scholarly journals Differential organization of desmin and vimentin in muscle is due to differences in their head domains.

1994 ◽  
Vol 126 (2) ◽  
pp. 445-456 ◽  
Author(s):  
R B Cary ◽  
M W Klymkowsky
Keyword(s):  
Epithelial Cells ◽  
Plasmid Dna ◽  
The Body ◽  
The Other ◽  
Chimeric Proteins ◽  
Head Region ◽  
Other Hand ◽  
Low Levels ◽  
Filament Network ◽  
Constructed Plasmids

In most myogenic systems, synthesis of the intermediate filament (IF) protein vimentin precedes the synthesis of the muscle-specific IF protein desmin. In the dorsal myotome of the Xenopus embryo, however, there is no preexisting vimentin filament system and desmin's initial organization is quite different from that seen in vimentin-containing myocytes (Cary and Klymkowsky, 1994. Differentiation. In press.). To determine whether the organization of IFs in the Xenopus myotome reflects features unique to Xenopus or is due to specific properties of desmin, we used the injection of plasmid DNA to drive the synthesis of vimentin or desmin in myotomal cells. At low levels of accumulation, exogenous vimentin and desmin both enter into the endogenous desmin system of the myotomal cell. At higher levels exogenous vimentin forms longitudinal IF systems similar to those seen in vimentin-expressing myogenic systems and massive IF bundles. Exogenous desmin, on the other hand, formed a reticular IF meshwork and non-filamentous aggregates. In embryonic epithelial cells, both vimentin and desmin formed extended IF networks. Vimentin and desmin differ most dramatically in their NH2-terminal "head" regions. To determine whether the head region was responsible for the differences in the behavior of these two proteins, we constructed plasmids encoding chimeric proteins in which the head of one was attached to the body of the other. In muscle, the vimentin head-desmin body (VDD) polypeptide formed longitudinal IFs and massive IF bundles like vimentin. The desmin head-vimentin body (DVV) polypeptide, on the other hand, formed IF meshworks and non-filamentous structures like desmin. In embryonic epithelial cells DVV formed a discrete filament network while VDD did not. Based on the behavior of these chimeric proteins, we conclude that the head domains of vimentin and desmin are structurally distinct and not interchangeable, and that the head domain of desmin is largely responsible for desmin's muscle-specific behaviors.

The Ability to Differentiate, and the Size of Regenerated Cells, after repeated Regeneration in Spongilla lacustris

1953 ◽  
Vol s3-94 (26) ◽  
pp. 177-184
Author(s):  
H. V. BRONDSTED
Keyword(s):  
Epithelial Cells ◽  
The Body ◽  
The Other ◽  
Entire Body ◽  
Other Hand ◽  
The Individual ◽  
Basal Epithelial Cells

The findings presented here have a bearing on regeneration in general. They show that even in simply organized animals, the number of totipotent cells which are able to differentiate decreases as the individual ages. In older specimens it takes longer for the totipotent cells to differentiate than in younger ones; at the same time the nuclear and cytoplasmic volume of these cells is reduced. The expanded basal epithelium of sponges germinating from gemmules is an organ necessary to establish the tension in the body which is indispensable for the functioning of the sponge. Sponges that have germinated from gemmules can be forced to regenerate a basal epithelium. The materials for this regeneration is furnished by the archaeocytes, which are embryonic, totipotent amoebocytes. The number of archaeocytes that are able to perform this regeneration decreases with time, i.e. as the differentiation of the entire body proceeds. On the other hand, the ability of the sponge to expand repeatedly on its own basal epithelium after being pushed away from it is limited only by the onset of cytolysis. The ability of the archaeocytes to regenerate new typical basal epithelial cells is reduced after repeated regeneration. The size of both nucleus and cytoplasm is reduced more and more during repeated regeneration. The nucleo-cytoplasmic ratio is thus kept fairly constant.

scholarly journals CHEMICAL-BASED INSPIRATIONAL THEORETICALLY LABORATORY EXPERIMENTAL TREATMENTS FOR SUGAR-MALABSORPTION DISEASES.

