found in the lungs of patients with cystic fibrosis (CF), exhibit increased antimicrobial resistance, due in part to the inherent architecture of the biofilm community. The protection provided by the biofilm limits antimicrobial dispersion and penetration and reduces the efficacy of antibiotics that normally inhibit planktonic cell growth. Thus, alternative antimicrobial strategies are required to combat persistent infections. The antimicrobial properties of silver have been known for decades, but silver and silver-containing compounds have recently seen renewed interest as antimicrobial agents for treating bacterial infections. The goal of this study was to assess the efficacy of citrate-capped silver nanoparticles (AgNPs) of various sizes, alone and in combination with the monobactam antibiotic aztreonam, to inhibitPseudomonas aeruginosaPAO1 biofilms. Among the different sizes of AgNPs examined, 10-nm nanoparticles were most effective in inhibiting the recovery ofP. aeruginosabiofilm cultures and showed synergy of inhibition when combined with sub-MIC levels of aztreonam. Visualization of biofilms treated with combinations of 10-nm AgNPs and aztreonam indicated that the synergistic bactericidal effects are likely caused by better penetration of the small AgNPs into the biofilm matrix, which enhances the deleterious effects of aztreonam against the cell envelope ofP. aeruginosawithin the biofilms. These data suggest that small AgNPs synergistically enhance the antimicrobial effects of aztreonam againstP. aeruginosain vitro, and they reveal a potential role for combinations of small AgNPs and antibiotics in treating patients with chronic infections.

scholarly journals In Vitro Activity Comparison of Ceftazidime–Avibactam and Aztreonam–Avibactam Against Bloodstream Infections With Carbapenem-Resistant Organisms in China

2021 ◽  
Vol 11 ◽  
Author(s):  
Wei Yu ◽  
Luying Xiong ◽  
Qixia Luo ◽  
Yunbo Chen ◽  
Jinru Ji ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Pseudomonas Aeruginosa ◽  
Bloodstream Infections ◽  
Polymyxin B ◽  
Resistance Rate ◽  
In Vitro Activity ◽  
Carbapenem Resistant ◽  
Resistance Rates ◽  
Activity Comparison

ObjectivesThe aim of this work was to investigate the activity of ceftazidime–avibactam (CZA) and aztreonam–avibactam (AZA) against bloodstream infections caused by carbapenem-resistant organisms (CROs).MethodsNon-duplicate CROs, including 56 carbapenem-resistant Escherichia coli (CR-Eco), 318 carbapenem-resistant Klebsiella pneumoniae (CR-Kpn), and 65 carbapenem-resistant Pseudomonas aeruginosa (CR-Pae), were collected using the Blood Bacterial Resistant Investigation Collaborative System (BRICS) program in China. The minimum inhibitory concentrations (MICs) of 24 antibiotics were tested. Carbapenemase genes were amplified for CZA-resistant CROs by PCR. The MICs of CZA and AZA were further determined with avibactam at 8 and 16 mg/L, respectively.ResultsThe resistance rate of polymyxin B against CROs was less than 5%. Only one CR-Kpn was resistant to tigecycline. The resistance rates of CZA against CR-Eco, CR-Kpn, and CR-Pae were 75.0%, 12.6%, and 18.5%, respectively. The MIC90 values of AZA against CR-Eco, CR-Kpn, and CR-Pae were 2/4, 1/4, and 64/4 mg/L, respectively. Among the CZA-resistant CROs, 42 (100%) CR-Eco, 24 (60%) CR-Kpn, and 1 (8.3%) CR-Pae isolates harbored metallo-β-lactamase genes. The increase of avibactam concentration enhanced the susceptibility of CZA and AZA against CROs, especially for CR-Eco and CR-Kpn.ConclusionsThe in vitro activity of AZA was superior to that of CZA against CR-Eco and CR-Kpn, whereas CZA showed better effect against CR-Pae.

scholarly journals In vitro Study on the Efficacy of Silver Nanoparticles against Metallo-Beta-Lactamase and Biofilm Producing Pseudomonas aeruginosa Isolates

