Assessment of the Activity of a Novel Light-Activated Antimicrobial Coating in a Clinical Environment

2008 ◽  
Vol 29 (12) ◽  
pp. 1181-1184 ◽  
Author(s):  
Valérie Decraene ◽  
Jonathan Pratten ◽  
Michael Wilson
Keyword(s):  
Cellulose Acetate ◽  
Toluidine Blue ◽  
Rose Bengal ◽  
Antimicrobial Agents ◽  
Microbial Load ◽  
Clinical Environment ◽  
Antimicrobial Coating

Cellulose acetate coatings containing the light-activated antimicrobial agents toluidine blue O and rose bengal have previously been shown to be successful in killing a range of microorganisms. Here, we report on the ability of these coatings to achieve reductions in the microbial load on surfaces in a clinical environment.

scholarly journals Cellulose Acetate Containing Toluidine Blue and Rose Bengal Is an Effective Antimicrobial Coating when Exposed to White Light

2006 ◽  
Vol 72 (6) ◽  
pp. 4436-4439 ◽  
Author(s):  
Val�rie Decraene ◽  
Jonathan Pratten ◽  
Michael Wilson
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Candida Albicans ◽  
Clostridium Difficile ◽  
Cellulose Acetate ◽  
Toluidine Blue ◽  
White Light ◽  
Rose Bengal ◽  
Microbial Load ◽  
Hospital Associated Infections

ABSTRACT Simple methods of reducing the microbial load on surfaces in hospitals are needed to reduce the risk of hospital-associated infections. Here we report on the ability of a cellulose acetate coating containing the photosensitizers toluidine blue and rose bengal to kill microbes (Staphylococcus aureus, Escherichia coli, Clostridium difficile, a bacteriophage, and Candida albicans) on its surface when illuminated with white light.

scholarly journals Efficacy of a Novel Light-Activated Antimicrobial Coating for Disinfecting Hospital Surfaces

2011 ◽  
Vol 32 (11) ◽  
pp. 1130-1132 ◽  
Author(s):  
Salim Ismail ◽  
Stefano Perni ◽  
Jonathan Pratten ◽  
Ivan Parkin ◽  
Michael Wilson
Keyword(s):  
Methylene Blue ◽  
Gold Nanoparticles ◽  
Antimicrobial Agent ◽  
White Light ◽  
Microbial Load ◽  
Clinical Environment ◽  
Antimicrobial Coating ◽  
Silicone Polymers

Silicone polymers containing the light-activated antimicrobial agent methylene blue with or without gold nanoparticles were evaluated for their ability to reduce the microbial load on surfaces in a clinical environment. When irradiated with white light, polymers containing nanogold were more effective in this respect than those containing only methylene blue.

Antimicrobial Coating of Devices for Prevention of Infection: Principles and Protection

2007 ◽  
Vol 30 (9) ◽  
pp. 820-827 ◽  
Author(s):  
R. O. Darouiche
Keyword(s):  
Antimicrobial Agents ◽  
Bacterial Colonization ◽  
Control Measures ◽  
Clinical Infection ◽  
Antimicrobial Coating ◽  
Infection Control Measures ◽  
Surgical Implants ◽  
Clinical Protection ◽  
Coating Characteristics ◽  
The Impact

Device-associated infections are responsible for about half of nosocomial infections and can cause major medical and economical sequelae. Despite adherence to basic infection control measures, which constitute the mainstay for preventing infection, infections associated with certain devices continue to exist at unacceptably high rates. Potentially-preventive, antimicrobial-utilizing strategies include systemic antibiotic prophylaxis and local administration of antimicrobial agents (antibiotics or antiseptics), which includes antimicrobial irrigation of the surgical field, placement of antimicrobial carriers, antiseptic cleansing of the skin, dipping of surgical implants in antimicrobial solutions, and inserting antimicrobial-coated implants. Since bacterial colonization of the indwelling device is a prelude to infection, prevention of device colonization may lead to a lower rate of clinical infection. Different approaches for antimicrobial coating of devices have been variably successful in preventing device-associated infections. Optimal characteristics of antimicrobial coating can help predict the likelihood and degree of clinical protection against infection. This review addresses the impact of device-related infection, antimicrobial-utilizing approaches for preventing infection, clinical protection afforded by different types of antimicrobial coating, characteristics that predict the ability of antimicrobial coating of devices to prevent clinical infection, and future directions of antimicrobial coating.

