Viability inhibition of A375 melanoma cells in vitro by a high-frequency nanosecond-pulsed magnetic field combined with targeted iron oxide nanoparticles via membrane magnetoporation

The purpose of this study was to investigate the effectiveness in photodynamic therapy of iron oxide nanoparticles (γ-Fe2O3 NPs), synthesized by laser pyrolysis technique, functionalized with 5,10,15,20-(Tetra-4-sulfonatophenyl) porphyrin tetraammonium (TPPS) on human cutaneous melanoma cells, after only 1 min blue light exposure. The efficiency of porphyrin loading on the iron oxide nanocarriers was estimated by using absorption and FTIR spectroscopy. The singlet oxygen yield was determined via transient characteristics of singlet oxygen phosphorescence at 1270 nm both for porphyrin functionalized nanoparticles and rose bengal used as standard. The irradiation was performed with a LED (405 nm, 1 mW/cm2) for 1 min after melanoma cells were treated with TPPS functionalized iron oxide nanoparticles (γ-Fe2O3 NPs_TPPS) and incubated for 24 h. Biological tests revealed a high anticancer effect of γ-Fe2O3 NPs_TPPS complexes indi-cated by the inhibition of tumor cell proliferation, reduction of cell adhesion, and induction of cell death through ROS generated by TPPS under light exposure. The biological assays were combined with the pharmacokinetic prediction of the porphyrin.

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Assessing the combination of magnetic field stimulation, iron oxide nanoparticles, and aligned electrospun fibers for promoting neurite outgrowth from dorsal root ganglia in vitro.

Acta Biomaterialia ◽

10.1016/j.actbio.2021.06.049 ◽

2021 ◽

Author(s):

Jessica L. Funnell ◽

Alexis M. Ziemba ◽

James F. Nowak ◽

Hussein Awada ◽

Nicos Prokopiou ◽

...

Keyword(s):

Magnetic Field ◽

Iron Oxide ◽

Neurite Outgrowth ◽

Iron Oxide Nanoparticles ◽

Dorsal Root Ganglia ◽

Dorsal Root ◽

Electrospun Fibers ◽

Oxide Nanoparticles ◽

Field Stimulation

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Visualization and Motion of Curcumin Loaded Iron Oxide Nanoparticles During Magnetic Drug Targeting

Journal of Nanotechnology in Engineering and Medicine ◽

10.1115/1.4031062 ◽

2015 ◽

Vol 6 (1) ◽

Cited By ~ 4

Author(s):

Mohammed Asfer ◽

Ayodhya Prasad Prajapati ◽

Arun Kumar ◽

Pradipta Kumar Panigrahi

Keyword(s):

Magnetic Field ◽

Iron Oxide ◽

Iron Oxide Nanoparticles ◽

Applied Magnetic Field ◽

Drug Targeting ◽

In Vitro Study ◽

Target Site ◽

Magnetic Drug Targeting ◽

Oxide Nanoparticles

Magnetic drug targeting (MDT) involves the localization of drug loaded iron oxide nanoparticles (IONPs) around the malignant tissue using external magnetic field for therapeutic purposes. The present in vitro study reports the visualization and motion of curcumin loaded IONPs (CU-IONPs) around the target site inside a microcapillary (500 × 500 μm2 square cross section), in the presence of an externally applied magnetic field. Application of magnetic field leads to transportation and aggregation of CU-IONPs toward the target site inside the capillary adjacent to the magnet. The localization/aggregation of CU-IONPs at the target site shows strong dependence on the strength of the applied magnetic field and flow rate of ferrofluid through the capillary. Such an in vitro study offers a viable for optimization and design of MDT systems for in vivo applications.

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Polymer Coated Iron Nanoparticles: Radiolabeling & In Vitro Studies

Current Radiopharmaceuticals ◽

10.2174/1874471013666200430094113 ◽

2020 ◽

Vol 13 ◽

Author(s):

Selin Yılmaz ◽

Çiğdem İçhedef ◽

Kadriye Buşra Karatay ◽

Serap Teksöz

Keyword(s):