2019 ◽  
Vol 8 (06) ◽  
pp. 2931-2948
Author(s):  
Marwa Gamal Bahey ◽  
Abou El Fetouh Eid El Enany ◽  
Mohamed Ismaeil Abd El Hamid ◽  
Aziza Mahmoud ◽  
Has san ◽  
...  
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Silver Nanoparticles ◽  
In Vitro Study ◽  
Vitro Study ◽  
Beta Lactamase

scholarly journals Comparative study of in vitro activities of polymyxin B commercial products on Pseudomonas aeruginosa isolated from hospitalized patients

2021 ◽  
Vol 62 (3) ◽  
pp. 270-279
Author(s):  
Rezvan Goodarzi ◽  
Farhad Farahani ◽  
Mahdane Roshani ◽  
Mohammad Taheri ◽  
Babak Asghari
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Bacterial Infections ◽  
Susceptibility Testing ◽  
Renal Toxicity ◽  
Polymyxin B ◽  
Microbiological Activity ◽  
Commercial Products ◽  
Gram Negative ◽  
In Vitro Antibacterial Activity

Introduction: Polymyxin B has been applied as one of the last-resort antibiotics for the treatment of multidrug resistance among Gram-negative bacterial infections. Due to side effects such as renal toxicity, the use of polymyxin is associated with limitations. The present study evaluates in vitro antibacterial activity of a number of polymyxin B commercial products against Pseudomonas aeruginosa. Methods: This study included 63 non-duplicated P. aeruginosa isolates examined for in vitro polymyxin B susceptibility testing using the following powder disks: polymyxin B sulfate, otosporin, Poly-Mxb, and Myxacort. MIC50 and MIC90 have also been identified for polymyxin B antibiotics. Results: Myxacort had functional activity against most P. aeruginosa isolates, and only seven isolates had a relatively high MIC. The activities of Poly-MXb and Myxacort were the same as otosporin. Conclusions: Our findings revealed that the national generic polymyxin B product (Myxacort), and two external products (Otosporin, Poly-MXb) are similar in terms of microbiological activity.

Lokalantibiotika in der Ulkustherapie

Phlebologie ◽  
1998 ◽  
Vol 27 (01) ◽  
pp. 25-31 ◽  
Author(s):  
A. Becker ◽  
M. Gloor ◽  
L. Lehmann
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Polymyxin B ◽  
Ulcus Cruris ◽  
Klebsiella Spp

Zusammenfassung Ziel der vorliegenden Untersuchung war es, die Resistenzsituation gegen Lokalantibiotika des in der Therapie des Ulcus cruris relevanten Keimspektrums zu überprüfen. Methodik: Aus 750 Wundabstrichen wurden die Bakterien isoliert und hinsichtlich ihrer Empfindlichkeit gegen Lokalantibiotika getestet. Ergebnisse: Staphylokokken zeigten sich meist empfindlich gegen Bacitracin, Tetracyclin, Gentamicin, Neomycin, Erythromycin und Fusidinsäure. Streptokokken waren in allen Fällen erythromycin- und zumeist bacitracinempfindlich. Proteus spp. und Klebsiella spp. waren gegen Gentamicin und Neomycin, Klebsiellen zusätzlich gegen Polymyxin B empfindlich. Enterokokken zeigten sich nur gegen Fusidinsäure und Bacitracin sensibel. Bei Pseudomonas aeruginosa ließ sich bei weitgehender Unwirksamkeit der anderen Antibiotika eine Empfindlichkeit gegen Polymyxin B nachweisen. Schlußfolgerungen: Aufgrund der vorliegenden Ergebnisse ist eine differenzierte Therapie mit topischen Antibioka nach Keimdifferenzierung möglich, auch wenn in der Praxis eine Resistenztestung gegen Lokalantibiotika nicht durchführbar ist. Die klinische Effizienz kann bei einem Teil der Fälle besser sein, als es die In-vitro-Resistenzen erwarten lassen.

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