scholarly journals Stabilization of a prokaryotic LAT transporter by random mutagenesis

2016 ◽  
Vol 147 (4) ◽  
pp. 353-368 ◽  
Author(s):  
Arturo Rodríguez-Banqueri ◽  
Ekaitz Errasti-Murugarren ◽  
Paola Bartoccioni ◽  
Lukasz Kowalczyk ◽  
Alex Perálvarez-Marín ◽  
...  
Keyword(s):  
Three Dimensional ◽  
Random Mutagenesis ◽  
Membrane Insertion ◽  
Structural Determination ◽  
New Approach ◽  
Membrane Transport Proteins ◽  
Engineer Stability ◽  
The Stability ◽  
Optimal Candidate ◽  
Detergent Micelles

The knowledge of three-dimensional structures at atomic resolution of membrane transport proteins has improved considerably our understanding of their physiological roles and pathological implications. However, most structural biology techniques require an optimal candidate within a protein family for structural determination with (a) reasonable production in heterologous hosts and (b) good stability in detergent micelles. SteT, the Bacillus subtilis l-serine/l-threonine exchanger is the best-known prokaryotic paradigm of the mammalian l–amino acid transporter (LAT) family. Unfortunately, SteT’s lousy stability after extracting from the membrane prevents its structural characterization. Here, we have used an approach based on random mutagenesis to engineer stability in SteT. Using a split GFP complementation assay as reporter of protein expression and membrane insertion, we created a library of 70 SteT mutants each containing random replacements of one or two residues situated in the transmembrane domains. Analysis of expression and monodispersity in detergent of this library permitted the identification of evolved versions of SteT with a significant increase in both expression yield and stability in detergent with respect to wild type. In addition, these experiments revealed a correlation between the yield of expression and the stability in detergent micelles. Finally, and based on protein delipidation and relipidation assays together with transport experiments, possible mechanisms of SteT stabilization are discussed. Besides optimizing a member of the LAT family for structural determination, our work proposes a new approach that can be used to optimize any membrane protein of interest.

scholarly journals A Simple Analytical Model for Estimating the Dissolution-Driven Instability in a Porous Medium

Fluids ◽  
2018 ◽  
Vol 3 (3) ◽  
pp. 60
Author(s):  
Layachi Hadji
Keyword(s):  
Stability Problem ◽  
Three Dimensional ◽  
Step Function ◽  
New Approach ◽  
Engineering Mathematics ◽  
Alternative Approach ◽  
Function Base ◽  
The Stability ◽  
Rayleigh Benard ◽  
Profile Approach

This article deals with the stability problem that arises in the modeling of the geological sequestration of carbon dioxide. It provides a more detailed description of the alternative approach to tackling the stability problem put forth by Vo and Hadji (Physics of Fluids, 2017, 29, 127101) and Wanstall and Hadji (Journal of Engineering Mathematics, 2018, 108, 53–71), and it extends two-dimensional analysis to the three-dimensional case. This new approach, which is based on a step-function base profile, is contrasted with the usual time-evolving base state. While both provide only estimates for the instability threshold values, the step-function base profile approach has one great advantage in the sense that the problem at hand can be viewed as a stationary Rayleigh–Bénard problem, the model of which is physically sound and the stability of which is not only well-defined but can be analyzed by a variety of existing analytical methods using only paper and pencil.

Manipulation with a polyarticulated mechanical hand: a new efficient real-time method for computing fingertip forces for a global manipulation strategy

Robotica ◽  
2005 ◽  
Vol 23 (4) ◽  
pp. 479-490 ◽  
Author(s):  
J. P. Gazeau ◽  
S. Zeghloul ◽  
G. Ramirez
Keyword(s):  
Optimization Problem ◽  
Three Dimensional ◽  
Inequality Constraints ◽  
Minimal Distance ◽  
Linear Quadratic ◽  
New Approach ◽  
Three Dimensional Objects ◽  
Mechanical Hand ◽  
The Stability ◽  
Manipulation Strategy

This paper proposes an efficient algorithm for computing finger forces involved in a three-dimensional objects grasp. Effective finger force computation is necessary for the successful manipulation on an object by a multifingered robot hand. Based on previous works, the stability forces are computed as a solution of an optimization problem. This optimization problem is mapped into a linear quadratic problem under inequality constraints. We propose a new approach for this problem: the problem is solved as a minimal distance calculation problem in the forces space. The results obtained by simulation demonstrate the efficiency and the numerical stability of the method. This method is used with the LMS mechanical hand as a component of the global control strategy dedicated to the object manipulation.

