In vitro sensitization of thymocytes. Role of H-21 I region determinants and cell-free mixed leukocyte culture supernates in generation of cytotoxic responses.
1978 ◽
Vol 148
(4)
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pp. 953-962
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Keyword(s):
Stimulation of thymocytes in vitro by spleen cells differing for the entire H-2 complex leads to a significant proliferative response without a significant cell-mediated lympholysis (CML) response. Addition of soluble cell-free supernates (SF), (taken from a 7-day mixed leukocyte culture) enables these cultures to develop CML response. For optimal CML response, the SF has to be added within 48 h of onset of cultures. Although with spleen cells as responding cells, SF could quantitatively replace I-region different stimulating cells for generation of CML responses, with thymocytes as responding cells, stimulation with I-region cells appeared obligatory for the generation of CML responses. The implications of these findings are discussed.
1971 ◽
Vol 133
(5)
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pp. 963-972
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Keyword(s):
1982 ◽
Vol 18
(9)
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pp. 875-883
Keyword(s):
1971 ◽
Vol 134
(2)
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pp. 395-416
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Keyword(s):
1989 ◽
Vol 66
(4)
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pp. 1547-1552
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Keyword(s):
1985 ◽
Vol 78
(2)
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pp. 227-237
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Keyword(s):
Keyword(s):