2019 ◽  
Vol 8 (4) ◽  
Author(s):  
Ahmed Y. Azzam
Keyword(s):  
Acetic Acid ◽  
Small Intestine ◽  
Epithelial Cells ◽  
Gluconic Acid ◽  
Gastric Fluid ◽  
The Body ◽  
The Other ◽  
Good Effect ◽  
Other Hand ◽  
Sugar Source

The process of glucose absorption inside the body is initiated in stomach like any type of micro-chemical nutrients but specially in , epithelial cells lining small intestine. though Na+/glucose cotransporter (SGLT1) electrochemically, other transporters and sometimes using intestinal polypeptides involved. Dysfunction of epithelial cells of small intestine, transporters or in malignant cases in, absorption intestinal polypeptides leads to a rare disease called Glucose-galactose malabsorption. In this disease absorption of sugar molecules is not done probably it's accompanied baby born genetic disease. Which leads to precipitation of large sugar molecules not absorbed. In other disease that called Hypochlorhydria. Which is lack of HCl production due Pernicious anemia, surgical operations in digestive system, H.Pylori, gastric bypass, VIPomas, Hypothyroidism, Radiations to GIT system and gastric cancer could lead to production of very small amounts of HCl which also in some cases ( Not All ) has effect on absorption of sugar from gastric fluid. In this publication I'll focus more on Hypochlorhydria sugar-malabsorption side-effect in some patients who may suffer from it. In the other hand, I'll try to involve Glucose-galactose malabsorption disease but it will be limited in the experiments because, this disease is due to genetic mutations not due to gained pathological factors during life. In recent researches in highly accurate simulated chemistry lab in In-Vitro experiments to keep out from hazards reactions of real-life chemistry lab chemical medication experiments results . I've found that D-Gluconic acid when manufactured as sugar source instead of direct sugar has a very good effect for people who is suffering from sugar malabsorption caused of gastric fluid problems. It shown it's better ability to get soluble easily in the gastric pathway and stomach fluid. Acetic acid and Calcium acetate shown that they have good effect for Hypochlorhydria patients with sugar. They help on faster sugar absorbing and establishing sugar bonds before getting absorbed which makes it easier to digest. On the other hand Aluminum high doses shown that it may be a struggling source for people who suffer from gastric fluid problems like Hypochlorhydria so it's recommended to reduce and take care of taking doses of aluminum. For Glucose-galactose malabsorption disease patients. D-Gluconic acid can be used as a secondary-stabilizer disease reducer in addition to acetic acid for helping to break sugar bonds if the patient needed to get sugar source for emergency cases to make it easier for some mild cases to digest but, it can't be used for long time because it's not so much effective but it's a disease partial reducer for emergency sugar needed cases. It shown that it may help in some of mild cases severe cases aren't included yet in this experiment since it has no effect on them at most.

P0011THE ROLE OF AQUAPORIN-11 (AQP11) IN THE THYMUS DEVELOPMENT

2020 ◽  
Vol 35 (Supplement_3) ◽  
Author(s):  
Kei Masaka ◽  
Yasuko Tanaka ◽  
Kenichi Ishibashi
Keyword(s):  
Epithelial Cells ◽  
Western Blot ◽  
Cell Survival ◽  
Signaling Pathway ◽  
Microarray Analysis ◽  
The Body ◽  
The Other ◽  
Rt Pcr ◽  
Wild Mice ◽  
Other Hand