A Liquid Metal Flow Between Two Coaxial Cylinders System

2019 ◽  
Vol 11 (1) ◽  
pp. 79-86
Author(s):  
Abdelkrim Merah ◽  
Ridha Kelaiaia ◽  
Faiza Mokhtari
Keyword(s):  
Couette Flow ◽  
Metal Flow ◽  
Three Dimensional ◽  
Liquid Sodium ◽  
Taylor Vortex ◽  
Turbulent Regime ◽  
Coaxial Cylinders ◽  
Concentric Cylinders ◽  
Ideal Tool ◽  
The Stability

Abstract The Taylor-Couette flow between two rotating coaxial cylinders remains an ideal tool for understanding the mechanism of the transition from laminar to turbulent regime in rotating flow for the scientific community. We present for different Taylor numbers a set of three-dimensional numerical investigations of the stability and transition from Couette flow to Taylor vortex regime of a viscous incompressible fluid (liquid sodium) between two concentric cylinders with the inner one rotating and the outer one at rest. We seek the onset of the first instability and we compare the obtained results for different velocity rates. We calculate the corresponding Taylor number in order to show its effect on flow patterns and pressure field.

Host-Guest Interactions on Electrode Surfaces for Immobilization of Molecular Catalysts

2020 ◽  
Author(s):  
Laurent Sévery ◽  
Jacek Szczerbiński ◽  
Mert Taskin ◽  
Isik Tuncay ◽  
Fernanda Brandalise Nunes ◽  
...  
Keyword(s):  
Aqueous Media ◽  
Heterogeneous Systems ◽  
Catalytic Systems ◽  
New Approach ◽  
Molecular Systems ◽  
Electrode Surfaces ◽  
Catalytic Surfaces ◽  
Oxide Electrodes ◽  
The Stability ◽  
Molecular Catalysts

The strategy of anchoring molecular catalysts on electrode surfaces combines the high selectivity and activity of molecular systems with the practicality of heterogeneous systems. The stability of molecular catalysts is, however, far less than that of traditional heterogeneous electrocatalysts, and therefore a method to easily replace anchored molecular catalysts that have degraded could make such electrosynthetic systems more attractive. Here, we apply a non-covalent “click” chemistry approach to reversibly bind molecular electrocatalysts to electrode surfaces via host-guest complexation with surface-anchored cyclodextrins. The host-guest interaction is remarkably strong and allows the flow of electrons between the electrode and the guest catalyst. Electrosynthesis in both organic and aqueous media was demonstrated on metal oxide electrodes, with stability on the order of hours. The catalytic surfaces can be recycled by controlled release of the guest from the host cavities and readsorption of fresh guest. This strategy represents a new approach to practical molecular-based catalytic systems.

Handheld Laser Scanner Research

2019 ◽  
Vol 952 (10) ◽  
pp. 47-54
Author(s):  
A.V. Komissarov ◽  
A.V. Remizov ◽  
M.M. Shlyakhova ◽  
K.K. Yambaev
Keyword(s):  
Point Cloud ◽  
Three Dimensional ◽  
Laser Scanner ◽  
Test Site ◽  
Optical Systems ◽  
Advantages And Disadvantages ◽  
Site Survey ◽  
Laser Scanners ◽  
Three Dimensional Models ◽  
The Stability

The authors consider hand-held laser scanners, as a new photogrammetric tool for obtaining three-dimensional models of objects. The principle of their work and the newest optical systems based on various sensors measuring the depth of space are described in detail. The method of simultaneous navigation and mapping (SLAM) used for combining single scans into point cloud is outlined. The formulated tasks and methods for performing studies of the DotProduct (USA) hand-held laser scanner DPI?8X based on a test site survey are presented. The accuracy requirements for determining the coordinates of polygon points are given. The essence of the performed experimental research of the DPI?8X scanner is described, including scanning of a test object at various scanner distances, shooting a test polygon from various scanner positions and building point cloud, repeatedly shooting the same area of the polygon to check the stability of the scanner. The data on the assessment of accuracy and analysis of research results are given. Fields of applying hand-held laser scanners, their advantages and disadvantages are identified.

scholarly journals Gum Tragacanth (GT): A Versatile Biocompatible Material beyond Borders

Molecules ◽  
2021 ◽  
Vol 26 (6) ◽  
pp. 1510 ◽  
Author(s):  
Mohammad Ehsan Taghavizadeh Yazdi ◽  
Simin Nazarnezhad ◽  
Seyed Hadi Mousavi ◽  
Mohammad Sadegh Amiri ◽  
Majid Darroudi ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Food Packaging ◽  
Three Dimensional ◽  
Great Promise ◽  
Anionic Polymer ◽  
Gum Tragacanth ◽  
New Horizons ◽  
Tissue Healing ◽  
The Stability ◽  
Therapeutic Substance

The use of naturally occurring materials in biomedicine has been increasingly attracting the researchers’ interest and, in this regard, gum tragacanth (GT) is recently showing great promise as a therapeutic substance in tissue engineering and regenerative medicine. As a polysaccharide, GT can be easily extracted from the stems and branches of various species of Astragalus. This anionic polymer is known to be a biodegradable, non-allergenic, non-toxic, and non-carcinogenic material. The stability against microbial, heat and acid degradation has made GT an attractive material not only in industrial settings (e.g., food packaging) but also in biomedical approaches (e.g., drug delivery). Over time, GT has been shown to be a useful reagent in the formation and stabilization of metal nanoparticles in the context of green chemistry. With the advent of tissue engineering, GT has also been utilized for the fabrication of three-dimensional (3D) scaffolds applied for both hard and soft tissue healing strategies. However, more research is needed for defining GT applicability in the future of biomedical engineering. On this object, the present review aims to provide a state-of-the-art overview of GT in biomedicine and tries to open new horizons in the field based on its inherent characteristics.