Isolation and Antimicrobial Resistance ofStaphylococcus aureusIsolates in a Dental Clinic Environment

2007 ◽  
Vol 28 (2) ◽  
pp. 185-190 ◽  
Author(s):  
Rogério Heládio Lopes Motta ◽  
Francisco Carlos Groppo ◽  
Cristiane de Cássia Bergamaschi ◽  
Juliana Cama Ramacciato ◽  
Sinvaldo Baglie ◽  
...  
Keyword(s):  
Bacterial Resistance ◽  
Antimicrobial Agents ◽  
Brain Heart Infusion ◽  
Clinical Environment ◽  
Bacterial Strains ◽  
Biochemical Tests ◽  
Clinical Procedures ◽  
Gram Staining ◽  
Number Of Microorganisms ◽  
Antimicrobial Susceptibility Tests

Objective.To determine the number ofStaphylococcus aureusisolates collected in a dental clinical environment and to determine their susceptibility to antimicrobial agents commonly used in dentistry.Setting.Undergraduate clinic of the Dental School of Piracicaba, University of Campinas, Brazil.Methods.Sterile cotton swabs were used to collect the samples from dental-chair push buttons, light handles, 3-in-l syringes, computer “Enter” keys, doorknobs, and X-ray tubes before, during, and after clinical procedures. These samples were spread on brain-heart infusion agar and were incubated at 37°C for 24 hours. The resultingS. aureusisolates were counted and classified using Gram staining and biochemical tests. The counts among the 3 periods and the groups were analyzed by Kruskal-Wallis and Dunn tests (α= 5%). Commercial paper disks containing widely prescribed antimicrobial agents (β-lactams, macrolides, clindamycin, and vancomycin) were used to perform the antimicrobial susceptibility tests.Results.An increase in the number of microorganisms was observed during clinical procedures (P< .05). The highest bacterial resistance rates were observed for theβ-lactam group. All isolated strains were sensitive to vancomycin, and 2% of them were resistant to methicillin.Conclusions.Clinical procedures increased the number and proportion of antimicrobial-resistantS. aureusisolates dispersed in a dental clinical environment. The present study highlights the need to establish strategies to prevent emergence of drug-resistant bacterial strains in dental settings.

scholarly journals Evaluation of Antimicrobial Efficacy of Triphala Oral Spray in Patients Undergoing Orthodontic Treatment- A Randomized Clinical Trial

2021 ◽  
pp. 24-29
Author(s):  
Nishi Malviya ◽  
Nilima Thosar ◽  
Nilesh Rathi ◽  
Monika Khubchandani ◽  
V. G. Meshram
Keyword(s):  
Orthodontic Treatment ◽  
Antimicrobial Agents ◽  
Periodontal Diseases ◽  
Drug Formulation ◽  
Microbial Load ◽  
Antimicrobial Efficacy ◽  
Microbial Count ◽  
Oral Streptococci ◽  
Post Intervention ◽  
Plaque Score

Background: Malocclusion is one of the most susceptible causes of the development of periodontal diseases and dental caries in young individuals undergoing fixed orthodontic treatment. It provides a surface area for the accumulation of food particles, which further leads to plaque formation. Various mechanical and chemical aids are used regularly to decrease the microbial load and accumulation of plaque. Chemical adjuncts such as chlorhexidine mouthwash are widely used as antimicrobial agents that have significant efficacy against oral Streptococci and Actinomyces species. Herbal agents have replaced conventional medications due to the development of antibiotic resistance. Among herbal medicaments, Triphala is the well-known drug formulation that can be used as an adjuvant for commercially available chemical aid. Chlorhexidine mouth wash requires the assistance of the caregivers. Therefore, there is a need for a newer modality to maintain oral hygiene in patients undertaking orthodontic corrections. Objectives: 1. To evaluate the antimicrobial efficacy of Triphala oral spray on Streptococcus mutants levels in children undergoing orthodontic treatment. 2. To evaluate the efficacy of Triphala oral spray in reducing dental plaque in children undergoing orthodontic treatment. 3. To evaluate the efficacy of Triphala oral spray reducing gingivitis in children undergoing orthodontic treatment. Methodology: A randomized control trial will be done among – participants with the age group of 10 to 15 years of age. A total of 25 children were included in the study. The participants will be told to use the spray two times a day for one week. After 7 days, plaque index and the gingival index will be re-recorded, and plaque samples will be collected for post-intervention microbial analysis. Results: Triphala-based oral spray will effectively reduce plaque score, reduce gingival inflammation, and show inhibitory effects on microbial count. Conclusion: Triphala oral spray can be used as an adjuvant by the orthodontic patients for reduction of the microbial load. It also provides better acceptability by the children as it does not require any assistance of the caregivers.