Abstract Background and Aims Some aquaporins transport not only water but also small non-ionic molecules such as glycerol. In fact, aquaporin-9 (AQP9) has been shown to transport glycerol which is critical to support memory T cell survival through metabolic fitness (Cui G et al. Cell. 161:750, 2015). As AQP11 is widely expressed and transport glycerol as well as water, AQP11 may also play a role in immunological system, which will expand the repertoire of the aquaporin research field. Method The AQP11 expression in the thymus was examined by RT-PCR, Western blot and immunohistochemistry in mice. Phenotypic analysis and microarray analysis of the thymus were conducted in wild and AQP11 null mice at 21 day old before the development of uremia due to polycystic kidneys. Results Both mRNA and protein expressions of AQP11 in the thymus were higher than those of the kidney by RT-PCR and Western blot. Interestingly, the size of the thymus from AQP11 null mice was smaller than that of the wild mice: 28mg vs. 56mg, even after the correction by the body weight: 0.59 % vs. 0.82 %, as the body size of AQP11 null mice was smaller. The vacuolated medullary epithelial cells were observed in the thymus of AQP11 null mice with narrowed cortex. The immunohistochemical analysis revealed the AQP11 expression at the stromal-epithelial cells in the thymus. The microarray analysis of the gene expression in the thymus was compared between the wild and AQP11 null thymus by the annotation analysis based on the David Bioinformatics Resources 6.8 (beta). The up-regulated 66 genes more than 1.5 folds in AQP11 null thymus are mainly related to the PI3K/Akt signaling pathway to promote metabolism, proliferation, cell survival, growth and angiogenesis. On the other hand, the down-regulated 55 genes less than half in AQP11 null thymus are related to energy production and the peroxisome proliferator-activated receptor (PPAR) signaling pathway. Further RT-qPCR analysis confirmed the enhanced expression of Egfr (Epidermal Growth Factor Receptor), Itgb4 (Integrin beta-4) and Il2ra (interleukin 2 receptor alpha) and the diminished expression of aquaglyceroporin AQP7, Pck1 (Phosphoenolpyruvate carboxy-kinase 1) and Ucp1 (Mitochondrial uncoupling protein 1). The up-regulated genes for growth signaling was unexpected with the smaller thymus but may support the survival of the hypoplastic thymus as a compensation. On the other hand, the down-regulated genes may compromise fat-glucose-energy metabolism in the thymus leading to the smaller thymus, which may be aggravated by the decreased expression of AQP7, another glycerol channel. Conclusion AQP11 may play an important role in the metabolic control of the developing thymus possibly through its glycerol transport. The smaller thymus with narrower cortex in AQP11 null mice may lead to immunological dysfunction, which is currently under study.

High Heels sebagai Agensi bagi Tubuh Wanita

2020 ◽  
Vol 5 (2) ◽  
pp. 196
Author(s):  
Sunandar Macpal ◽  
Fathianabilla Azhar
Keyword(s):  
Body Image ◽  
Literature Review ◽  
The Body ◽  
The Other ◽  
Body Image Disturbance ◽  
Other Hand ◽  
High Heels ◽  
Experience Pain ◽  
Woman's Body

The aims of this paper is to explain the use of high heels as an agency for a woman's body. Agency context refers to pain in the body but pain is perceived as something positive. In this paper, the method used is a literature review by reviewing writings related to the use of high heels. The findings in this paper that women experience body image disturbance or anxiety because they feel themselves are not beautiful or not attractive. The use of high heels, makes women more attractive and more confident, on the other hand the use of high heels actually makes women feel pain and discomfort. However, for the achievement of beauty standards, women voluntarily allow their bodies to experience pain. However, the agency's willingness to beauty standards here is meaningless without filtering and directly accepted. Instead women keep negotiating with themselves so as to make a decision why use high heels.

Basic Concepts and Definitions of Allergology

2019 ◽  
Author(s):  
Zoran Vrucinic
Keyword(s):  
Immune Reaction ◽  
The Body ◽  
The Other ◽  
Specific Response ◽  
Other Hand ◽  
Medical Vocabulary ◽  
The Future ◽  
Basic Concepts ◽  
Basic Insight ◽  
Insight Into

The future of medicine belongs to immunology and alergology. I tried to not be too wide in description, but on the other hand to mention the most important concepts of alergology to make access to these diseases more understandable, logical and more useful for our patients, that without complex pathophysiology and mechanism of immune reaction,we gain some basic insight into immunological principles. The name allergy to medicine was introduced by Pirquet in 1906, and is of Greek origin (allos-other + ergon-act; different reaction), essentially representing the reaction of an organism to a substance that has already been in contact with it, and manifested as a specific response thatmanifests as either a heightened reaction, a hypersensitivity, or as a reduced reaction immunity. Synonyms for hypersensitivity are: altered reactivity, reaction, hypersensitivity. The word sensitization comes from the Latin (sensibilitas, atis, f.), which means sensibility,sensitivity, and has retained that meaning in medical vocabulary, while in immunology and allergology this term implies the creation of hypersensitivity to an antigen. Antigen comes from the Greek words, anti-anti + genos-genus, the opposite, anti-substance substance that causes the body to produce antibodies.