scholarly journals Exploration of collagen cavitation based on peptide electrolysis

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Rui Zhai ◽  
Hui Chen ◽  
Zhihua Shan
Keyword(s):  
Structural Changes ◽  
Preparation Method ◽  
Supporting Electrolyte ◽  
Three Dimensional ◽  
Anode Region ◽  
New Approach ◽  
New Material ◽  
Animal Skin ◽  
Exchange Membrane ◽  
Material Preparation

AbstractElectrochemical modification of animal skin is a new material preparation method and new direction of research exploration. In this study, under the action of the electric field using NaCl as the supporting electrolyte, the effect of electrolysis on Glycyl-glycine(GlyGl), gelatin(Gel) and Three-dimensional rawhide collagen(3DC) were determined. The amino group of GlyGl is quickly eliminated within the anode region by electrolysis isolated by an anion exchange membrane. Using the same method, it was found that the molecular weight of Gel and the isoelectric point of the Gel decreased, and the viscosity and transparency of the Gel solution obviously changed. The electrolytic dissolution and structural changes of 3DC were further investigated. The results of TOC and TN showed that the organic matter in 3DC was dissolved by electrolysis, and the tissue cavitation was obvious. A new approach for the preparation of collagen-based multi-pore biomaterials by electrochemical method was explored.

scholarly journals Methodology and Models for Individuals’ Creditworthiness Management Using Digital Footprint Data and Machine Learning Methods

Mathematics ◽  
2021 ◽  
Vol 9 (15) ◽  
pp. 1820
Author(s):  
Ekaterina V. Orlova
Keyword(s):  
Interest Rates ◽  
Additional Data ◽  
Risk Level ◽  
Gradient Boosting ◽  
Cluster Number ◽  
New Approach ◽  
Credit Risks ◽  
Stochastic Gradient Boosting ◽  
The Stability ◽  
Digital Footprints

This research deals with the challenge of reducing banks’ credit risks associated with the insolvency of borrowing individuals. To solve this challenge, we propose a new approach, methodology and models for assessing individual creditworthiness, with additional data about borrowers’ digital footprints to implement comprehensive analysis and prediction of a borrower’s credit profile. We suggest a model for borrowers’ clustering based on the method of hierarchical clustering and the k-means method, which groups actual borrowers having similar creditworthiness and similar credit risks into homogeneous clusters. We also design the model for borrowers’ classification based on the stochastic gradient boosting (SGB) method, which reliably determines the cluster number and therefore the risk level for a new borrower. The developed models are the basis for decision making regarding the decision about lending value, interest rates and lending terms for each risk-homogeneous borrower’s group. The modified version of the methodology for assessing individual creditworthiness is presented, which is to reduce the credit risks and to increase the stability and profitability of financial organizations.

scholarly journals Emergent chiral symmetry in a three-dimensional interacting Dirac liquid

2021 ◽  
Vol 2021 (1) ◽  
Author(s):  
András L. Szabó ◽  
Bitan Roy
Keyword(s):  
Fixed Points ◽  
Chiral Symmetry ◽  
Rotational Symmetry ◽  
Three Dimensional ◽  
Spatial Dimension ◽  
Dirac Fermions ◽  
Electronic Interactions ◽  
Emergent Phenomena ◽  
Ordered Phases ◽  
The Stability

Abstract We compute the effects of strong Hubbardlike local electronic interactions on three-dimensional four-component massless Dirac fermions, which in a noninteracting system possess a microscopic global U(1) ⊗ SU(2) chiral symmetry. A concrete lattice realization of such chiral Dirac excitations is presented, and the role of electron-electron interactions is studied by performing a field theoretic renormalization group (RG) analysis, controlled by a small parameter ϵ with ϵ = d−1, about the lower-critical one spatial dimension. Besides the noninteracting Gaussian fixed point, the system supports four quantum critical and four bicritical points at nonvanishing interaction couplings ∼ ϵ. Even though the chiral symmetry is absent in the interacting model, it gets restored (either partially or fully) at various RG fixed points as emergent phenomena. A representative cut of the global phase diagram displays a confluence of scalar and pseudoscalar excitonic and superconducting (such as the s-wave and p-wave) mass ordered phases, manifesting restoration of (a) chiral U(1) symmetry between two excitonic masses for repulsive interactions and (b) pseudospin SU(2) symmetry between scalar or pseudoscalar excitonic and superconducting masses for attractive interactions. Finally, we perturbatively study the effects of weak rotational symmetry breaking on the stability of various RG fixed points.

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