scholarly journals CELLULAR MECHANISMS OF PROTEIN METABOLISM IN THE NEPHRON

1954 ◽  
Vol 99 (6) ◽  
pp. 637-646 ◽  
Author(s):  
N. Kretchmer ◽  
Francis J. Cherot
Keyword(s):  
Amino Acid ◽  
Protein Metabolism ◽  
Free Amino ◽  
Renal Cortex ◽  
Concentration Level ◽  
Amino Nitrogen ◽  
Maximal Concentration ◽  
Cellular Mechanisms ◽  
Tubule Cells

Parenteral injection of amino acids resulted in the formation of Gram-positive droplets in the cytoplasm of the proximal convoluted tubule cells of the kidney of the rat within 15 minutes after intravenous administration. At this time the free alpha amino nitrogen in the cortex of the kidney had increased 2-fold. At the end of 1 hour this level was still somewhat higher than that of the control animals. The administration of increasing amounts of the amino acid disclosed the existence of a maximal concentration level in the renal cortex. When it was reached droplets appeared. Fractionation of the cells 15 minutes after the injection of lysine resulted in the recovery of free amino acid in the supernatant fluid but 1 hour after the injection lysine was contained within the particulate protein of the fractions which contain droplets. The increase in lysine was of the order of 2- 3-fold. It is concluded that the mitochondria-droplet complex is a locus of amino acid concentration and metabolism within the cytoplasm of the renal cell. A method is described for the determination of lysine in micro quantities.

Van Slyke Manometric Apparatus Modified for Determination of Free Amino Nitrogen in Solid Samples

1956 ◽  
Vol 28 (1) ◽  
pp. 144-144 ◽  
Author(s):  
K. T. Williams ◽  
M. C. Long
Keyword(s):  
Free Amino ◽  
Amino Nitrogen ◽  
Solid Samples

scholarly journals Determination of free amino acids in plants by liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS)

2015 ◽  
Vol 7 (18) ◽  
pp. 7574-7581 ◽  
Author(s):  
Magdalena M. Dziągwa-Becker ◽  
Jose M. Marin Ramos ◽  
Jakub K. Topolski ◽  
Wiesław A. Oleszek
Keyword(s):  
Mass Spectrometry ◽  
Amino Acids ◽  
Liquid Chromatography ◽  
Amino Acid ◽  
Tandem Mass Spectrometry ◽  
Free Amino ◽  
Tandem Mass ◽  
Amino Acid Determination ◽  
Acid Determination

Free amino acid determination in plants by LC-MS/MS.

Simultaneous Determination of S-Methylmethionine, Vitamin U and Free Amino Acids in Extracts of Green Tea with an HPLC-Amino Acid Analyzer

1987 ◽  
Vol 51 (9) ◽  
pp. 2479-2484
Author(s):  
Kozo Ohtsuki ◽  
Makoto Kawabata ◽  
Hiroshi Kokura ◽  
Kuniko Taguchi
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Simultaneous Determination ◽  
Green Tea ◽  
Free Amino Acids ◽  
Free Amino ◽  
Amino Acid Analyzer ◽  
Vitamin U

scholarly journals Determination of Free Amino Acid Nitrogen in the Blood Plasma by Colorimetric Method with Ninhydrin

1981 ◽  
Vol 52 (2) ◽  
pp. 136-138
Author(s):  
Ichiro HAGIMORI ◽  
Makoto KANDATSU
Keyword(s):  
Amino Acid ◽  
Blood Plasma ◽  
Free Amino Acid ◽  
Free Amino ◽  
Colorimetric Method ◽  
Amino Acid Nitrogen

scholarly journals The effect of a high dose of 3-hydroxy-3-methylbutyrate on protein metabolism in growing lambs

1997 ◽  
Vol 77 (6) ◽  
pp. 885-896 ◽  
Author(s):  
Isabelle Papet ◽  
Piotr Ostaszewski ◽  
Francoise Glomot ◽  
Christiane Obled ◽  
Magali Faure ◽  
...  
Keyword(s):  
Protein Synthesis ◽  
Amino Acid ◽  
Free Amino Acid ◽  
Protein Metabolism ◽  
Free Amino ◽  
Skeletal Muscles ◽  
Whole Body ◽  
Control Group ◽  
High Dose ◽  
Body Protein