scholarly journals Towards a Kenotic Identity Politics: Migration, Transformation and the Eucharist

Religions ◽  
2021 ◽  
Vol 12 (6) ◽  
pp. 380
Author(s):  
Matthew John Paul Tan
Keyword(s):  
Identity Politics ◽  
The Body ◽  
The Other ◽  
Body Of Christ ◽  
Eucharistic Theology ◽  
Critical Tradition ◽  
Other Hand ◽  
Massive Influx ◽  
Identity Based ◽  
The One

This paper will focus on one element of the pushback against the massive influx of immigrants taken in for humanitarian purposes, namely, an identity-based chauvinism which uses identity as the point of resistance to the perceived dilution of that identity, brought about by the transformation of culture induced by the incorporation of a foreign other. The solution to this perceived dilution is a simultaneous defence of that culture and a demand for a conformity to it. While those in the critical tradition have encouraged a counter-position of revolutionary transformation by the other through ethics, dialogue, or the multitude, such a transformation is arguably impeded by what is ultimately a repetition of the metaphysics of conformity. Drawing on the personalism of Emmanuel Mounier and the Eucharistic theology of Creston Davis and Aaron Riches, this paper submits an alternative identity politics position that completes the revolutionary impulse. Identity here is not the flashpoint of a self-serving conflict, but the launch-point of politics of self-emptying, whose hallmarks include, on the one hand, a never-ending reception of transformation by the other, and on the other hand, an anchoring in the Body of Christ that is at once ever-changing and never-changing.

scholarly journals Decreased ligand affinity rather than glucocorticoid receptor down-regulation in patients with endogenous Cushing's syndrome

2000 ◽  
pp. 472-476 ◽  
Author(s):  
NA Huizenga ◽  
WW De Herder ◽  
JW Koper ◽  
P de Lange ◽  
D AJ v Lely ◽  
...  
Keyword(s):  
Glucocorticoid Receptor ◽  
Cushing’S Syndrome ◽  
Cushing's Syndrome ◽  
The Body ◽  
The Other ◽  
Down Regulation ◽  
Ligand Affinity ◽  
Other Hand ◽  
Receptor Number ◽  
Cortisol Levels

OBJECTIVE: Glucocorticoids (GCs) serve a variety of important functions throughout the body. The synthesis and secretion of GCs are under the strict influence of the hypothalamo-pituitary-adrenal axis. The mechanisms of action of GCs are mediated by the intracellular glucocorticoid receptor (GR). Over the years, many studies have been performed concerning the regulation of GR expression by GC concentrations. METHODS: In the present study, we determined the characteristics of the GR in peripheral mononuclear blood leukocytes (PBML) from thirteen patients with endogenous Cushing's syndrome and fifteen control subjects, using a whole cell dexamethasone binding assay. Furthermore, cortisol concentrations were determined in order to investigate a possible relationship between serum cortisol levels and receptor characteristics. RESULTS: There were no differences in mean receptor number between patients and controls. On the other hand, a significantly lower ligand affinity was identified in cells from patients with Cushing's syndrome compared with controls. A complete normalisation of the ligand affinity was observed after treatment in the only patient tested in this respect, whereas the receptor number was not affected. In patients, there was a statistically significant negative correlation between cortisol concentrations and ligand affinity, which was not found in controls. CONCLUSION: Receptor down-regulation does not occur in PBML from patients with endogenous Cushing's syndrome. On the other hand, there seems to be a diminished ligand affinity which possibly reflects receptor modification in response to exposure to the continuously high cortisol levels in patients with Cushing's syndrome. This assumption is substantiated by the fact that in one patient a normalisation of the ligand affinity after complete remission of the disease was seen.

Electrical interactions between the giant axons of a polychaete worm (Sabella penicillus L.)