Breast Cancer ◽

Drug Delivery ◽

Iron Oxide ◽

Cancer Cells ◽

Iron Oxide Nanoparticles ◽

Breast Cancer Cells ◽

Cancer Drug ◽

Oxide Nanoparticles ◽

Sem Images

Backgorund: Superparamagnetic iron oxide nanoparticles (SPIONs) have been extensively used for targeted drug delivery systems due to their unique magnetic properties. Objective: In this study, it’s aimed to develop a novel targeted 99mTc radiolabeled polymeric drug delivery system for Gemcitabine (GEM). Methods: Gemcitabine, an anticancer agent, was encapsulated into polymer nanoparticles (PLGA) together with iron oxide nanoparticles via double emulsion technique and then labeled with 99mTc. SPIONs were synthesized by reduction–coprecipitation method and encapsulated with oleic acid for surface modification. Size distribution and the morphology of the synthesized nanoparticles were caharacterized by dynamic light scattering(DLS)and scanning electron microscopy(SEM), respectively. Radiolabeling yield of SPION-PLGAGEM nanoparticles were determined via Thin Layer Radio Chromatography (TLRC). Cytotoxicity of GEM loaded SPION-PLGA were investigated on MDA-MB-231 and MCF7 breast cancer cells in vitro. Results: SEM images displayed that the average size of the drug-free nanoparticles was 40 nm and the size of the drug-loaded nanoparticles was 50 nm. The diameter of nanoparticles were determined as 366.6 nm by DLS, while zeta potential was found as-29 mV. SPION was successfully coated with PLGA, which was confirmed by FTIR. GEM encapsulation efficiency of SPION-PLGA was calculated as 4±0.16 % by means of HPLC. Radiolabeling yield of SPION-PLGA-GEM nanoparticles were determined as 97.8±1.75 % via TLRC. Cytotoxicity of GEM loaded SPION-PLGA were investigated on MDA-MB-231 and MCF7 breast cancer cells. SPION-PLGA-GEM showed high uptake on MCF-7, whilst incorporation rate was increased for both cell lines which external magnetic field application. Conclusion: 99mTc labeled SPION-PLGA nanoparticles loaded with GEM may overcome some of the obstacles in anti-cancer drug delivery because of their appropriate size, non-toxic, and supermagnetic characteristics.

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Shaping and Cellular Uptake of Folic Acid Coated Gold and Magnetite Nanoparticles

Current Nanomedicine ◽

10.2174/2468187308666180913113827 ◽

2019 ◽

Vol 9 (2) ◽

pp. 166-172

Author(s):

Ahmed A.G. El-Shahawy ◽

Gamal Elghnam ◽

Alsayed A.M. Alsherbini

Keyword(s):

Folic Acid ◽

Iron Oxide ◽

Tumor Cells ◽

Iron Oxide Nanoparticles ◽

Individual Cell ◽

Oxide Nanoparticles ◽

Cellular Compartment ◽

Specific Tumor ◽

Uv Visible

Background:Gold and Iron Oxide nanoparticles NPs play as nanocarriers for a specific drug delivery and contrast agents. Intercellular uptake of these nanoparticles and targeting to individual cell and sub-cellular compartment is essential.Objective:The aim of the current study is to evaluate the intracellular uptake of these NPs to specific tumor cells in vitro conjugated with folic acid with a goal of enhancing the efficiency of specific targeting to tumor cells.Methods:We synthesized the nanoparticles by a chemical method and characterized by UV-Visible, FTIR, XRD, and TEM.Results & Conclusion:The results revealed the conjugation of Gold and Iron Oxide nanoparticles with folic acid increased the intercellular uptake with high percent compared to non- conjugated nanoparticles.

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Magnetic targeting with superparamagnetic iron oxide nanoparticles for in vivo glioma

Nanotechnology Reviews ◽

10.1515/ntrev-2016-0101 ◽

2017 ◽

Vol 6 (5) ◽

pp. 449-472 ◽

Cited By ~ 4

Author(s):

Marina Fontes de Paula Aguiar ◽

Javier Bustamante Mamani ◽

Taylla Klei Felix ◽

Rafael Ferreira dos Reis ◽

Helio Rodrigues da Silva ◽

...

Keyword(s):

Magnetic Field ◽

Iron Oxide ◽

Iron Oxide Nanoparticles ◽

Superparamagnetic Iron Oxide ◽

Superparamagnetic Iron Oxide Nanoparticles ◽

Process Efficiency ◽

Cochrane Library ◽

Oxide Nanoparticles ◽

Magnetic Targeting

AbstractThe purpose of this study was to review the use of the magnetic targeting technique, characterized by magnetic driving compounds based on superparamagnetic iron oxide nanoparticles (SPIONs), as drug delivery for a specific brain locus in gliomas. We reviewed a process mediated by the application of an external static magnetic field for targeting SPIONs in gliomas. A search of PubMed, Cochrane Library, Scopus, and Web of Science databases identified 228 studies, 23 of which were selected based on inclusion criteria and predetermined exclusion criteria. The articles were analyzed by physicochemical characteristics of SPIONs used, cell types used for tumor induction, characteristics of experimental glioma models, magnetic targeting technical parameters, and analysis method of process efficiency. The study shows the highlights and importance of magnetic targeting to optimize the magnetic targeting process as a therapeutic strategy for gliomas. Regardless of the intensity of the patterned magnetic field, the time of application of the field, and nanoparticle used (commercial or synthesized), all studies showed a vast advantage in the use of magnetic targeting, either alone or in combination with other techniques, for optimized glioma therapy. Therefore, this review elucidates the preclinical and therapeutic applications of magnetic targeting in glioma, an innovative nanobiotechnological method.

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