AbstractThe effect of a high dose of 3-hydroxy-3-methylbutyrate (HMB, a leucine catabolite) on protein metabolism was investigated in growing male lambs fed on hay and concentrate. Concentrate was supplemented with either Ca(HMB)2 (4g/kg) or Ca(C03)2 in experimental (HMB) and control groups respectively. Both groups consisted of six 2-month old lambs. Three complementary methods to study protein metabolism were carried out consecutively 2·5 months after beginning the dietary treatment: whole body phenylalanine fluxes, postprandial plasma free amino acid time course and fractional rates of protein synthesis in skeletal muscles. Feeding a high dose of HMB led to a significant increase in some plasma free amino acids compared with controls. Total, oxidative and non-oxidative phenylalanine fluxes were not modified by dietary HMB supplementation. Similarly, an acute infusion of HMB, in the control group, did not change these fluxes. In skeletal muscles, fractional rates of protein synthesis were not affected by long-term dietary supplementation with HMB. Taken together our results showed that administration of a high dose of HMB to lambs was able to modify plasma free amino acid pattern without any effect on whole-body protein turnover and skeletal muscle protein synthesis

Research on Quality Variety of the Commercially Available Yogurt during Storage

2013 ◽  
Vol 864-867 ◽  
pp. 567-570
Author(s):  
Ling Shuai Meng ◽  
Jun Rui Wu ◽  
Shuang Gao ◽  
Xin Xu ◽  
Ri Na Wu
Keyword(s):  
Lactic Acid ◽  
Amino Acid ◽  
Free Amino ◽  
Mass Concentration ◽  
Nitrogen Concentration ◽  
Amino Nitrogen ◽  
Viable Count ◽  
National Standards ◽  
In The Beginning ◽  
Measuring Ph

By measuring pH, acidity, mass concentration of free amino nitrogen and viable count of lactic acid bacteria from yogurt under refrigerated conditions, to study changes in the indicators of yogurt during the shelf. The results showed that during the yogurt shelf life, pH decreased, acidity increased, viable count of Lactobacillus was on the decline, nitrogen concentration of free amino acid increased less in the beginning of the refrigeration, but the indicators were still in line with national standards.

scholarly journals Whole body leucine flux in HIV-infected patients treated with or without protease inhibitors

2006 ◽  
Vol 290 (4) ◽  
pp. E685-E693 ◽  
Author(s):  
Magali Prod’homme ◽  
Cécile Rochon ◽  
Michèle Balage ◽  
Henri Laurichesse ◽  
Igor Tauveron ◽  
...  
Keyword(s):  
Amino Acid ◽  
Protein Metabolism ◽  
Glucose Infusion ◽  
Fat Free Mass ◽  
Whole Body ◽  
Constant Infusion ◽  
Body Protein ◽  
X Ray ◽  
Immunodeficiency Virus

The present study was carried out to assess the effects of protease inhibitor (PI) therapy on basal whole body protein metabolism and its response to acute amino acid-glucose infusion in 14 human immunodeficiency virus (HIV)-infected patients. Patients treated with PIs (PI+, 7 patients) or without PIs (PI−, 7 patients) were studied after an overnight fast during a 180-min basal period followed by a 140-min period of amino acid-glucose infusion. Protein metabolism was investigated by a primed constant infusion of l-[1-13C]leucine. Dual-energy X-ray absorptiometry for determination of fat-free mass (FFM) and body fat mass measured body composition. In the postabsorptive state, whole body leucine balance was 2.5 times ( P < 0.05) less negative in the PI+ than in the PI− group. In HIV-infected patients treated with PIs, the oxidative leucine disposal during an acute amino acid-glucose infusion was lower (0.58 ± 0.09 vs. 0.81 ± 0.07 μmol·kg FFM−1·min−1 using plasma [13C]leucine enrichment, P = 0.06; or 0.70 ± 0.10 vs. 0.99 ± 0.08 μmol·kg FFM−1·min−1 using plasma [13C]ketoisocaproic acid enrichment, P = 0.04 in PI+ and PI− groups, respectively) than in patients treated without PIs. Consequently, whole body nonoxidative leucine disposal (an index of protein synthesis) and leucine balance (0.50 ± 0.10 vs. 0.18 ± 0.06 μmol·kg FFM·−1·min−1 in PI+ and PI− groups respectively, P < 0.05) were significantly improved during amino acid-glucose infusion in patients treated with PIs. However, whereas the response of whole body protein anabolism to an amino acid-glucose infusion was increased in HIV-infected patients treated with PIs, any improvement in lean body mass was detected.

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