1980 ◽  
Vol 84 (1) ◽  
pp. 119-136
Author(s):  
D. Mellon ◽  
J. E. Treherne ◽  
N. J. Lane ◽  
J. B. Harrison ◽  
C. K. Langley
Keyword(s):  
Safety Factor ◽  
Nerve Cord ◽  
Action Potentials ◽  
Divalent Cations ◽  
Muscular Contraction ◽  
The Body ◽  
The Other ◽  
Intracellular Recordings ◽  
Giant Axons ◽  
Other Hand

Intracellular recordings demonstrated a transfer of impulses between the paired giant axons of Sabella, apparently along narrow axonal processes contained within the paired commissures which link the nerve cords in each segment of the body. This transfer appears not to be achieved by chemical transmission, as has been previously supposed. This is indicated by the spread of depolarizing and hyperpolarizing voltage changes between the giant axons, the lack of effects of changes in the concentrations of external divalent cations on impulse transmission and by the effects of hyperpolarization in reducing the amplitude of the depolarizing potential which precedes the action potentials in the follower axon. The ten-to-one attenuation of electronic potentials between the giant axons argues against the possibility of an exclusively passive spread of potential along the axonal processes which link the axons. Observation of impulse traffic within the nerve cord commissures indicates, on the other hand, that transmission is achieved by conduction of action potentials along the axonal processes which link the giant axons. At least four pairs of intact commissures are necessary for inter-axonal transmission, the overall density of current injected at multiple sites on the follower axon being, it is presumed, sufficient to overcome the reduction in safety factor imposed by the geometry of the system in the region where axonal processes join the giant axons. The segmental transmission between the giant axons ensures effective synchronization of impulse traffic initiated in any region of the body and, thus, co-ordination of muscular contraction, during rapid withdrawal responses of the worm.

A Liberating Experience. On Becoming a Work of Art

2020 ◽  
Vol 7 (2) ◽  
pp. 411-430
Author(s):  
Maja Tabea Jerrentrup
Keyword(s):  
Three Dimensional ◽  
The Body ◽  
The Other ◽  
Work Of Art ◽  
Other Hand ◽  
Film Clips ◽  
Long Time ◽  
The One ◽  
Body Painting ◽  
Dimensional Surface

Abstract The art of bodypainting that is fairly unknown to a wider public turns the body into a canvas - it is a frequently used phrase in the field of bodypainting that illustrates the challenge it faces: it uses a three-dimensional surface and has to cope with its irregularities, but also with the model’s abilities and characteristics. This paper looks at individuals who are turned into art by bodypainting. Although body painting can be very challenging for them - they have to expose their bodies and to stand still for a long time while getting transformed - models report that they enjoy both the process and the result, even if they are not confident about their own bodies. Among the reasons there are physical aspects like the sensual enjoyment, but also the feeling of being part of something artistic. This is enhanced and preserved through double staging - becoming a threedimentional work of art and then being staged for photography or film clips. This process gives the model the chance to experience their own body in a detached way. On the one hand, bodypainting closely relates to the body and on the other hand, it can help to over-come the body.

On the Measurement of the Palate in Idiots and Imbeciles

1876 ◽  
Vol 22 (98) ◽  
pp. 196-201 ◽  
Author(s):  
T. Claye Shaw
Keyword(s):  
Prima Facie ◽  
The Body ◽  
The Other ◽  
General Idea ◽  
Cranial Sutures ◽  
High Arched Palate ◽  
Other Hand ◽  
The Face

There is a general idea expressed in text-books, and more or less freely asserted in practice, but which I shall prove to be a fallacy, that a high-arched palate is so frequently met with in idiocy and imbecility that it may be taken as a sign of their existence. Indeed, when a case of this kind is brought forward the patient is made to open his mouth, under the conviction that a high palate will be found as certainly as a superficial alteration of the tongue in gastric disturbance. We shall see that the connection is an accidental one; and there is, in reality, no relationship between the development of the intellect and the height and width of the palate. If we consider that the bones of the cranium are developed in a different manner from those of the face, and that ossification at the base is complete long before that of the bones forming the palate, it is clear that there can be no primâ facie reason for thinking that because a person has an imperfect brain he should therefore have an imperfect palate; yet such an interdependence is held. It is quite true that a constitutional taint, such as rickets or syphilis, which affects the ossification of the bones generally and the cranial sutures, would probably affect the palatine bones, and hence it is that many idiots and imbeciles are found to have high or imperfect palates: but on the other hand some modifying taint may dwarf the height of the body, may affect the shape of the head to such an extent as to make an idiot of the microcephalic type, and yet leave the palate untouched, perfect in all conditions of width, height, number, quality, and regularity of teeth.

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