COMPARISON OF MEDIA FOR THE ISOLATION OF FUSARIUM OXYSPORUM F. SP. LYCOPERSICI FROM SAWDUST USED FOR GROWING TOMATOES

1974 ◽  
Vol 54 (2) ◽  
pp. 373-374
Author(s):  
M. G. ANDERSON ◽  
R. G. ATKINSON
Keyword(s):  
Lycopersicon Esculentum ◽  
Fusarium Oxysporum ◽  
Rose Bengal ◽  
Antimicrobial Agents ◽  
Potato Dextrose Agar ◽  
Selective Isolation ◽  
Streptomycin Sulphate ◽  
Lycopersicon Esculentum Mill ◽  
Agar Media ◽  
Greenhouse Tomatoes

Various agar media amended with antimicrobial agents were compared for the selective isolation of Fusarium oxysporum f.sp. lycopersici from sawdust used for growing greenhouse tomatoes (Lycopersicon esculentum Mill.). Potato dextrose agar amended with pentachloronitrobenzene (PCNB) (500 mg/l) and streptomycin sulphate (300 mg/l proved to be the most satisfactory medium, tested. These antimicrobial agents, unlike rose bengal, did not reduce the number of Fusarium colonies isolated.

scholarly journals Microbial load of the urine in patients with recurrent urolithiasis and its correction

2020 ◽  
Vol 10 (1) ◽  
pp. 51-55
Author(s):  
Evgenii T. Goloshchapov ◽  
Andrey Valerevich Chetverikov
Keyword(s):  
High Frequency ◽  
Antimicrobial Agents ◽  
Stone Formation ◽  
Microbial Load ◽  
Spontaneous Discharge ◽  
Colloidal System ◽  
Colloidal Properties ◽  
The Stability ◽  
Quantitative Indicators

Among the causes of stone formation in the urinary system, an important role belongs to the microbiome, which affects the stability of the colloidal system of urine. Understanding the level of microbial tension in the urine allows to use pathogenetic approaches both for prevention and for the relapses of stone formation. The study included 162 patients with recurrent urolithiasis, 12 patients underwent complex anti-relapse therapy after surgical treatment (or spontaneous discharge of concretions after litokinetic therapy). It was determined that along with the known factors, there is a violation of quantitative indicators of urine microbiota. An increase of the microbial load of urine leads to a violation of the structure of uromodulin (Tamm Horsfall protein), which in turn determines the high frequency of recurrence of urinary lithogenesis. Thus, a comprehensive assessment of the urine microbiota and its impact on the state of uromodulin, lead to an improvement in the quality of urolithiasis metaphylaxis. In the complex of metaphylaxis and prevention of urolithiasis, it is advisable to use antimicrobial agents (uroseptics) not only to reduce the risk of infectious and inflammatory complications, but also to increase the stability of the colloidal properties of urine.

Light-Activated Antimicrobial Coating for the Continuous Disinfection of Surfaces

2003 ◽  
Vol 24 (10) ◽  
pp. 782-784 ◽  
Author(s):  
Michael Wilson
Keyword(s):  
Infectious Disease ◽  
Cellulose Acetate ◽  
White Light ◽  
Disease Transmission ◽  
Environmental Load ◽  
Infectious Disease Transmission ◽  
Cellulose Acetate Film ◽  
Antimicrobial Coating

AbstractThe ability of a photosensitizer-containing cellulose acetate film to kill bacteria was evaluated. Substantial kills were achieved following irradiation of the film with white light for up to 24 hours. Applying a photosensitizer-containing coating to surfaces could reduce the environmental load of pathogens, thus helping to prevent infectious disease transmission.

scholarly journals Antimicrobial Agents Used in Endodontic Treatment

2008 ◽  
Vol 51 (1) ◽  
pp. 3-12 ◽  
Author(s):  
Marina George Kudiyirickal ◽  
Romana Ivančaková
Keyword(s):  
Antimicrobial Agent ◽  
Mechanism Of Action ◽  
Root Canal ◽  
Antimicrobial Agents ◽  
Endodontic Treatment ◽  
Microbial Load ◽  
Potential Use

Biomechanical preparation alone does not completely eradicate microorganisms from the root canal, hence the next logical step is to perform root canal procedures in conjunction with antimicrobials. The use of an antimicrobial agent improves the efficacy and prognosis of endodontic treatment. This review enumerates the most widely used antimicrobial agents, their mechanism of action and their potential use in reducing the microbial load.

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